Mannose-binding lectin and maladies of the bowel and liver

Mannose-binding lectin (MBL) is a pattern-recognition molecule that binds to characteristic carbohydrate mo-tifs present on the surface of many different pathogens. MBL binding stimulates the immune system via the lectin pathway of complement activation. In certain clinical situations, often characterized by pre-existing immune compromise, MBL deficiency increases the risk of infec-tious and other disease-specific complications. Many of the key pathogenic processes inherent to common gastroenterological diseases, such as infection, immuno-logical damage, and carcinogenesis, have been linked to MBL. This editorial reviews the biology of MBL, outlines key disease associations to document the breadth of influence of MBL, and finally, highlights the relevance of MBL to both gastroenterological health and disease.

Mannose-binding Lectin Two Gene Polymorphisms and Tuberculosis Susceptibility in Chinese Population: A Meta-analysis

Numerous studies have been done to explore the association between mannose-binding lectin two （MBL2） gene polymorphisms and the risk of tuberculosis （TB）. However, the results are inconsistent. We performed a meta-aualysis to investigate whether polymorphisms in the MBL2 gene were associated with TB risk. Databases including PubMed, Medline, Chinese Biomedicine Database, China National Knowledge Infrastructure, Wanfang Database, and Weipu Database were searched to find relevant articles published up to 2 October, 2012. Odds ratio （OR） with 95% confidence interval （CI） was used to evaluate the strength of association. All statistical tests were performed by using Revman 5.1 software and STATA 11.0 software. Six case-control studies including 1106 cases and 1190 controls were accepted in the meta-analysis. The results indicated that individuals carrying the MBL2 codon 54 B allele may have an increased risk of TB as compared with AA homozygotes （BB＋AB vs. AA： OR=1.52, 95% CI： 1.22-1.88）, whereas MBL2 ＋4 P/Q was possibly not associated with TB susceptibility in Chinese population.

Mannose-binding lectin 2 rs11003123 polymorphism is associated with the development of hepatocellular carcinoma in patients with hepatitis B-related cirrhosis in the Chinese population

BACKGROUND： Mannose-binding lectin 2 （MBL2） plays a key role in the host immune response, but whether it is associ- ated with hepatocellular carcinoma （HCC） is not dear. The present study aimed to identify the association between MBL2 gene polymorphisms and HCC in patients with hepatitis B virus （I-IBV）-related cirrhosis in the Chinese population.

Early expression of mannose-binding lectin 2 during Aspergillus fumigatus infection in human corneal epithelial cells

AIM: To evaluate the early expression of mannose-binding lectin 2(MBL2) in human corneal epithelial cells(HCECs) infected by Aspergillus fumigatus(AF).METHODS: HCECs cultured in vitro with AF antigens and sampled at 0, 0.5, 1, 2, 4, 6 and 8h. The expression of MBL2 m RNA was evaluated by semiquantitative reverse transcription-polymerase chain reaction(RT-PCR). The expression of MBL2 protein in supernatant fluid was shown by enzyme linked immunosorbent assay(ELISA). MBL2 protein in HCECs was detected by immunocytochemistry at 0 and 24 h.RESULTS: MBL2 m RNA and protein are expressed in normal HCECs. The expression of MBL2 m RNA and protein in supernatant fluid begin to increase after being stimulated with AF antigens. The most significantly peak of MBL2 m RNA is in 2h. The protein of MBL2 in supernatant fluid decrease gradually after 0.5h. The protein in HCECs expression increase after stimulation of24 h.· CONCLUSION: MBL2 receptor expressed in normal HCECs in vitro. The stimulation by AF antigens can increase the early expression of it.

Expressions and clinical significances of mannose-binding lectin(MBL) and MBL-associated serine protease 2(MASP-2) in patients with thyroid neoplasm

Objective： The aim of the study was to detect the levels of mannose-binding lectin （MBL）, MBL-associated serine protease 2 （MASP-2） and explore the clinical significances of them in patients with primary thyroid neoplasms. Methods： By using ELISA method, we detected the serum levels of MBL and MASP-2 in 26 patients with papillary thyroid carcinoma （PTC）, 30 patients with thyroid adenoma （TA） and 26 healthy people, respectively. Results： Serum MBL level was （565.23 ± 76.70） μg/L in PTCs higher than （324.267 ±24.74） μg/L in TAs, and （152.69± 16.95） IJg/L in healthy of controlling group. There was statistical significance between PTC and TA （P 〈 0.05）, however there was no difference between TA and healthy （P 〉 0.05）. Serum MASP-2 level was （726.153± 78.88） pg/L in PTCs higher than （379.266 ± 30.26） μg/L in TAs, and （203.846 ± 29.09） μg/L in healthy. Serum MASP-2 level was higher in PTCs than TAs, and the difference had statistical significance （P 〈 0.01）. But no difference was observed between in TAs and healthy. Conclusion： These findings might reflect inflammatory processes induced by defense mechanisms, in response to the development of the turnout. MBL may also be involved in the elimination of possible tumourigenic pathogens.

Dietary sugars inhibit biologic functions of the pattern recognition molecule, mannose-binding lectin

Mannose-binding lectin (MBL), a mammalian lectin, is a pattern recognition molecule of the innate immune system and recognizes carbo-hydrates that are exposed on pathogens. In this study, we observed that fructose down regu-lates MBL-mediated innate immune mechanisms against both influenza A virus (IAV) and Staphy-lococcus aureus. These mechanisms include the lectin complement pathway and coagulation enzyme-like activities on both pathogens. Fur-thermore, fructose also reduces MBL-mediated phagocytosis of S. aureus and IAV and MBL- mediated IAV infection to epithelial cells. In contrast, sucrose inhibits MBL-mediated im-mune mechanisms against S. aureus but not IAV. Together, our studies show that dietary sugars, in particular fructose, negatively regulate the innate immunity against viral and bacterial pathogens.

Mannose-binding lectin 2 gene polymorphisms and their association with tuberculosis in a Chinese population

Background:Immune-and inflammation-related genes(IIRGs)play an important role in the pathogenesis of tuberculosis(TB).However,the relationship between IIRG polymorphisms and TB risk remains unknown.In this study,the gene polymorphisms and their association with tuberculosis were determined in a Chinese population.Methods:We performed a case-control study involving 1016 patients with TB and 507 healthy controls of Han Chinese origin.Sixty-four single-nucleotide polymorphisms(SNPs)belonging to 18 IIRGs were genotyped by the PCR-MassArray assay,and the obtained data was analyzed withχ2-test,Bonferroni correction,and unconditional logistic regression analysis.Results:We observed significant differences in the allele frequency of LTA rs2229094*C(P=0.015),MBL2 rs2099902*C(P=0.001),MBL2 rs930507*G(P=0.004),MBL2 rs10824793*G(P=0.004),and IL12RB1 rs2305740*G(P=0.040)between the TB and healthy groups.Increased TB risk was identified in the rs930507 G/G genotype(Padjusted=0.027)under a codominant genetic model as well as in the rs2099902(C/T+C/C)vs T/T genotype(Padjusted=0.020),rs930507(C/G+G/G)vs C/C genotype(Padjusted=0.027),and rs10824793(G/A+G/G)vs A/A genotype(Padjusted=0.017)under a dominant genetic model after Bonferroni correction in the analysis of the overall TB group rather than the TB subgroups.Furthermore,the rs10824793_rs7916582*GT and rs10824793_rs7916582*GC haplotypes were significantly associated with increased TB risk(P=0.001,odds ratio[OR]=1.421,95%confidence interval[CI]:1.152-1.753;and P=0.018,OR=1.364,95%CI:1.055-1.765,respectively).Moreover,the rs10824793_rs7916582*AT/AT or rs10824793_rs7916582*GT/GT diplotype showed a protective(P=0.003,OR=0.530,95%CI:0.349-0.805)or harmful(P=0.009,OR=1.396,95%CI:1.087-1.793)effect against the development of TB.Conclusions:This study indicated that MBL2 polymorphisms,haplotypes,and diplotypes were associated with TB susceptibility in the Han Chinese population.Additionally,larger sample size studies are needed to further confirm these findings in the future.

Establishment of a Multiplex Detection Method for Common Bacteria in Blood Based on Human Mannan-Binding Lectin Protein-Conjugated Magnetic Bead Enrichment Combined with Recombinase-Aided PCR Technology

Objective Recombinase-aided polymerase chain reaction(RAP)is a sensitive,single-tube,two-stage nucleic acid amplification method.This study aimed to develop an assay that can be used for the early diagnosis of three types of bacteremia caused by Staphylococcus aureus(SA),Pseudomonas aeruginosa(PA),and Acinetobacter baumannii(AB)in the bloodstream based on recombinant human mannanbinding lectin protein(M1 protein)-conjugated magnetic bead(M1 bead)enrichment of pathogens combined with RAP.Methods Recombinant plasmids were used to evaluate the assay sensitivity.Common blood influenza bacteria were used for the specific detection.Simulated and clinical plasma samples were enriched with M1 beads and then subjected to multiple recombinase-aided PCR(M-RAP)and quantitative PCR(qPCR)assays.Kappa analysis was used to evaluate the consistency between the two assays.Results The M-RAP method had sensitivity rates of 1,10,and 1 copies/μL for the detection of SA,PA,and AB plasmids,respectively,without cross-reaction to other bacterial species.The M-RAP assay obtained results for<10 CFU/mL pathogens in the blood within 4 h,with higher sensitivity than qPCR.M-RAP and qPCR for SA,PA,and AB yielded Kappa values of 0.839,0.815,and 0.856,respectively(P<0.05).Conclusion An M-RAP assay for SA,PA,and AB in blood samples utilizing M1 bead enrichment has been developed and can be potentially used for the early detection of bacteremia.

胸腔积液沉渣包埋组织TTF-1、Galectin-3的表达水平与肺腺癌恶性生物学行为间的关系

目的探讨胸腔积液沉渣包埋组织甲状腺转录因子1(TTF-1)、半乳糖凝集素-3(Galectin-3)的表达水平与肺腺癌恶性生物学行为间的关系。方法选取2020年10月至2023年3月在该院就诊的肺腺癌患者163例,根据分化程度分为高分化组(26例)、中分化组(78例)、低分化组(59例)。对比3组胸腔积液沉渣包埋组织TTF-1、Galectin-3及恶性生物学行为相关基因(p16基因mRNA)表达水平,Spearman秩相关系数分析胸腔积液沉渣包埋组织TTF-1、Galectin-3与p16基因mRNA表达水平的相关性,多元线性回归分析胸腔积液沉渣包埋组织TTF-1、Galectin-3与p16基因mRNA表达水平间的依存关系,限制性立方样条图分析胸腔积液沉渣包埋组织TTF-1、Galectin-3与p16基因mRNA表达水平间的剂量-效应关系。结果低分化组胸腔积液沉渣包埋组织TTF-1、Galectin-3表达水平高于中分化组、高分化组,p16基因mRNA表达水平低于中分化组、高分化组,中分化组胸腔积液沉渣包埋组织TTF-1、Galectin-3表达水平高于高分化组,p16基因mRNA表达水平低于高分化组,差异有统计学意义(P<0.05);胸腔积液沉渣包埋组织TTF-1(r=-0.752,P<0.001)、Galectin-3(r=-0.811,P<0.001)表达水平与p16基因mRNA表达水平呈负相关;胸腔积液沉渣包埋组织Galectin-3表达水平与p16基因mRNA表达水平间无线性依存关系(P>0.05),胸腔积液沉渣包埋组织TTF-1表达水平与p16基因mRNA表达水平间有线性依存关系(P=0.049);当胸腔积液沉渣包埋组织TTF-1表达水平<4分、胸腔积液沉渣包埋组织Galectin-3表达水平<3分时,对p16基因mRNA表达作用最显著。结论胸腔积液沉渣包埋组织TTF-1、Galectin-3表达水平与肺腺癌恶性生物学行为关系密切,早期检测对临床实现精准干预避免病情恶化具有重要意义。

The lectin gene TRpL1 of tetraploid Robinia pseudoacacia L.response to salt stress

Lectins are natural proteins in animals,plants,and microorganisms and can be divided into 12 families.These lectins play important roles in various environmental stresses.Some polyploid plants show tolerance to environmental stresses and to insect pests.However,the mechanism of stress tolerance is unclear.Tetraploid Robinia pseudoacacia(4×)under salt stress showed higher tolerance than diploid R.pseudoacacia(2×).As lectin can improve stress tolerance,it was questioned whether the stress resistance of polyploid plants was related to the lectin protein.In this study,salt resistance of lectin gene TRpL1 was verified by its over-expression in plants.In addition,salt resistance of lectin protein by E.coli strains was detected.The data revealed that the over-expression transgenic plants of TRpL1 showed better salt tolerance than control plants under salt stress,and the TRpL1-expressing strain also grew better in the medium with added NaCl.Therefore,tetraploid plants can resist salt stress through TRpL1 protein regulation.

Characterization of a CD209-Like Lectin in Black Rockfish(Sebastes schlegelii):Structure Features and Binding Activities as a Pathogen Recognition Receptor

CD209,a transmembrane lectin belonging to the C-type lectin family,can recognize carbohydrates on the surface of host cells and invading pathogens,and play an important role in cell adhesion and migration,pathogen recognition and immune activation.Although well characterized in mammals,CD209 is still under-researched in fish.Here,we report a CD209-like gene,which was named SsCD209like,in black rockfish Sebastes schlegelii,and analyzed its structure features,expression patterns and ligand-binding activities.SsCD209like displays structural similarities to mammalian CD209s,with a cytosolic tail at N-terminus,a transmembrane region and an extracellular part containing a neck region and a CRD at C-terminus.The extracellular region and the neck region of SsCD-209like can both form dimers,which is different with the tetramer in human homologue.This result demonstrates the multimerization of CD209 homologue in fish for the first time.The EPN motif,a functional motif participating in sugar binding and affinity determination,is conserved in the CRD of SsCD209like,which is consistent with the higher binding strength of this lectin to L-fucose,D-GlcNAc and D-mannose.The binding of SsCD209like to different bacteria strains and bacteria-derived pathogen associated molecular patterns(PAMPs)are also observed in a dose-dependent manner.Results in this study show the sequence and structure features of SsCD209like and demonstrate its binding properties as a pathogen recognition receptor,which promotes our understanding of CD209 homologues in fish and provides basis for more in-depth studies of this molecule in the future.

A novel lectin from mushroom Phellodon melaleucus displays hemagglutination activity,and antitumor activity in a B16 melanoma mouse model

A novel lectin(termed PML)was purified from fruiting bodies of the edible mushroom Phellodon melaleucus(division Basidiomycota)by ion exchange,hydrophobic interaction,and gel filtration chromatographies,with overall titer recovery~60%and 20-fold purification.PML displayed hemagglutination activity 13319 units/mg toward rabbit erythrocytes.SDS-PAGE and gel filtration analyses revealed that PML is a homodimeric lectin with a molecular weight of 28.8 kDa.PML hemagglutination activity was not inhibited by various simple sugars or their derivatives,but was enhanced by cations Ca^(2+),Mg^(2+),Zn^(2+),and Cu^(2+).The activity was stable in pH range 6–9 and in the temperature range 20–60°C.Circular dichroism(CD)spectroscopic analysis showed that PML was composed primarily ofβ-sheets with lowα-helix content.In a B16 melanoma mouse model,PML treatment significantly inhibited tumor growth,and increased cytokine IL-10 content.Our findings suggest that PML is a potential anticancer therapeutic agent.

磁共振成像灌注参数与COL5A2、Galectin-3的相关性及对食管癌的诊断价值

目的探讨磁共振成像(MRI)灌注参数与V型胶原蛋白α2链(COL5A2)、半乳糖苷凝集素-3(Galectin-3)的相关性及对食管癌的诊断价值。方法选取2020年12月—2021年12月我院收治的疑似食管癌患者100例,以内镜和活检病理检查为金标准,最终确诊为食管癌患者的55例为观察组,其余45例食管炎病变患者为对照组。所有患者行磁共振成像检查及COL5A2、Galectin-3水平测定,对比两组MRI灌注参数,分析食管癌患者MRI灌注参数与肿瘤标志物水平的相关性,分析MRI灌注参数对食管癌的诊断价值。结果与对照组相比,观察组K^(trans)、K_(ep)、V_(e)水平较高(P<0.05);与Ⅰ~Ⅱ期食管癌患者相比,Ⅲ~Ⅳ期食管癌患者K trans、K ep、V e水平较高(P<0.05);与Ⅰ~Ⅱ期食管癌患者相比,Ⅲ~Ⅳ期食管癌COL5A2、Galectin-3水平升高(P<0.05);食管癌患者K^(trans)、K_(ep)、V_(e)、COL5A2、Galectin-3均与病情分期呈正相关(P<0.05);K^(trans)、K_(ep)、V_(e)均与COL5A2、Galectin-3水平呈正相关,差异具有统计学意义(P<0.05);与磁共振成像、COL5A2、Galectin-3单一诊断相比,联合诊断对食管癌的预测价值较高(P<0.05)。结论MRI灌注参数与血清Galectin-3、COL5A2三者联合检测准确度及灵敏度较高于单一检测,弥补了单一检测的不足,三者联合对不同分期食管癌患者具有一定的诊断价值。

Effect of a Thermal Spring Water on Carbohydrate-Protein Interactions in In-Vitro Models Implicating Normal Human Keratinocytes and Recombinant Lectins

Background: Sugar moiety of macromolecules is today very well known for its implications in many biological recognition mechanisms including cell-cell, extracellular matrix-cell and/or bacteria-cell interactions. In this context lectins, which are carbohydrate-binding proteins displaying a high affinity for sugar groups of other molecules, are of a great importance, notably in immune response involving bacteria, viruses and fungi. As protein-carbohydrate interactions are often mediated by ions such as calcium, zinc or magnesium, we were prompted to study the effect of a thermal spring water (which contains this type of component) on interactions existing between: 1) osidic receptors of human normal keratinocytes and 2) two lectins greatly implicated in the immune response mechanisms (i.e. the dectin-1 and the langerin), and their ligands. Materials and Methods: In a first series of experiments, we studied the effect of increasing concentrations of a thermal spring water on interactions existing between glycosylated molecules and the osidic receptors expressed at the normal human keratinocytes surface. In a second step, and in order to better understand the putative effect of our thermal spring water on the immune response, we analyzed its effect on the interactions existing between the dectin-1 (implicated in the recognition of bacteria, viruses and fungi) and the langerin (expressed by Langerhans cells, the immune cells of the cutaneous tissue), and their ligands in a model using recombinant human lectins and appropriate binding molecules. Results: We showed here that our thermal spring water was able to reinforce interactions between keratinocytes osidic receptors and some of their ligands, in a dose-related manner: From 8% to 55% of increase with 10% to 30% (v/v) of thermal spring water. In the second part of our studies, we also showed that our thermal spring water was able to modulate interactions between dectin-1 and langerin and their ligands through a biphasic effect: Interactions were enhanced by more than 40% and 20% respectively with 10% of thermal spring water, and return to their basal level or lower for higher concentrations. Conclusion: The tested thermal spring water, probably due to its ionic composition, could significantly affect interactions of osidic receptors with their ligands. This property could be of a great interest to help immune system to maintain an appropriate “vigilance state” by using the thermal water at up to a concentration of 10%, and by avoiding any runaway reaction in case of aggression, by using concentrations higher than 10%. .

血清干扰素γ诱导蛋白10和甘露糖结合凝集素在再生障碍性贫血和骨髓增生异常综合征患者中的含量及临床意义

目的探讨血清干扰素γ诱导蛋白10(IP10)和甘露糖结合凝集素(MBL)在再生障碍性贫血(AA)和骨髓增生异常综合征(MDS)患者中的含量及临床意义。方法选取2019年1月至2021年6月在昆山市第三人民医院初诊的26例AA患者(AA组)和42例MDS患者(MDS组)作为观察组,另选取30名健康体检者作为对照组。检测并比较两组血清IP10和MBL水平,分析两组血清IP10与MBL的相关性,比较两组不同淋巴细胞亚群比例及CD4^(+)、CD8^(+)淋巴细胞比值。结果AA组和MDS组血清IP-10水平均高于对照组,血清MBL水平均低于对照组,且AA组血清IP-10水平高于MDS组,AA组血清MBL水平低于MDS组,差异有统计学意义(P<0.05)。Pearson线性相关分析结果显示,两组血清IP-10与MBL均呈负相关性(r<0,P<0.05)。AA组外周血总CD3^(+)T淋巴细胞比例高于MDS组,差异有统计学意义(P<0.05);两组外周血CD3-CD16+/CD56+NK淋巴细胞比例和CD19+B淋巴细胞比例比较差异无统计学意义。AA组CD3^(+)CD8^(+)T淋巴细胞比例高于MDS组,而CD4^(+)/CD8^(+)比值低于MDS组,差异有统计学意义(P<0.05)。结论通过检测患者血清IP-10和MBL水平,以及分析外周血T淋巴细胞亚群CD4^(+)/CD8^(+)比值,有助于为AA及MDS的发病机制、早期鉴别诊断等方面提供更有价值的信息。

基于ox-LDL/LOX-1信号通路探讨脂质代谢紊乱促进肺癌进展中的机制

目的基于氧化低密度脂蛋白(ox-LDL)/人凝集素样氧化低密度脂蛋白受体1(LOX-1)信号通路探讨脂质代谢紊乱促进肺癌进展的机制。方法收集81个已鉴定的具有成对相邻非癌组织(离肿瘤至少5 cm)的肺腺癌组织,使用免疫组织化学检测LOX-1表达。肺腺癌细胞系(A549、H1299细胞)中过表达LOX-1。用Transwell测定细胞侵袭能力。用不同浓度oxLDL处理细胞,并检测细胞中LOX-1表达情况。结果在包含原发性人肺癌和匹配的邻近非癌组织中,肿瘤中LOX-1染色比非癌组织样品明显增强(中值H分数99.4 vs.16.2,P<0.001)。高LOX-1表达与低生存显著相关(P<0.001)。与无淋巴结转移的患者相比,发生淋巴结转移患者的癌组织具有更高的LOX-1水平(中值H分数83.2 vs.121.1,P<0.01)。LOX-1过表达显著促进肺癌细胞的侵袭转移细胞数(P<0.01)。此外,LOX-1是ox-LDL诱导的肺癌细胞转移所必需的功能靶点。伊他替尼抑制LOX-1过表达的A549在体外的转移能力。结论LOX-1的表达随着oxLDL水平的升高而增加,并且LOX-1的表达上调促进了肺癌细胞的转移,其作用机制可能与激活Janus激酶/转录因子激活子(JAK1/STAT6)信号通路有关。

血清MBL、HRG、IL-23/IL-17炎症轴与动脉瘤性蛛网膜下腔出血患者介入栓塞术后脑血管痉挛和预后的关系

目的探讨血清甘露聚糖结合凝集素(MBL)、富组氨酸糖蛋白(HRG)、白细胞介素(IL)-23/IL-17炎症轴与动脉瘤性蛛网膜下腔出血(aSAH)患者介入栓塞术后脑血管痉挛(CVS)和预后的关系。方法选取2019年3月至2022年2月该院收治的195例行介入栓塞术治疗的aSAH患者,根据术后第4天数字减影血管造影检查是否发生CVS及严重程度分为无CVS组(126例)、轻度CVS组(18例)、中度CVS组(39例)和重度CVS组(12例)。比较4组术前及术后3 d血清MBL、HRG、IL-23、IL-17水平。随访6个月,根据患者预后的不同分为预后良好组(137例)和预后不良组(58例)。采用多因素Logistic回归模型分析aSAH患者预后不良的影响因素。采用受试者工作特征(ROC)曲线分析血清MBL、HRG、IL-23、IL-17水平及其联合应用模型对aSAH患者预后不良的预测价值。结果195例aSAH患者介入栓塞术后CVS发生率为35.38%。术后3 d,轻、中、重度CVS组血清MBL、IL-23、IL-17水平高于无CVS组,其中重度CVS组高于中度CVS组,中度CVS组高于轻度CVS组(P<0.05);轻、中、重度CVS组血清HRG水平低于无CVS组,其中重度CVS组低于中度CVS组,中度CVS组低于轻度CVS组(P<0.05)。术后3 d,4组血清MBL、IL-23、IL-17水平高于术前,血清HRG水平低于术前(P<0.05)。预后良好组动脉瘤直径≥6 mm、动脉瘤个数>1个、手术时间>24 h、Hunt-Hess分级Ⅲ/Ⅳ级、术后CVS患者例数占比及术后3 d血清MBL、IL-23、IL-17水平均低于预后不良组,术后3 d血清HRG水平高于预后不良组(P<0.05)。多因素Logistic回归分析显示,动脉瘤直径≥6 mm、Hunt-Hess分级Ⅲ/Ⅳ级、术后CVS、术后3 d MBL、IL-23、IL-17水平升高及HRG水平降低均是导致aSAH患者预后不良的独立危险因素(P<0.05)。ROC曲线结果显示,术后3 d血清MBL、HRG、IL-23、IL-17这4项指标对aSAH患者预后不良均有一定的预测效能。4项指标联合应用的预测模型效能较高(曲线下面积为0.853)。结论aSAH患者介入栓塞术后血清MBL、IL-23、IL-17水平升高及HRG水平下降可导致CVS发生风险增加,且与aSAH患者介入栓塞术后的预后不良有关。上述指标对aSAH患者预后不良有一定的预测效能。

血清Gal-3、CysC水平预测急性心肌梗死PCI术后急性肾损伤的价值

目的探讨血清半乳糖集素-3(Gal-3)、胱抑素C(CysC)水平预测急性心肌梗死(AMI)冠状动脉介入术(PCI)后急性肾损伤(AKI)的价值。方法回顾性分析2018年5月至2023年2月郑州大学第二附属医院内科重症监护室行急诊PCI术的90例AMI患者临床资料,经过多因素logistic回归分析,明确AMI患者PCI术后发生AKI的危险因素。结果依据AKI的诊断标准进行分组,分为AKI组(20例)和非AKI组(70例)。AKI组患者高血压、糖尿病、术前使用血管紧张素转化酶抑制剂(ACEI)、血肌酐>100μmol·L^(-1)占比高于非AKI组,差异有统计学意义(P<0.05);AKI组患者血清Gal-3、CysC水平高于非AKI组,差异有统计学意义(P<0.05)。经受试者工作特征曲线分析显示,血清Gal-3、CysC水平均能预测AMI患者PCI术后AKI的发生,曲线下面积分别为0.854、0.917(P<0.05)。经多因素logistic回归分析显示,高血压、糖尿病、术前使用ACEI、血肌酐>100μmol·L^(-1)、Gal-3≥95.840 ng·L^(-1)、CysC≥1.330 mg·L^(-1)为AMI患者PCI术后发生AKI的危险因素(P<0.05)。结论急诊PCI术前检测血清Gal-3、CysC水平对AMI患者术后AKI的发生有较高的预测价值,且敏感度和特异度均较高。

外源性GDF11通过调控LOX-1介导的内质网应激途径改善高血压大鼠血管重构

[目的]探讨外源性生长分化因子11(GDF11)通过调控凝集素样氧化型低密度脂蛋白受体1(LOX-1)介导的内质网应激途径对高血压大鼠血管重构的影响。[方法]将大鼠随机分为对照组、模型组(AngⅡ诱导的高血压大鼠模型组)、r-GDF11组、Ab-LOX-1组和r-GDF11+r-LOX-1组,每组10只。采用动物无创血压仪检测大鼠尾动脉血压变化;HE染色评估主动脉血管形态;Masson染色评估主动脉组织胶原沉积情况;Western blot检测主动脉组织中GDF11、LOX-1及内质网应激相关蛋白表达。[结果]与对照组比较,模型组大鼠血压升高,主动脉组织中膜厚度(MT)、MT/管腔内径(LD)比值增大,LD减小(均P<0.05);主动脉组织胶原纤维容积分数(CVF)值、葡萄糖调节蛋白78(GRP78)和转录激活因子6(ATF6)蛋白表达、磷酸化PKR样内质网调节激酶(p-PERK)/PERK和磷酸化肌醇需求酶1α(p-IRE1α)/IRE1α比值均升高(均P<0.05)。与模型组比较,r-GDF11组和Ab-LOX-1组大鼠血压降低,主动脉组织MT、MT/LD均减小,LD增大(均P<0.05);主动脉组织CVF值、GRP78和ATF6蛋白表达、p-PERK/PERK和p-IRE1α/IRE1α比值均显著降低(均P<0.05)。与r-GDF11组比较,r-GDF11+r-LOX-1组大鼠血压升高,主动脉组织MT、MT/LD增大,LD减小(均P<0.05);主动脉组织CVF值、GRP78和ATF6蛋白表达、p-PERK/PERK和p-IRE1α/IRE1α比值显著升高(均P<0.05)。[结论]外源性GDF11通过抑制LOX-1介导的内质网应激途径改善高血压大鼠血管重构。

唾液酸结合免疫球蛋白样凝集素-15在食管鳞癌组织中的表达及意义

目的探讨免疫检查点唾液酸结合免疫球蛋白样凝集素-15(Siglec-15)在食管鳞癌中的表达及意义。方法免疫组化检测食管鳞癌组织、癌旁正常食管组织中Siglec-15表达情况,并比较分析其与食管鳞癌患者临床病理特征的关系。结果Siglec-15在食管鳞癌组织中表达阳性率为60.0%(48/80),高于癌旁正常食管组织中的23.75%(19/80)(χ^(2)=21.595,P<0.001)。食管鳞癌组织中Siglec-15蛋白表达与患者肿瘤直径、T分期、TNM分期、N分期及分化程度有关(χ^(2)=7.500,P=0.006;χ^(2)=10.342,P=0.001;χ^(2)=22.547,P=0.016;χ^(2)=20.508,P<0.001;χ^(2)=12.586,P=0.002)。结论Siglec-15在食管鳞癌组织中高表达,与患者的疾病进展显著相关。