恶性淋巴瘤中MTS1-p^(16)基因的缺失

目的 研究ＭＴＳ１ｐ１６ 基因在恶性淋巴瘤中的变化。方法 采用多重ＰＣＲ 检测３４ 例非何杰金淋巴瘤（ＮＨＬ） 和１５ 例健康人体外周血ＤＮＡ 的ｐ１６ｇｅｎｅ Ｅｘｏｎ２ 纯合子缺失。结果 ３４ 例ＮＨＬ中有４ 例有ｐ１６ｇｅｎｅ Ｅｘｏｎ２ 纯合子缺失。结论 ＭＴＳ１Ｐ１６ｇｅｎｅ 变异可能在非何杰金淋巴瘤的发生发展中起作用。

鼻咽癌患者P^(16)/MTS_1抑癌基因失活研究

目的：以原发性鼻咽癌（NPC）患者为研究对象，检测鼻咽癌组织中P16／MTSI抑癌基因失活情况；研究鼻咽癌发生发展规律。方法：采用PCR、多重PCR、限制性内切酶解一PCR方法对34例鼻咽癌患者和34例非肿瘤（腺样体增殖及慢性炎症）患者，进行P基因纯会缺失和异常甲基化检测。结果：34例NPC患者纯缺失率为8．82％（3／34），异常甲基化率为38．24％（13／34），34例非肿瘤患者纯缺失未检出（0／34），异常甲基化率为8．82％（3／34）。结论：本研究鼻咽癌患者P16／MTSI抑癌基因总失活率为47．06％（16／34），表明P16基因失活在鼻咽癌患者中是较为常见的基因变化。鼻咽癌患者中甲基化异常是P16基因失活的另一重要途径，值得引起重视。

FREQUENT DELETION OF MTS1/p16 GENE AND CORRELATION WITH CLINICOPATHOLOGICAL PARAMETERS IN ENDOMETRIAL CARCINOMA

Objective: To investigate the possible relationship between deletion of MTS/p16 gene and progression of endometrial carcinoma Methods: Forty six primary endometrial carcinoma, 7 tumor adjacent endometrial tissue, 10 normal endometrial tissue specimen and 5 xenografts from patients with endometrial carcinoma were examined for homozygous deletion of MTS/p16 gene by polymerase chain reaction based analysis Results: Of 46 endometrial cancer specimens, 9 showed homozygous deletion, no deletion was detected in the tumor adjacent and normal endometial tissues Nor was it detected in well differentiated endometrial carcinoma and all xenografts Conclusions: Deletion of MTS1/p16 gene might contribute to the progression of endometrial carcinoma and could be served as indicator for predicting prognosis

急性淋巴细胞白血病P^(16)基因纯合子缺失的研究

目的 :为了解 P1 6 gene变异与急性淋巴细胞白血病 (AL L)发生发展的关系。方法 :采用差别 PCR分析了 30例 AL L骨髓 DNA和 15例非恶性血液病骨髓 DNA P1 6 gene纯合缺失。结果 :30例 AL L 患者骨髓 DNA有 10例 P1 6 gene Exon2纯合缺失 ,纯合缺失率为 33.3%。结论 :P1 6 gene变异可能是 AL L 发生发展中的重要因素。

Expression,deleton and mnutation of ρ16 gene in human gastric cancer

AIM To investigate the relationship between the expression of p16 gene and the gastric carcinogenesis,depth of invasion and lymph node metastases, and to evaluate the deletion and mutation of exon 2 in p16 gene in gastric carcinoma.METHODS The expression of P16 protein was examined by streptavidin-peroxidase conjugated method (S-P); the deletion and mutation of p16 gene were respectively examined by polymerase chain reaction (PCR) and polymerase chain reaction single-strand conformation polymorphism analysis (PCR-SSCP) in gastric carcinoma.RESULTS Expression of P16 protein was detected in 96.25% (77/80) of the normal gastric mucosa, in 92.00% (45/50) of the dysplastic gastric mucosa and in 47.54% (58/122) of the gastric carcinoma. The positive rate of P16 protein expression in gastric carcinoma was significantly lower than that in normal gastric mucosa and dysplastic gastric mucosa (P＜0.05). The positive rate of P16 protein expression in mucoid carcinoma 10.00% (1/ 10) was significantly lower than that in poorly differentiated carcinoma 51.22% ( 21/ 41 ),undifferentiated carcinoma 57.69% (15/26) and signet ring cell carcinoma 62.50% (10/ 16) (P＜0.05). The positive rate of p16 protein in 30 cases paired primary and lymph node metastatic gastric carcinoma: There was 46.67% (14/30) in primary gastric carcinoma, 16.67% (5/30) in lymph node metastatic gastric carcinoma. The positive rate of lymph node metastatic carcinoma was significantly lower than that of primary carcinoma (P＜0.05). There was of p16 gene mutation in exon 2, but 5 cases displayed deletion of p16 gene in exon 2 in the 25 primary gastric carcinomas.CONCLUSIONS The expression loss of P16 protein related to the gastric carcinogenesis, gastric carcinoma histopathological subtypes and lymph metastasis. The mutation of p16 gene in exon 2 may not be involved in gastric carcinogenesis. But the deletion of p16 gene in exon 2 may be involved in gastric carcinogenesis.

非要素经肠营养剂对进展期胃癌围手术期的营养支持

进展期胃癌病人，多伴有不同程度的营养不良和免疫功能受损。本实验旨在应用非要素经肠营养剂改善此类病人的营养状况和免疫功能，以提高其手术及术后放、化疗的耐受力。用经肠营养剂经口服或管饲对进展期胃癌病人围手术期进行营养支持，并设等热等氮的肠外营养组进行对照，观察血浆蛋白等营养指标及免疫球蛋白等免疫指标。结果表明，营养支持后，实验组病人营养指标的改善与对照组相近，但ＩｇＧ、ＩｇＡ、ＩｇＭ 等多项免疫指标明显优于对照组。说明用非要素经肠营养剂对进展期胃癌病人在围手术期进行营养支持，其疗效明显优于等热等氮的肠外营养支持。