MTUS1 （microtubule-associated tumor suppressor 1） has been identified that can function as a tumor sup- pressor gene in many malignant tumors. However, the function and mechanisms underlying the regulation of MTUS1 ...MTUS1 （microtubule-associated tumor suppressor 1） has been identified that can function as a tumor sup- pressor gene in many malignant tumors. However, the function and mechanisms underlying the regulation of MTUS1 are unclear. In the present study, we reported that miR-19a and miR-19b （miR-19a/b） promote prolifer- ation and migration of lung cancer cells by targeting MTUS1. First, MTUS1 was proved to function as a tumor suppressor in lung cancer and was linked to cell prolif- eration and migration promotion. Second, an inverse correlation between miR-19a/b expression and MTUS1 mRNA/protein expression was noted in human lung cancer tissues. Third, MTUS1 was appraised as a direct target of miR-19a/b by bioinformatics analysis. Fourth, direct MTUS1 regulation by miR-19a/b in lung cancer cells was experimentally affirmed by cell transfection assay and luciferase reporter assay. Finally, miR-19a/b were shown to cooperatively repress MTUS1 expression and synergistically regulate MTUS1 expression to pro- mote lung cancer cell proliferation and migration. In conclusion, our findings have provided the first cluesregarding the roles of miR-19a/b, which appear to func- tion as oncomirs in lung cancer by downregulating MTUSI.展开更多
文摘MTUS1 （microtubule-associated tumor suppressor 1） has been identified that can function as a tumor sup- pressor gene in many malignant tumors. However, the function and mechanisms underlying the regulation of MTUS1 are unclear. In the present study, we reported that miR-19a and miR-19b （miR-19a/b） promote prolifer- ation and migration of lung cancer cells by targeting MTUS1. First, MTUS1 was proved to function as a tumor suppressor in lung cancer and was linked to cell prolif- eration and migration promotion. Second, an inverse correlation between miR-19a/b expression and MTUS1 mRNA/protein expression was noted in human lung cancer tissues. Third, MTUS1 was appraised as a direct target of miR-19a/b by bioinformatics analysis. Fourth, direct MTUS1 regulation by miR-19a/b in lung cancer cells was experimentally affirmed by cell transfection assay and luciferase reporter assay. Finally, miR-19a/b were shown to cooperatively repress MTUS1 expression and synergistically regulate MTUS1 expression to pro- mote lung cancer cell proliferation and migration. In conclusion, our findings have provided the first cluesregarding the roles of miR-19a/b, which appear to func- tion as oncomirs in lung cancer by downregulating MTUSI.
