Malus prunifolia Borkh. ‘Fupingqiuzi’ has significant ecological and economic value and plays a key role in germplasm development and resistance research. However, its long juvenile phase and high heterozygosity are...Malus prunifolia Borkh. ‘Fupingqiuzi’ has significant ecological and economic value and plays a key role in germplasm development and resistance research. However, its long juvenile phase and high heterozygosity are barriers to the identification of ‘Fupingqiuzi’ progeny with excellent traits. In-vitro regeneration techniques and Agrobacterium-mediated genetic transformation systems can efficiently produce complete plants and thus enable studies of gene function.However, optimal regeneration and genetic transformation systems for ‘Fupingqiuzi’ have not yet been developed.Here, we evaluated the factors that affect the in-vitro regeneration and transformation of ‘Fupingqiuzi’. The best results were obtained when transverse leaf sections were used as explants, and they were grown in dark culture for three weeks with their adaxial sides contacting the culture medium(MS basal salts, 30 g Lsucrose, 8 g Lagar, 5 mg L6-benzylaminopurine(6-BA), 2 mg Lthidiazuron(TDZ), and 1 mg L1-naphthlcetic acid(NAA), pH 5.8). A genetic transformation system based on this regeneration system was optimized: after inoculation with A. tumefaciens solution for 8 min, 4 days of co-culture, and 3 days of delayed culture, the cultures were screened with cefotaxime(150 mg L) and kanamycin(15 mg L). We thus established an efficient regeneration and genetic transformation system for ‘Fupingqiuzi’, enabling the rapid production of transgenic material. These findings make a significant contribution to apple biology research.展开更多
目的:研究楸子果实海红果多糖(Polysaccharide from fruit of Malus Prunifolia Borkh,PFM)对衰老模型小鼠脑细胞的保护作用。方法:取昆明小鼠60只,随机分为空白对照组、模型组和高、中、低剂量给药组以及黄芪多糖阳性对照组,以D-半乳...目的:研究楸子果实海红果多糖(Polysaccharide from fruit of Malus Prunifolia Borkh,PFM)对衰老模型小鼠脑细胞的保护作用。方法:取昆明小鼠60只,随机分为空白对照组、模型组和高、中、低剂量给药组以及黄芪多糖阳性对照组,以D-半乳糖制作衰老模型小鼠。观察各组脑组织切片HE染色组织形态学改变,应用流式细胞仪观察脑细胞形态学和细胞凋亡率的变化。结果:PFM各给药组均可见脑细胞神经元树突不同程度恢复正常,神经元体积逐渐增大,核形恢复规则,细胞器丰富,染色质分布较规则,衰老情况得到改善;各给药组细胞凋亡率明显降低,且呈现剂量依赖性。结论:PFM可改善衰老模型小鼠脑组织结构,抑制脑细胞凋亡,降低凋亡率,具有明显的抗脑衰老作用。展开更多
以海红果为原料,探讨了海红果多糖(polysaccharide from Malus prunifolia Borkh,PFM)的超声波提取工艺。以料液比、提取时间、提取温度以及超声功率为考察因素,通过单因素试验和正交试验L9(34)方法,确定了提取海红果多糖的最佳工艺条...以海红果为原料,探讨了海红果多糖(polysaccharide from Malus prunifolia Borkh,PFM)的超声波提取工艺。以料液比、提取时间、提取温度以及超声功率为考察因素,通过单因素试验和正交试验L9(34)方法,确定了提取海红果多糖的最佳工艺条件。结果表明,最佳的提取工艺条件为料液比1:15,超声温度55℃,提取时间50min,超声功率90W。在此条件下,多糖含量平均可达103.9mg/g。展开更多
基金supported by the National Key Research and Development Program of China(2019YFD1001403-4)the Key S&T Special Projects of Shaanxi Province,China(2020zdzx03-01-02)the earmarked fund for the China Agriculture Research System of MOF and MARA(CARS-27)。
文摘Malus prunifolia Borkh. ‘Fupingqiuzi’ has significant ecological and economic value and plays a key role in germplasm development and resistance research. However, its long juvenile phase and high heterozygosity are barriers to the identification of ‘Fupingqiuzi’ progeny with excellent traits. In-vitro regeneration techniques and Agrobacterium-mediated genetic transformation systems can efficiently produce complete plants and thus enable studies of gene function.However, optimal regeneration and genetic transformation systems for ‘Fupingqiuzi’ have not yet been developed.Here, we evaluated the factors that affect the in-vitro regeneration and transformation of ‘Fupingqiuzi’. The best results were obtained when transverse leaf sections were used as explants, and they were grown in dark culture for three weeks with their adaxial sides contacting the culture medium(MS basal salts, 30 g Lsucrose, 8 g Lagar, 5 mg L6-benzylaminopurine(6-BA), 2 mg Lthidiazuron(TDZ), and 1 mg L1-naphthlcetic acid(NAA), pH 5.8). A genetic transformation system based on this regeneration system was optimized: after inoculation with A. tumefaciens solution for 8 min, 4 days of co-culture, and 3 days of delayed culture, the cultures were screened with cefotaxime(150 mg L) and kanamycin(15 mg L). We thus established an efficient regeneration and genetic transformation system for ‘Fupingqiuzi’, enabling the rapid production of transgenic material. These findings make a significant contribution to apple biology research.
文摘目的:研究楸子果实海红果多糖(Polysaccharide from fruit of Malus Prunifolia Borkh,PFM)对衰老模型小鼠脑细胞的保护作用。方法:取昆明小鼠60只,随机分为空白对照组、模型组和高、中、低剂量给药组以及黄芪多糖阳性对照组,以D-半乳糖制作衰老模型小鼠。观察各组脑组织切片HE染色组织形态学改变,应用流式细胞仪观察脑细胞形态学和细胞凋亡率的变化。结果:PFM各给药组均可见脑细胞神经元树突不同程度恢复正常,神经元体积逐渐增大,核形恢复规则,细胞器丰富,染色质分布较规则,衰老情况得到改善;各给药组细胞凋亡率明显降低,且呈现剂量依赖性。结论:PFM可改善衰老模型小鼠脑组织结构,抑制脑细胞凋亡,降低凋亡率,具有明显的抗脑衰老作用。
文摘以海红果为原料,探讨了海红果多糖(polysaccharide from Malus prunifolia Borkh,PFM)的超声波提取工艺。以料液比、提取时间、提取温度以及超声功率为考察因素,通过单因素试验和正交试验L9(34)方法,确定了提取海红果多糖的最佳工艺条件。结果表明,最佳的提取工艺条件为料液比1:15,超声温度55℃,提取时间50min,超声功率90W。在此条件下,多糖含量平均可达103.9mg/g。
