研究通过对番茄MSI2-like蛋白序列与其它模式植物的蛋白序列进行同源性比对,发现番茄MSI2-like与马铃薯的同源性最高;另外还预测了其蛋白质的理化性质及三级结构.酵母双杂交实验证明,MSI2-like与UV-Damaged DNA Binding Protein 1(DDB1...研究通过对番茄MSI2-like蛋白序列与其它模式植物的蛋白序列进行同源性比对,发现番茄MSI2-like与马铃薯的同源性最高;另外还预测了其蛋白质的理化性质及三级结构.酵母双杂交实验证明,MSI2-like与UV-Damaged DNA Binding Protein 1(DDB1)具有相互作用.同时,利用RNA干涉技术构建植物双元载体,农杆菌介导法转化番茄得到转基因植株,该转基因植株在干旱胁迫条件下有一定的抗旱功能.再利用实时荧光定量PCR分析MSI2-like基因表达的组织差异性,发现它在各个组织中都表达,但是在生殖器官花中的表达量明显较其他组织高.展开更多
目的 探寻胃癌组织中Musashi2（MSI2）表达水平及与其临床病理学特征的关系。方法 应用实时定量PCR方法检测67例胃癌患者MSI2 m RNA在肿瘤和癌旁组织中的表达水平,用Western blot法验证其高表达者MSI2蛋白表达水平;分析其表达水平和胃...目的 探寻胃癌组织中Musashi2（MSI2）表达水平及与其临床病理学特征的关系。方法 应用实时定量PCR方法检测67例胃癌患者MSI2 m RNA在肿瘤和癌旁组织中的表达水平,用Western blot法验证其高表达者MSI2蛋白表达水平;分析其表达水平和胃癌临床病理学特征的关系,并分析比较MSI2 m RNA高表达者（胃癌组织与相应癌旁组织中MSI2 m RNA表达水平之比≥2倍）和MSI2 m RNA低表达者（胃癌组织与相应癌旁组织中MSI2 m RNA表达水平之比〈2倍）的预后情况。结果 MSI2 m RNA在胃癌组织和癌旁组织间的表达水平差异无统计学意义（P〉0.05）。MSI2 m RNA表达水平与肿瘤浸润深度（P=0.017）、TNM分期（P=0.028）、分化程度（P=0.020）及肿瘤大小（P=0.030）相关,与患者的性别、年龄及肿瘤部位无关（P〉0.05）。MSI2 m RNA高表达者的总体生存率明显低于低表达者（χ^2=4.221,P=0.040）。结论 MSI2表达水平与胃癌的侵袭、分期和分化程度相关,高表达者预后较差。MSI2可能作为未来胃癌治疗的新的分子靶点。展开更多
It has been suggested that altered neurogenesis may be involved in the etiology of schizophrenia,so genes impacting on neurogenesis could be potential candidates for schizophrenia.A member of the Musashi family,the hu...It has been suggested that altered neurogenesis may be involved in the etiology of schizophrenia,so genes impacting on neurogenesis could be potential candidates for schizophrenia.A member of the Musashi family,the human MSI2 gene plays a substantial role in stem-cell maintenance,asymmetric division,and differentiation during neurogenesis.Our previous genome-wide association study（GWAS）implied an association of MSI2 with schizophrenia in a Han Chinese population.To further explore this association,three single-nucleotide polymorphisms（SNPs）,rs9892791,rs11657292,and rs1822381,were selected for a replication study involving 921 schizophrenia cases and 1244 controls.After rigorous Bonferroni correction,two of the SNPs（rs9892791 and rs11657292） displayed significant differences in allele and genotype distribution frequencies between the case and control groups.When our GWAS and replication samples were combined,the three MSI2 SNPs were all strongly associated with schizophrenia（rs9892791：allelic P = 1.07E-5;rs11657292：allelic P = 1.95E-12;rs1822381：allelic P = 1.44E-4）.These results indicate that the human MSI2 gene might be a susceptibility gene forschizophrenia and encourage future research on the functional relationship between this gene and schizophrenia.展开更多
The burning of crop residues in fields is a significant global biomass burning activity which is a key element of the terrestrial carbon cycle,and an important source of atmospheric trace gasses and aerosols.Accurate ...The burning of crop residues in fields is a significant global biomass burning activity which is a key element of the terrestrial carbon cycle,and an important source of atmospheric trace gasses and aerosols.Accurate estimation of cropland burned area is both crucial and challenging,especially for the small and fragmented burned scars in China.Here we developed an automated burned area mapping algorithm that was implemented using Sentinel-2 Multi Spectral Instrument(MSI)data and its effectiveness was tested taking Songnen Plain,Northeast China as a case using satellite image of 2020.We employed a logistic regression method for integrating multiple spectral data into a synthetic indicator,and compared the results with manually interpreted burned area reference maps and the Moderate-Resolution Imaging Spectroradiometer(MODIS)MCD64A1 burned area product.The overall accuracy of the single variable logistic regression was 77.38%to 86.90%and 73.47%to 97.14%for the 52TCQ and 51TYM cases,respectively.In comparison,the accuracy of the burned area map was improved to 87.14%and 98.33%for the 52TCQ and 51TYM cases,respectively by multiple variable logistic regression of Sentind-2 images.The balance of omission error and commission error was also improved.The integration of multiple spectral data combined with a logistic regression method proves to be effective for burned area detection,offering a highly automated process with an automatic threshold determination mechanism.This method exhibits excellent extensibility and flexibility taking the image tile as the operating unit.It is suitable for burned area detection at a regional scale and can also be implemented with other satellite data.展开更多
文摘研究通过对番茄MSI2-like蛋白序列与其它模式植物的蛋白序列进行同源性比对,发现番茄MSI2-like与马铃薯的同源性最高;另外还预测了其蛋白质的理化性质及三级结构.酵母双杂交实验证明,MSI2-like与UV-Damaged DNA Binding Protein 1(DDB1)具有相互作用.同时,利用RNA干涉技术构建植物双元载体,农杆菌介导法转化番茄得到转基因植株,该转基因植株在干旱胁迫条件下有一定的抗旱功能.再利用实时荧光定量PCR分析MSI2-like基因表达的组织差异性,发现它在各个组织中都表达,但是在生殖器官花中的表达量明显较其他组织高.
文摘目的 探寻胃癌组织中Musashi2（MSI2）表达水平及与其临床病理学特征的关系。方法 应用实时定量PCR方法检测67例胃癌患者MSI2 m RNA在肿瘤和癌旁组织中的表达水平,用Western blot法验证其高表达者MSI2蛋白表达水平;分析其表达水平和胃癌临床病理学特征的关系,并分析比较MSI2 m RNA高表达者（胃癌组织与相应癌旁组织中MSI2 m RNA表达水平之比≥2倍）和MSI2 m RNA低表达者（胃癌组织与相应癌旁组织中MSI2 m RNA表达水平之比〈2倍）的预后情况。结果 MSI2 m RNA在胃癌组织和癌旁组织间的表达水平差异无统计学意义（P〉0.05）。MSI2 m RNA表达水平与肿瘤浸润深度（P=0.017）、TNM分期（P=0.028）、分化程度（P=0.020）及肿瘤大小（P=0.030）相关,与患者的性别、年龄及肿瘤部位无关（P〉0.05）。MSI2 m RNA高表达者的总体生存率明显低于低表达者（χ^2=4.221,P=0.040）。结论 MSI2表达水平与胃癌的侵袭、分期和分化程度相关,高表达者预后较差。MSI2可能作为未来胃癌治疗的新的分子靶点。
文摘It has been suggested that altered neurogenesis may be involved in the etiology of schizophrenia,so genes impacting on neurogenesis could be potential candidates for schizophrenia.A member of the Musashi family,the human MSI2 gene plays a substantial role in stem-cell maintenance,asymmetric division,and differentiation during neurogenesis.Our previous genome-wide association study（GWAS）implied an association of MSI2 with schizophrenia in a Han Chinese population.To further explore this association,three single-nucleotide polymorphisms（SNPs）,rs9892791,rs11657292,and rs1822381,were selected for a replication study involving 921 schizophrenia cases and 1244 controls.After rigorous Bonferroni correction,two of the SNPs（rs9892791 and rs11657292） displayed significant differences in allele and genotype distribution frequencies between the case and control groups.When our GWAS and replication samples were combined,the three MSI2 SNPs were all strongly associated with schizophrenia（rs9892791：allelic P = 1.07E-5;rs11657292：allelic P = 1.95E-12;rs1822381：allelic P = 1.44E-4）.These results indicate that the human MSI2 gene might be a susceptibility gene forschizophrenia and encourage future research on the functional relationship between this gene and schizophrenia.
基金Under the auspices of National Natural Science Foundation of China(No.42101414)Natural Science Found for Outstanding Young Scholars in Jilin Province(No.20230508106RC)。
文摘The burning of crop residues in fields is a significant global biomass burning activity which is a key element of the terrestrial carbon cycle,and an important source of atmospheric trace gasses and aerosols.Accurate estimation of cropland burned area is both crucial and challenging,especially for the small and fragmented burned scars in China.Here we developed an automated burned area mapping algorithm that was implemented using Sentinel-2 Multi Spectral Instrument(MSI)data and its effectiveness was tested taking Songnen Plain,Northeast China as a case using satellite image of 2020.We employed a logistic regression method for integrating multiple spectral data into a synthetic indicator,and compared the results with manually interpreted burned area reference maps and the Moderate-Resolution Imaging Spectroradiometer(MODIS)MCD64A1 burned area product.The overall accuracy of the single variable logistic regression was 77.38%to 86.90%and 73.47%to 97.14%for the 52TCQ and 51TYM cases,respectively.In comparison,the accuracy of the burned area map was improved to 87.14%and 98.33%for the 52TCQ and 51TYM cases,respectively by multiple variable logistic regression of Sentind-2 images.The balance of omission error and commission error was also improved.The integration of multiple spectral data combined with a logistic regression method proves to be effective for burned area detection,offering a highly automated process with an automatic threshold determination mechanism.This method exhibits excellent extensibility and flexibility taking the image tile as the operating unit.It is suitable for burned area detection at a regional scale and can also be implemented with other satellite data.
