A novel matrix metalloproteinase (MMP) was identified from leukocytes and found to be specifically expressedby peripheral blood leukocytes among 29 different tissuesexamined. Named leukolysin, it encodes for 562 resid...A novel matrix metalloproteinase (MMP) was identified from leukocytes and found to be specifically expressedby peripheral blood leukocytes among 29 different tissuesexamined. Named leukolysin, it encodes for 562 residueswith a conserved MMP structure, i.e., pre-, pro-, catalytic , hinge- and hemopexin-like domains, but also a RXK/RRmotif, known for its role in MMP zymogen activation, anda C-terminal hydrophobic segment. Overall, leukolysindisplays the strongest homology to the newly identifiedMT-MMP subgroup with 45% and 39% identities to MT4and MT1-MMPs vs 30% and 31.5% to MMP1 and 3 respectively. Unlike MT4-MMP whose proteolytic activityremains undefined, a C-terminally truncated leukolysin isexpressed as a strong gelatinolytic species at 28 kDa whichis derived from a cell-associated 34 kDa proenzyme, presumably by furin or proprotein convertase mediated removal of the propeptide (-6 kDa). By green fluorescentprotein (GFP) tagging, the intracellular proenzyme is localized to granules throughout the cell, suggesting thatactivation occur immediately prior to secretion. Taken together, leukolysin may be part of the proteolytic arsenaldeployed by leukocytes during inflammatory responses.展开更多
This work investigated the land-use/land-cover and some physico-chemical properties of the soils of Mt Cameroon and presented same in maps. ArcGIS Pro mapping software, Landsat images, Global Positioning Systems (GPS)...This work investigated the land-use/land-cover and some physico-chemical properties of the soils of Mt Cameroon and presented same in maps. ArcGIS Pro mapping software, Landsat images, Global Positioning Systems (GPS) coordinates collected from the field combined with updated shape files from competent services were used to produce the location and land-use/land-cover maps. Sixteen topsoil samples (0 - 20 cm) were collected, 4 from each land use/cover category: farmland, forest, plantation and settlement, and analysed for soil pH, cation exchange capacity (CEC), bulk density, moisture content and soil texture, in the laboratory using standard analytical procedures. This data was used to produce spatial distribution maps using ordinary kriging, in ArcGIS Pro. The main terrestrial land use/cover categories comprised of the forest (mangrove, lowland, montane and sub-montane), agroforestry, plantations, grassland, settlement, cropland, shrubby savannah, and bare lava. Bulk density showed the highest values in settlement areas and least values under forest land-use categories. Soil moisture content exhibited a reverse trend compared to that of soil bulk density. Forest soils were the sandiest while soils in plantation agricultural land were the most clayey. The soils were slightly acidic to neutral with soils from agricultural land being more acidic (pH<sub>(water)</sub> = 5.43). It is discernible from the results that the conversion from forest to other land use/cover classes enhances soil degradation and that soil physico-chemical properties adequately serve as indicators of soil quality in the Mt Cameroon area.展开更多
多团队系统理论研究是当前管理学、心理学、工程、医学领域的研究热点,理论的提出到现在只有19年时间,但在组织管理中的重要作用已得到学者们的认同。当前多团队系统理论研究呈现碎片化特征,缺乏对近年文献的系统梳理,难以为后续研究提...多团队系统理论研究是当前管理学、心理学、工程、医学领域的研究热点,理论的提出到现在只有19年时间,但在组织管理中的重要作用已得到学者们的认同。当前多团队系统理论研究呈现碎片化特征,缺乏对近年文献的系统梳理,难以为后续研究提供清晰的方向。采用知识图谱分析法,以Web of Science数据库2001—2018年收录的171篇多团队系统理论文献为数据来源,探讨了多团队系统领域的研究现状,提炼了该研究主题的演进轨迹和前沿热点。研究表明,多团队系统理论研究在国内外学术界正在形成利用多层次、多阶段的视角尝试解决实际管理问题的趋势。展开更多
文摘A novel matrix metalloproteinase (MMP) was identified from leukocytes and found to be specifically expressedby peripheral blood leukocytes among 29 different tissuesexamined. Named leukolysin, it encodes for 562 residueswith a conserved MMP structure, i.e., pre-, pro-, catalytic , hinge- and hemopexin-like domains, but also a RXK/RRmotif, known for its role in MMP zymogen activation, anda C-terminal hydrophobic segment. Overall, leukolysindisplays the strongest homology to the newly identifiedMT-MMP subgroup with 45% and 39% identities to MT4and MT1-MMPs vs 30% and 31.5% to MMP1 and 3 respectively. Unlike MT4-MMP whose proteolytic activityremains undefined, a C-terminally truncated leukolysin isexpressed as a strong gelatinolytic species at 28 kDa whichis derived from a cell-associated 34 kDa proenzyme, presumably by furin or proprotein convertase mediated removal of the propeptide (-6 kDa). By green fluorescentprotein (GFP) tagging, the intracellular proenzyme is localized to granules throughout the cell, suggesting thatactivation occur immediately prior to secretion. Taken together, leukolysin may be part of the proteolytic arsenaldeployed by leukocytes during inflammatory responses.
文摘This work investigated the land-use/land-cover and some physico-chemical properties of the soils of Mt Cameroon and presented same in maps. ArcGIS Pro mapping software, Landsat images, Global Positioning Systems (GPS) coordinates collected from the field combined with updated shape files from competent services were used to produce the location and land-use/land-cover maps. Sixteen topsoil samples (0 - 20 cm) were collected, 4 from each land use/cover category: farmland, forest, plantation and settlement, and analysed for soil pH, cation exchange capacity (CEC), bulk density, moisture content and soil texture, in the laboratory using standard analytical procedures. This data was used to produce spatial distribution maps using ordinary kriging, in ArcGIS Pro. The main terrestrial land use/cover categories comprised of the forest (mangrove, lowland, montane and sub-montane), agroforestry, plantations, grassland, settlement, cropland, shrubby savannah, and bare lava. Bulk density showed the highest values in settlement areas and least values under forest land-use categories. Soil moisture content exhibited a reverse trend compared to that of soil bulk density. Forest soils were the sandiest while soils in plantation agricultural land were the most clayey. The soils were slightly acidic to neutral with soils from agricultural land being more acidic (pH<sub>(water)</sub> = 5.43). It is discernible from the results that the conversion from forest to other land use/cover classes enhances soil degradation and that soil physico-chemical properties adequately serve as indicators of soil quality in the Mt Cameroon area.
文摘多团队系统理论研究是当前管理学、心理学、工程、医学领域的研究热点,理论的提出到现在只有19年时间,但在组织管理中的重要作用已得到学者们的认同。当前多团队系统理论研究呈现碎片化特征,缺乏对近年文献的系统梳理,难以为后续研究提供清晰的方向。采用知识图谱分析法,以Web of Science数据库2001—2018年收录的171篇多团队系统理论文献为数据来源,探讨了多团队系统领域的研究现状,提炼了该研究主题的演进轨迹和前沿热点。研究表明,多团队系统理论研究在国内外学术界正在形成利用多层次、多阶段的视角尝试解决实际管理问题的趋势。
