Objectives To investigate the correlation between sex hormones(SH) and aging. Methods Through epidemiological investigation in our country, the levels of SH were measured by radioimmunoassy; lipid profile, glucose and...Objectives To investigate the correlation between sex hormones(SH) and aging. Methods Through epidemiological investigation in our country, the levels of SH were measured by radioimmunoassy; lipid profile, glucose and apolipoprotein by automatic biochemic analytical instrument; T cell subsets by flow cytometer; and MDA, SOD were evaluated by the thiobarbituric acid (TBA) test and the nitrite method modified by Oyanagui respectively using spectrophotometry. Results In men, the serum levels of follicle stimulating hormone ( FSH)、luteinizing hormone(LH) increased significantly with aging, but serum prolactin(PRL) and progesterone(P) levels remained unchanged in all life; Both testosterone (T) and free testosterone (FT) all decreased greatly with aging, but 17β - estradiol( 17β - E_2) was reverse ; E_2 was negatively correlated with T and E_2/T increased with aging. The level of serum total cholesterol (TC) increased with aging, but triglycerides (TG) remain unchanged; compared with young group, high - density lipoprotein cholesterol ( HDL - C) ; HDL - C/TC of other groups decreased significantly, but low - density lipoprotein cholesterol ( LDL - C ) changed inversely; HDL- C/LDL- C reduced slightly with aging and showed no difference between groups. Apolipoprotein A1 (apoA1) and apoB all enhanced greatly with aging; meanwhile the ratio of apoA1/apoB decreased. The concentration of serum glucose (GLU) was unchanged in all life. To compare with those in the young group, CD3 + , CD4 + in other groups reduced greatly, CD4 + remained unchanged. Meanwhile, CD8 + increased significantly with aging. Compared with the young group, serum malondialdehyde(MDA) value of the old ones increased obviously, but the activity of superoxide dismutase(SOD) was reverse. By partial correlation analysis (controlling BMI, FSH, LH and PRL), TC、 LDL-C、apoA1、apoB、CD8 + 、MDA of men all presented a positive correlation with E_2/T respectively, their correlation coefficients (γ) were 0. 262、0. 136、 0. 532、0. 379、0. 394、0. 234 (P < 0. 001 ) ; HDL - C、 HDL- C/TC、HDL- C/LDL- C、CD3 + 、CD4 +/CD8 + 、SOD showed a negatively correlation with E_2/T respectively, γequaled - 0.563、- 0.332、- 0.654、- 0.1530、-0.4140、-0.236(P<0.001). In women, the serum concentrations of FSH、LH increased significantly after menopause; PRL increased little with aging; compared with young group, E_2 and P in postmenopausal groups reduced obviously, E_2/P revealed significant reduce with aging. T enhanced significantly after menopause, but nor did FT. E_2, P and the ratio of E_2/P were negatively correlated with age respectively by bivariate correlation analysis, and a positive relation between T and age. After 70 years old, the level of TC increased obviously, and so did that of TG after menopause; HDL decreased with aging, but LDL increased after 70, with the result that the ratios of HDL- C/TC and HDL- C/LDL- C all reduced with aging; apoA1 decreased gently after 70, but apoB increased signifi- cantly after menopause; correspondingly, the ratio of apoA1/apoB declined obviously. The concentration of GLU increased with aging. CD3 + and CD4 + didn't change until 60, but reduced after 60. Compared with the young groups, CD8 + remained unchanged, CD4 +/CD8 + reduced greatly with aging, CD4 + and CD8 + presented a negatively correlation with age respectively. The value of MDA in serum of women increased notably after 70 years old, but SOD activity already decreased significantly from 60. By partial correlation analysis (controlling BMI, FSH, LH and PRL), HDL-C、CD4 +、CD4 +/CD8 + showed a certain correlation with E_2/P respectively; γ were 0. 245、 0.157、0. 154 ( P <0.05 ) ; TG、 LDL、 apoB、 apoA1/ apoB、SOD presented a negatively correlation with E_2/P respectively, γ were 0. 452、 0. 236、 0.321、 0. 135、 0.156、0.154、0.426 ( P < 0.05 ). Conclusions The Disequilibrium of SH had correlations with lipid profile, cellular immunological function and lipid peroxides and oxidation system; these suggested SH took an important role in the process of aging.展开更多
目的了解青年学生中男男性行为人群(men who have sex with men,MSM)HIV、梅毒感染率及估计HIV发病率趋势状况。方法分析2006-2009年青年学生中男男性行为人群调查资料,比较分析HIV、梅毒感染率以及估计的HIV发病率的趋势。估计HIV发病...目的了解青年学生中男男性行为人群(men who have sex with men,MSM)HIV、梅毒感染率及估计HIV发病率趋势状况。方法分析2006-2009年青年学生中男男性行为人群调查资料,比较分析HIV、梅毒感染率以及估计的HIV发病率的趋势。估计HIV发病率=HIV阳性数/[(年龄-与男性发生首次性行为的年龄)之和]。结果 2006-2009年分别调查了青年学生MSM人群210、206、181人和190人,最近1次与男性肛交时安全套使用率由2006年的48.0%上升至2009年的64.3%,最近6个月与男性发生无保护性肛交的比例由2006年的71.1%下降至2009年的65.3%。2006-2009年HIV感染率为4.3%、3.9%、11.0%、11.1%,梅毒感染率为7.1%、3.9%、2.8%、5.8%,对22岁及以下年龄组的估计发病率为1.4%、0.9%、2.4%和2.5%。结论重庆市MSM人群中青年学生HIV感染率高并呈上升的趋势,估计发病率也呈上升的趋势,流行形势严峻,急需针对该人群开展艾滋病防治工作。展开更多
目的了解男男性接触者(men who have sex with men,MSM)不安全性行为状况及其影响因素,为开展有针对性的干预工作提供建议。方法通过滚雪球的方式招募MSM进行面对面的问卷调查。以不安全性行为为应变量,人口学特征、艾滋病防治知识知晓...目的了解男男性接触者(men who have sex with men,MSM)不安全性行为状况及其影响因素,为开展有针对性的干预工作提供建议。方法通过滚雪球的方式招募MSM进行面对面的问卷调查。以不安全性行为为应变量,人口学特征、艾滋病防治知识知晓水平、性行为状况、接受干预服务等为自变量进行逐步向前法的logistic回归分析与不安全性行为的因素。结果调查的1 044名MSM中,艾滋病防治知识知晓率高(89.5%),近6个月与男性发生无保护性的肛交比例为63.8%。多因素回归分析发现,在婚、文化程度低、干部职员、待业人员、艾滋病防治知识认知水平差、过去1年未接受干预服务是影响不安全性行为的独立危险因素。结论无保护性性行为的发生将促进艾滋病在MSM人群中的传播,开展有针对性的干预和扩大干预工作的覆盖面是当前MSM人群艾滋病防治工作的重点。展开更多
目的了解男男性行为人群(man who have sex with men,MSM)人群中HIV感染者的特征及艾滋病相关因素的变化趋势,为MSM人群干预提供针对性的建议。方法对2006-2009年4次横断面调查发现的HIV感染者的资料进行统计分析,比较趋势变化。结果 20...目的了解男男性行为人群(man who have sex with men,MSM)人群中HIV感染者的特征及艾滋病相关因素的变化趋势,为MSM人群干预提供针对性的建议。方法对2006-2009年4次横断面调查发现的HIV感染者的资料进行统计分析,比较趋势变化。结果 2006-2009年发现的HIV感染者呈现低龄化,未婚比例、大专及以上文化程度感染者的比例及学生所占比例呈上升的趋势。最近1次与男性发生肛交时安全套使用率由2006年的48.4%上升至2009年的68.0%,最近6个月与男性发生肛交时每次都使用安全套的比例由2006年的17.2%上升至2007年的34.9%(P=0.016),梅毒感染率由2006年的16.3%上升至2009年的27.0%。2006-2009年接受干预服务的比例均低于40%。结论 MSM人群中的HIV感染者呈现低龄化、安全套使用率及接受干预服务比例低,合并梅毒感染率高,急需对MSM人群及其感染者开展更具针对性的干预工作。展开更多
目的了解2013年在广东省新发现的男男性行为（man have sex with man,MSM）人类免疫缺陷病毒-Ⅰ（human immunodeficiency virus 1,HIV-1）感染者中基因型耐药情况及亚型分布。方法采用In-House方法,扩增HIV-1 pol基因蛋白酶区（proteas...目的了解2013年在广东省新发现的男男性行为（man have sex with man,MSM）人类免疫缺陷病毒-Ⅰ（human immunodeficiency virus 1,HIV-1）感染者中基因型耐药情况及亚型分布。方法采用In-House方法,扩增HIV-1 pol基因蛋白酶区（protease region,PR）基因全长和逆转录酶区（reverse transcriptase region,RT）基因部分片段,通过HIV-1耐药数据库,获得个体耐药突变位点、基因亚型及药物耐受情况。结果在203人中,HIV-1病毒载量拷贝数中值为4.27×10~4拷贝/mL,平均值拷贝数为9.37×10~4拷贝/mL;高度基因型耐药病例2人,占1.2%;中度耐药1人,占0.6%;低度耐药7人,占4.1%;潜在耐药26人,占15.4%。PR区未见主要耐药突变;RT区主要耐药突变有：K70R、M184V、T215IT、K219E、K103KN、V106I、E138EG、V179E/D;出现了针对核苷类逆转录酶抑制剂3TC及FTC的高度耐药1例,占0.6%;针对非核苷类逆转录酶抑制剂EFV及NVP高度耐药1例,占0.6%;亚型方面,主要以CRF01_AE及CRF_BC重组亚型为主,同时B亚型在MSM人群中占20.1%。结论广东省目前MSM感染者中HIV-1高度耐药毒株尚处于低流行,潜在耐药毒株可能已经在人群中传播;MSM人群中以CRF01_AE、CRF_BC重组亚型及B亚型毒株流行为主。展开更多
文摘Objectives To investigate the correlation between sex hormones(SH) and aging. Methods Through epidemiological investigation in our country, the levels of SH were measured by radioimmunoassy; lipid profile, glucose and apolipoprotein by automatic biochemic analytical instrument; T cell subsets by flow cytometer; and MDA, SOD were evaluated by the thiobarbituric acid (TBA) test and the nitrite method modified by Oyanagui respectively using spectrophotometry. Results In men, the serum levels of follicle stimulating hormone ( FSH)、luteinizing hormone(LH) increased significantly with aging, but serum prolactin(PRL) and progesterone(P) levels remained unchanged in all life; Both testosterone (T) and free testosterone (FT) all decreased greatly with aging, but 17β - estradiol( 17β - E_2) was reverse ; E_2 was negatively correlated with T and E_2/T increased with aging. The level of serum total cholesterol (TC) increased with aging, but triglycerides (TG) remain unchanged; compared with young group, high - density lipoprotein cholesterol ( HDL - C) ; HDL - C/TC of other groups decreased significantly, but low - density lipoprotein cholesterol ( LDL - C ) changed inversely; HDL- C/LDL- C reduced slightly with aging and showed no difference between groups. Apolipoprotein A1 (apoA1) and apoB all enhanced greatly with aging; meanwhile the ratio of apoA1/apoB decreased. The concentration of serum glucose (GLU) was unchanged in all life. To compare with those in the young group, CD3 + , CD4 + in other groups reduced greatly, CD4 + remained unchanged. Meanwhile, CD8 + increased significantly with aging. Compared with the young group, serum malondialdehyde(MDA) value of the old ones increased obviously, but the activity of superoxide dismutase(SOD) was reverse. By partial correlation analysis (controlling BMI, FSH, LH and PRL), TC、 LDL-C、apoA1、apoB、CD8 + 、MDA of men all presented a positive correlation with E_2/T respectively, their correlation coefficients (γ) were 0. 262、0. 136、 0. 532、0. 379、0. 394、0. 234 (P < 0. 001 ) ; HDL - C、 HDL- C/TC、HDL- C/LDL- C、CD3 + 、CD4 +/CD8 + 、SOD showed a negatively correlation with E_2/T respectively, γequaled - 0.563、- 0.332、- 0.654、- 0.1530、-0.4140、-0.236(P<0.001). In women, the serum concentrations of FSH、LH increased significantly after menopause; PRL increased little with aging; compared with young group, E_2 and P in postmenopausal groups reduced obviously, E_2/P revealed significant reduce with aging. T enhanced significantly after menopause, but nor did FT. E_2, P and the ratio of E_2/P were negatively correlated with age respectively by bivariate correlation analysis, and a positive relation between T and age. After 70 years old, the level of TC increased obviously, and so did that of TG after menopause; HDL decreased with aging, but LDL increased after 70, with the result that the ratios of HDL- C/TC and HDL- C/LDL- C all reduced with aging; apoA1 decreased gently after 70, but apoB increased signifi- cantly after menopause; correspondingly, the ratio of apoA1/apoB declined obviously. The concentration of GLU increased with aging. CD3 + and CD4 + didn't change until 60, but reduced after 60. Compared with the young groups, CD8 + remained unchanged, CD4 +/CD8 + reduced greatly with aging, CD4 + and CD8 + presented a negatively correlation with age respectively. The value of MDA in serum of women increased notably after 70 years old, but SOD activity already decreased significantly from 60. By partial correlation analysis (controlling BMI, FSH, LH and PRL), HDL-C、CD4 +、CD4 +/CD8 + showed a certain correlation with E_2/P respectively; γ were 0. 245、 0.157、0. 154 ( P <0.05 ) ; TG、 LDL、 apoB、 apoA1/ apoB、SOD presented a negatively correlation with E_2/P respectively, γ were 0. 452、 0. 236、 0.321、 0. 135、 0.156、0.154、0.426 ( P < 0.05 ). Conclusions The Disequilibrium of SH had correlations with lipid profile, cellular immunological function and lipid peroxides and oxidation system; these suggested SH took an important role in the process of aging.
文摘目的了解青年学生中男男性行为人群(men who have sex with men,MSM)HIV、梅毒感染率及估计HIV发病率趋势状况。方法分析2006-2009年青年学生中男男性行为人群调查资料,比较分析HIV、梅毒感染率以及估计的HIV发病率的趋势。估计HIV发病率=HIV阳性数/[(年龄-与男性发生首次性行为的年龄)之和]。结果 2006-2009年分别调查了青年学生MSM人群210、206、181人和190人,最近1次与男性肛交时安全套使用率由2006年的48.0%上升至2009年的64.3%,最近6个月与男性发生无保护性肛交的比例由2006年的71.1%下降至2009年的65.3%。2006-2009年HIV感染率为4.3%、3.9%、11.0%、11.1%,梅毒感染率为7.1%、3.9%、2.8%、5.8%,对22岁及以下年龄组的估计发病率为1.4%、0.9%、2.4%和2.5%。结论重庆市MSM人群中青年学生HIV感染率高并呈上升的趋势,估计发病率也呈上升的趋势,流行形势严峻,急需针对该人群开展艾滋病防治工作。
文摘目的了解男男性接触者(men who have sex with men,MSM)不安全性行为状况及其影响因素,为开展有针对性的干预工作提供建议。方法通过滚雪球的方式招募MSM进行面对面的问卷调查。以不安全性行为为应变量,人口学特征、艾滋病防治知识知晓水平、性行为状况、接受干预服务等为自变量进行逐步向前法的logistic回归分析与不安全性行为的因素。结果调查的1 044名MSM中,艾滋病防治知识知晓率高(89.5%),近6个月与男性发生无保护性的肛交比例为63.8%。多因素回归分析发现,在婚、文化程度低、干部职员、待业人员、艾滋病防治知识认知水平差、过去1年未接受干预服务是影响不安全性行为的独立危险因素。结论无保护性性行为的发生将促进艾滋病在MSM人群中的传播,开展有针对性的干预和扩大干预工作的覆盖面是当前MSM人群艾滋病防治工作的重点。
文摘目的了解男男性行为人群(man who have sex with men,MSM)人群中HIV感染者的特征及艾滋病相关因素的变化趋势,为MSM人群干预提供针对性的建议。方法对2006-2009年4次横断面调查发现的HIV感染者的资料进行统计分析,比较趋势变化。结果 2006-2009年发现的HIV感染者呈现低龄化,未婚比例、大专及以上文化程度感染者的比例及学生所占比例呈上升的趋势。最近1次与男性发生肛交时安全套使用率由2006年的48.4%上升至2009年的68.0%,最近6个月与男性发生肛交时每次都使用安全套的比例由2006年的17.2%上升至2007年的34.9%(P=0.016),梅毒感染率由2006年的16.3%上升至2009年的27.0%。2006-2009年接受干预服务的比例均低于40%。结论 MSM人群中的HIV感染者呈现低龄化、安全套使用率及接受干预服务比例低,合并梅毒感染率高,急需对MSM人群及其感染者开展更具针对性的干预工作。
文摘目的了解2013年在广东省新发现的男男性行为（man have sex with man,MSM）人类免疫缺陷病毒-Ⅰ（human immunodeficiency virus 1,HIV-1）感染者中基因型耐药情况及亚型分布。方法采用In-House方法,扩增HIV-1 pol基因蛋白酶区（protease region,PR）基因全长和逆转录酶区（reverse transcriptase region,RT）基因部分片段,通过HIV-1耐药数据库,获得个体耐药突变位点、基因亚型及药物耐受情况。结果在203人中,HIV-1病毒载量拷贝数中值为4.27×10~4拷贝/mL,平均值拷贝数为9.37×10~4拷贝/mL;高度基因型耐药病例2人,占1.2%;中度耐药1人,占0.6%;低度耐药7人,占4.1%;潜在耐药26人,占15.4%。PR区未见主要耐药突变;RT区主要耐药突变有：K70R、M184V、T215IT、K219E、K103KN、V106I、E138EG、V179E/D;出现了针对核苷类逆转录酶抑制剂3TC及FTC的高度耐药1例,占0.6%;针对非核苷类逆转录酶抑制剂EFV及NVP高度耐药1例,占0.6%;亚型方面,主要以CRF01_AE及CRF_BC重组亚型为主,同时B亚型在MSM人群中占20.1%。结论广东省目前MSM感染者中HIV-1高度耐药毒株尚处于低流行,潜在耐药毒株可能已经在人群中传播;MSM人群中以CRF01_AE、CRF_BC重组亚型及B亚型毒株流行为主。
