The potential efficacy and clinical feasibility of gene therapy for liver cancer were tested through therecombinant adenovirus-mediated (Ad-multigenes ) co-transfer of human wild-type p53, B7-l co-stimulation(CD8o) an...The potential efficacy and clinical feasibility of gene therapy for liver cancer were tested through therecombinant adenovirus-mediated (Ad-multigenes ) co-transfer of human wild-type p53, B7-l co-stimulation(CD8o) and granulocyte-macrophage colony-stimulating factor (GM-CSF) genes into human hepatocellular carcinoma cell lines. The treated cells underwent apoptosis with specific DNA fragmentation and became more sensitiveto cisplatin, a chemotherapeutic drug. Their growth was partly inhibited. Efficient proliferation and generation ofCTLs and cytokine production were induced in mixed lymphocytes through tumor cell reaction (MLTR) using peripheral blood T lymphocytes from donors as effector cells and Ad-multigenes or Ad-p53-transfected human hepatocellular carcinoma cells (HepG2 or BEL7402) as stimulator cells. Ad-multigenes-transfected rat carcinosarcomaWalker 256 cells were inoculated subcutaneously into normal rats. Fourteen days later, the activity of spleen cellsin rats inoculated with Ad-multigenes-transduced Walker 256 cells was higher than that in Ad-p53-transducedones. These findings suggest that adenovirus-mediated multigenes p53, B7-1 and GM-CSF can induce apoptosis ofliver cancer cells and initiate a potent antitumor immune response against them.展开更多
AIM: Glutathione S-transferase mu 1 (GSTM1) and theta 1 (GSTT1) genes are involved in the metabolism of a wide range of carcinogens, but deletions of the genes are commonly found in the population. The present study w...AIM: Glutathione S-transferase mu 1 (GSTM1) and theta 1 (GSTT1) genes are involved in the metabolism of a wide range of carcinogens, but deletions of the genes are commonly found in the population. The present study was undertaken to evaluate the association between GSTM1 and GSTT1 gene polymorphisms and hepatocellular carcinoma (HCC) risk. METHODS: The genetic polymorphisms were studied at an aflatoxin highly contaminated region in Guangxi, China. Pdymerase chain reaction (PCR) technique was used to detect the presence or absence of the GSTM1 and GSTT1 genes in blood samples. The case group was composed of 181 patients of HCC identified by the pathologists and the control group was composed of 360 adults without any tumor. RESULTS: The frequencies of GSTM1 and GSTT1 null genotypes in the control were 47.8% and 42.7%, while those in the HCC group were 64.6% and 59.7%, respectively. The differences between HCC group and control group were very significant (P<0.01). GSTM1 and GSTT1 combined null genotypes in HCC group and control group were 38.2% and 18.5% respectively, and the difference was significant (P<0.05). CONCLUSION: The GSTM1 and GSTT1 null genotypes are associated with an increased risk of HCC in a special geographic environment. Combination of the two null genotypes in an individual is substantially increased twice the risk of HCC.展开更多
Cultured meat produced through in vitro cell culture technology is regarded as a technical revolution.In this study,three-dimensional(3D)bioprinting technology was used to mimic the growth environment in vivo and cons...Cultured meat produced through in vitro cell culture technology is regarded as a technical revolution.In this study,three-dimensional(3D)bioprinting technology was used to mimic the growth environment in vivo and construct a 3D culture system in vilro.Hydroge1 bioinks,namely,sodium alginate-gelatin and gelatin-methacrylate(GelMA)-silk fbroin,produced using two different curing processes were blended,and their rheological properties,mechanical properties,and biocompatibilities were compared.The 4%GelMA-20%silk fibroin hydroge1(GS2)demonstrated good performance and was hybridized with porcine skeleta1 muscle satellite cells for 3D printing to construct network structures of size 15 mm×15 mm and porosity 1000 um in 4-,6-,and 8-1ayer struchures.After 16 days of culture,4-and 6-1ayer grid structures formed compact muscle fbers organized by multinucleated myotubes.These results suggested that 3D bioprinting and GeIMA-silk fbroin hydrogels have great potential in fabricating porcine skeleta1 muscle tissue for use as cultured meat.展开更多
On the bases of the properties of abstract hierarchical structure model and the concrete structure of the mode1 system. which is convenient to solve practical problems, a visual interactive hierarchical coordination m...On the bases of the properties of abstract hierarchical structure model and the concrete structure of the mode1 system. which is convenient to solve practical problems, a visual interactive hierarchical coordination method has been proposed. In this paper, a compensation adjustment sub-mode1 for hydropower stations, an optimal operation sub-model for hydro-thermal power systems, and an aggregation model based on the aspiration level theory are built, and these models can be solved with decision support algorithm. The set of objectives and its structure could be made by the decision-maker in visua1 software, which could be decided by AHP. Finally, the application results show that this methodology is feasible, however, the software (DSS) needs further improvement.展开更多
文摘The potential efficacy and clinical feasibility of gene therapy for liver cancer were tested through therecombinant adenovirus-mediated (Ad-multigenes ) co-transfer of human wild-type p53, B7-l co-stimulation(CD8o) and granulocyte-macrophage colony-stimulating factor (GM-CSF) genes into human hepatocellular carcinoma cell lines. The treated cells underwent apoptosis with specific DNA fragmentation and became more sensitiveto cisplatin, a chemotherapeutic drug. Their growth was partly inhibited. Efficient proliferation and generation ofCTLs and cytokine production were induced in mixed lymphocytes through tumor cell reaction (MLTR) using peripheral blood T lymphocytes from donors as effector cells and Ad-multigenes or Ad-p53-transfected human hepatocellular carcinoma cells (HepG2 or BEL7402) as stimulator cells. Ad-multigenes-transfected rat carcinosarcomaWalker 256 cells were inoculated subcutaneously into normal rats. Fourteen days later, the activity of spleen cellsin rats inoculated with Ad-multigenes-transduced Walker 256 cells was higher than that in Ad-p53-transducedones. These findings suggest that adenovirus-mediated multigenes p53, B7-1 and GM-CSF can induce apoptosis ofliver cancer cells and initiate a potent antitumor immune response against them.
基金Supported by The Natural Scientific Foundation of China No. 39860032by the Education Department of Guangxi Zhuang Autonomous Region No. 98-2-8
文摘AIM: Glutathione S-transferase mu 1 (GSTM1) and theta 1 (GSTT1) genes are involved in the metabolism of a wide range of carcinogens, but deletions of the genes are commonly found in the population. The present study was undertaken to evaluate the association between GSTM1 and GSTT1 gene polymorphisms and hepatocellular carcinoma (HCC) risk. METHODS: The genetic polymorphisms were studied at an aflatoxin highly contaminated region in Guangxi, China. Pdymerase chain reaction (PCR) technique was used to detect the presence or absence of the GSTM1 and GSTT1 genes in blood samples. The case group was composed of 181 patients of HCC identified by the pathologists and the control group was composed of 360 adults without any tumor. RESULTS: The frequencies of GSTM1 and GSTT1 null genotypes in the control were 47.8% and 42.7%, while those in the HCC group were 64.6% and 59.7%, respectively. The differences between HCC group and control group were very significant (P<0.01). GSTM1 and GSTT1 combined null genotypes in HCC group and control group were 38.2% and 18.5% respectively, and the difference was significant (P<0.05). CONCLUSION: The GSTM1 and GSTT1 null genotypes are associated with an increased risk of HCC in a special geographic environment. Combination of the two null genotypes in an individual is substantially increased twice the risk of HCC.
文摘Cultured meat produced through in vitro cell culture technology is regarded as a technical revolution.In this study,three-dimensional(3D)bioprinting technology was used to mimic the growth environment in vivo and construct a 3D culture system in vilro.Hydroge1 bioinks,namely,sodium alginate-gelatin and gelatin-methacrylate(GelMA)-silk fbroin,produced using two different curing processes were blended,and their rheological properties,mechanical properties,and biocompatibilities were compared.The 4%GelMA-20%silk fibroin hydroge1(GS2)demonstrated good performance and was hybridized with porcine skeleta1 muscle satellite cells for 3D printing to construct network structures of size 15 mm×15 mm and porosity 1000 um in 4-,6-,and 8-1ayer struchures.After 16 days of culture,4-and 6-1ayer grid structures formed compact muscle fbers organized by multinucleated myotubes.These results suggested that 3D bioprinting and GeIMA-silk fbroin hydrogels have great potential in fabricating porcine skeleta1 muscle tissue for use as cultured meat.
文摘On the bases of the properties of abstract hierarchical structure model and the concrete structure of the mode1 system. which is convenient to solve practical problems, a visual interactive hierarchical coordination method has been proposed. In this paper, a compensation adjustment sub-mode1 for hydropower stations, an optimal operation sub-model for hydro-thermal power systems, and an aggregation model based on the aspiration level theory are built, and these models can be solved with decision support algorithm. The set of objectives and its structure could be made by the decision-maker in visua1 software, which could be decided by AHP. Finally, the application results show that this methodology is feasible, however, the software (DSS) needs further improvement.
