Acutely isolated mouse hippocampal CA3 pyramidal neurons were exposed to 3 mT static magnetic field,and the characteristics of transient outward K+ channel were studied using the whole-cell patch-clamp technique.The e...Acutely isolated mouse hippocampal CA3 pyramidal neurons were exposed to 3 mT static magnetic field,and the characteristics of transient outward K+ channel were studied using the whole-cell patch-clamp technique.The experiment revealed that the amplitude of transient outward potassium channel current was reduced.The maximum activated current densities of control group and exposure group were 163.62±20.68 pA/pF and 98.74±16.57 pA/pF(n=12,P<0.01) respectively.The static magnetic field exposure affected the activation and inactivation process of transient outward potassium channel current.Due to the magnetic field exposure,the half-activation voltage of the activation curves changed from 5.59±1.96 mV to 27.87±7.24 mV(n=12,P<0.05) ,and the slope factor changed from 19.43±2.11 mV to 25.87±4.22 mV(n=12,P<0.05) .The half-inactivation voltage of the inactivation curves also changed from-56.09±0.89 mV to-57.16±1.10 mV(n=12,P>0.05) and the slope factor of the inactivation curves from 8.69±0.80 mV to 10.87±1.02 mV(n=12,P<0.05) .The results show that the static magnetic field can change the characteristics of transient outward K+ channel,and affect the physiological functions of neurons.展开更多
本研究以CiteSpace V为工具,以2008—2016年间Web of Science收录的十大国际翻译研究权威期刊2452篇翻译技术文献引文为研究对象,采取可视化图谱分析方法,分析国外翻译技术研究热点及发展趋势。研究发现:2008—2016年间,国外翻译技术理...本研究以CiteSpace V为工具,以2008—2016年间Web of Science收录的十大国际翻译研究权威期刊2452篇翻译技术文献引文为研究对象,采取可视化图谱分析方法,分析国外翻译技术研究热点及发展趋势。研究发现:2008—2016年间,国外翻译技术理论和应用研究基本趋于均衡,2010—2012年研究成果最为丰富,应用研究集中分布在医学保健、教育、经济、政治和心理学领域,未来研究趋势是在社会学、生态学、生物学等多学科交叉中实现翻译技术与大数据融合。展开更多
基金Supported by National Natural Science Foundation of China(No. 60674111)
文摘Acutely isolated mouse hippocampal CA3 pyramidal neurons were exposed to 3 mT static magnetic field,and the characteristics of transient outward K+ channel were studied using the whole-cell patch-clamp technique.The experiment revealed that the amplitude of transient outward potassium channel current was reduced.The maximum activated current densities of control group and exposure group were 163.62±20.68 pA/pF and 98.74±16.57 pA/pF(n=12,P<0.01) respectively.The static magnetic field exposure affected the activation and inactivation process of transient outward potassium channel current.Due to the magnetic field exposure,the half-activation voltage of the activation curves changed from 5.59±1.96 mV to 27.87±7.24 mV(n=12,P<0.05) ,and the slope factor changed from 19.43±2.11 mV to 25.87±4.22 mV(n=12,P<0.05) .The half-inactivation voltage of the inactivation curves also changed from-56.09±0.89 mV to-57.16±1.10 mV(n=12,P>0.05) and the slope factor of the inactivation curves from 8.69±0.80 mV to 10.87±1.02 mV(n=12,P<0.05) .The results show that the static magnetic field can change the characteristics of transient outward K+ channel,and affect the physiological functions of neurons.
文摘本研究以CiteSpace V为工具,以2008—2016年间Web of Science收录的十大国际翻译研究权威期刊2452篇翻译技术文献引文为研究对象,采取可视化图谱分析方法,分析国外翻译技术研究热点及发展趋势。研究发现:2008—2016年间,国外翻译技术理论和应用研究基本趋于均衡,2010—2012年研究成果最为丰富,应用研究集中分布在医学保健、教育、经济、政治和心理学领域,未来研究趋势是在社会学、生态学、生物学等多学科交叉中实现翻译技术与大数据融合。
