Magnetically modified palygorskite composites were synthesized with γ-Fe2O3 dispersing on the external surface of clay mineral. The magnetic clay was characterized with Fourier transform infrared, X-ray diffraction, ...Magnetically modified palygorskite composites were synthesized with γ-Fe2O3 dispersing on the external surface of clay mineral. The magnetic clay was characterized with Fourier transform infrared, X-ray diffraction, transmission electron microscopy, and vibrating sample magnetometer. Candida sp. 99–125 lipase was immobilized on magnetic palygorskite composites by physical adsorption with enzyme loading of41.5 mg·g-1support and enzyme activity of 2631.6 U·(g support)-1. The immobilized lipase exhibit better thermal and broader p H stability and excellent reusability compared with free lipase.展开更多
基金the National Basic Research Program of China(2009CB724700)the Foundation of Jiangsu Province of China for College Postgraduate Students in Innovation Engineering(CXZZ12_0440)
文摘Magnetically modified palygorskite composites were synthesized with γ-Fe2O3 dispersing on the external surface of clay mineral. The magnetic clay was characterized with Fourier transform infrared, X-ray diffraction, transmission electron microscopy, and vibrating sample magnetometer. Candida sp. 99–125 lipase was immobilized on magnetic palygorskite composites by physical adsorption with enzyme loading of41.5 mg·g-1support and enzyme activity of 2631.6 U·(g support)-1. The immobilized lipase exhibit better thermal and broader p H stability and excellent reusability compared with free lipase.