[Objectives] This study was conducted to establish a method for determination of chlorogenic acid in Mussaenda pubescens Ait.f.[Methods]HPLC used SHISEIDO C_(18) MG Ⅱ column( 5 μm,4.6 mml.D.× 250 mm) as chromat...[Objectives] This study was conducted to establish a method for determination of chlorogenic acid in Mussaenda pubescens Ait.f.[Methods]HPLC used SHISEIDO C_(18) MG Ⅱ column( 5 μm,4.6 mml.D.× 250 mm) as chromatographic column and acetonitrile-0.4% phosphoric acid solution( 9∶91) as mobile phase.The separation was performed at a flow rate of 1.0 ml/min and a column temperature of 30 ℃,and the detection wavelength was set at 327 nm.[Results]Chlorogenic acid had a good linear relation in the range of 0.254 7-2.547 5 μg( R2= 0.999 9).The recovery rate was 97.8%,RSD = 1.82%( n = 9).[Conclusions]The content of chlorogenic acid in M.pubescens was determined by ultrasonic extraction and HPLC.The method was simple,stable and reliable and could be used for the quality control of M.pubescens.展开更多
A new bis-iridoid glucoside, incaside 1 was isolated along with known iridoid, 6-0- veratroylcatalposide from the stem-bark of Mussaenda incana. The structure of the new iridoid was established mainly on the basis of ...A new bis-iridoid glucoside, incaside 1 was isolated along with known iridoid, 6-0- veratroylcatalposide from the stem-bark of Mussaenda incana. The structure of the new iridoid was established mainly on the basis of the spectroscopic （including 2D NMR） and chemical studies of its octaacetate 1a.展开更多
A cyclolanostene-type triterpene and two saponins derived thereform, namely MP-C,D and F,were isolated from the acid-hydrolyzate of the mixture of saponins of Mussaenda pubesecus.Their structures were elucidated on th...A cyclolanostene-type triterpene and two saponins derived thereform, namely MP-C,D and F,were isolated from the acid-hydrolyzate of the mixture of saponins of Mussaenda pubesecus.Their structures were elucidated on the basis of chemical and UV,IR,~1H,^(13)C NMR and MS spectral evidence.The two new saponins MP-D and F were named Mussaendoside A and C,respectively.展开更多
报道了在云南发现的玉叶金花属Mussaenda一新种——狭瓣玉叶金花M. lancipetala X. F. Deng & D. X. Zhang。狭瓣玉叶金花的性系统为柱头缩入式雌雄异位; 花冠裂片卵状披针形且具有细长的长尖头, 花冠管外被有粉末状的毛被, 可以很...报道了在云南发现的玉叶金花属Mussaenda一新种——狭瓣玉叶金花M. lancipetala X. F. Deng & D. X. Zhang。狭瓣玉叶金花的性系统为柱头缩入式雌雄异位; 花冠裂片卵状披针形且具有细长的长尖头, 花冠管外被有粉末状的毛被, 可以很容易与玉叶金花属其他种类相区别。展开更多
Reproductive isolation is a fundamental require- ment for speciation and includes several sequential stages. Few studies have determined the relative contributions of pre- and post-zygotic reproductive isolation in pl...Reproductive isolation is a fundamental require- ment for speciation and includes several sequential stages. Few studies have determined the relative contributions of pre- and post-zygotic reproductive isolation in plants, especially between relative species with clear differentiation in flower form. To investigate the mechanisms responsible for repro- ductive isolation in sympatric A4ussaenda pubescens var. alba and Mussaenda shikokiana （Rubiaceae） in Guangxi Province, China, we made observations of flowering phenology, patterns of insect visitation, and conducted pollination experiments, including artificial hybridization. The two species had over- lapping flowering times and were pollinated by overlapping pollinators; however, their relative importance differed significantly with M. pubescens visited more commonly by bees and M. shikokiana more frequently by butterflies. Using vegetative and floral characters and molecular evidence based on nuclear ribosomal internal and external transcribed spacer regions we detected seven naturally occurring hybrids among a sample of approximately 125 individuals. Hybrids were characterized by morphologies that most closely resembled their maternal parents based on chloroplast evidence. Studies of artificially synthesized and natural hybrids demonstrated that hybrid seed had very low germination rates and naturally occurring hybrids exhibited pollen sterility. Post-zygotic reproductive isolating mechanisms play a primary role in limiting gene exchange between co-occurring species and maintaining species integrity in areas of sympatry.展开更多
Reproductive isolation defines the biological species concept and plays a key role in the formation and maintenance of species. The relative contributions of different isolating stages has been suggested to be closely...Reproductive isolation defines the biological species concept and plays a key role in the formation and maintenance of species. The relative contributions of different isolating stages has been suggested to be closely associated with phylogenetic relatedness. Few studies have focused on the relative contributions of pre- versus post-zygotic mechanisms, and even fewer have been conducted under strict phylogenetic frameworks. Pre-and post-zygotic reproductive isolation stages have been investigated in the sister species Mussaenda kwangtungensis and M. pubescens var. alba. The two species have partly overlapping distribu-tion ranges and flowering times, while the principal pollinators differed strikingly for them, demonstrating strong pre-zygotic isolations. Natural hybrids were detected by simple sequence repeat markers and their maternal parents were identified based on chloroplast gene sequen-ces. Five out of 81 individuals were suggested to be hybrids that fall into the categories F2, BC1, and BC2 by the NewHybrids analysis. Interspecific crossings resulted in significantly reduced fruit set and seed germination rates. Phylogenetic analysis revealed short Kimura-2-parameter distance between M. kwangtungensis and M. pubescens var. alba. These findings strongly supported the hypothesis that for species with a closer phylogenetic relationship, pre-zygotic isolation plays an important part in limiting gene exchange in sympatric areas.展开更多
目的：建立玉叶金花总三萜类成分含量的测定方法。方法：以熊果酸作对照品,采用L9（34）正交设计优选显色条件,并测定玉叶金花药材中总三萜类成分的含量。结果：最佳显色条件为：5%香草醛-冰醋酸溶液0.6 m L,高氯酸0.6 m L,70℃,反应15 ...目的：建立玉叶金花总三萜类成分含量的测定方法。方法：以熊果酸作对照品,采用L9（34）正交设计优选显色条件,并测定玉叶金花药材中总三萜类成分的含量。结果：最佳显色条件为：5%香草醛-冰醋酸溶液0.6 m L,高氯酸0.6 m L,70℃,反应15 min;该方法在0.021-0.16 mg含量范围内线性关系良好（R=0.9995）,当显色反应完全后对照品和供试品液在70 min内稳定性良好,精密度高（RSD=2.00%,n=5）,平均回收率为100.08%（RSD=1.18%,n=6）。结论：香草醛比色法适合玉叶金花总三萜类成分的含量测定,可用于其药材的质量控制。展开更多
基金Supported by Guangzi Post-doctor Innovative Practice Base Open Project(2017002A)
文摘[Objectives] This study was conducted to establish a method for determination of chlorogenic acid in Mussaenda pubescens Ait.f.[Methods]HPLC used SHISEIDO C_(18) MG Ⅱ column( 5 μm,4.6 mml.D.× 250 mm) as chromatographic column and acetonitrile-0.4% phosphoric acid solution( 9∶91) as mobile phase.The separation was performed at a flow rate of 1.0 ml/min and a column temperature of 30 ℃,and the detection wavelength was set at 327 nm.[Results]Chlorogenic acid had a good linear relation in the range of 0.254 7-2.547 5 μg( R2= 0.999 9).The recovery rate was 97.8%,RSD = 1.82%( n = 9).[Conclusions]The content of chlorogenic acid in M.pubescens was determined by ultrasonic extraction and HPLC.The method was simple,stable and reliable and could be used for the quality control of M.pubescens.
文摘A new bis-iridoid glucoside, incaside 1 was isolated along with known iridoid, 6-0- veratroylcatalposide from the stem-bark of Mussaenda incana. The structure of the new iridoid was established mainly on the basis of the spectroscopic （including 2D NMR） and chemical studies of its octaacetate 1a.
文摘A cyclolanostene-type triterpene and two saponins derived thereform, namely MP-C,D and F,were isolated from the acid-hydrolyzate of the mixture of saponins of Mussaenda pubesecus.Their structures were elucidated on the basis of chemical and UV,IR,~1H,^(13)C NMR and MS spectral evidence.The two new saponins MP-D and F were named Mussaendoside A and C,respectively.
基金supported by the Science Foundation of China,Grant No.30570314Chinese Academy of Sciences Knowledge Innovation Program,Grant No.KSCX2-YW-Z-027the Natural Science Foundation of Guangdong Province,Grant No.04002308.
文摘报道了在云南发现的玉叶金花属Mussaenda一新种——狭瓣玉叶金花M. lancipetala X. F. Deng & D. X. Zhang。狭瓣玉叶金花的性系统为柱头缩入式雌雄异位; 花冠裂片卵状披针形且具有细长的长尖头, 花冠管外被有粉末状的毛被, 可以很容易与玉叶金花属其他种类相区别。
基金supported by the National Natural Science Foundation of China (31170184, U1202261, 31000109)the China Scholarship Council (for C. S.’s study in Canada)
文摘Reproductive isolation is a fundamental require- ment for speciation and includes several sequential stages. Few studies have determined the relative contributions of pre- and post-zygotic reproductive isolation in plants, especially between relative species with clear differentiation in flower form. To investigate the mechanisms responsible for repro- ductive isolation in sympatric A4ussaenda pubescens var. alba and Mussaenda shikokiana （Rubiaceae） in Guangxi Province, China, we made observations of flowering phenology, patterns of insect visitation, and conducted pollination experiments, including artificial hybridization. The two species had over- lapping flowering times and were pollinated by overlapping pollinators; however, their relative importance differed significantly with M. pubescens visited more commonly by bees and M. shikokiana more frequently by butterflies. Using vegetative and floral characters and molecular evidence based on nuclear ribosomal internal and external transcribed spacer regions we detected seven naturally occurring hybrids among a sample of approximately 125 individuals. Hybrids were characterized by morphologies that most closely resembled their maternal parents based on chloroplast evidence. Studies of artificially synthesized and natural hybrids demonstrated that hybrid seed had very low germination rates and naturally occurring hybrids exhibited pollen sterility. Post-zygotic reproductive isolating mechanisms play a primary role in limiting gene exchange between co-occurring species and maintaining species integrity in areas of sympatry.
基金supported by the National Natural Science Foundation of China (31170184, U1202261, 31000109, 31370269)the International Foundation for Science (AD/22476)
文摘Reproductive isolation defines the biological species concept and plays a key role in the formation and maintenance of species. The relative contributions of different isolating stages has been suggested to be closely associated with phylogenetic relatedness. Few studies have focused on the relative contributions of pre- versus post-zygotic mechanisms, and even fewer have been conducted under strict phylogenetic frameworks. Pre-and post-zygotic reproductive isolation stages have been investigated in the sister species Mussaenda kwangtungensis and M. pubescens var. alba. The two species have partly overlapping distribu-tion ranges and flowering times, while the principal pollinators differed strikingly for them, demonstrating strong pre-zygotic isolations. Natural hybrids were detected by simple sequence repeat markers and their maternal parents were identified based on chloroplast gene sequen-ces. Five out of 81 individuals were suggested to be hybrids that fall into the categories F2, BC1, and BC2 by the NewHybrids analysis. Interspecific crossings resulted in significantly reduced fruit set and seed germination rates. Phylogenetic analysis revealed short Kimura-2-parameter distance between M. kwangtungensis and M. pubescens var. alba. These findings strongly supported the hypothesis that for species with a closer phylogenetic relationship, pre-zygotic isolation plays an important part in limiting gene exchange in sympatric areas.
文摘目的：建立玉叶金花总三萜类成分含量的测定方法。方法：以熊果酸作对照品,采用L9（34）正交设计优选显色条件,并测定玉叶金花药材中总三萜类成分的含量。结果：最佳显色条件为：5%香草醛-冰醋酸溶液0.6 m L,高氯酸0.6 m L,70℃,反应15 min;该方法在0.021-0.16 mg含量范围内线性关系良好（R=0.9995）,当显色反应完全后对照品和供试品液在70 min内稳定性良好,精密度高（RSD=2.00%,n=5）,平均回收率为100.08%（RSD=1.18%,n=6）。结论：香草醛比色法适合玉叶金花总三萜类成分的含量测定,可用于其药材的质量控制。
