MT-MMP-1在大肠癌中的表达及其意义

为探讨膜型基质金属蛋白酶-1（MT-MMP-1）在大肠癌中的表达及其与大肠癌侵袭、转移及预后的关系，采用免疫组织化学SP法检测136例大肠癌组织及20例正常大肠黏膜组织中MT-MMP-1的表达，并分析其与大肠癌临床病理因素的关系。MT-MMP-1蛋白在136例大肠癌组织中的阳性表达率为74．3％（101／136），20例正常大肠黏膜中的阳性表达率为0（P〈O，01）；在UICC2002Ⅰ＋Ⅱ与Ⅲ＋Ⅳ期的阳性表达率分别为52．3％和84．8％（P〈0．01）；在T1＋T2与T3＋T4两组的阳性表达率分别为65．1％和82．2％（P〈0．05）；在N0与N1＋N2两组的阳性表达率分别为47．7％和87％（P〈0．01）；在M0与M1两组的阳性表达率为70．9％和94．7％（P〈0．05）。结果表明。MT-MMP-1蛋白在大肠癌中的表达与肿瘤分期、肿瘤大小、淋巴结转移和器官转移有关，在大肠癌的侵袭、转移中发挥重要作用。

Localization and possible role of membrane type metallo-proteinase and tissue inhibitors of metalloproteinase-1 in early stages of placentation

Human placental tissues from the first and second trimesters of gestation have been investigated using riboprobe in situ hybridisation of mRNA sequences coding for membrane type metalloproteinase (MT-1-MMP) and tissue inhibitors of metalloproteinase-1 (TIMP-1). Results show that (i) both mRNAs express at a relatively high level in the chorion laeve trophoblast cells and the adjacent decidual cells of fetal membrane; (ii) the most abundant expression of the two mRNAs was found in the extravillous trophoblast between Rohrs and Nitabuch striae of basal plate, trophoblast shell and gland cells of the decidua; (iii) isolated or small groups of cytotrophoblast cells in the chorionic villi and in the cells lining arterioles in decidua and stem villi also expressed both MT-1-MMP and TIMP-1 at defferent extents. The data suggest that the coordinated expression of the MT-MMP and its inhibitor TIMP in defferent cells of the placental tissue may play an essential role in trophoblast invasion and angiogenesis