Magnaporthe oryzae MTP1 gene encodes a type Ⅲ transmembrane protein involved in conidiation and conidial germination

In this study the MTP1 gene, encoding a type Ⅲ integral transmembrane protein, was isolated from the rice blast fungus Magnaporthe oryzae. The Mtp1 protein is 520 amino acids long and is comparable to the Ytp1 protein of Saccharomyces cerevisiae with 46% sequence similarity. Prediction programs and MTP1-GFP (green fluorescent protein) fusion expression results indicate that Mtp1 is a protein located at several membranes in the cytoplasm. The functions of the MTP1 gene in the growth and development of the fungus were studied using an MTP1 gene knockout mutant. The MTP1 gene was primarily ex-pressed at the hyphal and conidial stages and is necessary for conidiation and conidial germination, but is not required for patho-genicity. The Δmtp1 mutant grew more efficiently than the wild type strain on non-fermentable carbon sources, implying that the MTP1 gene has a unique role in respiratory growth and carbon source use.

芸薹属AC基因组MTP1基因的生物信息学分析

以拟南芥(Arabidopsis thaliana)AtMTP1基因序列为参考,利用白菜(Brassica rapa)、甘蓝(Brassica oleracea)、甘蓝型油菜(Brassica napus)全基因组数据对MTP1基因进行全基因组水平鉴定,通过Blastn比对和保守结构域分析,在芸薹属AC基因组中共鉴定到8个MTP1基因,其中白菜1个、甘蓝3个、甘蓝型油菜4个(A亚基因组3个、C亚基因组1个)。生物信息学分析结果表明,在蛋白质理化性质、二级结构、跨膜结构域、磷酸化位点等方面,3个物种中的MTP1基因间各有差异,并使用甘蓝型油菜转录组数据比较分析4个BnMTP1基因在不同组织部位的表达模式。