A key aspect in extracting quantitative information from FMI logs is to segment the FMI image to get images of pores, vugs and fractures. A segmentation method based on the dyadic wavelet transform in 2-D is introduce...A key aspect in extracting quantitative information from FMI logs is to segment the FMI image to get images of pores, vugs and fractures. A segmentation method based on the dyadic wavelet transform in 2-D is introduced in this paper. The first step is to find all the edge pixels of the FMI image using the 2-D wavelet transform. The second step is to calculate a segmentation threshold based on the average value of the edge pixels. Field data processing examples show that sub-images of vugs and fractures can be correctly separated from original FMI data continuously and automatically along the depth axis. The image segmentation lays the foundation for in-situ parameter calculation.展开更多
An effective processing method for biomedical images and the Fuzzy C-mean (FCM) algorithm based on the wavelet transform are investigated.By using hierarchical wavelet decomposition, an original image could be decompo...An effective processing method for biomedical images and the Fuzzy C-mean (FCM) algorithm based on the wavelet transform are investigated.By using hierarchical wavelet decomposition, an original image could be decomposed into one lower image and several detail images. The segmentation started at the lowest resolution with the FCM clustering algorithm and the texture feature extracted from various sub-bands. With the improvement of the FCM algorithm, FCM alternation frequency was decreased and the accuracy of segmentation was advanced.展开更多
Direct algorithm of wavelet transform (WT) is the numerical algorithmobtained from the integral formula of WT by directly digitization. Some problems on realizing thealgorithm are studied. Some conclusions on the dire...Direct algorithm of wavelet transform (WT) is the numerical algorithmobtained from the integral formula of WT by directly digitization. Some problems on realizing thealgorithm are studied. Some conclusions on the direct algorithm of discrete wavelet transform (DWT),such as discrete convolution operation formula of wavelet coefficients and wavelet components,sampling principle and technology to wavelets, deciding method for scale range of wavelets, measuresto solve edge effect problem, etc, are obtained. The realization of direct algorithm of continuouswavelet transform (CWT) is also studied. The computing cost of direct algorithm and Mallat algorithmof DWT are still studied, and the computing formulae are obtained. These works are beneficial todeeply understand WT and Mallat algorithm. Examples in the end show that direct algorithm can alsobe applied widely.展开更多
An efficient multi-threshold approach to segment thermal image is given based on wavelet transform. The gray-level histogram of original image is obtained. In order to reduce the effect of noise, the gray-level histog...An efficient multi-threshold approach to segment thermal image is given based on wavelet transform. The gray-level histogram of original image is obtained. In order to reduce the effect of noise, the gray-level histogram is smoothed by Bezier curve and Bezier histogram is obtained. One dimension stationary wavelet transform is done to the curvature curve of Bezier histogram. Positions of peak values of curvature curve in wavelet domain are adjusted from 'fine-to-coarse' at all scales. The gray level values, which are located in adjusted peak values at all scales, are considered as segmentation thresholds. The gray level values of valley between peaks are considered as quantity gray levels. Optimal segmentation scale is obtained by a cost criterion. The results of experiment show that a target can be segmented effectively from complex background in thermal image by new approach.展开更多
A new meaningful image encryption algorithm based on compressive sensing(CS)and integer wavelet transformation(IWT)is proposed in this study.First of all,the initial values of chaotic system are encrypted by RSA algor...A new meaningful image encryption algorithm based on compressive sensing(CS)and integer wavelet transformation(IWT)is proposed in this study.First of all,the initial values of chaotic system are encrypted by RSA algorithm,and then they are open as public keys.To make the chaotic sequence more random,a mathematical model is constructed to improve the random performance.Then,the plain image is compressed and encrypted to obtain the secret image.Secondly,the secret image is inserted with numbers zero to extend its size same to the plain image.After applying IWT to the carrier image and discrete wavelet transformation(DWT)to the inserted image,the secret image is embedded into the carrier image.Finally,a meaningful carrier image embedded with secret plain image can be obtained by inverse IWT.Here,the measurement matrix is built by both chaotic system and Hadamard matrix,which not only retains the characteristics of Hadamard matrix,but also has the property of control and synchronization of chaotic system.Especially,information entropy of the plain image is employed to produce the initial conditions of chaotic system.As a result,the proposed algorithm can resist known-plaintext attack(KPA)and chosen-plaintext attack(CPA).By the help of asymmetric cipher algorithm RSA,no extra transmission is needed in the communication.Experimental simulations show that the normalized correlation(NC)values between the host image and the cipher image are high.That is to say,the proposed encryption algorithm is imperceptible and has good hiding effect.展开更多
Clustering algorithms in feature space are important methods in image segmentation. The choice of the effective feature parameters and the construction of the clustering method are key problems encountered with cluste...Clustering algorithms in feature space are important methods in image segmentation. The choice of the effective feature parameters and the construction of the clustering method are key problems encountered with clustering algorithms. In this paper, the multifractal dimensions are chosen as the segmentation feature parameters which are extracted from original image and wavelet-transformed image. SOM (Self-Organizing Map) network is applied to cluster the segmentation feature parameters. The experiment shows that the performance of the presented algorithm is very good.展开更多
Fast and satisfied medical ultrasound segmentation is known to be difficult due to speckle noises and other artificial effects. Since speckle noise is formed from random signals which are emitted by an ultrasound syst...Fast and satisfied medical ultrasound segmentation is known to be difficult due to speckle noises and other artificial effects. Since speckle noise is formed from random signals which are emitted by an ultrasound system, we can’t encounter the same way as other image noises. Lack of information in ultrasound images is another problem. Thus, segmentation results may not be accurate enough by means of customary image segmentation methods. Those methods that can specify undesirable effects and segment them by eliminating artificial effects, should be chosen. It seems to be a complicated work with high computational load. The current study presents a different approach to ultrasound image segmentation that relies mainly on local evaluation, named as local histogram range image method which is modified by means of discrete wavelet transform. Thus, a significant decrease in computational load is then achieved. The results show that it is possible for tissues to be segmented correctly.展开更多
Lots of noises and heterogeneous objects with various sizes coexist in a complex image,such as an ore image;the classical image thresholding method cannot effectively distinguish between ores.To segment ore objects wi...Lots of noises and heterogeneous objects with various sizes coexist in a complex image,such as an ore image;the classical image thresholding method cannot effectively distinguish between ores.To segment ore objects with various sizes simultaneously,two adaptive windows in the image were chosen for each pixel;the gray value of windows was calculated by Otsu's threshold method.To extract the object skeleton,the definition principle of distance transformation templates was proposed.The ores linked together in a binary image were separated by distance transformation and gray reconstruction.The seed region of each object was picked up from the local maximum gray region of the reconstruction image.Starting from these seed regions,the watershed method was used to segment ore object effectively.The proposed algorithm marks and segments most objects from complex images precisely.展开更多
An efficient tropical cyclone(TC) cloud image segmentation method is proposed by combining the curvelet transform,the cubic B-Spline curve,and the continuous wavelet transform.In order to enhance the global and loca...An efficient tropical cyclone(TC) cloud image segmentation method is proposed by combining the curvelet transform,the cubic B-Spline curve,and the continuous wavelet transform.In order to enhance the global and local contrast of the original TC cloud image,a second-generation discrete curvelet transform is implemented for the original TC cloud image.Based on our prior work,the low frequency components are enhanced by using an incomplete Beta transform and the genetic algorithm in the curvelet domain. Then the enhanced TC cloud image is used to segment the main body of the TC from the TC cloud image. First,pre-processing is implemented by B-Spline curves to the original TC cloud image to remove unrelated small cloud masses.A region of interest(ROI) which includes the main body of TC can thus be obtained. Second,the gray-level histogram of ROI is obtained.In order to reduce oscillations of the histogram,the gray-level histogram is smoothed by cubic B-Spline curves and the B-Spline histogram is obtained.The one dimensional continuous wavelet transform is employed for the curvature curve of the B-Spline histogram. A new segmentation cost criterion is given by combining threshold,error,and structure similarity.The optimally segmented image can be obtained by the criterion in the continuous wavelet domain.The optimally segmented image is post-processed to obtain the final segmented TC image.The experimental results show that the main body of TC can be effectively segmented from the complex background in the TC cloud image by the proposed algorithm.展开更多
In this paper, a new medical image classification scheme is proposed using selforganizing map (SOM) combined with multiscale technique. It addresses the problem of the handling of edge pixels in the traditional multis...In this paper, a new medical image classification scheme is proposed using selforganizing map (SOM) combined with multiscale technique. It addresses the problem of the handling of edge pixels in the traditional multiscale SOM classifiers. First, to solve the difficulty in manual selection of edge pixels, a multiscale edge detection algorithm based on wavelet transform is proposed. Edge pixels detected are then selected into the training set as a new class and a mu1tiscale SoM classifier is trained using this training set. In this new scheme, the SoM classifier can perform both the classification on the entire image and the edge detection simultaneously. On the other hand, the misclassification of the traditional multiscale SoM classifier in regions near edges is greatly reduced and the correct classification is improved at the same time.展开更多
To preserve the original signal as much as possible and filter random noises as many as possible in image processing,a threshold optimization-based adaptive template filtering algorithm was proposed.Unlike conventiona...To preserve the original signal as much as possible and filter random noises as many as possible in image processing,a threshold optimization-based adaptive template filtering algorithm was proposed.Unlike conventional filters whose template shapes and coefficients were fixed,multi-templates were defined and the right template for each pixel could be matched adaptively based on local image characteristics in the proposed method.The superiority of this method was verified by former results concerning the matching experiment of actual image with the comparison of conventional filtering methods.The adaptive search ability of immune genetic algorithm with the elitist selection and elitist crossover(IGAE) was used to optimize threshold t of the transformation function,and then combined with wavelet transformation to estimate noise variance.Multi-experiments were performed to test the validity of IGAE.The results show that the filtered result of t obtained by IGAE is superior to that of t obtained by other methods,IGAE has a faster convergence speed and a higher computational efficiency compared with the canonical genetic algorithm with the elitism and the immune algorithm with the information entropy and elitism by multi-experiments.展开更多
In recent years,many medical image fusion methods had been exploited to derive useful information from multimodality medical image data,but,not an appropriate fusion algorithm for anatomical and functional medical ima...In recent years,many medical image fusion methods had been exploited to derive useful information from multimodality medical image data,but,not an appropriate fusion algorithm for anatomical and functional medical images.In this paper,the traditional method of wavelet fusion is improved and a new fusion algorithm of anatomical and functional medical images,in which high-frequency and low-frequency coefficients are studied respectively.When choosing high-frequency coefficients,the global gradient of each sub-image is calculated to realize adaptive fusion,so that the fused image can reserve the functional information;while choosing the low coefficients is based on the analysis of the neighborbood region energy,so that the fused image can reserve the anatomical image's edge and texture feature.Experimental results and the quality evaluation parameters show that the improved fusion algorithm can enhance the edge and texture feature and retain the function information and anatomical information effectively.展开更多
Through research for image compression based on wavelet analysis in recent years, we put forward an adaptive wavelet decomposition strategy. Whether sub-images are to be decomposed or not are decided by their energy d...Through research for image compression based on wavelet analysis in recent years, we put forward an adaptive wavelet decomposition strategy. Whether sub-images are to be decomposed or not are decided by their energy defined by certain criterion. Then we derive the adaptive wavelet decomposition tree (AWDT) and the way of adjustable compression ratio. According to the feature of AWDT, this paper also deals with the strategies which are used to handle different sub-images in the procedure of quantification and coding of the wavelet coefficients. Through experiments, not only the algorithm in the paper can adapt to various images, but also the quality of recovered image is improved though compression ratio is higher and adjustable. When their compression ratios are near, the quality of subjective vision and PSNR of the algorithm are better than those of JPEG algorithm.展开更多
A novel optimal image watermarking scheme is proposed in which the genetic algorithm (GA) is employed to obtain the improvement of algorithm performance. Arnold transform is utilized to obtain the scrambled watermark,...A novel optimal image watermarking scheme is proposed in which the genetic algorithm (GA) is employed to obtain the improvement of algorithm performance. Arnold transform is utilized to obtain the scrambled watermark, and then the embedding and extraction of watermark are implemented in digital wavelet transform (DWT) domain. During the watermarking process, GA is employed to search optimal parameters of embedding strength and times of Arnold transform to gain the optimization of watermarking performance. Simulation results show that the proposed method can improve the quality of watermarked image and give almost the same robustness of the watermark.展开更多
Aim To fuse the fluorescence image and transmission image of a cell into a single image containing more information than any of the individual image. Methods Image fusion technology was applied to biological cell imag...Aim To fuse the fluorescence image and transmission image of a cell into a single image containing more information than any of the individual image. Methods Image fusion technology was applied to biological cell imaging processing. It could match the images and improve the confidence and spatial resolution of the images. Using two algorithms, double thresholds algorithm and denoising algorithm based on wavelet transform,the fluorescence image and transmission image of a Cell were merged into a composite image. Results and Conclusion The position of fluorescence and the structure of cell can be displyed in the composite image. The signal-to-noise ratio of the exultant image is improved to a large extent. The algorithms are not only useful to investigate the fluorescence and transmission images, but also suitable to observing two or more fluoascent label proes in a single cell.展开更多
基金The research was supported by the FifteenthNational Scientific and Technological Project (2001-BA605A-03-02)
文摘A key aspect in extracting quantitative information from FMI logs is to segment the FMI image to get images of pores, vugs and fractures. A segmentation method based on the dyadic wavelet transform in 2-D is introduced in this paper. The first step is to find all the edge pixels of the FMI image using the 2-D wavelet transform. The second step is to calculate a segmentation threshold based on the average value of the edge pixels. Field data processing examples show that sub-images of vugs and fractures can be correctly separated from original FMI data continuously and automatically along the depth axis. The image segmentation lays the foundation for in-situ parameter calculation.
文摘An effective processing method for biomedical images and the Fuzzy C-mean (FCM) algorithm based on the wavelet transform are investigated.By using hierarchical wavelet decomposition, an original image could be decomposed into one lower image and several detail images. The segmentation started at the lowest resolution with the FCM clustering algorithm and the texture feature extracted from various sub-bands. With the improvement of the FCM algorithm, FCM alternation frequency was decreased and the accuracy of segmentation was advanced.
基金This project is supported by National Natural Science Foundation of China (No.50135050)
文摘Direct algorithm of wavelet transform (WT) is the numerical algorithmobtained from the integral formula of WT by directly digitization. Some problems on realizing thealgorithm are studied. Some conclusions on the direct algorithm of discrete wavelet transform (DWT),such as discrete convolution operation formula of wavelet coefficients and wavelet components,sampling principle and technology to wavelets, deciding method for scale range of wavelets, measuresto solve edge effect problem, etc, are obtained. The realization of direct algorithm of continuouswavelet transform (CWT) is also studied. The computing cost of direct algorithm and Mallat algorithmof DWT are still studied, and the computing formulae are obtained. These works are beneficial todeeply understand WT and Mallat algorithm. Examples in the end show that direct algorithm can alsobe applied widely.
文摘An efficient multi-threshold approach to segment thermal image is given based on wavelet transform. The gray-level histogram of original image is obtained. In order to reduce the effect of noise, the gray-level histogram is smoothed by Bezier curve and Bezier histogram is obtained. One dimension stationary wavelet transform is done to the curvature curve of Bezier histogram. Positions of peak values of curvature curve in wavelet domain are adjusted from 'fine-to-coarse' at all scales. The gray level values, which are located in adjusted peak values at all scales, are considered as segmentation thresholds. The gray level values of valley between peaks are considered as quantity gray levels. Optimal segmentation scale is obtained by a cost criterion. The results of experiment show that a target can be segmented effectively from complex background in thermal image by new approach.
基金This work was supported in part by the National Natural Science Foundation of China(Grant Nos.61972103,61772371,62172301)the Natural Science Foundation of Guangdong Province of China(2019A1515011361)+2 种基金the Fundamental Research Funds for the Central Universities of China(22120210545)the Key Scientific Research Project of Education Department of Guangdong Province of China(2020ZDZX3064)the Postgraduate Education Innovation Project of Guangdong Ocean University of China(202143).
文摘A new meaningful image encryption algorithm based on compressive sensing(CS)and integer wavelet transformation(IWT)is proposed in this study.First of all,the initial values of chaotic system are encrypted by RSA algorithm,and then they are open as public keys.To make the chaotic sequence more random,a mathematical model is constructed to improve the random performance.Then,the plain image is compressed and encrypted to obtain the secret image.Secondly,the secret image is inserted with numbers zero to extend its size same to the plain image.After applying IWT to the carrier image and discrete wavelet transformation(DWT)to the inserted image,the secret image is embedded into the carrier image.Finally,a meaningful carrier image embedded with secret plain image can be obtained by inverse IWT.Here,the measurement matrix is built by both chaotic system and Hadamard matrix,which not only retains the characteristics of Hadamard matrix,but also has the property of control and synchronization of chaotic system.Especially,information entropy of the plain image is employed to produce the initial conditions of chaotic system.As a result,the proposed algorithm can resist known-plaintext attack(KPA)and chosen-plaintext attack(CPA).By the help of asymmetric cipher algorithm RSA,no extra transmission is needed in the communication.Experimental simulations show that the normalized correlation(NC)values between the host image and the cipher image are high.That is to say,the proposed encryption algorithm is imperceptible and has good hiding effect.
文摘Clustering algorithms in feature space are important methods in image segmentation. The choice of the effective feature parameters and the construction of the clustering method are key problems encountered with clustering algorithms. In this paper, the multifractal dimensions are chosen as the segmentation feature parameters which are extracted from original image and wavelet-transformed image. SOM (Self-Organizing Map) network is applied to cluster the segmentation feature parameters. The experiment shows that the performance of the presented algorithm is very good.
文摘Fast and satisfied medical ultrasound segmentation is known to be difficult due to speckle noises and other artificial effects. Since speckle noise is formed from random signals which are emitted by an ultrasound system, we can’t encounter the same way as other image noises. Lack of information in ultrasound images is another problem. Thus, segmentation results may not be accurate enough by means of customary image segmentation methods. Those methods that can specify undesirable effects and segment them by eliminating artificial effects, should be chosen. It seems to be a complicated work with high computational load. The current study presents a different approach to ultrasound image segmentation that relies mainly on local evaluation, named as local histogram range image method which is modified by means of discrete wavelet transform. Thus, a significant decrease in computational load is then achieved. The results show that it is possible for tissues to be segmented correctly.
基金supported by the National Key Technologies R & D Program of China (No.2009BAB48B02)the National High-Tech Research and Development Program of China (Nos.2010AA060278600 and 2008AA062101)
文摘Lots of noises and heterogeneous objects with various sizes coexist in a complex image,such as an ore image;the classical image thresholding method cannot effectively distinguish between ores.To segment ore objects with various sizes simultaneously,two adaptive windows in the image were chosen for each pixel;the gray value of windows was calculated by Otsu's threshold method.To extract the object skeleton,the definition principle of distance transformation templates was proposed.The ores linked together in a binary image were separated by distance transformation and gray reconstruction.The seed region of each object was picked up from the local maximum gray region of the reconstruction image.Starting from these seed regions,the watershed method was used to segment ore object effectively.The proposed algorithm marks and segments most objects from complex images precisely.
基金Supported by the National Natural Science Foundation of China(40805048)Zhejiang Provincial Natural Science Foundation (Y506203)+2 种基金Shanghai Typhoon Institute/China Meteorological Administration(2008ST01)the State Key Laboratory of Severe Weather/Chinese Academy of Meteorological Sciences(2008LASW-B03)the Research Foundation of State Key Laboratory of Remote Sensing Science jointly sponsored by the Institute of Remote Sensing Applications of Chinese Academy of Sciences and Beijing Normal University(2009KFJJ013)
文摘An efficient tropical cyclone(TC) cloud image segmentation method is proposed by combining the curvelet transform,the cubic B-Spline curve,and the continuous wavelet transform.In order to enhance the global and local contrast of the original TC cloud image,a second-generation discrete curvelet transform is implemented for the original TC cloud image.Based on our prior work,the low frequency components are enhanced by using an incomplete Beta transform and the genetic algorithm in the curvelet domain. Then the enhanced TC cloud image is used to segment the main body of the TC from the TC cloud image. First,pre-processing is implemented by B-Spline curves to the original TC cloud image to remove unrelated small cloud masses.A region of interest(ROI) which includes the main body of TC can thus be obtained. Second,the gray-level histogram of ROI is obtained.In order to reduce oscillations of the histogram,the gray-level histogram is smoothed by cubic B-Spline curves and the B-Spline histogram is obtained.The one dimensional continuous wavelet transform is employed for the curvature curve of the B-Spline histogram. A new segmentation cost criterion is given by combining threshold,error,and structure similarity.The optimally segmented image can be obtained by the criterion in the continuous wavelet domain.The optimally segmented image is post-processed to obtain the final segmented TC image.The experimental results show that the main body of TC can be effectively segmented from the complex background in the TC cloud image by the proposed algorithm.
文摘In this paper, a new medical image classification scheme is proposed using selforganizing map (SOM) combined with multiscale technique. It addresses the problem of the handling of edge pixels in the traditional multiscale SOM classifiers. First, to solve the difficulty in manual selection of edge pixels, a multiscale edge detection algorithm based on wavelet transform is proposed. Edge pixels detected are then selected into the training set as a new class and a mu1tiscale SoM classifier is trained using this training set. In this new scheme, the SoM classifier can perform both the classification on the entire image and the edge detection simultaneously. On the other hand, the misclassification of the traditional multiscale SoM classifier in regions near edges is greatly reduced and the correct classification is improved at the same time.
基金Project(20040533035) supported by the National Research Foundation for the Doctoral Program of Higher Education of ChinaProject (60874070) supported by the National Natural Science Foundation of China
文摘To preserve the original signal as much as possible and filter random noises as many as possible in image processing,a threshold optimization-based adaptive template filtering algorithm was proposed.Unlike conventional filters whose template shapes and coefficients were fixed,multi-templates were defined and the right template for each pixel could be matched adaptively based on local image characteristics in the proposed method.The superiority of this method was verified by former results concerning the matching experiment of actual image with the comparison of conventional filtering methods.The adaptive search ability of immune genetic algorithm with the elitist selection and elitist crossover(IGAE) was used to optimize threshold t of the transformation function,and then combined with wavelet transformation to estimate noise variance.Multi-experiments were performed to test the validity of IGAE.The results show that the filtered result of t obtained by IGAE is superior to that of t obtained by other methods,IGAE has a faster convergence speed and a higher computational efficiency compared with the canonical genetic algorithm with the elitism and the immune algorithm with the information entropy and elitism by multi-experiments.
基金The National High Technology Research and Development Program of China(‘863’Program)grant number:2007AA02Z4A9+1 种基金National Natural Science Foundation of Chinagrant number:30671997
文摘In recent years,many medical image fusion methods had been exploited to derive useful information from multimodality medical image data,but,not an appropriate fusion algorithm for anatomical and functional medical images.In this paper,the traditional method of wavelet fusion is improved and a new fusion algorithm of anatomical and functional medical images,in which high-frequency and low-frequency coefficients are studied respectively.When choosing high-frequency coefficients,the global gradient of each sub-image is calculated to realize adaptive fusion,so that the fused image can reserve the functional information;while choosing the low coefficients is based on the analysis of the neighborbood region energy,so that the fused image can reserve the anatomical image's edge and texture feature.Experimental results and the quality evaluation parameters show that the improved fusion algorithm can enhance the edge and texture feature and retain the function information and anatomical information effectively.
文摘Through research for image compression based on wavelet analysis in recent years, we put forward an adaptive wavelet decomposition strategy. Whether sub-images are to be decomposed or not are decided by their energy defined by certain criterion. Then we derive the adaptive wavelet decomposition tree (AWDT) and the way of adjustable compression ratio. According to the feature of AWDT, this paper also deals with the strategies which are used to handle different sub-images in the procedure of quantification and coding of the wavelet coefficients. Through experiments, not only the algorithm in the paper can adapt to various images, but also the quality of recovered image is improved though compression ratio is higher and adjustable. When their compression ratios are near, the quality of subjective vision and PSNR of the algorithm are better than those of JPEG algorithm.
文摘A novel optimal image watermarking scheme is proposed in which the genetic algorithm (GA) is employed to obtain the improvement of algorithm performance. Arnold transform is utilized to obtain the scrambled watermark, and then the embedding and extraction of watermark are implemented in digital wavelet transform (DWT) domain. During the watermarking process, GA is employed to search optimal parameters of embedding strength and times of Arnold transform to gain the optimization of watermarking performance. Simulation results show that the proposed method can improve the quality of watermarked image and give almost the same robustness of the watermark.
文摘Aim To fuse the fluorescence image and transmission image of a cell into a single image containing more information than any of the individual image. Methods Image fusion technology was applied to biological cell imaging processing. It could match the images and improve the confidence and spatial resolution of the images. Using two algorithms, double thresholds algorithm and denoising algorithm based on wavelet transform,the fluorescence image and transmission image of a Cell were merged into a composite image. Results and Conclusion The position of fluorescence and the structure of cell can be displyed in the composite image. The signal-to-noise ratio of the exultant image is improved to a large extent. The algorithms are not only useful to investigate the fluorescence and transmission images, but also suitable to observing two or more fluoascent label proes in a single cell.