QTL Identification of the Insensitive Response to Photoperiod and Temperature in Soybean by Association Mapping

The insensitive response to photoperiod and temperature is an important quantitative trait for soybean in wide adaptation breeding. The natural variation in response to photoperiod and temperature was detected using 275 accessions of soybean [Glycine max （L.） Merrill] from China. Genome-wide association mapping, based on population structure analysis, was carried out using 118 SSR markers by the TASSEL GLM （general linear model） program. Nine SSR markers （P〈0.01） were associated with the value of the response to photoperiod and temperature （VRPT） caused by days to flowering （DF）, among which, Satt308 （LG M）, Sattl50 （LG M） and Satt440 （LG l）, were identified in both 2006 and 2007. Twelve SSR markers （P〈0.01） were associated with VRPT caused by days to maturity （DM）, among which three markers, Satt387 （LG N）, Satt307 （LG C2） and AW310961 （LG J）, were detected in both 2006 and 2007. In addition, a total of 20 elite alleles were screened out over 2006 and 2007 for being associated with an insensitive response to photoperiod and temperature （IRPT） caused by DF and a total of seven different elite alleles were screened out for being associated with IRPT caused by DM. Among these elite alleles, five alleles, Satt150-244, Satt308-164, Satt308-206, Satt440-176, and Satt440-206, were associated with IRPT caused by DF and were identified in both years, but only one allele, Satt307-170, was identified as being associated with an IRPT caused by DM. Based on these elite alleles, a set of typical accessions were screened out. The result about the genetic basis of IRPT is meaningful for soybean wide adaption breeding.

Prepulse inhibition (PPI) of tactile startle response in recombinant congenic strains of mice:QTL mapping and comparison with acoustic PPI

Prepulse inhibition （PPI） of the startle response is a psychophysiological measure of sensorimotor gating believed to be cross-modal between different sensory systems. We analyzed the tactile startle response （TSR） and PPI of TSR （tPPI）, using light as a prepulse stimulus, in the mouse strains A/J and C57BL/6J and 36 recombinant congenic strains derived from them. Parental strains were significantly different for TSR, but were comparable for tPPI. Among the congenic strains, variation for TSR was significant in both genetic backgrounds, but that of tPPI was significant only for the C57BL/6J background. Provisional mapping for loci modulating TSR and tPPI was carried out. Using mapping data from our previous study on acoustic startle responses （ASR） and PPI of ASR （aPPI）, no common markers for aPPI and tPPI were identified. However, some markers were significantly associated with both ASR and TSR, at least in one genetic background. These results indicate cross-modal genetic regulation for the startle response but not for PPI, in these mouse strains.

Stochastic response analysis of noisy system with non-negative real-power restoring force by generalized cell mapping method

The stochastic response of a noisy system with non-negative restoring force is investigated. The generalized cell mapping （GCM） method compute the transient and stationary probability density functions （PDFs） real-power is used to Combined with the global properties of the noise-free system, the evolutionary process of the tran- sient PDFs is revealed. The results show that stochastic P-bifurcation occurs when the system parameter varies in the response analysis and the stationary PDF evolves from bimodal to unimodal along the unstable manifold during the bifurcation.

基于ADO.NET的O/R Mapping中间件的研究

目前大多数软件开发都采用面向对象技术,而数据仍然保存在关系数据库中。由于对象模型和关系模型存在阻抗不匹配,因此实现对象与关系的映射已成为软件开发领域的关键问题。采用O/R M app ing(对象/关系映射)技术、XM L技术和软件分层的设计思想,实现了一个有效的数据库访问中间件,解决了利用ADO.NET开发应用程序遇到的数据表示和存取问题。

Simultaneous Localization and Mapping System Based on Labels

In this paper a label-based simultaneous localization and mapping（ SLAM） system is proposed to provide localization to indoor autonomous robots. In the system quick response（ QR） codes encoded with serial numbers are utilized as labels. These labels are captured by two webcams,then the distances and angles between the labels and webcams are computed. Motion estimated from the two rear wheel encoders is adjusted by observing QR codes. Our system uses the extended Kalman filter（ EKF） for the back-end state estimation. The number of deployed labels controls the state estimation dimension. The label-based EKF-SLAM system eliminates complicated processes,such as data association and loop closure detection in traditional feature-based visual SLAM systems. Our experiments include software-simulation and robot-platform test in a real environment. Results demonstrate that the system has the capability of correcting accumulated errors of dead reckoning and therefore has the advantage of superior precision.

Dynamic Analysis of Stochastic Friction Systems Using the Generalized Cell Mapping Method

Friction systems are a kind of typical non-linear dynamical systems in the actual engineering and often generate abundant dynamics phenomena.Because of non-smooth characteristics,it is difficult to handle these systems by conventional analysis methods directly.At the same time,random perturbation often affects friction systems and makes these systems more complicated.In this context,we investigate the steady-state stochastic responses and stochastic P-bifurcation of friction systems under random excitations in this paper.And in order to retain the non-smooth of friction system,the generalized cell mapping(GCM)method is first used to the original stochastic friction systems without any approximate transformation.To verify the accuracy and validate the applicability of the suggested approach,we present two classical nonlinear friction systems,i.e.,Coulomb force model and Dahl force model as examples.Meanwhile,this method is in good agreement with the Monte Carlo simulation method and the computation time is greatly reduced.In addition,further discussion finds that the adjustable parameters can induce the stochastic P-bifurcation in the two examples,respectively.The stochastic P-bifurcation phenomena indicate that the stability of the friction system changes very sensitively with the parameters.Research of responses analysis and stochastic P-bifurcation has certain significances for the reliability and stability analysis of practical engineering.

Genetic analysis and QTL mapping of growth period traits and plant height traits in soybean recombinant inbred lines from Dongnong 47 × PI 317334-B

High and stable yield is the main goal of soybean genetic improvement.In this study,association analysis was used to detect the quantitative trait loci(QTL)for the plant height,and soybean growth period using 182 SSR markers in the RIL population of 136 F_(4:8) lines,which developed from a cross between photoperiod-insensitive cultivar‘Dongnong 47’and photoperiod-sensitive variety PI317334–B.The results showed that 33 QTLs related to soybean growth period and plant height traits were detected by compound interval mapping,and were located on 12 linkage groups including N,C1,C2,J,D1a,B2,E,G,A2,O,L,I,with the contribution rate of 7.85–33.84%.These QTL loci and linkage markers related to soybean photoperiod sensitivity,would be helpful to identify key genes that control soybean photoperiod sensitivity,and provide an important basis for the breeding of new photoperiod-insensitive soybean varieties based on molecular design breeding.

Slam Technology on Disaster Response

Disaster response demands rapid, accurate, and comprehensive situational awareness. This study explores the potential of Simultaneous Localization and Mapping (SLAM) technology to enhance disaster response efforts. By integrating sensors and algorithms, SLAM enables autonomous systems to create detailed maps of disaster-stricken areas while simultaneously determining their own location. This research investigates the application of SLAM in various disaster scenarios, assesses its performance in challenging conditions, and analyzes its potential to improve search and rescue, damage assessment, and resource allocation. The findings highlight the advantages of SLAM in providing real-time data, supporting decision-making, and increasing the efficiency of disaster response operations.

Mapping of Defense Response Gene Homologs and Their Association with Resistance Loci in Maize

Defense response genes in higher plant species are involved in a variety of signal transduction pathways and biochemical reactions to counterattack invading pathogens. In this study, a total of 366 non-redundant defense response gene homologs （DRHs）, including 124 unigenes/expressed sequence tags, 226 tentative consensuses, and 16 DRH contigs have been identified by mining the Maize Genetics and Genomics and The Institute for Genomic Research maize databases using 35 essential defense response genes. Of 366 DRHs, 202 are mapped to 152 loci across ten maize chromosomes via both the genetic and in silico mapping approaches. The mapped DRHs seem to cluster together rather than be evenly distributed along the maize genome. Approximately half of these DHRs are located in regions harboring either major resistance genes or quantitative trait loci （QTL）. Therefore, this comprehensive DRH linkage map will provide reference sequences to identify either positional candidate genes for resistance genes and/or QTLs or to develop makers for fine-mapping and marker-assisted selection of resistance genes and/or QTLs.

Evaluation of 30 DNA damage response and 6 mismatch repair gene mutations as biomarkers for immunotherapy outcomes across multiple solid tumor types

Objective:DNA damage response(DDR)genes have low mutation rates,which may restrict their clinical applications in predicting the outcomes of immune checkpoint inhibitor(ICI)treatment.Thus,a systemic analysis of multiple DDR genes is needed to identify potential biomarkers of ICI efficacy.Methods:A total of 39,631 patients with mutation data were selected from the cBioPortal database.A total of 155 patients with mutation data were obtained from the Fudan University Shanghai Cancer Center(FUSCC).A total of 1,660 patients from the MSK-IMPACT cohort who underwent ICI treatment were selected for survival analysis.A total of 249 patients who underwent ICI treatment from the Dana-Farber Cancer Institute(DFCI)cohort were obtained from a published dataset.The Cancer Genome Atlas(TCGA)level 3 RNA-Seq version 2 RSEM data for gastric cancer were downloaded from cBioPortal.Results:Six MMR and 30 DDR genes were included in this study.Six MMR and 20 DDR gene mutations were found to predict the therapeutic efficacy of ICI,and most of them predicted the therapeutic efficacy of ICI,in a manner dependent on TMB,except for 4 combined DDR gene mutations,which were associated with the therapeutic efficacy of ICI independently of the TMB.Single MMR/DDR genes showed low mutation rates;however,the mutation rate of all the MMR/DDR genes associated with the therapeutic efficacy of ICI was relatively high,reaching 10%–30%in several cancer types.Conclusions:Coanalysis of multiple MMR/DDR mutations aids in selecting patients who are potential candidates for immunotherapy.

Mismatch repair protein expression and intratumoral budding in rectal cancer are associated with an increased pathological complete response to preoperative chemoradiotherapy: A case-control study

AIM To determine whether the association of rectal adenocarcinoma with a defective-mismatch repair system(dMMR) was associated with a pathological complete response(pCR) to preoperative chemoradiotherapy.METHODS A case-control study was designed with the aim of determining if patients with rectal adenocarcinoma with dM MR had an associated high pCR rate in response to neoadjuvant chemoradiotherapy(nCRT).RESULTS Seventy-two cases with pCR were compared against 144 controls without pCR. Across 216 cases, the mean age was 56.8 years, 140(64.8%) were men, and 63(29.2%) demonstrated the dMMR system. The pCR was associated with G1 tumors, dMMR, the absence of vascular invasion, and low tumor budding in the pretreatment biopsy. In a multivariant analysis, the factors associated with pCR were dMMR(OR: 2.61; 95%CI: 1.355-5.040, P = 0.004) and a low degree of tumor budding(OR: 2.52; 95%CI: 1.366-4.894, P = 0.025).CONCLUSION We found an independent association between dMMR and a low rate of tumor budding, with a higher rate of pCR, in the basal biopsies of patients with rectal carcinoma subjected to nCRT.

Tumor recurrence after pathological complete response in locally advanced gastric cancer after neoadjuvant therapy:Two case reports

BACKGROUND The pathological complete response(ypCR)rate following neoadjuvant chemotherapy for advanced gastric cancer remains low and lacks a universally accepted treatment protocol.Immunotherapy has achieved breakthrough progress.CASE SUMMARY We report two female patients with gastric cancer defined as clinical stage cT4N1-2M0.Detection of mismatch repair protein showed mismatch repair function defect,and perioperative treatment with programmed death protein 1 inhibitor combined with S-1+oxaliplatin achieved ypCR.Surprisingly,the patients underwent clinical observation after surgery but developed different degrees of metastasis at~6 mo after surgery.CONCLUSION PD-1 inhibitor combined with chemotherapy provides a more strategic choice for comprehensive perioperative treatment of gastric cancer.

概念图在护理实习生应急能力培训中的应用

目的运用概念图开展护理实习生应急能力培训,为临床实践教学及相关研究提供参考。方法根据需求对某三甲医院护理实习生进行应急能力培训,培训采用传统教学和概念图教学两种方式,对培训前后两届学生的应急能力进行调查和比较。结果护理实习生应急能力处于中低水平。培训后,护理实习生的应急能力均有所提升(P<0.05),但与传统教学模式相比,概念图教学培训后护理实习生的应急能力提升更明显(P<0.05)。结论应用概念图教学可有效提升护理实习生的应急能力的培训效果。教学医院可以采用多种方式教学,优化教学形式,提高护理实习生应急能力。

江西宜丰县2012—2022年短时强降水的雷达回波特征分析

为了做好宜丰县短时强降水“1小时风险叫应”标准研究,使用宜丰雨量、江西雷达拼图等资料,采用统计学、雷达气象学等原理和方法,对宜丰县2012—2022年短时强降水进行分析,结果表明:1)2012—2022年18个气象观测站共出现短时强降水273站次。最多的是板坑水库(19次),最少的是双峰林场(8次);2)在大片絮状回波带中(层状云与积状云混合),存有大于45 dBZ的短带回波,在短带回波覆盖地方,多处会出现短时强降水。絮状回波带大于45 dBZ的短带回波面积都在500 km^(2)以上;3)块状单体回波尺度较小,基本孤立存在,回波强度CR在50~60 dBZ。有时几个块状单体回波聚在一起,但之间有间隔,有时块状单体回波存在于大范围絮状回波团中。块状单体回波在小时平均回波图上的反映差距较大;4)雷暴回波群的45 dBZ回波面积都≥100 km^(2),这个指标为识别短时强降水提供量化阈值。研究结果对宜丰短时强降水“1小时风险叫应”研究具有指导意义。

Identification of prior candidate genes for Sclerotinia local resistance in Brassica napus using Arabidopsis cDNA microarray and Brassica-Arabidopsis comparative mapping

Arabidopsis cDNA arrays were used to screen the local-defense-associated genes in oilseed rape(Brassica napus L.)at the challenge of Sclerotinia sclerotiorum.61 genes with two-fold expression changes were screened out from the local tissue around the necrosis.Among them,36 unique genes were up-regulated and 25 unique genes were down-regulated.RT-PCR and Northern blot results were consistent with the array results,suggesting Arabidopsis arrays were useful for transcriptional profiling of B.napus genes.Some of these genes were located in the interval of some QTLs for Sclerotinia resistance in B.napus by Brassica-Arabi-dopsis comparative mapping.These genes may have priority to be pursued for more intensive research.

无先兆性偏头痛患者力敏腧穴分布规律及红外特征研究

目的:观察无先兆性偏头痛(MO)患者和健康受试者力敏腧穴体表分布情况,分析MO患者力敏腧穴分布规律及红外温度特征。方法:选取2019年12月至2022年3月就诊于江西中医药大学附属医院的60例MO患者和60例健康受试者作为研究对象,对120例研究对象进行体表力敏腧穴探查并记录,分析总结MO患者力敏腧穴分布与经脉、神经节段的位置关系;运用红外热成像技术(TTM)观察总结MO患者力敏腧穴红外特征。结果:MO组体表力敏腧穴出现率为88.3%(53/60),健康组为6.67%(4/60,P<0.01)。MO组力敏腧穴主要分布于胆经、肝经以及三焦经,率谷、风池、太冲穴区敏化率较高。MO组力敏腧穴多分布于三叉神经、C_(2)、C_(7)神经节段。力敏腧穴红外温度及部位特征表现为:MO患侧>MO健侧>健康受试者,MO患者颞部、目额部、后头部、前臂外侧、足背部力敏腧穴存在明显不规则热源分布。结论:MO患者力敏腧穴与传统经脉、神经节段位置密切相关,红外温度升高、体表不规则红外热源分布是其重要特征。

Molecular cloning, expression analysis and chromosomal mapping of salt-responsive cDNAs in rice (Oryza sativa L.)

By using differential display PCR (DD-PCR) technique, two salt-inducible and one salt-repressed cDNA fragments were isolated from rice. The three cDNA fragments were characterized respectively as partial sequence of rice S-adenosylmethionine decarboxylase (SAMDC) gene, a new member of translation elongation factor lA gene (named REF1A), and a novel gene whose function is unknown (named SRG1). The full-length cDNA of SAMDC gene (named SAMDC1) was further isolated by RT-PCR approach and the deduced polypeptide was found to be homologous to SAMDC proteins of other plants, yeast and human. Northern hybridization revealed that expression of SAMDC1 and REF1A was induced, while SRG1 was dramatically repressed, by salinity stress. Southern blot analysis demonstrated that SAMDC 1 and SRG1 were present as a single copy gene in rice genome, whereas rice REF1A gene was organized as a gene family. The REF1A, SAMDC1, and SRG1 genes were located on chromosome 3,4, and 6 respectively by RFLP mapping approach using ZYQ8/JX17 DH population and RFLP linkage maps.

价值流程图优化在静脉曲张术前准备中的应用

目的:探讨价值流程图优化在静脉曲张术前准备中的应用效果。方法:选择2022年3月—2023年6月收治的静脉曲张手术患者96例,按照随机数字表法分为观察组和对照组,每组48例。对照组采用围术期常规护理措施,观察组在对照组基础上加用基于价值流程图优化的术前准备流程干预。对比两组围术期指标(术前准备时间、术中停顿时间)、干预前后应对方式[医学应对方式问卷(MCMQ)]、心理应激反应[症状自评量表(SCL-90)]评分以及护理满意度[纽卡斯尔护理满意度量表(NSNS)]。结果:观察组术前准备时间、术中停顿时间均低于对照组(P<0.05)。干预后,两组MCMQ中面对维度评分均升高(P<0.05),且观察组高于对照组(P<0.05);两组MCMQ中回避、屈服维度评分和SCL-90中躯体化、强迫、人际关系敏感、抑郁、焦虑、敌对维度评分均降低,且观察组低于对照组(P<0.05);观察组护理总满意度高于对照组(P<0.05)。结论:将基于价值流程图优化的术前准备流程用于静脉曲张手术前准备中,可缩短术前准备和术中停顿时间,减轻患者心理应激反应,提升其对护理工作的满意度。

风-光-储和需求响应协同的虚拟电厂日前经济调度优化

目前可再生能源直接并入电网仍然面临稳定性和经济性问题,经过虚拟电厂整合可以缓解对电网的影响。以系统整合后最终运行成本达到最小作为目标,进行新能源出力和负荷在未来24 h的预测,计及电网侧在不同时间内的电价变化情况,采用反向学习的混沌映射自适应粒子群算法对风-光-储能和需求响应不同组合搭配的5种调度方案进行探讨,与原始粒子群算法相比,所提算法可以跳出局部最优解而找到全局最优解。计算结果表明,风-光-储和需求响应都参与供电相比风-光-储供电可以将运行成本降低4.47%,用户舒适度提高3.51%。

基于混沌映射的抗机器学习攻击强物理不可克隆函数

物理不可克隆函数(PUF)在硬件安全领域具有广阔的应用前景,然而易受到基于机器学习等建模攻击。通过对强PUF电路结构和混沌映射机理的研究,该文提出一种可有效抵御机器学习建模攻击的PUF电路。该电路将原始激励作为混沌映射初始值,利用PUF激励响应映射时间与混沌算法迭代深度之间的内在联系产生不可预测的混沌值,并采用PUF中间响应反馈加密激励,进一步提升激励与响应映射的复杂度,增强PUF的抗机器学习攻击能力。该PUF采用Artix-7 FPGA实现,测试结果表明,即使选用的激励响应对数量高达106组,基于逻辑回归、支持向量机和人工神经网络的攻击预测率仍接近50%的理想值,并具有良好的随机性、唯一性和稳定性。