Well-defined raspberry-like magnetic microbeads （RMMBs） as immunoassay solid carriers were pre- pared by chemicM covalent binding between Fe304 magnetic microspheres and SiO2 nanoparticles. These RMMBs were not as a...Well-defined raspberry-like magnetic microbeads （RMMBs） as immunoassay solid carriers were pre- pared by chemicM covalent binding between Fe304 magnetic microspheres and SiO2 nanoparticles. These RMMBs were not as agglomerative as nano-sized magnetie particles （〈 200 nm）, which was an advangtage for high efficient magnetic separation. When compared to Fe304@SiO2 core-shell magnetic microbeads （CMMBs） with smooth surface, RMMBs exhibited stronger capacity to bind biomolecules. Limit of blank （LOB） and limit of detection （LoD） of HBsAg detection using RMMBs as carriers via chemiluminiscence immunoassay （CLIA） were 0.472 and 1.022 μg/L, respectively, showing a notable improvement compared with CMMBs whose LoB and LoD were 1.017 and 1.988 μg/L, respectively. All these indicated a great potential of RMMBs in immunoassay application.展开更多
基金the National Natural Science Foundation of China(No.21075082)the National High Technology Research and Development Program(863) of China(No.2012AA020103)+3 种基金the Shanghai Nano Program(No.11nm0505600)the Shanghai Jiao Tong University Funding(No.YG2012ZD03)the 2012 Shanghai Jiao Tong University and University of Michigan Collaborative Research Projects(No.12X120010007)the Shanghai Municipal Education Commission Project(No.14ZZ023)
文摘Well-defined raspberry-like magnetic microbeads （RMMBs） as immunoassay solid carriers were pre- pared by chemicM covalent binding between Fe304 magnetic microspheres and SiO2 nanoparticles. These RMMBs were not as agglomerative as nano-sized magnetie particles （〈 200 nm）, which was an advangtage for high efficient magnetic separation. When compared to Fe304@SiO2 core-shell magnetic microbeads （CMMBs） with smooth surface, RMMBs exhibited stronger capacity to bind biomolecules. Limit of blank （LOB） and limit of detection （LoD） of HBsAg detection using RMMBs as carriers via chemiluminiscence immunoassay （CLIA） were 0.472 and 1.022 μg/L, respectively, showing a notable improvement compared with CMMBs whose LoB and LoD were 1.017 and 1.988 μg/L, respectively. All these indicated a great potential of RMMBs in immunoassay application.
