Objective:To assess the distribution of ABO blood group and their relationship with Plasmodium falciparum(P.falciparum) malaria among febrile outpatients who sought medical attention at Dore Bafeno Health Center,South...Objective:To assess the distribution of ABO blood group and their relationship with Plasmodium falciparum(P.falciparum) malaria among febrile outpatients who sought medical attention at Dore Bafeno Health Center,Southern Ethiopia.Methods:A total of 269 febrile outpatients who visited Dore Bafeno Health Center,Southern Ethiopia,were examined for malaria and also tested for ABO blood groups in January 2010.The blood specimens were collected by finger pricking,stained with Geimsa,and examined microscopically.Positive cases of the parasitemia were counted.CareStart^(TM) Malaria PflPv Combo was also used to test the blood specimens for malaria.ABO blood groups were determined by agglutination test using ERYCLONE antisera.Data on socio-demographic characteristics and treatment status of the participants were also collected.Chi-square and ANOVA tests were used to assess the difference between frequencies and means,respectively.Results:Out of a total of 269 participants,178(66.2%) febrile patients were found to be infected with Plasmodium parasites,among which 146(54.3%),28(10.4%),and 4(1.5%) belonged to P.falciparum,P.vivax,and mixed infections,respectively.All febrile patients were also tested for ABO blood groups and 51.3%,23.5%,21.9%and 3.3%were found to be blood types of 0,A,B and AB,respectively.Both total malaria infection and P.falciparum infection showed significant association with blood types(P<0.05).The proportion of A or B but not 0 phenotypes was higher(P<0.05) in individuals with P.falciparum as compared with non-infected individuals.The chance of having P.falciparum infection in patients with blood groups A,B and AB was 2.5,2.5 and 3.3times more than individuals showing blood 0 phenotypes,respectively.The mean P.falciparum malaria parasitemia for blood groups A,B,AB,and 0 were 3 744/μ L,1 805/ μ L,5 331/μ L,and1 515/μ L,respectively(P<0.01).Conclusions:The present findings indicate that individuals of blood groups A,B and AB are more susceptible to P.falciparum infection as compared with individuals of blood group O.Nevertheless,further in depth studies are required to clearly establish the role that ABO blood group plays in P.falciparum malaria.展开更多
Objective: To compare the safety and efficacy of two compounds of dihydroartemisinin(DHA) -Artekin and Artekin (T) in the treatment of uncomplicated falciparum malaria. Methods:The regimen of 8-tablet for 2 days of Ar...Objective: To compare the safety and efficacy of two compounds of dihydroartemisinin(DHA) -Artekin and Artekin (T) in the treatment of uncomplicated falciparum malaria. Methods:The regimen of 8-tablet for 2 days of Artekin and Artekin (T) were applied to 100 patients with uncomplicated falciparum malaria, who were randomly divided into two groups. Each group contained 50 cases. The cure rate, the mean parasites clearance time, the mean fever clearance and side-effects were observed to assess the safety and efficacy of the compounds used. Results: The mean parasites clearance time was 31. 7±9.0 hours in the Artekin group and 32. 8±8. 8 hours in Artekin (T) group respectively; the mean fever clearance time was 12. 7±7. 2 hours in Artekin group and 16. 5±7. 9 hours in Artekin (T) group; there were no recrudescence case in both groups within the 28 days of follow-up, the cure rates in Artekin group and Artekin (T) groups were 100%. It indicated that the tolerability of both compounds were very good, the side-effects such as nausea, abdominal pain were mild and self-limited. Conclusion: The study preliminarily indicated that the DHA and PQ compounds were of high efficacy, rapid acting and low toxici-ty. Artekin is very promising as a cheap, simple, effective treatment for multi-resistance malaria in Cambodia.展开更多
Background: Drugs that kill or inhibit sexual stages of Plasmodium such as Primaqiune (PQ) could potentially amplify or synergize the impact of first line antimalarials by blocking transmission to mosquitoes. This stu...Background: Drugs that kill or inhibit sexual stages of Plasmodium such as Primaqiune (PQ) could potentially amplify or synergize the impact of first line antimalarials by blocking transmission to mosquitoes. This study examined the effect of Primaquine on gametocyte carriage in the case management of uncomplicated falciparum malaria with artemisinin-based combination therapy (ACT) with the overall purpose of possibly recommending it as an adjunct drug for malaria control. Methods: A total of 181 patients with uncomplicated falciparum malaria, normal glucose-6-phosphate dehydrogenase (G6PD) enzyme levels, and haemoglobin levels ≥ 8 g/dL completed this two-arm randomized blinded clinical trial to test the efficacy of a single dose PQ (0.75 mg/kg) on falciparum gametocytaemia. 88 subjects were assigned to a standard 3-day course of Dihydroartemisinin-Piperaquine (DHP) alone (n = 88) while 93 others had DHP combined with a single dose of PQ on day 3 (n = 93). A 28-day follow-up schedule carried out in the outpatient clinic of a Primary health facility in Vom, Plateau State Nigeria where study participants were seen on days 1, 3, 7 and then weekly to assess the presence of asexual parasites and gametocytes by microscopy. A Kaplan-Meier analysis was employed to determine the survival function of gametocytes on day 3. The data was analyzed using Epi info version 7.1.5. Results: With a gametocyte prevalence of 27.1%, gametocyte carriage rate was lower in the PQ group due to higher probability of clearing gametocytes (Breslow test χ2 = 8.306, df = 1, p = 0.004) and significantly less likely to harbor gametocytes by day 7 when compared to the DHP-alone group (χ2 = 6.218, df = 1, p = 0.013). Conclusion: Addition of single-dose 0.75 mg/kg PQ was associated with reduced gametocyte carriage as a result of faster gametocyte clearance and lower incidence of gametocyte development in DHP-treated patients. PQ as gametocytocidal drug may be useful in combination with artemisinin-based combination therapy (ACT) regimen to clear gametocytes and thereby interrupt malaria transmission to mosquito vector more effectively than ACT alone.展开更多
<strong>Background:</strong> Malaria in pregnancy poses a great health risk to the mother and her fetus and causes abortion, stillbirth, intrauterine growth retardation and low birth weight. The symptoms c...<strong>Background:</strong> Malaria in pregnancy poses a great health risk to the mother and her fetus and causes abortion, stillbirth, intrauterine growth retardation and low birth weight. The symptoms commonly start between 10 - 30 days of age and the symptoms mostly observed are fever, restlessness, drowsiness, jaundice, poor feeding, vomiting, diarrhea, and hepatosplenomegaly. <strong>Aim: </strong>The aim of this study was to diagnose malaria in a neonate admitted to ICU with fever, jaundice and hepatomegaly. <strong>Case Summary: </strong>A 32-day-old female child was admitted to ICU for intermittent high grade fever and rapid breathing, pallor, poor feeding, mild hepatosplenomegaly and physiological jaundice of one-week duration. The mother had malaria two years before while visiting her native country, Afghanistan and was treated with chloroquine for three days. <strong>Conclusion:</strong> High suspicion should be considered in diagnosing malaria during pregnancy to prevent congenital malaria among all neonates who present fever and splenomegaly in malaria endemic areas as well as in women from malaria endemic countries living in non-endemic areas. In this report, we describe the first case of congenital malaria in a child in non-malaria endemic Kuwait.展开更多
Objective:To survey malaria prevalence in Sarbaz from April 2009 to October 2010.Methods: Epidemiological data of 1 464 confirmed malarial patients were analyzed according to demographic status,sex,age,nationality,iso...Objective:To survey malaria prevalence in Sarbaz from April 2009 to October 2010.Methods: Epidemiological data of 1 464 confirmed malarial patients were analyzed according to demographic status,sex,age,nationality,isolated species and residence place.Results:The majority of patients were male 930(64.8%) but 514(35.2%) were female.82.5%of patients were Iranian,14%Pakistani immigrants,and 3.5%Afghan immigrants.Data collected showed that 90% of isolated species were Plasmodium vivax,7.8%Plasmodium falciparum,and 2.2%Plasmodium malariae and mixed species.Conclusions:Therefore,it is crystal clear that refugees should be prohibited by government and controlled by experts in health centers in order to campaign effectively with this life threating disease.展开更多
Objective:To preliminarily investigate the possible role of prostaglandin D_2(PGD_2) in malaria infections.Methods:Blood and urinary samples(n=120 each) were collected from Thai patients with Plasmodium falciparum(P.f...Objective:To preliminarily investigate the possible role of prostaglandin D_2(PGD_2) in malaria infections.Methods:Blood and urinary samples(n=120 each) were collected from Thai patients with Plasmodium falciparum(P.falciparum) with moderate(n=26) and high(n=4) parasitemia,patients with Plasmodium vivax(P.vivax)(n=30),patients with fever associated with other infections(n=30),and healthy subjects(n=30).PGD_2 concentrations in plasma and urinary samples of healthy subjects,patients with fever associated with other infections and patients with malaria were determined using Prostaglandin D2-MOX express EIA kit(Cayman Chemical,USA).Results:The possible association between PGD_2 and malaria infections is clearly demonstrated with PGD_2 concentration in urine.The urinary PGD_2 concentrations were relatively high(about 5-fold) in patients with P.falciparum with moderate parasitemia and P.vivax infections compared with other groups.Furthermore,the concentration in patients with P.falciparum with moderate parasitemia and P.vivax infection were significantly higher than that in healthy subjects and patients with fever associated with other infections.Conclusions:Urinary PGD_2 concentrations may offer a more dependable and useful tool for predicting malaria severity.Confirmation is this preliminary finding is required with a larger sample size.展开更多
Objective: To investigate clinically severe malaria patients with Plasmodium falciparum(P. falciparum), Plasmodium vivax(P. vivax) and mixed species infections.Methods: This study was conducted at Dr. Saiful Anwar Gen...Objective: To investigate clinically severe malaria patients with Plasmodium falciparum(P. falciparum), Plasmodium vivax(P. vivax) and mixed species infections.Methods: This study was conducted at Dr. Saiful Anwar General Hospital, Malang,Indonesia, from December 2011 to May 2013. Twenty nine patients(mean age of 41 years, 22% female), who suffered from severe malaria according to World Health Organization criteria(major and minor) and other criteria based on previous studies, were selected by consecutive sampling. Blood samples were obtained at admission from peripheral blood for microscopic diagnostic, nested PCR and laboratory examination of blood chemistry. Laboratory results were compared between the groups and correlated to each other.Results: From 29 samples, eight(28%) were diagnosed as P. falciparum mono-infection,12(41%) as P. vivax mono-infection and nine(31%) as mixed infections, confirmed by PCR. Cerebral malaria occurred in P. falciparum or mixed species infection only. Parasitaemia was highest in P. falciparum mono-infection. Mean haemoglobin was significantly lower in P. falciparum than P. vivax infection(P = 0.01). Mean thrombocyte count(77 138/m L) was low in all groups. Mean urea, creatinine, total and direct bilirubin were significantly higher in P. falciparum mono-infection compared to other groups, whereas aspartate aminotransferase and alanine aminotransferase showed no significant differences. Parasitaemia was positively correlated with an increase in urea, creatinine, bilirubin and leucocytosis in all species.Conclusions: Both Plasmodium species can solely or in combination cause severe malaria. Mixed infection was generally more benign than P. falciparum mono-infection and seemed to have some protective effects.展开更多
The research scenario for malaria has improved in the last three decades to understand the epidemiology and host immune responses to plasmodial infection.Due to the augmented episodes of resistance development against...The research scenario for malaria has improved in the last three decades to understand the epidemiology and host immune responses to plasmodial infection.Due to the augmented episodes of resistance development against the commonly used antimalarials in plasmodium parasites,especially in Plasmodium falciparum,neutralization of infection through effective vaccine(s) remains the feasible alternative in malaria control.In this direction,lot of attention was paid towards the identification of stage specific malaria antigens targeted by host ’s immune system.Preparation of synthetic or recombinant peptides and evaluation of their immunogenecity in naturally occurring antibody response were also given much importance,as these studies could help in finding potential candidates for future malaria vaccine(s).Attention was also paid.on the pathogenic consequences of antibody formation in malaria infection as polyclonal activation of B cells,which is a very prominent feature in malaria infection.Formation of circulating immune complexes in chronic malaria infection was also viewed as pathogenic parameter of severe malaria.The present survey focuses mainly on protective and pathogenic aspects of malaria antibodies(eliciting against various,stage specific antigens),and future research plan in antibodymediated immune response.展开更多
Background:Ethiopia is one of the African countries where Plasmodium falciparum and P.vivax co-exist.Monitoring and evaluation of current malaria transmission status is an important component of malaria control as it ...Background:Ethiopia is one of the African countries where Plasmodium falciparum and P.vivax co-exist.Monitoring and evaluation of current malaria transmission status is an important component of malaria control as it is a measure of the success of ongoing interventions and guides the planning of future control and elimination efforts.Main text:We evaluated changes in malaria control policy in Ethiopia,and reviewed dynamics of country-wide confirmed and clinical malaria cases by Plasmodium species and reported deaths for all ages and less than five years from 2001 to 2016.Districts level annual parasite incidence was analysed to characterize the malaria transmission stratification as implemented by the Ministry of Health.We found that Ethiopia has experienced major changes from 2003 to 2005 and subsequent adjustment in malaria diagnosis,treatment and vector control policy.Malaria interventions have been intensified represented by the increased insecticide treated net(ITN)and indoor residual spraying(IRS)coverage,improved health services and improved malaria diagnosis.However,countrywide ITN and IRS coverages were low,with 64%ITN coverage in 2016 and IRS coverage of 92.5%in 2016 and only implemented in epidemic-prone areas of>2500 m elevation.Clinical malaria incidence rate dropped from an average of 43.1 cases per 1000 population annually between 2001 and 2010 to 29.0 cases per 1000 population annually between 2011 and 2016.Malaria deaths decreased from 2.1 deaths per 100000 people annually between 2001 and 2010 to 1.1 deaths per 100000 people annually between 2011 to 2016.There was shrinkage in the malaria transmission map and high transmission is limited mainly to the western international border area.Proportion of P.falciparum malaria remained nearly unchanged from 2000 to 2016 indicating further efforts are needed to suppress transmission.Conclusions:Malaria morbidity and mortality have been significantly reduced in Ethiopia since 2001,however,malaria case incidence is still high,and there were major gaps between ITN ownership and compliance in malarious areas.Additional efforts are needed to target the high transmission area of western Ethiopia to sustain the achievements made to date.展开更多
Background:Plasmodium falciparum malaria is endemic in the southern sahelian zone of Mauritania where intense internal and trans-border human and livestock movement occurs.The risk of importation and spread of drugres...Background:Plasmodium falciparum malaria is endemic in the southern sahelian zone of Mauritania where intense internal and trans-border human and livestock movement occurs.The risk of importation and spread of drugresistant parasites need to be regularly assessed in this region.The objective of the study was to assess the recent malaria situation near the Mauritania-Mali border.Methods:Between February 2015 and December 2017,patients with fever or history of fever during the previous 48 h,presenting at the health centre of Kobeni city,were screened for malaria using a rapid diagnostic test(RDT)and microscopic examination of blood smears.The diagnosis was later confirmed by PCR.Cohen’s kappa statistics was used to estimate the degree of agreement between diagnostic methods.Fisher’s exact test was used to compare proportions.The odds ratio was calculated to measure the association between the use of bed nets and malaria infection.Results:A total of 2326 febrile patients(mean age,20.2 years)were screened for malaria.The presence of malaria parasites was detected by RDT and microscopy in 53.0%and 49.3%of febrile patients,respectively,and was confirmed by PCR in 59.7%(45 missing data).Of 1361 PCR-positive samples,1205(88.5%)were P.falciparum,47(3.5%)P.vivax,and 99(7.3%)P.falciparum-P.vivax mixed infection.Malaria transmission occurred mostly during and shortly after the rainy season.The annual rainfall was relatively low in 2016(267 mm)and 2017(274 mm),compared to 2015(448 mm),and coincided with a decline in malaria prevalence in 2016–2017.Although 71.8%of febrile patients reported to possess at least one bed net in the household in our questionnaire,its reported use was not protective against malaria infection(odds ratio:1.1,95%CI:0.91–1.32).Conclusions:Our study confirmed that P.falciparum is the dominant species in the sahelian zone and that malaria transmission is seasonal and associated with rainfall in this zone.The application of the current national policy based on rapid and reliable malaria diagnosis,case management with artemisinin-based combination therapy,intermittent preventive treatment for pregnant women,distribution and use of long-lasting insecticide impregnated bed nets,and the planned introduction of seasonal malaria chemoprevention for all children under 6 years old is expected to sustainably reduce malaria transmission in this zone.展开更多
Background:This study aimed to evaluate the prevalence and alteration of hematological parameters in malaria patients with a glucose-6-phosphate dehydrogenase(G6PD)deficiency,in the western region of Thailand,an endem...Background:This study aimed to evaluate the prevalence and alteration of hematological parameters in malaria patients with a glucose-6-phosphate dehydrogenase(G6PD)deficiency,in the western region of Thailand,an endemic region for malaria.Methods:Data about patients with malaria hospitalized between 2013 and 2015 were collected.Clinical and sociodemographic characteristics such as age and gender,diagnosis on admission,and parasitological results were mined from medical records of the laboratory unit of the Phop Phra Hospital in Tak Province,Thailand.Venous blood samples were collected at the time of admission to hospital to determine G6PD deficiency by fluorescence spot test and detect malaria parasites by thick and thin film examination.Other data such as complete blood count and parasite density were also collected and analyzed.Results:Among the 245 malaria cases,28(11.4%)were diagnosed as Plasmodium falciparum infections and 217 cases(88.6%)were diagnosed as P.vivax infections.Seventeen(6.9%)patients had a G6PD deficiency and 228(93.1%)patients did not have a G6PD deficiency.Prevalence of male patients with G6PD deficiency was higher than that of female patients(P<0.05,OR=5.167).Among the patients with a G6PD deficiency,two(11.8%)were infected with P.falciparum,while the remaining were infected with P.vivax.Malaria patients with a G6PD deficiency have higher monocyte counts(0.6×10^(3)/μL)than those without a G6PD deficiency(0.33×10^(3)/μL)(P<0.05,OR=5.167).Univariate and multivariate analyses also confirmed that malaria patients with a G6PD deficiency have high monocyte counts.The association between G6PD status and monocyte counts was independent of age,gender,nationality,Plasmodium species,and parasite density(P<0.005).Conclusion:This study showed a prevalence of G6PD deficiency in a malaria-endemic area.This study also supported the assertion that patients with G6PD-deficient red blood cells had no protection against the P.falciparum infection.In addition,malaria patients with a G6PD deficiency had higher monocyte counts than those without a G6PD deficiency.These findings will help to recognize and diagnose malaria patients with a G6PD deficiency,as well as to identify the risks and protective factors against malaria in endemic areas.展开更多
基金Supported by School of Graduate Studies through Aklilu LemmaInstitute of Pathobiology,Addis Ababa University(No:RDP/Py-014/09)
文摘Objective:To assess the distribution of ABO blood group and their relationship with Plasmodium falciparum(P.falciparum) malaria among febrile outpatients who sought medical attention at Dore Bafeno Health Center,Southern Ethiopia.Methods:A total of 269 febrile outpatients who visited Dore Bafeno Health Center,Southern Ethiopia,were examined for malaria and also tested for ABO blood groups in January 2010.The blood specimens were collected by finger pricking,stained with Geimsa,and examined microscopically.Positive cases of the parasitemia were counted.CareStart^(TM) Malaria PflPv Combo was also used to test the blood specimens for malaria.ABO blood groups were determined by agglutination test using ERYCLONE antisera.Data on socio-demographic characteristics and treatment status of the participants were also collected.Chi-square and ANOVA tests were used to assess the difference between frequencies and means,respectively.Results:Out of a total of 269 participants,178(66.2%) febrile patients were found to be infected with Plasmodium parasites,among which 146(54.3%),28(10.4%),and 4(1.5%) belonged to P.falciparum,P.vivax,and mixed infections,respectively.All febrile patients were also tested for ABO blood groups and 51.3%,23.5%,21.9%and 3.3%were found to be blood types of 0,A,B and AB,respectively.Both total malaria infection and P.falciparum infection showed significant association with blood types(P<0.05).The proportion of A or B but not 0 phenotypes was higher(P<0.05) in individuals with P.falciparum as compared with non-infected individuals.The chance of having P.falciparum infection in patients with blood groups A,B and AB was 2.5,2.5 and 3.3times more than individuals showing blood 0 phenotypes,respectively.The mean P.falciparum malaria parasitemia for blood groups A,B,AB,and 0 were 3 744/μ L,1 805/ μ L,5 331/μ L,and1 515/μ L,respectively(P<0.01).Conclusions:The present findings indicate that individuals of blood groups A,B and AB are more susceptible to P.falciparum infection as compared with individuals of blood group O.Nevertheless,further in depth studies are required to clearly establish the role that ABO blood group plays in P.falciparum malaria.
文摘Objective: To compare the safety and efficacy of two compounds of dihydroartemisinin(DHA) -Artekin and Artekin (T) in the treatment of uncomplicated falciparum malaria. Methods:The regimen of 8-tablet for 2 days of Artekin and Artekin (T) were applied to 100 patients with uncomplicated falciparum malaria, who were randomly divided into two groups. Each group contained 50 cases. The cure rate, the mean parasites clearance time, the mean fever clearance and side-effects were observed to assess the safety and efficacy of the compounds used. Results: The mean parasites clearance time was 31. 7±9.0 hours in the Artekin group and 32. 8±8. 8 hours in Artekin (T) group respectively; the mean fever clearance time was 12. 7±7. 2 hours in Artekin group and 16. 5±7. 9 hours in Artekin (T) group; there were no recrudescence case in both groups within the 28 days of follow-up, the cure rates in Artekin group and Artekin (T) groups were 100%. It indicated that the tolerability of both compounds were very good, the side-effects such as nausea, abdominal pain were mild and self-limited. Conclusion: The study preliminarily indicated that the DHA and PQ compounds were of high efficacy, rapid acting and low toxici-ty. Artekin is very promising as a cheap, simple, effective treatment for multi-resistance malaria in Cambodia.
文摘Background: Drugs that kill or inhibit sexual stages of Plasmodium such as Primaqiune (PQ) could potentially amplify or synergize the impact of first line antimalarials by blocking transmission to mosquitoes. This study examined the effect of Primaquine on gametocyte carriage in the case management of uncomplicated falciparum malaria with artemisinin-based combination therapy (ACT) with the overall purpose of possibly recommending it as an adjunct drug for malaria control. Methods: A total of 181 patients with uncomplicated falciparum malaria, normal glucose-6-phosphate dehydrogenase (G6PD) enzyme levels, and haemoglobin levels ≥ 8 g/dL completed this two-arm randomized blinded clinical trial to test the efficacy of a single dose PQ (0.75 mg/kg) on falciparum gametocytaemia. 88 subjects were assigned to a standard 3-day course of Dihydroartemisinin-Piperaquine (DHP) alone (n = 88) while 93 others had DHP combined with a single dose of PQ on day 3 (n = 93). A 28-day follow-up schedule carried out in the outpatient clinic of a Primary health facility in Vom, Plateau State Nigeria where study participants were seen on days 1, 3, 7 and then weekly to assess the presence of asexual parasites and gametocytes by microscopy. A Kaplan-Meier analysis was employed to determine the survival function of gametocytes on day 3. The data was analyzed using Epi info version 7.1.5. Results: With a gametocyte prevalence of 27.1%, gametocyte carriage rate was lower in the PQ group due to higher probability of clearing gametocytes (Breslow test χ2 = 8.306, df = 1, p = 0.004) and significantly less likely to harbor gametocytes by day 7 when compared to the DHP-alone group (χ2 = 6.218, df = 1, p = 0.013). Conclusion: Addition of single-dose 0.75 mg/kg PQ was associated with reduced gametocyte carriage as a result of faster gametocyte clearance and lower incidence of gametocyte development in DHP-treated patients. PQ as gametocytocidal drug may be useful in combination with artemisinin-based combination therapy (ACT) regimen to clear gametocytes and thereby interrupt malaria transmission to mosquito vector more effectively than ACT alone.
文摘<strong>Background:</strong> Malaria in pregnancy poses a great health risk to the mother and her fetus and causes abortion, stillbirth, intrauterine growth retardation and low birth weight. The symptoms commonly start between 10 - 30 days of age and the symptoms mostly observed are fever, restlessness, drowsiness, jaundice, poor feeding, vomiting, diarrhea, and hepatosplenomegaly. <strong>Aim: </strong>The aim of this study was to diagnose malaria in a neonate admitted to ICU with fever, jaundice and hepatomegaly. <strong>Case Summary: </strong>A 32-day-old female child was admitted to ICU for intermittent high grade fever and rapid breathing, pallor, poor feeding, mild hepatosplenomegaly and physiological jaundice of one-week duration. The mother had malaria two years before while visiting her native country, Afghanistan and was treated with chloroquine for three days. <strong>Conclusion:</strong> High suspicion should be considered in diagnosing malaria during pregnancy to prevent congenital malaria among all neonates who present fever and splenomegaly in malaria endemic areas as well as in women from malaria endemic countries living in non-endemic areas. In this report, we describe the first case of congenital malaria in a child in non-malaria endemic Kuwait.
文摘Objective:To survey malaria prevalence in Sarbaz from April 2009 to October 2010.Methods: Epidemiological data of 1 464 confirmed malarial patients were analyzed according to demographic status,sex,age,nationality,isolated species and residence place.Results:The majority of patients were male 930(64.8%) but 514(35.2%) were female.82.5%of patients were Iranian,14%Pakistani immigrants,and 3.5%Afghan immigrants.Data collected showed that 90% of isolated species were Plasmodium vivax,7.8%Plasmodium falciparum,and 2.2%Plasmodium malariae and mixed species.Conclusions:Therefore,it is crystal clear that refugees should be prohibited by government and controlled by experts in health centers in order to campaign effectively with this life threating disease.
基金funded by Thailand Research Fund-Thammasat University Joint Fund and Graduated Student Grant to P.Thongdee(No.PHD/0365/2552)
文摘Objective:To preliminarily investigate the possible role of prostaglandin D_2(PGD_2) in malaria infections.Methods:Blood and urinary samples(n=120 each) were collected from Thai patients with Plasmodium falciparum(P.falciparum) with moderate(n=26) and high(n=4) parasitemia,patients with Plasmodium vivax(P.vivax)(n=30),patients with fever associated with other infections(n=30),and healthy subjects(n=30).PGD_2 concentrations in plasma and urinary samples of healthy subjects,patients with fever associated with other infections and patients with malaria were determined using Prostaglandin D2-MOX express EIA kit(Cayman Chemical,USA).Results:The possible association between PGD_2 and malaria infections is clearly demonstrated with PGD_2 concentration in urine.The urinary PGD_2 concentrations were relatively high(about 5-fold) in patients with P.falciparum with moderate parasitemia and P.vivax infections compared with other groups.Furthermore,the concentration in patients with P.falciparum with moderate parasitemia and P.vivax infection were significantly higher than that in healthy subjects and patients with fever associated with other infections.Conclusions:Urinary PGD_2 concentrations may offer a more dependable and useful tool for predicting malaria severity.Confirmation is this preliminary finding is required with a larger sample size.
基金Supported by Faculty of Medicine of the University of Brawijaya for the funding(Grant No.101388/UN10.7/PHK PKPD/2012)
文摘Objective: To investigate clinically severe malaria patients with Plasmodium falciparum(P. falciparum), Plasmodium vivax(P. vivax) and mixed species infections.Methods: This study was conducted at Dr. Saiful Anwar General Hospital, Malang,Indonesia, from December 2011 to May 2013. Twenty nine patients(mean age of 41 years, 22% female), who suffered from severe malaria according to World Health Organization criteria(major and minor) and other criteria based on previous studies, were selected by consecutive sampling. Blood samples were obtained at admission from peripheral blood for microscopic diagnostic, nested PCR and laboratory examination of blood chemistry. Laboratory results were compared between the groups and correlated to each other.Results: From 29 samples, eight(28%) were diagnosed as P. falciparum mono-infection,12(41%) as P. vivax mono-infection and nine(31%) as mixed infections, confirmed by PCR. Cerebral malaria occurred in P. falciparum or mixed species infection only. Parasitaemia was highest in P. falciparum mono-infection. Mean haemoglobin was significantly lower in P. falciparum than P. vivax infection(P = 0.01). Mean thrombocyte count(77 138/m L) was low in all groups. Mean urea, creatinine, total and direct bilirubin were significantly higher in P. falciparum mono-infection compared to other groups, whereas aspartate aminotransferase and alanine aminotransferase showed no significant differences. Parasitaemia was positively correlated with an increase in urea, creatinine, bilirubin and leucocytosis in all species.Conclusions: Both Plasmodium species can solely or in combination cause severe malaria. Mixed infection was generally more benign than P. falciparum mono-infection and seemed to have some protective effects.
文摘The research scenario for malaria has improved in the last three decades to understand the epidemiology and host immune responses to plasmodial infection.Due to the augmented episodes of resistance development against the commonly used antimalarials in plasmodium parasites,especially in Plasmodium falciparum,neutralization of infection through effective vaccine(s) remains the feasible alternative in malaria control.In this direction,lot of attention was paid towards the identification of stage specific malaria antigens targeted by host ’s immune system.Preparation of synthetic or recombinant peptides and evaluation of their immunogenecity in naturally occurring antibody response were also given much importance,as these studies could help in finding potential candidates for future malaria vaccine(s).Attention was also paid.on the pathogenic consequences of antibody formation in malaria infection as polyclonal activation of B cells,which is a very prominent feature in malaria infection.Formation of circulating immune complexes in chronic malaria infection was also viewed as pathogenic parameter of severe malaria.The present survey focuses mainly on protective and pathogenic aspects of malaria antibodies(eliciting against various,stage specific antigens),and future research plan in antibodymediated immune response.
基金This study was supported by grants from the National Institutes of Health(D43 TW001505,R01 A1050243 and U19 AI129326 to G.Yan).
文摘Background:Ethiopia is one of the African countries where Plasmodium falciparum and P.vivax co-exist.Monitoring and evaluation of current malaria transmission status is an important component of malaria control as it is a measure of the success of ongoing interventions and guides the planning of future control and elimination efforts.Main text:We evaluated changes in malaria control policy in Ethiopia,and reviewed dynamics of country-wide confirmed and clinical malaria cases by Plasmodium species and reported deaths for all ages and less than five years from 2001 to 2016.Districts level annual parasite incidence was analysed to characterize the malaria transmission stratification as implemented by the Ministry of Health.We found that Ethiopia has experienced major changes from 2003 to 2005 and subsequent adjustment in malaria diagnosis,treatment and vector control policy.Malaria interventions have been intensified represented by the increased insecticide treated net(ITN)and indoor residual spraying(IRS)coverage,improved health services and improved malaria diagnosis.However,countrywide ITN and IRS coverages were low,with 64%ITN coverage in 2016 and IRS coverage of 92.5%in 2016 and only implemented in epidemic-prone areas of>2500 m elevation.Clinical malaria incidence rate dropped from an average of 43.1 cases per 1000 population annually between 2001 and 2010 to 29.0 cases per 1000 population annually between 2011 and 2016.Malaria deaths decreased from 2.1 deaths per 100000 people annually between 2001 and 2010 to 1.1 deaths per 100000 people annually between 2011 to 2016.There was shrinkage in the malaria transmission map and high transmission is limited mainly to the western international border area.Proportion of P.falciparum malaria remained nearly unchanged from 2000 to 2016 indicating further efforts are needed to suppress transmission.Conclusions:Malaria morbidity and mortality have been significantly reduced in Ethiopia since 2001,however,malaria case incidence is still high,and there were major gaps between ITN ownership and compliance in malarious areas.Additional efforts are needed to target the high transmission area of western Ethiopia to sustain the achievements made to date.
文摘Background:Plasmodium falciparum malaria is endemic in the southern sahelian zone of Mauritania where intense internal and trans-border human and livestock movement occurs.The risk of importation and spread of drugresistant parasites need to be regularly assessed in this region.The objective of the study was to assess the recent malaria situation near the Mauritania-Mali border.Methods:Between February 2015 and December 2017,patients with fever or history of fever during the previous 48 h,presenting at the health centre of Kobeni city,were screened for malaria using a rapid diagnostic test(RDT)and microscopic examination of blood smears.The diagnosis was later confirmed by PCR.Cohen’s kappa statistics was used to estimate the degree of agreement between diagnostic methods.Fisher’s exact test was used to compare proportions.The odds ratio was calculated to measure the association between the use of bed nets and malaria infection.Results:A total of 2326 febrile patients(mean age,20.2 years)were screened for malaria.The presence of malaria parasites was detected by RDT and microscopy in 53.0%and 49.3%of febrile patients,respectively,and was confirmed by PCR in 59.7%(45 missing data).Of 1361 PCR-positive samples,1205(88.5%)were P.falciparum,47(3.5%)P.vivax,and 99(7.3%)P.falciparum-P.vivax mixed infection.Malaria transmission occurred mostly during and shortly after the rainy season.The annual rainfall was relatively low in 2016(267 mm)and 2017(274 mm),compared to 2015(448 mm),and coincided with a decline in malaria prevalence in 2016–2017.Although 71.8%of febrile patients reported to possess at least one bed net in the household in our questionnaire,its reported use was not protective against malaria infection(odds ratio:1.1,95%CI:0.91–1.32).Conclusions:Our study confirmed that P.falciparum is the dominant species in the sahelian zone and that malaria transmission is seasonal and associated with rainfall in this zone.The application of the current national policy based on rapid and reliable malaria diagnosis,case management with artemisinin-based combination therapy,intermittent preventive treatment for pregnant women,distribution and use of long-lasting insecticide impregnated bed nets,and the planned introduction of seasonal malaria chemoprevention for all children under 6 years old is expected to sustainably reduce malaria transmission in this zone.
文摘Background:This study aimed to evaluate the prevalence and alteration of hematological parameters in malaria patients with a glucose-6-phosphate dehydrogenase(G6PD)deficiency,in the western region of Thailand,an endemic region for malaria.Methods:Data about patients with malaria hospitalized between 2013 and 2015 were collected.Clinical and sociodemographic characteristics such as age and gender,diagnosis on admission,and parasitological results were mined from medical records of the laboratory unit of the Phop Phra Hospital in Tak Province,Thailand.Venous blood samples were collected at the time of admission to hospital to determine G6PD deficiency by fluorescence spot test and detect malaria parasites by thick and thin film examination.Other data such as complete blood count and parasite density were also collected and analyzed.Results:Among the 245 malaria cases,28(11.4%)were diagnosed as Plasmodium falciparum infections and 217 cases(88.6%)were diagnosed as P.vivax infections.Seventeen(6.9%)patients had a G6PD deficiency and 228(93.1%)patients did not have a G6PD deficiency.Prevalence of male patients with G6PD deficiency was higher than that of female patients(P<0.05,OR=5.167).Among the patients with a G6PD deficiency,two(11.8%)were infected with P.falciparum,while the remaining were infected with P.vivax.Malaria patients with a G6PD deficiency have higher monocyte counts(0.6×10^(3)/μL)than those without a G6PD deficiency(0.33×10^(3)/μL)(P<0.05,OR=5.167).Univariate and multivariate analyses also confirmed that malaria patients with a G6PD deficiency have high monocyte counts.The association between G6PD status and monocyte counts was independent of age,gender,nationality,Plasmodium species,and parasite density(P<0.005).Conclusion:This study showed a prevalence of G6PD deficiency in a malaria-endemic area.This study also supported the assertion that patients with G6PD-deficient red blood cells had no protection against the P.falciparum infection.In addition,malaria patients with a G6PD deficiency had higher monocyte counts than those without a G6PD deficiency.These findings will help to recognize and diagnose malaria patients with a G6PD deficiency,as well as to identify the risks and protective factors against malaria in endemic areas.