Water Extraction Process of Chinese Herbal Compound Man Gan Ning

[Objectives]To optimize the water extraction process of Chinese Herbal Compound Man Gan Ning and establish a method for its extraction and content determination.[Methods]The L9(34)orthogonal test was carried out,the comprehensive scores of ginsenoside Rb1 content,notoginsenoside R1 content,tanshinone I content and saikosaponin A content in the compound extract were used as evaluation indicators to explore the effects of water addition,the water addition,decoction time and decoction times on the water extraction process,and verification tests were carried out.[Results]The optimized water extraction process was as follows:adding 9 times of water in the first extraction,and extracting for 90 min;adding 7 times of water in the second extraction,and extracting for 60 min;adding 7 times of water in the third extraction,and extracting for 60 min.[Conclusions]The optimized water extraction process is stable and feasible,and can be used for the extraction of various herbs in Compound Man Gan Ning.It can also provide experimental basis for the formulation development of Compound Man Gan Ning sustained release tablet.

慢肝宁抗肝纤维化的肝组织形态定量及α-平滑肌肌动蛋白免疫组化评价

目的 :探讨慢肝宁胶囊抗肝纤维化的作用机制。方法 :让 3 0例慢性肝炎患者服用慢肝宁胶囊治疗 6个月 ,设对照组 10例 ,用USF图像分析仪对治疗前后两次肝活检标本以点计数法作组织形态定量及α SMA (α 平滑肌肌动蛋白 )阳性细胞数密度测定。结果 :治疗组良好肝细胞面密度增高非常显著 (P <0 .0 0 1) ,变性肝细胞、纤维组织、淋巴细胞面密度减少亦非常显著 (P <0. 0 1) ,肝组织α SMA阳性细胞数密度也显著减少 (P <0. 0 2 ) ;而对照组除变性肝细胞面密度有显著减少外其他变化不显著。结论 :慢肝宁可能是通过抑制淋巴细胞等炎细胞及活化肝星状细胞活性来发挥抗肝细胞损伤及抗纤维化作用。