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Establishment on Isolated Microspore Culture Optimized System for Restorer of New Cytoplasmic Male Sterile (NER) of Brassica napus L.
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作者 李浩杰 蒲晓斌 +5 位作者 张锦芳 张德发 夏凊 石化娟 蒋俊 蒋梁材 《Agricultural Science & Technology》 CAS 2010年第1期182-185,共4页
[Objective]The aim was to establish optimized system for NER isolated microspore culture of Brassica napus L.. [Method]Twenty varieties of NER of Brassica napus were grown under uncontrolled temperature and light cond... [Objective]The aim was to establish optimized system for NER isolated microspore culture of Brassica napus L.. [Method]Twenty varieties of NER of Brassica napus were grown under uncontrolled temperature and light conditions,and their isolated microspore from anthers were used as explants in vitro culture. The influencing factors of microspore culture were preliminarily studied. [Result]The difference of genotypes was important influencing factors to embryoid yield. The embryoid yield increased by supplementing with 6-BA and NAA,culturing in solid-liquid double layer medium with activated charcoal; The difference was not significant of embryoid yield between culturing in medium supplemented with colchicines and the CK. The rates of cotyledonous embryoids directly developed into normal plantlets increased through enriching with 0.1-0.2 mg/L NAA and being treated on slim illumination two days before being inducted into normal plantlets. [Conclusion]The technical system of microspore culture of restorer of new cytoplasmic male sterile (NER) was established,which and lays a foundation for accelerating genotype purification of NER introgressive line. 展开更多
关键词 Brassica napus L. Restorer of new cytoplasmic male sterile (NER) Isolated microspore culture Technical system
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Effects of circumcision on male sexual functions: systematic review and meta-analysis 被引量:11
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作者 Wei Liu Jian-Zhong Wang +2 位作者 Romel Wazir Xuan Yue Kun-Jie Wang 《Asian Journal of Andrology》 SCIE CAS CSCD 2013年第5期662-666,共5页
This meta-analysis was performed to assess sexual functions following adult male circumcision. We searched the Cochrane Central Register of Controlled Trials, PUBMED, EMBASE, the Cochrane Database of Systematic Review... This meta-analysis was performed to assess sexual functions following adult male circumcision. We searched the Cochrane Central Register of Controlled Trials, PUBMED, EMBASE, the Cochrane Database of Systematic Review and Web of Science from their inception until January 2013 to identify all eligible studies that reported on men's sexual function after circumcision. The Cochrane Collaboration's RevMan 5.2 software was employed for data analysis, and the fixed or the random effect model was selected depending on the proportion of heterogeneity. We identified 10 studies, which described a total of 9317 circumcised and 9423 uncircumcised men who were evaluated for the association of circumcision with male sexual function. There were no significant differences in sexual desire (odds ratio (OR): 0.99; 95% confidence interval (CI): 0.92-1.06), dyspareunia (OR: 1.12; 95% Ch 0.52-2.44), premature ejaculation (OR. 1.13; 95% Ch 0.83-1.54), ejaculation latency time (OR: 1.33; 95% Ch 0.69-1.97), erectile dysfunctions (OR: 0.90; 95% Ch 0.65-1.25) and orgasm difficulties (OR: 0.97; 95% Ch 0.83-1.13). These findings suggest that circumcision is unlikely to adversely affect male sexual functions. However, these results should be evaluated in light of the low quality of the existing evidence and the significant heterogeneity across the various studies. Well-designed and prospective studies are required for a further understanding of this topic. 展开更多
关键词 COMPLICATIONS male circumcision REVIEW sexual function
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Double versus single homologous intrauterine insemination for male factor infertility: a systematic review and meta-analysis 被引量:9
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作者 Apostolos Zavos Alexandros Daoonte +4 位作者 Antonios Garas Christina Verykouki Evangelos Papanikolao~ Georgios Anifandis Nikolaos P Polyzos 《Asian Journal of Andrology》 SCIE CAS CSCD 2013年第4期533-538,I0010,共7页
Male factor infertility affects 30%-50% of infertile couples worldwide, and there is an increasing interest in the optimal management of these patients. In studies comparing double and single intrauterine insemination... Male factor infertility affects 30%-50% of infertile couples worldwide, and there is an increasing interest in the optimal management of these patients. In studies comparing double and single intrauterine insemination (IUI), a trend towards higher pregnancy rates in couples with male factor infertility was observed. Therefore, we set out to perform a meta-analysis to examine the superiority of double versus single IUI with the male partner's sperm in couples with male factor infertility. An odds ratio (OR) of 95% confidence intervals (CIs) was calculated for the pregnancy rate. Outcomes were analysed by using the ManteI-Haesel or DerSimonian-Laird model accordingto the heterogeneity of the results. Overall, five trials involving 1125 IUI cycles were included in the meta-analysis. There was a two-fold increase in pregnancies after a cycle with a double IUI compared with a cycle with a single IUI (OR. 2.0; 95% CI. 1.07-3.75; P〈O.03). Nevertheless, this result was mainly attributed to the presence of a large trial that weighted as almost 50% in the overall analysis. Sensitivity analysis, excluding this large trial, revealed only a trend towards higher pregnancy rates among double IUI cycles (OR. 1.58; 95% CI. 0.59-4.21), but without statistical significance (P=0.20). Our systematic review highlights that the available evidence regarding the use of double IUI in couples with male factor infertility is fragmentary and weak. Although there may be a trend towards higher pregnancy rates when the number of IUIs per cycle is increased, further large and well-designed randomized trials are needed to provide solid evidence toide current clinical practice. 展开更多
关键词 HOMOLOGOUS intrauterine insemination male infertility META-ANALYSIS systematic review
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Generation of male-sterile soybean lines with the CRISPR/Cas9 system 被引量:6
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作者 Xiao Chen Suxin Yang +5 位作者 Yaohua Zhang Xiaobin Zhu Xinjing Yang Chunbao Zhang Haiyan Li Xianzhong Feng 《The Crop Journal》 SCIE CSCD 2021年第6期1270-1277,共8页
Soybean [Glycine max(L.) Merr.] provides a rich source of plant protein and oil worldwide. The commercial use of transgenic technology in soybean has become a classical example of the application of biotechnology to c... Soybean [Glycine max(L.) Merr.] provides a rich source of plant protein and oil worldwide. The commercial use of transgenic technology in soybean has become a classical example of the application of biotechnology to crop improvement. Although genetically modified soybeans have achieved commercial success,hybrid soybean breeding is also a potential way to increase soybean yield. Soybean cytoplasmic malesterile(CMS) lines have been used in three-line hybrid breeding systems, but their application to exploiting soybean heterosis has been limited by rare germplasm resource of sterile lines. The generation of various genetic diversity male-sterile soybean lines will help to overcome the shortcoming. In this study,we used targeted editing of AMS homologs in soybean by CRISPR/Cas9 technology for the first time to generate stable male-sterile lines. Targeted editing of GmAMS1 resulted in a male-sterile phenotype,while editing of GmAMS2 failed to produce male-sterile lines. GmAMS1 functions not only in the formation of the pollen wall but also in the controlling the degradation of the soybean tapetum.CRISPR/Cas9 technology could be used to rapidly produce stable male-sterile lines, providing new sterile-line materials for soybean hybrid breeding systems. 展开更多
关键词 CRISPR/Cas9 SOYBEAN TAPETUM Gmams male sterility
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The effectiveness and safety of acupuncture for poor semen quality in infertile males: a systematic review and meta-analysis 被引量:3
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作者 Ui Min Jerng Jun-Young Jo +2 位作者 Seunghoon Lee Jin-Moo Lee Ohmin Kwon 《Asian Journal of Andrology》 SCIE CAS CSCD 2014年第6期884-891,共8页
The aim of this review is to evaluate the effectiveness and safety of acupuncture for poor semen quality in infertile men. We searched for relevant trials registered up to May 2013 in 14 databases. We selected randomi... The aim of this review is to evaluate the effectiveness and safety of acupuncture for poor semen quality in infertile men. We searched for relevant trials registered up to May 2013 in 14 databases. We selected randomized controlled trials (RCTs) that compared acupuncture, with or without additional treatment, against placebo, sham, no treatment, or the same additional treatment. Two reviewers independently performed the study selection, data extraction, risk of bias and reporting quality appraisal. Risk of bias and reporting quality were appraised by the Cochrane risk of bias tool, the consolidated standards of reporting trials and Standards for Reporting Interventions in Clinical Trials of Acupuncture. The outcomes were sperm motility, sperm concentration, pregnancy rate, and adverse events. Pregnancy was defined as a positive pregnancy test. Four RCTs met the eligibility criteria. Acupuncture increased the percentage of sperm with rapid progression (mean difference - 6.35, 95% confidence interval (CI). 4.38-8.32, P〈 0.00001) and sperm concentration (mean difference - 6.42, 95% CI. 4.91-7.92, P〈 0.00001), but these two outcomes were substantially heterogeneous among the studies (F = 72% and 58%, respectively). No differences in pregnancy rate were found between acupuncture and control groups (odds ratio 1.60, 95% CI. 0.70-3.69, P= 0.27, F = 0%). No participants experienced adverse events. The current evidence showing that acupuncture might improve poor semen quality is insufficient because of the small number of studies, inadequacy of procedures and/or insufficient information for semen analysis, high levels of heterogeneity, high risk of bias, and poor quality of reporting. Further large, well-designed RCTs are required. 展开更多
关键词 ACUPUNCTURE ASTHENOZOOSPERMIA male infertility OLIGOZOOSPERMIA sperm quality
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Effect of Aqueous Extract of <i>Phoenix dactylifera</i>Pollen on Dopamine System of Nucleus Accumbens in Male Rats 被引量:2
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作者 Ali Abedi Seyyed Morteza Karimian +2 位作者 Mohsen Parviz Perham Mohammadi Hamid Reza Sadeghipour Roudsari 《Neuroscience & Medicine》 2014年第1期49-59,共11页
Background: Dopamine has been known to facilitate male sexual function. Methods: The effect of aqueous extract (140 mg/kg) of Phoenix dactylifera date palm pollen on sexual behavior and determining of dopamine transmi... Background: Dopamine has been known to facilitate male sexual function. Methods: The effect of aqueous extract (140 mg/kg) of Phoenix dactylifera date palm pollen on sexual behavior and determining of dopamine transmission in the nucleus accumbens was studied in male rats using in vivo microdialysis. Results: Releasing of dopamine increased significantly in the nucleus accumbens when a receptive female was introduced behind a screen (p 0.001). During copulation, dopamine increased markedly in control and treated rats. Phoenix dactylifera Date Palm Pollen enhanced the orientation of males towards females by increasing mounting and ano-genital investigatory behavior. Improving of sexual behavior and dopamine release was higher in treated rats in comparison with control (p 0.001). Conclusion: These results indicate a neurochemical basis for interaction between dopaminergic agents and male sexual behavior. Therefore, Phoenix dactylifera Date Palm Pollen seems to act as a dopamine agonist and to cure male infertility. It can be used as an aphrodisiac that leads to further increases in dopamine release. 展开更多
关键词 PHOENIX dactylifera Date Palm POLLEN Dopamine Nucleus ACCUMBENS MICRODIALYSIS male Rat
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Early infant male circumcision:Systematic review,risk-benefit analysis,and progress in policy 被引量:2
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作者 Brian J Morris Sean E Kennedy +6 位作者 Alex D Wodak Adrian Mindel David Golovsky Leslie Schrieber Eugenie R Lumbers David J Handelsman John B Ziegler 《World Journal of Clinical Pediatrics》 2017年第1期89-102,共14页
AIM To determine whether recent evidence-based United States polices on male circumcision(MC) apply to comparable Anglophone countries,Australia and New Zealand.METHODS Articles in 2005 through 2015 were retrieved fro... AIM To determine whether recent evidence-based United States polices on male circumcision(MC) apply to comparable Anglophone countries,Australia and New Zealand.METHODS Articles in 2005 through 2015 were retrieved from PubM ed using the keyword "circumcision" together with 36 relevant subtopics.A further PubM ed search was performed for articles published in 2016.Searches of the EMBASE and Cochrane databases did not yield additional citable articles.Articles were assessed for quality and those rated 2+ and above according to the Scottish Intercollegiate Grading System were studied further.The most relevant andrepresentative of the topic were included.Bibliographies were examined to retrieve further key references.Randomized controlled trials,recent high quality systematic reviews or meta-analyses(level 1++ or 1+ evidence) were prioritized for inclusion.A risk-benefit analysis of articles rated for quality was performed.For efficiency and reliability,recent randomized controlled trials,metaanalyses,high quality systematic reviews and large welldesigned studies were used if available.Internet searches were conducted for other relevant information,including policies and Australian data on claims under Medicare for MC.RESULTS Evidence-based policy statements by the American Academy of Pediatrics(AAP) and the Centers for Disease Control and Prevention(CDC) support infant and later age male circumcision(MC) as a desirable public health measure.Our systematic review of relevant literature over the past decade yielded 140 journal articles that met our inclusion criteria.Together,these showed that early infant MC confers immediate and lifelong benefits by protecting against urinary tract infections having potential adverse long-term renal effects,phimosis that causes difficult and painful erections and "ballooning" during urination,inflammatory skin conditions,inferior penile hygiene,candidiasis,various sexually transmissible infections in both sexes,genital ulcers,and penile,prostate and cervical cancer.Our risk-benefit analysis showed that benefits exceeded procedural risks,which are predominantly minor,by up to 200 to 1.We estimated that more than 1 in 2 uncircumcised males will experience an adverse foreskin-related medical condition over their lifetime.Wide-ranging evidence from surveys,physiological measurements,and the anatomical location of penile sensory receptors responsible for sexual sensation strongly and consistently suggested that MC has no detrimental effect on sexual function,sensitivity or pleasure.United States studies showed that early infant MC is cost saving.The evidence supporting early infant MC has further strengthened since the positive AAP and CDC reviews.CONCLUSION Affirmative MC policies are needed in Australia and New Zealand.Routine provision of accurate,unbiased education,and access in public hospitals,will maximize health and financial benefits. 展开更多
关键词 male CIRCUMCISION EVIDENCE-BASED POLICY Infants Adults Urinary tract INFECTIONS Adverse events Sexually transmitted INFECTIONS GENITAL cancers Riskbenefit analysis COST-BENEFIT
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Effect of apigenin on the reproductive system in male mice 被引量:1
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作者 Hui Li Hong-Bo Li +3 位作者 Ming Zhang Fang Yan Zhong-Xian Zhang Zhi-Lan Li 《Health》 2010年第5期435-440,共6页
This study aimed to characterize the effect of apigenin on the reproductive system in male mice. Adult male mice were treated with intraperitoneal injection of apigenin at the dose levels of 5, 10, 15, 20 and 25 mg/kg... This study aimed to characterize the effect of apigenin on the reproductive system in male mice. Adult male mice were treated with intraperitoneal injection of apigenin at the dose levels of 5, 10, 15, 20 and 25 mg/kg.bw, 0.05% DMSO and 0.9% normal saline daily for seven days. Then, testis and epididymis sperms in sperm motility, sperm morphology, the percentages of ploidy cells and seminiferous epithelium cells at the cell-circle phase, and the ratio of ploidy cells were evaluated. The results showed that sperm density significantly reduced in the 25 mg/kg group compared with the solvent control group. The abnormal sperms were mainly amorphous;non-hook sperms took the second largest group;and banana, double-tail and folded-tail sperms were rare. Abnormal sperms were mainly in the head sperm. Moreover, after intraperitoneal injection of 5 mg/kg apigenin, the percentage of 1C population increased, and the percentage of 4C declined, leading to a significant increase of the 1C:4C ratio, compared with the solvent and negative control groups. The percentage of seminiferous epithelium cells at the cell-circle phase of G0/G1 exhibited a significant increase in the 25 mg/kg group compared with the control groups. Taken together, that apigenin has adverse effects on the reproductive system in adult male mice is demonstrated. 展开更多
关键词 APIGENIN male Mice INTRAPERITONEAL Injection SPERM MOTILITY SPERM Morphology Flow CYTOMETRY
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Role of Estrogens on Human Male Reproductive System
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作者 Vincenzo Rochira Lucia Zirilli +3 位作者 Bruno Madeo Antonio Cesare Carani Antonio R. M. Granata 《Journal of Reproduction and Contraception》 CAS 2004年第2期65-80,共16页
This review focuses on estrogen role on human male physiology. Biological estrogen actions on male reproductive system are summarized with particular regard to the effects of congenital estrogen deprivation in men. ... This review focuses on estrogen role on human male physiology. Biological estrogen actions on male reproductive system are summarized with particular regard to the effects of congenital estrogen deprivation in men. The effects of estrogen on spermatogenesis, hormonal secretion and gonadotropin feedback and on sexual behavior are discussed. It is remarked that the role of estrogens in male reproduction is a very recent acquisition in reproductive endocrinology, but it promises new future fields of research to be investigated as well as the possible disclosure of new strategies in clinical practice. 展开更多
关键词 ESTROGEN male sexual behaviour male infertility reproductive system gonadotropin feedback
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Pro-fertility effect of Ficus carica fruit extract in streptozotocin-induced male rats 被引量:1
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作者 Mat Noor Mahanem Subramaniam Puvaratnesh +1 位作者 AbuBakar Umarqayum Shamsusah Nadia A 《Asian pacific Journal of Reproduction》 CAS 2024年第1期34-41,共8页
Objective:To explore the impact of Ficus carica fruit aqueous extract on fertility parameters in streptozotocin(STZ)-induced male rats.Methods:Twenty-four male Sprague-Dawley rats were divided into four different grou... Objective:To explore the impact of Ficus carica fruit aqueous extract on fertility parameters in streptozotocin(STZ)-induced male rats.Methods:Twenty-four male Sprague-Dawley rats were divided into four different groups.All groups except a normal control group were induced with 50 mg/kg of streptozotocin(STZ)intravenously to induce diabetes.A positive control group was treated with an antidiabetic drug,metformin(500 mg/kg)whereas a negative control group remained untreated throughout the experiment.Meanwhile,another diabetic rat group received treatment with 400 mg/kg of aqueous Ficus carica fruit extract.Rats in the treatment group were administered Ficus carica fruit aqueous extract daily through forcefeeding via oral gavage for a 21-day period.Assessments included the sperm quality(count,motility and morphology),histology of the testes,serum testosterone and fasting blood glucose(FBG)level.Results:The FBG level of the Ficus carica-treated rats exhibited a significant decrease compared to the negative control group(P<0.05).Sperm quality analysis also indicated that the aqueous Ficus carica extract had significant positive effects on sperm count and motility(P<0.05).The histology of the testes in Ficus caricatreated rats revealed an improved cell arrangement in the germinal cell layer.Furthermore,serum testosterone level showed an increment in the Ficus carica treatment group in comparison to the negative control group.Conclusions:Our findings provide compelling evidence for the profertility and anti-hyperglycemic properties of aqueous Ficus carica fruit extract in diabetic-induced male rats. 展开更多
关键词 Ficus carica fruit male fertility parameter Diabetes mellitus Anti-hyperglicemic
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Effects of Medicines and Supplements on Spontaneous Pregnancy and Semen Parameters in Male Infertility:A Systematic Review Update and Network Meta-Analysis
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作者 Jian Li Qi Wu +3 位作者 Ernest Hung Yu Ng Ben Willem J.Mol Xiao Ke Wu Chi Chiu Wang 《Engineering》 SCIE EI CAS 2022年第9期198-209,共12页
In this study,we used a network meta-analysis(NMA)to compare the effectiveness of medicines and supplements for idiopathic male infertility and to identify the best treatment.Medline,Excerpta Medica Database(EMBASE),O... In this study,we used a network meta-analysis(NMA)to compare the effectiveness of medicines and supplements for idiopathic male infertility and to identify the best treatment.Medline,Excerpta Medica Database(EMBASE),Ovid,and China National Knowledge Infrastructure(CNKI),were searched for the period from January 1990 to June 2021 using the keywords"male infertility,""medical therapy,""supplement/nutrient therapy,"and related terms.Randomized controlled trials(RCTs)investigating medicines(mainly follicle-stimulating hormone(FSH),androgen,and clomiphene/tamoxifen)or supplements(mainly zinc,selenium,vitamin C or E,carnitine,coenzyme Q10(CoQ10),or combined treatment)for idiopathic infertile men were selected for meta-analysis.Preferred reporting items for systematic reviews and meta-analysis(PRISMA)was used for data extraction,and a risk-of-bias tool and grades of recommendation,assessment,development,and evaluation(GRADE)system adapted to the NMA were employed to assess the quality of the evidence.The primary outcomes were live birth and spontaneous pregnancy rate(SPR).The secondary outcomes were sperm parameters(including concentration,progressive motility,and morphology)and side effects.In total,65 RCTs involving 7541 men with sperm abnormalities but normal hormone levels were included.A total of 36 studies reported SPR but only three reported live birth rates.The quality of the included studies was found to be moderate to high.Compared with a placebo or being untreated,carnitine plus vitamins significantly improved SPR(relative risk(RR)=3.7,95%confidence interval(95%CI),1.6-8.5);fatty acids significantly increased sperm concentrations(mean difference(MD)=12.5×10^(6)mL-1,95%CI,3.1×10^(6)-22.0×10^(6));and selective estrogen receptor modulator(SERM)plus CoQ10 significantly improved sperm progressive motility(MD=11.0%,95%CI,0.1%-21.9%)and normal sperm morphology(MD=11.0%,95%CI,4.6%-17.4%).The most optimal intervention was carnitine plus vitamins and fatty acids for SPR and sperm concentrations,respectively,even after excluding trials at a high risk of bias.Compared with a placebo or being untreated,FSH(RR=4.9,95%CI,1.1-21.3)significantly increased SPR,whereas SERM plus kallikrein increased sperm concentration(MD=16.5×10^(6)mL^(-1),95%CI,1.6×10^(6)-31.4×10^(6)),and SERM plus CoQ10 significantly improved sperm progressive motility(MD=11.3%,95%CI,7.3%-15.4%)and normal morphology(MD=11.2%,95%CI,5.4%-16.9%)in men with oligoasthenozoospermia(OA).In terms of side effects,fatty acids and pentoxifylline were associated with foul breath and/or a bad taste(RR=8.1,95%CI,1.0-63.5)and vomiting(RR=8.0,95%CI,1.0-63.0),respectively.In conclusion,the optimal treatment for male infertility for live birth is still unknown.Carnitine plus vitamins and FSH are likely to be better than other therapies in achieving successful spontaneous pregnancy in couples overall and in cou ples with men with OA,respectively.The efficacy of other treatments on pregnancy outcomes warrants further verification. 展开更多
关键词 male infertility MEDICINE SUPPLEMENT Spontaneous pregnancy rate Sperm parameters
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Development of Insect-Resistant Hybrid Rice by Introgressing the Bt Gene from Bt Rice Huahui 1 into Ⅱ-32A/B,a Widely Used Cytogenic Male Sterile System
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作者 LAI Yun-song HUANG Hai-qing +4 位作者 XU Meng-yun WANG Liang-chao ZHANG Xiao-bo ZHANG Ji-wen TU Ju-min 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2014年第10期2081-2090,共10页
Huahui 1 is an elite transgenic male sterile restorer line of wild rice abortive-type that expresses a Bacillus thuringiensis (Bt) δ-endotoxin and provides effective and economic control oflepidopteran insects. To ... Huahui 1 is an elite transgenic male sterile restorer line of wild rice abortive-type that expresses a Bacillus thuringiensis (Bt) δ-endotoxin and provides effective and economic control oflepidopteran insects. To exploit Huahui 1 to develop a new Bt rice, the insertion site of the Bt gene was determined by thermal asymmetric interlaced PCR (TAIL-PCR). Bt was located in the promoter region ofLOC. Os10g10360, approximately 5.35 Mb from the telomere of the short arm of chromosome 10. For the first time, a Bt cytoplasmic male sterile (CMS) system was developed by introgressing Bt from Huahui 1. The recipient CMS system used consisted of Indonesia paddy rice-type II-32B (maintainer line) and II-32A (male sterile line). Marker-assisted selection was used to increase selection efficiency in the backcrossing program. In BC5F1, the Bt plant 85015-8 was selected for further analyses, as it had the highest SSR marker homozygosity. In addition, the linkage drag of the foreign Bt gene in 85015-8 was minimized to 8.01-11.46 Mb. The foreign Bt gene was then delivered from 85015-8 into II-32A. The resultant Bt II-32A and Bt II-32B lines were both resistant to lepidopteran in field trials, and agronomic traits were not disturbed. The maintainability of II-32B, and the male sterility and general combining ability of II-32A, were not affected by the Bt introgression. This study demonstrates a simple and fast approach to develop Bt hybrid rice. 展开更多
关键词 Bt rice male sterile line genome walker marker-assisted backcross (MAB) marker-assisted selection (MAS) SSR
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No Male Preference for Large Females in the Asian Common Toad(Duttaphrynus melanostictus):Effect of the Sex Ratio and Breeding System
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作者 Lixia ZHANG Xiangyu YUAN +4 位作者 Yongsun SHENG Xueting ZHONG Jiahong LIAO Zhenhao LIU Wei CHEN 《Asian Herpetological Research》 SCIE CSCD 2020年第4期328-334,共7页
Mating preferences are common in natural populations of animals.Numerous studies have shown that male mate choice can occur in a wide range of taxa.However,male mating preferences are still poorly understood in anuran... Mating preferences are common in natural populations of animals.Numerous studies have shown that male mate choice can occur in a wide range of taxa.However,male mating preferences are still poorly understood in anurans.Sexual selection theory predicts that male mate choice is not expected to arise if 1) adult population exhibited a highly male-biased sex ratio which will diminish male mating success;2) males provide less parental care;3) mating success of males is associated with chorus tenure in which males would maximize their fitness by mating with multiple mates.We tested these predictions in the Asian common toad Duttaphrynus melanostictus from southeastern Tibet,China.Our field experimental results indicated that,the breeding population exhibited a highly male-biased sex ratio,called males did not defend sites which contain significant resources required by females and offspring,both sexes provided no parental care after egg-laying,and the toad species was characterized with prolonged breeding season chorus attendance.In male mate choice experiment,males did not show preferences for a larger gravid female over a smaller gravid female.We suggest that male mating success in the Asian common toad is likely determined by the number but not the quality of mates.Future research should focus on how sexual selection on male acoustic signaling and how female preference exert different types of selection pressure on male call traits in this Tibet toad. 展开更多
关键词 Asian common toad chorus attendance Duttaphrynus melanostictus male mating preference parental care sex ratio
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Dose-dependent effects of pentabrominated diphenyl ethers on sexual hormone and histology of male reproductive system in rats
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作者 Lingyan Yuan Meiqing Fan Ping Zhan 《Journal of Biomedical Science and Engineering》 2012年第6期302-306,共5页
Polybrominated diphenyl ethers (PBDEs) used as flame retardants are widely used in a variety of consumer products, like textiles, foam and building materials. They are persistent in the environment, and increasing lev... Polybrominated diphenyl ethers (PBDEs) used as flame retardants are widely used in a variety of consumer products, like textiles, foam and building materials. They are persistent in the environment, and increasing levels of PBDEs have been found both biota and human tissue samples. In the present study we examined the dose-dependent effects of pentabrominated diphenyl ether (PBDE-99) on the serum hormone and histology of reproductive system in male rats. 40 health 4-week-old SD rats were divided into 4 groups randomly. The treated rats were orally administered with BDE-99 at the dose of 60,120 or 180 mg/kg, while controls received equal volume of corn oil. We examined the changes of serum hormone levels, histopathology, and PCNA after 30 days exposure to BDE-99. With increasing doses of BDE-99, testis organ coefficient increased, serum hormone levels lower, PCNA positive expression reduced, and the degeneration of seminiferous epithelium were revealed in treated group. These indicators with significant dose-effect relationship showed that BDE-99 can reduce the serum sex hormone levels and cause severe degeneration of seminiferous epithelium. 展开更多
关键词 Pentabrominated DIPHENYL ETHERS male REPRODUCTIVE system the Relationship of DOSE-EFFECT
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CCDC181 is required for sperm flagellum biogenesis and male fertility in mice
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作者 Xiang-Jun Zhang Xiao-Ning Hou +7 位作者 Jian-Teng Zhou Bao-Lu Shi Jing-Wei Ye Meng-Lei Yang Xiao-Hua Jiang Bo Xu Li-Min Wu Qing-Hua Shi 《Zoological Research》 SCIE CSCD 2024年第5期1061-1072,共12页
The structural integrity of the sperm flagellum is essential for proper sperm function.Flagellar defects can result in male infertility,yet the precise mechanisms underlying this relationship are not fully understood.... The structural integrity of the sperm flagellum is essential for proper sperm function.Flagellar defects can result in male infertility,yet the precise mechanisms underlying this relationship are not fully understood.CCDC181,a coiled-coil domain-containing protein,is known to localize on sperm flagella and at the basal regions of motile cilia.Despite this knowledge,the specific functions of CCDC181 in flagellum biogenesis remain unclear.In this study,Ccdc181 knockout mice were generated.The absence of CCDC181 led to defective sperm head shaping and flagellum formation.Furthermore,the Ccdc181 knockout mice exhibited extremely low sperm counts,grossly aberrant sperm morphologies,markedly diminished sperm motility,and typical multiple morphological abnormalities of the flagella(MMAF).Additionally,an interaction between CCDC181 and the MMAF-related protein LRRC46 was identified,with CCDC181 regulating the localization of LRRC46 within sperm flagella.These findings suggest that CCDC181 plays a crucial role in both manchette formation and sperm flagellum biogenesis. 展开更多
关键词 male infertility CCDC181 MMAF SPERMIOGENESIS Flagellum biogenesis
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Advances in DNA methylation and its role in cytoplasmic male sterility in higher plants
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作者 Atiqur Rahman Hasan Sofiur Rahman +9 位作者 Shakil Uddin Naima Sultana Shirin Akhter Ujjal Kumar Nath Shamsun Nahar Begum Mazadul Islam Afroz Naznin Nurul Amin Sharif Ahmed Akbar Hossain 《Journal of Integrative Agriculture》 SCIE CSCD 2024年第1期1-19,共19页
The impact of epigenetic modifications like DNA methylation on plant phenotypes has expanded the possibilities for crop development.DNA methylation plays a part in the regulation of both the chromatin structure and ge... The impact of epigenetic modifications like DNA methylation on plant phenotypes has expanded the possibilities for crop development.DNA methylation plays a part in the regulation of both the chromatin structure and gene expression,and the enzyme involved,DNA methyltransferase,executes the methylation process within the plant genome.By regulating crucial biological pathways,epigenetic changes actively contribute to the creation of the phenotype.Therefore,epigenome editing may assist in overcoming some of the drawbacks of genome editing,which can have minor off-target consequences and merely facilitate the loss of a gene’s function.These drawbacks include gene knockout,which can have such off-target effects.This review provides examples of several molecular characteristics of DNA methylation,as well as some plant physiological processes that are impacted by these epigenetic changes in the plants.We also discuss how DNA alterations might be used to improve crops and meet the demands of sustainable and environmentally-friendly farming. 展开更多
关键词 DNA methylation EPIGENETICS CMS male sterility chromatin architecture gene expression higher plants
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Exploring the relationship between Hashimoto's thyroiditis and male fertility:A meta-analytic and meta-regression perspective on hormonal and seminal factors
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作者 Seyed Sobhan Bahreiny Mojtaba Aghaei +1 位作者 Mahdi Amraei Mohammad Reza Dabbagh 《Asian pacific Journal of Reproduction》 CAS 2024年第4期147-159,共13页
Objective:To explore the relationship between Hashimoto's autoimmune hypothyroidism(HT)and male fertility,focusing on hormonal and seminal factors.Methods:A systematic literature search was conducted across databa... Objective:To explore the relationship between Hashimoto's autoimmune hypothyroidism(HT)and male fertility,focusing on hormonal and seminal factors.Methods:A systematic literature search was conducted across databases such as PubMed,Web of Science,EMBASE,Scopus,Cochrane,and Google Scholar,covering studies published from January 2000 to March 2024.Studies investigating the impact of HT on semen quality parameters and reproductive hormones were included.Pooled effect estimates were calculated using standard mean difference(SMD)and 95%confidence intervals(CI).Results:A total of 8 studies with 8965 participants were included.HT significantly affected semen quality and reproductive hormone levels.Specifically,there was a notable decrease in progressive morphology(SMD=-0.78;95%CI:-1.40 to-0.17;P=0.01)and sperm motility(SMD=-1.151;95%CI:-1.876 to-0.425;P=0.002).In addition,there were no significant changes in reproductive hormones,although there were elevated levels of luteinizing hormone(SMD=0.437;95%CI:0.000 to 0.874;P=0.050)and follicle-stimulating hormone(SMD=0.293;95%CI:-0.171 to 0.758;P=0.216),with a slight impact on testosterone levels(SMD=-1.143;95%CI:-2.487 to 0.200;P=0.095).Conclusions:This systematic review and meta-analysis provides robust evidence of the detrimental effects of HT on semen quality and reproductive hormones,underscoring the necessity for thorough evaluation and management of thyroid function in male infertility assessments. 展开更多
关键词 Hashimoto thyroiditis male fertility Semen quality Reproductive hormones META-ANALYSIS META-REGRESSION
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Granulomatous mastitis in a 50-year-old male:A case report and review of literature
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作者 Le-Yin Cui Chen-Ping Sun +1 位作者 Yun-Yuan Li Sheng Liu 《World Journal of Clinical Cases》 SCIE 2024年第2期451-459,共9页
BACKGROUND Granulomatous mastitis(GM)an inflammatory disease of the breast that usually affects women of childbearing age,occurs very rarely in males.CASE SUMMARY We present a case study of a 50-year-old male patient ... BACKGROUND Granulomatous mastitis(GM)an inflammatory disease of the breast that usually affects women of childbearing age,occurs very rarely in males.CASE SUMMARY We present a case study of a 50-year-old male patient with GM.The patient developed a breast lump following the cleaning of a previously embedded dirtfilled nipple.While an initial improvement was noted with antibiotic therapy,a recurrence occurred a year later,showing resistance to the previously effective antibiotics.Subsequently,the lesion was excised.The histopathological examination confirmed the diagnosis of GM.CONCLUSION GM should be considered a possible diagnosis of male breast masses. 展开更多
关键词 Granulomatous mastitis male PATHOLOGY Differential diagnosis Case report
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Morphological and cytological assessments reveal pollen degradation causes pollen abortion in cotton cytoplasmic male sterility lines
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作者 PEI Qingyu LIU Jinshan +10 位作者 GUO Chunping MA Xiaomei LIU Xiaoyan YOU Chunyuan LIN Hairong LI Zhibo ZHAO Ruihai ZHU Bo WU Yuanlong PAN Zhenyuan NIE Xinhui 《Journal of Cotton Research》 CAS 2024年第3期266-276,共11页
Background Understanding the mechanism of male sterility is crucial for producing hybrid seeds and developing sterile germplasm resources.However,only a few cytoplasmic male sterility(CMS)lines of cotton have been pro... Background Understanding the mechanism of male sterility is crucial for producing hybrid seeds and developing sterile germplasm resources.However,only a few cytoplasmic male sterility(CMS)lines of cotton have been produced due to several challenges,like inadequate variation of agronomic traits,incomplete sterility,weak resilience of restorer lines,and difficulty in combining strong dominance.Therefore,the morphological and cytological identification of CMS in cotton will facilitate hybrid breeding.Results Two F_(2) segregating populations of cotton were constructed from cytoplasmic male sterile lines(HaA and 01A,maternal)and restorer lines(HaR and 26R,paternal).Genetic analysis of these populations revealed a segregation ratio of 3:1 for fertile to sterile plants.Phenotypic analysis indicated no significant differences in traits of flower bud development between sterile and fertile plants.However,sterile plants exhibited smaller floral organs,shortened filament lengths,and anther atrophy on the flowering day in comparison with the fertile plants.When performed scanning electron microscopy(SEM),the two F_(2) populations revealed morphological variations in the anther epidermis.Cellular analysis showed no significant differences in pollen development before pollen maturation.Interestingly,between the pollen maturation and flowering stages,the tapetum layer of sterile plants degenerated prematurely,resulting in abnormal pollen grains and gradual pollen degradation.Conclusion The results of this study suggest that fertility-restoring genes are controlled by a single dominant gene.Sterile plants exhibit distinctive floral morphology,which is characterized by stamen atrophy and abnormal anthers.Pollen abortion occurs between pollen maturity and flowering,indicating that premature tapetum degradation may be the primary cause of pollen abortion.Overall,our study provides a theoretical basis for utilizing CMS in hybrid breeding and in-depth investigation of the dominant configuration of cotton hybrid combinations,mechanisms of sterility,and the role of sterile and restorer genes. 展开更多
关键词 COTTON Cytoplasmic male sterility Genetic analysis Morphological characteristics Pollen development
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Evaluation of the Pharmacological Potential of Strychnos camptoneura Trunk Bark Hydroethanol Extract on Reproductive Functions in Male Guinea Pigs (Cavia porcellus) Exposed to Cypermethrin
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作者 Akassa Herman Moukouma Constantin +5 位作者 Diakouka Diambalou Rancia Colombe Njilo Tchatchouang Didier Gestil Mouzita Judicaël Ferry Mpika M’boko Etou Ossibi Arnaud Wilfrid Abena Ange Antoine 《American Journal of Plant Sciences》 CAS 2024年第10期884-898,共15页
The main objective of this study was to evaluate the pharmacological potential of the hydroethanol extract of Strychnos camptoneura trunk bark on reproductive functions in male guinea pigs exposed to cypermethrin. The... The main objective of this study was to evaluate the pharmacological potential of the hydroethanol extract of Strychnos camptoneura trunk bark on reproductive functions in male guinea pigs exposed to cypermethrin. The results showed that administration of the hydroethanolic extract (100 and 250 mg/kg) of Strychnos camptoneura trunk bark after exposure of the animals to cypermethrin induced a highly significant (p Strychnos camptoneura trunk bark produced a highly significant (p Strychnos camptoneura trunk bark may have protective effects against cypermethrin-induced male infertility due to its androgenic, spermatogenic and antiradical properties. 展开更多
关键词 Strychnos Camptoneura Guinea Pig males CYPERMETHRIN Reproductive Function
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