As the‘Green Revolution’gene,SD1(encoding GA20ox2),has been widely applied to improve yield in rice breeding.However,research on its transcriptional regulation is limited.Here,we identified a transcription factor Os...As the‘Green Revolution’gene,SD1(encoding GA20ox2),has been widely applied to improve yield in rice breeding.However,research on its transcriptional regulation is limited.Here,we identified a transcription factor OsbZIP01,which can suppress the expression of SD1 and regulate gibberellin(GA)biosynthesis in rice.Knockout mutants of OsbZIP01 exhibited increased plant height,while the overexpression lines showed a semi-dwarf phenotype and diminished germination rate.Furthermore,the semi-dwarf phenotype of OE-bZIP01,was caused by the reduced internode length,which was accompanied by a thin stem width.The predominant expression of OsbZIP01 was observed in leaves and sheaths.OsbZIP01 protein was localized in the nucleus and showed transcriptional repression activity.In addition,OsbZIP01 could directly bind to the promoter of the OsSD1 gene,and inhibit its transcription.The semi-dwarf phenotype of OE-bZIP01 could be rescued by exogenous GA_(3).Meanwhile,the bzip01 sd1 double mutant showed a shorter shoot length compared with the wild type,indicating that OsbZIP01 regulated plant growth mainly through the GA biosynthesis pathway.Collectively,OsbZIP01 negatively regulates GA biosynthesis by restraining SD1 transcription,thereby affecting plant growth and development.展开更多
以耐铬(VI)菌株Serratia sp. CM01为研究对象,探究其全基因组信息,挖掘其潜在的铬代谢相关基因。本研究采用基因组测序技术对CM01进行全基因组测序并分析其基因序列特征;同时,结合前期差异蛋白研究结果,进行铬代谢相关基因分析。测序结...以耐铬(VI)菌株Serratia sp. CM01为研究对象,探究其全基因组信息,挖掘其潜在的铬代谢相关基因。本研究采用基因组测序技术对CM01进行全基因组测序并分析其基因序列特征;同时,结合前期差异蛋白研究结果,进行铬代谢相关基因分析。测序结果表明,CM01基因组大小为4,902,254 bp,预测编码蛋白序列的基因有4547个;蛋白功能注释结果显示其涉及氧化还原、氨基酸代谢、碳水化合物和能量代谢编码的基因有较高的占比。结合前期蛋白组学的结果,筛选出了12个与铬代谢相关的基因。qRT-PCR分析结果显示,在Cr(VI)胁迫下,ChrA1、Srpc、GrxA和NemA基因的表达上调。CM01基因组全序列已上传至NCBI,序列号:PRJNA675313。本研究通过对CM01的基因组序列分析,为全面了解细菌的铬代谢机制提供基础,为修复环境铬污染的新生物技术提供理论依据。The study focused on the chromium (VI)-resistant bacterial strain Serratia sp. CM01 to explore its whole-genome information and to mine its potential chromium metabolism-related genes. In this research, genomic sequencing technology was employed to perform whole-genome sequencing on CM01 and to analyze its gene sequence characteristics. Additionally, the results from previous differential protein studies were integrated to analyze genes related to chromium metabolism. The sequencing results indicated that the CM01 genome is 4,902,254 base pairs in size, with 4547 genes predicted to encode protein sequences. Protein function annotation revealed a high proportion of genes involved in oxidation-reduction, amino acid metabolism, carbohydrate metabolism, and energy metabolism. Combining the outcomes from previous proteomics studies, 12 genes related to chromium metabolism were identified. Quantitative real-time PCR analysis showed that the expression of ChrA1, Srpc, GrxA, and NemA genes was upregulated under Cr(VI) stress. The complete genome sequence of CM01 has been uploaded to NCBI with the accession number PRJNA675313. Through the genomic sequence analysis of CM01, this study provides a foundation for a comprehensive understanding of bacterial chromium metabolism mechanisms and offers a theoretical basis for the development of new biotechnologies for the remediation of environmental chromium pollution.展开更多
基金supported by the National Natural Science Foundation of China (No. 52171119)the Natural Science Foundation of Jiangsu Province, China (No. BK20201308)。
基金supported by the National Natural Science Foundation of China(Grant No.32101763)China National Postdoctoral Program for Innovative Talents(Grant No.BX2021266)China Postdoctoral Science Foundation(Grant No.2021M692853).
文摘As the‘Green Revolution’gene,SD1(encoding GA20ox2),has been widely applied to improve yield in rice breeding.However,research on its transcriptional regulation is limited.Here,we identified a transcription factor OsbZIP01,which can suppress the expression of SD1 and regulate gibberellin(GA)biosynthesis in rice.Knockout mutants of OsbZIP01 exhibited increased plant height,while the overexpression lines showed a semi-dwarf phenotype and diminished germination rate.Furthermore,the semi-dwarf phenotype of OE-bZIP01,was caused by the reduced internode length,which was accompanied by a thin stem width.The predominant expression of OsbZIP01 was observed in leaves and sheaths.OsbZIP01 protein was localized in the nucleus and showed transcriptional repression activity.In addition,OsbZIP01 could directly bind to the promoter of the OsSD1 gene,and inhibit its transcription.The semi-dwarf phenotype of OE-bZIP01 could be rescued by exogenous GA_(3).Meanwhile,the bzip01 sd1 double mutant showed a shorter shoot length compared with the wild type,indicating that OsbZIP01 regulated plant growth mainly through the GA biosynthesis pathway.Collectively,OsbZIP01 negatively regulates GA biosynthesis by restraining SD1 transcription,thereby affecting plant growth and development.
文摘以耐铬(VI)菌株Serratia sp. CM01为研究对象,探究其全基因组信息,挖掘其潜在的铬代谢相关基因。本研究采用基因组测序技术对CM01进行全基因组测序并分析其基因序列特征;同时,结合前期差异蛋白研究结果,进行铬代谢相关基因分析。测序结果表明,CM01基因组大小为4,902,254 bp,预测编码蛋白序列的基因有4547个;蛋白功能注释结果显示其涉及氧化还原、氨基酸代谢、碳水化合物和能量代谢编码的基因有较高的占比。结合前期蛋白组学的结果,筛选出了12个与铬代谢相关的基因。qRT-PCR分析结果显示,在Cr(VI)胁迫下,ChrA1、Srpc、GrxA和NemA基因的表达上调。CM01基因组全序列已上传至NCBI,序列号:PRJNA675313。本研究通过对CM01的基因组序列分析,为全面了解细菌的铬代谢机制提供基础,为修复环境铬污染的新生物技术提供理论依据。The study focused on the chromium (VI)-resistant bacterial strain Serratia sp. CM01 to explore its whole-genome information and to mine its potential chromium metabolism-related genes. In this research, genomic sequencing technology was employed to perform whole-genome sequencing on CM01 and to analyze its gene sequence characteristics. Additionally, the results from previous differential protein studies were integrated to analyze genes related to chromium metabolism. The sequencing results indicated that the CM01 genome is 4,902,254 base pairs in size, with 4547 genes predicted to encode protein sequences. Protein function annotation revealed a high proportion of genes involved in oxidation-reduction, amino acid metabolism, carbohydrate metabolism, and energy metabolism. Combining the outcomes from previous proteomics studies, 12 genes related to chromium metabolism were identified. Quantitative real-time PCR analysis showed that the expression of ChrA1, Srpc, GrxA, and NemA genes was upregulated under Cr(VI) stress. The complete genome sequence of CM01 has been uploaded to NCBI with the accession number PRJNA675313. Through the genomic sequence analysis of CM01, this study provides a foundation for a comprehensive understanding of bacterial chromium metabolism mechanisms and offers a theoretical basis for the development of new biotechnologies for the remediation of environmental chromium pollution.
