A method was developed to determine the concentration of methyl tert-butyl ether(MTBE) in gasoline, diesel and heating oil by gas chromatography(GC) with mass spectrometry(GC-MS) or flame ionization detection(FID). Th...A method was developed to determine the concentration of methyl tert-butyl ether(MTBE) in gasoline, diesel and heating oil by gas chromatography(GC) with mass spectrometry(GC-MS) or flame ionization detection(FID). The diluted gasoline was directly injected into the GC, and the complete separation of MTBE from co-eluting hydrocarbons was not required. GC/MS or GC/FID method can be used to analyze MTBE in different concentration range and have good consistency.展开更多
The toxicity of methyl tert-butyl ether （MTBE） to Chlorella ellipsoidea and Aphanizomenon flos-aquae was tested and assessed for a 15-d incubation with concentrations of MTBE from high （2.00×10^4 mg/L） to low...The toxicity of methyl tert-butyl ether （MTBE） to Chlorella ellipsoidea and Aphanizomenon flos-aquae was tested and assessed for a 15-d incubation with concentrations of MTBE from high （2.00×10^4 mg/L） to low （2 mg/L）. The results showed that the toxicity was low when the concentration of MTBE was in the range 1.00× 10^4-2.00× 10^4 mg/L （the greatest inhibition of growth-rate was 70%-71%, occurred during the day 1-5）. Low concentrations （2-500 rag/L） stimulated algal growth up to the greatest effect of 85%-200% when the concentration of MTBE was 50-100 mg/L during day 3-5. The toxicity of MTBE （72-120 h EC50） was 6.65×10^3-9.58×10^3 mg/L for C. ellipsoidea and that is 1.14× 10^4-2.00× 10^4 mg/L for A. floc-aquae. We found that the toxicity and ecological risk of MTBE for the algal community structure were low and the toxicity was influenced by the duration time of the test.展开更多
The contamination of methyl ten-butyl ether （MTBE） in underground waters has become a widely concerned problem all over the world. In this study, a novel dosed culture system with oxygen supplied by H2O2 was introdu...The contamination of methyl ten-butyl ether （MTBE） in underground waters has become a widely concerned problem all over the world. In this study, a novel dosed culture system with oxygen supplied by H2O2 was introduced for MTBE aerobic biodegradation. After 7 d, almost all MTBE was degraded by a pure culture, a member of β-Proteobacteria named as PMI, in a closed system with oxygen supply, while only 40% MTBE was degraded in one without oxygen supply. Dissolved oxygen （DO） levels of the broth in closed systems respectively with and without H2O2 were about 5-6 and 4 mg/L. Higher DO may improve the activity of monooxygemase, which is the key enzyme of metabolic pathway from MTBE to tert-butyl alcohol and finally to CO2, and may result in the increase of the degrading activity of PM1 cell. The purge and trap GC-MS result of the broth in closed systems showed that tea-butyl alcohol, isopronol and acetone were the main intermediate products.展开更多
文摘A method was developed to determine the concentration of methyl tert-butyl ether(MTBE) in gasoline, diesel and heating oil by gas chromatography(GC) with mass spectrometry(GC-MS) or flame ionization detection(FID). The diluted gasoline was directly injected into the GC, and the complete separation of MTBE from co-eluting hydrocarbons was not required. GC/MS or GC/FID method can be used to analyze MTBE in different concentration range and have good consistency.
基金supported by the National Natural Science Foundation of China (No. 20476099)
文摘The toxicity of methyl tert-butyl ether （MTBE） to Chlorella ellipsoidea and Aphanizomenon flos-aquae was tested and assessed for a 15-d incubation with concentrations of MTBE from high （2.00×10^4 mg/L） to low （2 mg/L）. The results showed that the toxicity was low when the concentration of MTBE was in the range 1.00× 10^4-2.00× 10^4 mg/L （the greatest inhibition of growth-rate was 70%-71%, occurred during the day 1-5）. Low concentrations （2-500 rag/L） stimulated algal growth up to the greatest effect of 85%-200% when the concentration of MTBE was 50-100 mg/L during day 3-5. The toxicity of MTBE （72-120 h EC50） was 6.65×10^3-9.58×10^3 mg/L for C. ellipsoidea and that is 1.14× 10^4-2.00× 10^4 mg/L for A. floc-aquae. We found that the toxicity and ecological risk of MTBE for the algal community structure were low and the toxicity was influenced by the duration time of the test.
基金Project supported by the National Natural Science Foundation of China (No. 20476099) Zhejiang Provincial Natural Science Foundation (No. Y504272).
文摘The contamination of methyl ten-butyl ether （MTBE） in underground waters has become a widely concerned problem all over the world. In this study, a novel dosed culture system with oxygen supplied by H2O2 was introduced for MTBE aerobic biodegradation. After 7 d, almost all MTBE was degraded by a pure culture, a member of β-Proteobacteria named as PMI, in a closed system with oxygen supply, while only 40% MTBE was degraded in one without oxygen supply. Dissolved oxygen （DO） levels of the broth in closed systems respectively with and without H2O2 were about 5-6 and 4 mg/L. Higher DO may improve the activity of monooxygemase, which is the key enzyme of metabolic pathway from MTBE to tert-butyl alcohol and finally to CO2, and may result in the increase of the degrading activity of PM1 cell. The purge and trap GC-MS result of the broth in closed systems showed that tea-butyl alcohol, isopronol and acetone were the main intermediate products.
