Germplasm resources of three wood plant species enriched with nervonic acid

Nervonic acid(NA)is a very-long-chain monounsaturated fatty acid with pharmaceutical and nutraceutical functions that plays an important role in treating several neurological disorders.One major source of NA is plant seed oil.Here we report fatty acid profiles of seeds and germplasm diversity of six plant species,including three woody plants with high amounts of NA-enriched seed oil,Malania oleifera,Macaranga adenantha,and M.indica.M.oleifera had the largest seed(average 7.40 g single seed),highest oil content(58.71%),and highest NA level(42.22%).The germplasm diversity of M.oleifera is associated with its habitat but not elevation.Seeds of M.adenantha contained higher NA levels(28.41%)than M.indica(21.77%),but M.indica contained a significantly higher oil content(29.22%)and seed yield.M.adenantha germplasm varied among populations,with one population having seeds with high oil content(22.63%)and NA level(37.78%).Although M.indica grow naturally at a range of elevations,no significant differences were detected between M.indica populations.These results suggest that M.indica and M.oleifera have greater potential as a source of NA,which will contribute to constructing a germplasm resource nursery and establishing a selection and breeding program to improve the development of NA-enriched plants.

印度血桐与中平树基因组调查及SSR分子标记分析

印度血桐与中平树是大戟科血桐属植物,该属植物具有多种药用价值,被广泛应用于民间医学中许多疾病的治疗,这两种植物种子中含有的神经酸也引起了研究者的高度关注。为确定适合印度血桐与中平树的全基因组测序研究策略,该研究采用二代高通量测序技术,结合生物信息学的方法首次测定了印度血桐与中平树的基因组大小、杂合率、重复率等基因组信息并初步分析了两种材料的SSR序列特征。结果表明:(1)印度血桐与中平树的基因组大小分别为986.84和946.23 M。(2)印度血桐与中平树的杂合率分别为0.75%和0.65%,重复序列比例分别为73.02%和71.5%。(3)通过对2种材料基因组序列的SSR特征分析,在印度血桐中共鉴定了4499185个SSR,在中平树中共鉴定了4969098个SSR。该研究结果为印度血桐与中平树SSR分子标记的筛选、开发以及全基因组深度测序提供了理论指导。