This study evaluates the hepatic effects of Mandragora officinarum leaf extract on wistar albino rats. A total of twenty-four (24) rats were randomly divided into 4 groups labeled A, B, C and D and kept in a well-vent...This study evaluates the hepatic effects of Mandragora officinarum leaf extract on wistar albino rats. A total of twenty-four (24) rats were randomly divided into 4 groups labeled A, B, C and D and kept in a well-ventilated room. Group A served as control and these rats were fed distilled water. Rats in groups B, C, and D were given three (3) different doses of the leaf extract (1.5, 3.5 and 5.0 mL/KgBW) respectively. They were administered once daily for 14 and 28 days consecutively. Animals were sacrificed 24 hours after the last treatment. Blood samples were collected into heparinized sample bottles for analysis. Aspartate aminotransferase, Alanine aminotransferase and histology results were normal when the leaf extract was given for 14 days. Alkaline phosphatase significantly decreased within the same time interval. Alkaline phosphatase increased in a dose-dependent manner for 28 days. All other liver enzymes were within normal limits. Histopathological changes were seen in all doses when Mandragora officinarum leaf extract was used for 28 days. These changes also worsened with increasing doses. This suggests that the use of mandragora officinarum leaf extract for long periods at a time can cause hepatic damage.展开更多
【目的】挖掘高海拔、低温度下生长的青海茄参(Mandragora chinghaiensis Kuang et A.M.Lu)中的光合相关抗寒基因。【方法】以0℃低温胁迫青海茄参为试验材料,通过光合生理试验寻找关键时间点,进而进行转录组学分析,筛选光合相关抗寒基...【目的】挖掘高海拔、低温度下生长的青海茄参(Mandragora chinghaiensis Kuang et A.M.Lu)中的光合相关抗寒基因。【方法】以0℃低温胁迫青海茄参为试验材料,通过光合生理试验寻找关键时间点,进而进行转录组学分析,筛选光合相关抗寒基因并进行实时荧光定量(qRT⁃PCR)分析。【结果】净光合速率、气孔导度、蒸腾速率、最大荧光、光系统II最大光化学效率、光化学猝灭系数以及电子传递速率呈逐渐下降趋势,胞间CO_(2)浓度、初始荧光和非光化学猝灭系数呈上升趋势,且根据光合参数及叶绿素荧光参数的变化综合分析出关键时间节点为12 h,根据转录组分析筛选出7个基因,通过qRT⁃PCR分析表明,CP43基因表达先上调后下调,整体呈上调趋势;CAB⁃8、CAB⁃11、CP24⁃10B、CH⁃02、FD⁃C2与PSI⁃D2基因表达先下调后上调,整体呈下调趋势。【结论】本研究结果为青海茄参筛选光合相关抗寒基因以及后续的转基因验证提供了理论支持,达到了筛选基因的主要目的。展开更多
文摘This study evaluates the hepatic effects of Mandragora officinarum leaf extract on wistar albino rats. A total of twenty-four (24) rats were randomly divided into 4 groups labeled A, B, C and D and kept in a well-ventilated room. Group A served as control and these rats were fed distilled water. Rats in groups B, C, and D were given three (3) different doses of the leaf extract (1.5, 3.5 and 5.0 mL/KgBW) respectively. They were administered once daily for 14 and 28 days consecutively. Animals were sacrificed 24 hours after the last treatment. Blood samples were collected into heparinized sample bottles for analysis. Aspartate aminotransferase, Alanine aminotransferase and histology results were normal when the leaf extract was given for 14 days. Alkaline phosphatase significantly decreased within the same time interval. Alkaline phosphatase increased in a dose-dependent manner for 28 days. All other liver enzymes were within normal limits. Histopathological changes were seen in all doses when Mandragora officinarum leaf extract was used for 28 days. These changes also worsened with increasing doses. This suggests that the use of mandragora officinarum leaf extract for long periods at a time can cause hepatic damage.
