The mitochondial control regions from 13 striped dolphins (Stenella coeruleualba), seven from the southern Taiwan Straits (Dongshan waters) and the other six from the southern East China Sea (Pingtan waters), were amp...The mitochondial control regions from 13 striped dolphins (Stenella coeruleualba), seven from the southern Taiwan Straits (Dongshan waters) and the other six from the southern East China Sea (Pingtan waters), were amplified by using polymerase chain reaction (PCR) technique. The complete nucleotide composition of this region, ranged from 914 to 917 base pairs (bp), were determined by an ABI 310 DNA automated sequencing system to address the sequence variability. 42 variable sites, including 36 transitions, three transversions, and three indels (insertion/deletion) were found. Of the variable sites, 26 were parsimonious informative sites. Totally 11 haplotypes were identified. Of the haplotypes, six were found exclusively in Dongshan waters and the rest five only in Pingtan waters. No common haplotype was shared, and two fixed site differences were detected between haplotypes from two waters. The haplotype diversty and nucleotide diversity were high for both waters, which were 1.45%±0.79% and 0.95 in Dongshan waters, and 1.06%±0.51% and 0.93 in Pingtan waters respectively. A phylogenetic tree was reconstructed by using neighbor-joining (NJ) method in MEGA (Molecular Evolutionary Genetic Analysis) package. In the tree, haplotypes from the Pingtan waters constituted a monophyletic clade, whereas haplotypes from the Dongshan waters did not. Analysis of molecular variance, which was conducted by computer software AMOVA, showed an significant population differentiation and genetic structure (Φ st-=0.35, P<0.01)between Dongshan waters and Pingtan waters. The haplotype diversity and nucleotide diversity of striped dolphins were higher than those found in the sympatric finless porpoise (Neophocaena phocaenoides) populations, and also higher than those of other cetacean species reported previously in other waters, including some species having high level of genetic diversity, e.g. harbor porpoises (Phocoena phocoena), humpback whales (Megaptera novaeangliae) and bottlenose dolphins (Tursiops spp.) etc. This suggested that the striped dolphins had a relatively higher genetic diversity.展开更多
基金国家自然科学基金项目 (No .3980 0 0 14 No .30 0 70 116 )国家自然科学基金重大项目 (No .3989940 0 )~~
文摘The mitochondial control regions from 13 striped dolphins (Stenella coeruleualba), seven from the southern Taiwan Straits (Dongshan waters) and the other six from the southern East China Sea (Pingtan waters), were amplified by using polymerase chain reaction (PCR) technique. The complete nucleotide composition of this region, ranged from 914 to 917 base pairs (bp), were determined by an ABI 310 DNA automated sequencing system to address the sequence variability. 42 variable sites, including 36 transitions, three transversions, and three indels (insertion/deletion) were found. Of the variable sites, 26 were parsimonious informative sites. Totally 11 haplotypes were identified. Of the haplotypes, six were found exclusively in Dongshan waters and the rest five only in Pingtan waters. No common haplotype was shared, and two fixed site differences were detected between haplotypes from two waters. The haplotype diversty and nucleotide diversity were high for both waters, which were 1.45%±0.79% and 0.95 in Dongshan waters, and 1.06%±0.51% and 0.93 in Pingtan waters respectively. A phylogenetic tree was reconstructed by using neighbor-joining (NJ) method in MEGA (Molecular Evolutionary Genetic Analysis) package. In the tree, haplotypes from the Pingtan waters constituted a monophyletic clade, whereas haplotypes from the Dongshan waters did not. Analysis of molecular variance, which was conducted by computer software AMOVA, showed an significant population differentiation and genetic structure (Φ st-=0.35, P<0.01)between Dongshan waters and Pingtan waters. The haplotype diversity and nucleotide diversity of striped dolphins were higher than those found in the sympatric finless porpoise (Neophocaena phocaenoides) populations, and also higher than those of other cetacean species reported previously in other waters, including some species having high level of genetic diversity, e.g. harbor porpoises (Phocoena phocoena), humpback whales (Megaptera novaeangliae) and bottlenose dolphins (Tursiops spp.) etc. This suggested that the striped dolphins had a relatively higher genetic diversity.
基金This work was supported by Shanghai Leading Academic Discipline Project (No.Y1101) and Grant from Key Academic Discipline of Aquaculture of Shanghai Fisheries University (No. 04SC11).
