Objective: To explore the molecular mechanism of Magnoliae Flos and Xanthii Fructus herb pair for allergic rhinitis based on network pharmacology. Methods: From the Traditional Chinese Medicine Systems Pharmacology da...Objective: To explore the molecular mechanism of Magnoliae Flos and Xanthii Fructus herb pair for allergic rhinitis based on network pharmacology. Methods: From the Traditional Chinese Medicine Systems Pharmacology database, Uniprot database, and Gene Cards database, the relevant chemical constituents information, pharmacokinetic information and hub target of allergic rhinitis were obtained. The protein-protein interaction network was constructed by STRING online database, analyzed and showed by the Cytoscape software. The screened target information was analyzed by the Metascape database for Gene Ontology biological function and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis. Results: Main components of Magnoliae Flos and Xanthii Fructus herb pair, such as aloe-emodin, stigmasterol, beta-sitosterol and Yangambin, depend on the interaction of Nitric Oxide Synthase 3, Tumor Necrosis Factor, Caspase-3 and other functions involve G protein-coupled (amine) receptor activity, RNA polymerase II basic transcription factor binding, protease binding, heme binding, and integrin binding;can regulate calcium signal pathway, serotonergic synapse, Kyoto Encyclopedia of Genes and Genomes signal pathway, tryptophan inflammatory mediator regulation pathway, estrogen signal pathway alone or in combination, and play a role in the treatment of allergic rhinitis. Conclusion: Magnoliae Flos combined with Xanthii Fructus can regulate biomolecular network in multiple targets and pathways to treat allergic rhinitis.展开更多
We used 1,1,6,6-tetraphenylhexa-2,4-diyne-1,6-diol（A） as the host molecule to selectively recognize the component 1,8-cineole（B） in the volatile oil of Flos Magnoliae（Xin Yi）. The inclusion compound（A+B） was f...We used 1,1,6,6-tetraphenylhexa-2,4-diyne-1,6-diol（A） as the host molecule to selectively recognize the component 1,8-cineole（B） in the volatile oil of Flos Magnoliae（Xin Yi）. The inclusion compound（A+B） was formed as crystalline, and the structure was confirmed by means of IR, （（）1H NMR）, and single crystal X-ray diffraction, with the 1∶2 molar ratios of the host to the guest. The component 1,8-cineole（B） was selectively isolated from the inclusion compound（A+B） by Kugelrohr vaccum technology, and then further characterized by IR, （）1H NMR and （）13C NMR. The whole components in the volatile oil of Flos Magnoliae（Xin Yi） and the isolation effect of 1,8-cineole isolated from inclusion crystals were analyzed by GC and GC-MS, respectively. The result indicate that the good isolation effect is given by the inclusion method, with purity and yield of the component 1,8-cineole（B） separated from the volatile oil of Flos Magnoliae are 100% and 85%, respectively.展开更多
基金This study was supported by the Discipline Innovation Team Construction Project of the Second Affiliated Hospital of Shaanxi University of Chinese Medicine(2020XKTD-A02).
文摘Objective: To explore the molecular mechanism of Magnoliae Flos and Xanthii Fructus herb pair for allergic rhinitis based on network pharmacology. Methods: From the Traditional Chinese Medicine Systems Pharmacology database, Uniprot database, and Gene Cards database, the relevant chemical constituents information, pharmacokinetic information and hub target of allergic rhinitis were obtained. The protein-protein interaction network was constructed by STRING online database, analyzed and showed by the Cytoscape software. The screened target information was analyzed by the Metascape database for Gene Ontology biological function and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis. Results: Main components of Magnoliae Flos and Xanthii Fructus herb pair, such as aloe-emodin, stigmasterol, beta-sitosterol and Yangambin, depend on the interaction of Nitric Oxide Synthase 3, Tumor Necrosis Factor, Caspase-3 and other functions involve G protein-coupled (amine) receptor activity, RNA polymerase II basic transcription factor binding, protease binding, heme binding, and integrin binding;can regulate calcium signal pathway, serotonergic synapse, Kyoto Encyclopedia of Genes and Genomes signal pathway, tryptophan inflammatory mediator regulation pathway, estrogen signal pathway alone or in combination, and play a role in the treatment of allergic rhinitis. Conclusion: Magnoliae Flos combined with Xanthii Fructus can regulate biomolecular network in multiple targets and pathways to treat allergic rhinitis.
文摘We used 1,1,6,6-tetraphenylhexa-2,4-diyne-1,6-diol（A） as the host molecule to selectively recognize the component 1,8-cineole（B） in the volatile oil of Flos Magnoliae（Xin Yi）. The inclusion compound（A+B） was formed as crystalline, and the structure was confirmed by means of IR, （（）1H NMR）, and single crystal X-ray diffraction, with the 1∶2 molar ratios of the host to the guest. The component 1,8-cineole（B） was selectively isolated from the inclusion compound（A+B） by Kugelrohr vaccum technology, and then further characterized by IR, （）1H NMR and （）13C NMR. The whole components in the volatile oil of Flos Magnoliae（Xin Yi） and the isolation effect of 1,8-cineole isolated from inclusion crystals were analyzed by GC and GC-MS, respectively. The result indicate that the good isolation effect is given by the inclusion method, with purity and yield of the component 1,8-cineole（B） separated from the volatile oil of Flos Magnoliae are 100% and 85%, respectively.
