Comparative Study on Tissue Distribution of Tibetan Medicines Renqing Mangjue Compatible with Zuota

[Objectives]To explore the mechanism for the attenuate-synergistic effect of Zuota to Renqing Mangjue,a contrasted study on the tissue distribution of Tibetan medicines Renqing Mangjue compatible with Zuota was carried out. [Methods] The SD rats gastric ulcer model were made successfully,and then respectively were given Renqing Mangjue compatible with Zuota or not according to the dose of 0. 144 g/kg and normal saline by gavage administration,once a day. After 14 d of administration,various organs and tissue were isolated,including brain,heart,liver,spleen,lung,kidney,jejunum,skeletal muscle,fat,skin,submandibular gland,uterus and ovary( or testicle and epididymis).The LC-MS/MS methods were used for determining the contents of brucine and strychnine in various organs and tissue,and significant difference analysis,main target site analysis and clustering analysis were implemented. [Results]Between the Zuota group and non-Zuota group,there was extremely significant difference in the content of brucine in the liver,kidney,muscle,lung,ovary,and heart( P < 0. 01),significant difference in the jejunum( P < 0. 05),and no significant difference in the epididymis,testis,brain,skin and uterus( P > 0. 05); the content of brucine in the submandibular gland,spleen,liver and ovary increased significantly,but significantly reduced in the kidney,muscle,jejunum,lung and heart. And there was extremely significant difference in the content of strychnine in the liver,kidney,ovary,brain and heart( P < 0. 01),and no significant difference in the uterus( P > 0. 05); the strychnine content increased significantly in the liver,spleen and ovary,and significantly decreased in the kidney,muscle,testis,lung,brain and heart.[Conclusions] Compatibility of Zuota can affect the distribution of brucine and strychnine in some tissue and organs,so as to achieve attenuate-synergistic effect of Zuota to Renqing Mangjue.