固定化脂肪酶ANL-MARE催化酸解合成1-油酸-2-棕榈酸-3-亚油酸甘油三酯

1-油酸-2-棕榈酸-3-亚油酸甘油三酯(OPL)是人乳中主要的脂质组成,为满足婴幼儿配方食品母乳化需求,该试验探究了新型固定化脂肪酶ANL-MARE催化制备OPL的方法。试验成功制备和表征了固定化酶ANLMARE,并以ANL-MARE为生物催化剂,建立了一种用三棕榈酸甘油三酯(PPP)、油酸(OA)和亚油酸(LA)高效酶催化制备富含OPL结构脂的方法。经单因素和响应面试验优化,获得OPL最优合成工艺为:PPP与总脂肪酸的摩尔比1:14.27,OA与LA摩尔比为1:0.76,脂肪酶添加量12.70%,反应温度50℃,反应4 h,此条件下产物中OPL相对含量为47.93%,2位棕榈酸(sn-2 PA)占总棕榈酸(PA)的质量分数(sn-2 PA相对含量)为71.69%。此外,固定化酶ANL-MARE与商业脂肪酶相比,表现出较好的催化合成OPL的活性。综上所述,固定化酶ANL-MARE具有催化制备OPL结构脂的重大潜力,为人乳脂替代脂的高效制备提供了新策略和理论基础。

Z箍缩内爆的MARED程序1维模拟分析

研制可靠的数值模拟工具对Z箍缩内爆产生X光辐射过程进行理论研究、实验分析以及负载设计至关重要。介绍了2维三温辐射磁流体力学程序(MARED)的物理方案,给出了MARED程序的1维检验结果,验证表明MARED程序适用不同装置条件、不同负载参数。结合丝阵Z箍缩实验的数值模拟和分析表明:相同负载质量条件下,钨丝阵内爆产生的X光辐射功率远大于铝丝阵产生的X光功率;相同负载电流条件下,负载质量越大,计算得到X光功率越低;X光功率随着负载电流增加而增加。

A comparative evaluation of methicillin-resistant staphylococci isolated from harness racing-horses,breeding mares and riding-horses in Italy

Objective:To investigate the prevalence of methicillin-resistant staphylococci(MRS)which is a potencial risk factor of transmission between animals and humans in different types of horses(harness racing-horses,breeding mares and riding-horses)and to compare the antimicrobial resistance of the isolates.Methods:A total of 191 healthy horses,housed at different locations of the Campania Region(Italy),were included in the study.Nasal swab samples were collected from each nostril of the horses.The mecA gene was detected by a nested PCR technique.Antibiotic susceptibility was tested for each isolate.Results:MRS was isolated from nasal samples of 68/191(35.6%;95%CI:28.9%-42.9%)healthy horses.All isolates were coagulase-negative with the exception of two coagulase-positive MRS strains,identified as Staphylococcus aureus and Staphylococcus pseudintermedius.2/83(2.4%;95%,CI:0.4%-9.2%).Interestingly,both coagulase-positive MRS isolates were from harness racing-horses.These horses also presented a significantly higher positivity for MRS(53.3%;95%CI:40.1%-66.1%)than the breeding mares and riding-horses groups.Antibiotic susceptibility testing showed difference between isolates due to different origins except for an almost common high resistance to aminopenicillins,such as ampicillin and amoxicillin.Conclusions:It can be concluded that harness racing-horses may act as a significant reservoir of MRS as compared to breeding mares and riding-horses.

Estimation of the elastic thickness over ancient Mare Moscoviense

The Moscoviense basin is an atypical lunar impact basin with concentric rings of positive and negative gravity anomalies. This basin can provide insights into the inhomogeneous thermal activities across the farside of the Moon. Based on an updated spherical harmonic thin elastic-shell loading model, we used localized admittance analyses to estimate the elastic thickness as well as other associated selenophysical parameters for the Moscoviense basin. The high precision gravity and topography data employed in our estimation were collected by the Gravity Recovery and Interior Laboratory and the Lunar Orbiter Laser Altimeter missions. Our results indicate that the crust-mantle interface is mainly compensated by the prefilling depth rather than the observed surface topography. The results constrained within two standard deviations yielded a small load ratio(~0.168), a best-fit crustal thickness of 36.2 km, and an optimized crustal density of 3159.5 kg m-3. Such large density approaches the density of olivine-rich mantle materials, implying that the excavation of the Mare Moscoviense occurred during a basin-forming impact. The inversed elastic thickness at Mare Moscoviense was around 18 km, lower than the previous results(~60 km) found over Mare basins on the lunar nearside. These results indicate that extreme thermal activity existed during the Moscoviense basin-forming period such as reheating mechanisms from a double-impact process and mare volcanism.

Time of foaling in Arabian mares raised in Tiaret, Algeria

Objective:To enhance effectiveness of reproduction management in Arabian mares,factorsinfluencing the time of foaling were investigated in this study.Methods:Data were collectedat the National Haras of Tiaret in Algeria from 2003 to 2010.The foaling time of 255 Arabian purebred mares,aged from 3 to 20 years were used for this study.Results:A total of 78.07%of foalinghappens between 7 pm and 6 am.Conclusions:The influence of the month of foaling and the sexof the foal,on the time of foaling was statically significant.

Two Lunar Mare Soil Simulants

Two new lunar mare soil simulants,NAO-2 and NAO-3,have been created in National Astronomical Observatories(NAO),Chinese Academy of Sciences.These two simulants were produced from low-titanium basalt and high-titanium basalt respectively.The chemical composition,mineralogy, particle size distribution,density,angle of internal friction,and cohesion of both simulants have been analyzed,indicating that some characteristics of NAO-2 and NAO-3 are similar to those of Apollo 14 and Apollo 11 landing site soils.NAO-2 and NAO-3 will be of great benefit to the scientific and engineering research on lunar soil.

Endoscopic monitoring the estrous phase of Arabian mares with assaying its biopsy

Objective:To evaluate the changes occur in the vagina, cervix and endometrium during the pre- ovulatory period in Arabian mares by using endoscope as a diagnostic tool as well as the availability of taking an endoscopic biopsy during this stage.Methods:Ten cyclic and healthy arabian mares were subjected to ultrasound scanning of their ovaries to estimate the diameter of the preovulatory follicles, collection of blood samples for estimating the hormonal levels (Estradiol-17β and progestrone) for confirming the estrous phase, endoscopic monitoring their vagina, cervix and endometrium, the availability of taking biopsical samples and histological examination of the obtained samples.Results:The obtained data revealed that, the mean diameter of the preovulatory follicles was (3.95±0.34) cm, the plasma estradiol and progestrone levels were (85.98±5.26) pg/mL and (0.73±0.23) ng/mL, respectively, endoscopic monitoring the vagina, cervix and endometrium revealed hyperemic vaginal mucous membrane, soft and opened cervical ostium with abundant estrous mucous and folding, hyperemic and edematous appearance of the endometrium, respectively and the histological features of the biopsical endometrial samples revealed that, The lamina propria-sub mucosa was packed with simple or branched tubular glands lined with simple columnar epithelium showing oval or rounded dark nuclei with different degrees of secretory activities of the glands.Conclusions: The endoscope proved to be a reliable tool for confirming the estrous phase in mares and monitoring the gross features of the vagina, the cervix and the endometrium, in addition to the availability of taking biopsical smears.

Endometritis associated with Enterococcus casseliflavus in a mare:A case report

Infectious endometritis is one of the main causes of subfertility/infertility in the mare. In this report, we present the first case of endometritis in mare associated with a strain of Enterococcus casseliflavus, an unusual gram-positive bacterium which can also be a zoonotic agent. Furthermore, the isolated strain showed a worrying multidrug-resistant profile. The accurate finding of a successful antimicrobial treatment and consequently,the pregnancy diagnosis indicate the importance to isolate, identify and define the antibiotic resistance profile of bacteria associated with endometritis.

A novel strategy to extract lunar mare KREEP-rich metal resources using a silicon collector

The lunar mare potassium(K)-,rare-earth elements(REEs)-and phosphorous(P)-rich(KREEP-rich) region is a unique late-stage product of magma crystallization,in which ilmenite and incompatible elements have high grades,thus forming a giant natural reservoir.The extraction and purification of the high-value metal resources in the KREEP-rich region not only meet the construction needs of the lunar base but also solve the problem of resource scarcity on Earth.In this study,photovoltaic elemental silicon(Si) was used as a collector to extract ilmenite resources,REEs,and nuclear energy elements from basalt in the lunar mare KREEP-rich region at 1873 K.Based on experimentation,the metals titanium(Ti)and iron(Fe) in the lunar mare ilmenite are found to be enriched and solidified in the form of Si-based alloys.The contents of valuable incompatible elements in the KREEP-rich area are also found to be enriched and contained in the incompatible trace elements(ITEs) phase of the alloy.Among them,REEs(e.g.,cerium(Ce) and thulium(Tm)) and nuclear elements(e.g.,thorium(Th) and uranium(U)) are found to account for 82.61 wt% of the ITEs phase.This process provides a simple and feasible scheme for the insitu resource utilization(ISRU) of the lunar surface and is suitable for the extraction and enrichment of lunar metal resources.

Z箍缩内爆的MARED程序数值模拟分析

MARED程序是模拟Z箍缩内爆过程的二维三温辐射磁流体力学程序,它适用于不同装置条件和不同负载参数.利用MARED程序对Z箍缩内爆进行模拟,结合丝阵Z箍缩实验分析表明:相同负载质量条件下,钨丝阵内爆产生的X射线辐射功率远大于铝丝阵产生的X射线功率;相同负载电流条件下,负载质量越大,计算得到的X射线功率越低;X射线功率随着负载电流增加而增加.MARED程序能够较好地反映Z箍缩内爆动力学过程,特别是不稳定性发展的二维图像,能够给出与不稳定性简化模型的理论分析及实验结果定性一致的演化规律.MARED程序模拟丝阵填充泡沫形成辐射场的初步计算得到了与Sandia实验室模拟Z装置上丝阵填充泡沫定性一致的结果.

连翘叶提取物通过PXR/CYP17A1影响孕马血清促性腺激素在肝脏的代谢

【目的】研究连翘叶提取物(Forsythia suspensa leaves extract,FSLE)对孕马血清促性腺激素(pregnant mare serum gonadotropin,PMSG)在肝脏中代谢的影响,改善在畜牧生产中因PMSG代谢缓慢而造成的氧化应激。【方法】采用半仿生酶醇法提取连翘叶有效成分,给予昆明雌鼠不同含量的FSLE进行动物体内试验;收集小鼠血清,进行酶联免疫吸附试验,检测各组小鼠血清中PMSG的质量浓度;采集小鼠肝脏组织,检测氧化应激指标谷胱甘肽、超氧化物歧化酶、丙二醛和过氧化氢的水平;采用Western-blot检测小鼠肝脏组织孕烷X受体(pregnane X receptor,PXR)和细胞色素酶P45017A1(cytochrome P45017A1,CYP17A1)蛋白的表达水平。【结果】FSLE可显著降低小鼠血清中PMSG的质量浓度,且可以减缓PMSG代谢引起的氧化应激。Western-blot结果显示:FSLE可显著下调小鼠肝组织中PXR和CYP17A1蛋白的表达。【结论】连翘叶提取物可能是通过下调肝脏中PXR和CYP17A1的表达减缓PMSG代谢引起的氧化应激。

补喂赖氨酸和苏氨酸对放牧条件下泌乳母马血液生化指标的影响

通过给放牧条件下泌乳母马补饲赖氨酸和苏氨酸,探究其对泌乳母马血液生化指标的影响,为氨基酸调控泌乳期母马的健康提供参考依据。选择产驹日期相近(5月份),年龄7~9岁,胎次4~5胎,平均体重(428.00±33.42)kg,泌乳30 d的伊犁马母马24匹,随机分为4个组,每组6匹,分别为对照组、试验Ⅰ组、试验Ⅱ组和试验Ⅲ组。在相同的放牧条件下(放牧时间、饮水时间、挤奶时间和放牧草场完全相同),对照组不进行任何氨基酸补喂,试验Ⅰ组每匹马补饲赖氨酸40 g/d+苏氨酸20 g/d,试验Ⅱ组每匹马补饲赖氨酸60 g/d+苏氨酸40 g/d,试验Ⅲ组每匹马补饲赖氨酸80 g/d+苏氨酸60 g/d。整个补饲期为120 d,定期采集母马血液,测定相关生化指标。结果:在蛋白质代谢方面,试验Ⅰ~Ⅲ组母马血液中总蛋白和白蛋白含量均显著低于对照组(P<0.05),尿酸含量分别比对照组提高16.33%、37.20%和17.58%;在糖脂代谢方面,试验Ⅰ~Ⅲ组的总胆红素均极显著高于对照组(P<0.01),葡萄糖和甘油三酯含量均低于对照组;在酶活力方面,试验Ⅰ~Ⅲ组的谷丙转氨酶和谷草转氨酶活力均极显著低于对照组(P<0.01),碱性磷酸酶和肌酸激酶的活力随着赖氨酸和苏氨酸剂量的增加而呈上升趋势;在矿物质方面,Ca^(2+)、无机磷和Mg^(2+)的含量各组之间均无显著差异(P>0.05),但试验Ⅱ组Fe^(2+)含量最高,与其他3组有显著或极显著差异。综上,在放牧条件下给泌乳母马补饲赖氨酸和苏氨酸可降低其血液中葡萄糖、甘油三酯含量以及谷草转氨酶和谷丙转氨酶的活力,提高总胆红素、无机磷、Mg^(2+)和Fe^(2+)的含量,促进泌乳母马的健康,以每匹泌乳母马摄入60 g/d的赖氨酸和40 g/d的苏氨酸效果最佳。

SIMS Pb/Pb dating of Zr-rich minerals in lunar meteorites Miller Range 05035 and LaPaz Icefield 02224:Implications for the petrogenesis of mare basalt

Miller Range (MIL) 05035 and LaPaz Icefield (LAP) 02224 are unbrecciated lunar basalt meteorites. In this report, we studied their petrography and mineralogy and made in situ uranogenic Pb/Pb dating of Zr-rich minerals. Petrography and mineralogy of these two lunar meteorites are consistent with previous investigations. The zirconolite Pb/Pb age of MIL 05035 is 3851±8 Ma (2σ), in excellent agreement with previous reports. This age suggests that MIL 05035 could be paired with Asuka 881757, a low-Ti mare basalt meteorite. The magmatic event related to MIL 05035 was probably due to the late heavy impact bom- bardment on the moon around 3.9 Ga. One baddeleyite grain in LAP 02224 shows a large variation of Pb/Pb age, from 3109±29 to 3547±21 Ma (2σ), much older than the whole-rock age of the same meteorite (~3.02±0.03 Ga). The other baddeleyite grain in LAP 02224 has an age of 3005±17 Ma (2σ). The result indicates that the minimum crystallization age of LAP 02224 is ~3.55 Ga and the younger ages could reflect late thermal disturbance on U-Pb system.

绵羊体况与PMSG对同期发情效果的影响

为了研究不同体况和不同剂量孕马血清促性腺激素(PMSG)对绵羊同期发情效果的影响,试验将体况良好的60只母羊随机分为A、B两组,体况差的120只母羊随机分为C、D两组,A、C组注射400 IU PMSG,B、D组注射330 IU PMSG。结果表明:肌肉注射400 IU PMSG的母羊发情率优于肌肉注射330 IU PMSG(P<0.05),且体况良好的母羊发情率优于体况差的母羊(P<0.05)。说明母羊体况严重影响同期发情的效果,合理应用生殖激素可以提高绵羊的同期发情率。

日粮粗蛋白水平和预混料对配种前期母驴体重、血液生化指标及激素水平影响的研究

旨在研究不同蛋白水平和预混料组成日粮对配种前期母驴体重、血液生化指标及雌激素相关指标的影响。选择健康、平均体重(229.18±19.11)kg、配种前3个月的经产疆岳驴母驴60头,随机均分为日粮Ⅰ组和日粮Ⅱ组。日粮Ⅰ组饲喂低粗蛋白不含预混料的日粮,日粮Ⅱ组饲喂高蛋白+预混料的日粮,预试期10 d,正试期90 d。结果:在体重方面,日粮Ⅱ组母驴第60天重、第90天重以及总增重和日增重显著高于日粮Ⅰ组(P<0.05),分别提高了4.30%、6.05%、128.53%和131.58%;在氮、糖、脂代谢方面,日粮Ⅱ组母驴血浆中总蛋白、白蛋白、葡萄糖、甘油三酯、总胆固醇以及低密度脂蛋白胆固醇的含量显著高于日粮Ⅰ组(P<0.05),分别提高了35.01%、39.80%、2.36%、13.79%、25.00%和39.13%;在繁殖激素方面,日粮Ⅱ组母驴血浆中促卵泡素和促性腺激素释放激素的水平显著高于日粮Ⅰ组(P<0.05),分别提高了65.50%和37.13%。因此,饲喂本研究设计的高蛋白+预混料日粮能够显著提高配种前期母驴的体重,同时增加其血浆中蛋白浓度,促进促卵泡素和促性腺激素释放激素的分泌。

孕马血清促性腺激素对小鼠肝脏组织CYP17A1和PXR表达的影响

旨在了解孕马血清促性腺激素(pregnant mare serum gonadotropin,PMSG)对小鼠肝脏细胞色素P45017A1(cytochrome P45017A1,CYP17A1)和孕烷X受体(pregnane X receptor,PXR)表达的影响。将48只雌性昆明系小鼠随机分为PMSG处理组和对照组,PMSG组小鼠腹腔注射PMSG 10 IU,注射后12、24、48 h处死12只小鼠,采血分离血清并采集肝脏组织样本;对照组腹腔注射等体积生理盐水,在注射后12、24、48 h时采集4只小鼠的血液和肝脏组织样本。酶联免疫吸附试验(ELISA)检测血清PMSG含量,苏木精-伊红染色(HE)观察PMSG对肝脏组织的影响,RT-qPCR和Western blot检测肝脏组织CYP17A1和PXR表达水平,免疫组织化学染色检测CYP17A1和PXR在肝脏组织中的定位。结果显示,PMSG注射后12 h小鼠血清中PMSG含量显著升高(P<0.05),24 h和48 h时逐渐降低,但仍显著高于NC组(P<0.05);PMSG注射后12、24、48 h时,肝脏细胞水泡变性明显;注射PMSG后肝脏组织中CYP17A1和PXR基因和蛋白表达均显著下调(P<0.05);CYP17A1和PXR主要定位于肝索肝实质细胞质中。研究结果证明,PMSG能够显著抑制肝脏CYP17A1和PXR表达。

控制性超促排卵大鼠模型的建立

目的选择理想的符合临床实际的控制性超促排卵(COH)大鼠模型,为后续的实验提供良好的实验基础。方法将75只大鼠随机平均分为正常组(N),模型10组(M10),模型20组(M20),模型40组(M40)和模型100组(M100),每组各15只。各模型组分别于动情期后一日的下午5:00给予腹腔注射孕马血清促性腺激素(PMSG)10单位、20单位、40单位和100单位,并于48 h后,再分别给予腹腔注射人绒毛膜促性腺激素(HCG)10单位、20单位、40单位和100单位,注射完后立即合笼,以次日行阴道涂片见大量精子记为怀孕1 d。在第8天,行剖宫探查,记录大鼠的妊娠情况和着床的胚泡数。另取25只大鼠,按上述方法随机分对照组和不同剂量干预组,每组5只,各组大鼠在妊娠第5天取材,处理后观察子宫内膜胞饮突和LIF蛋白的表达。结果各组大鼠交配率无统计学差异(P>0.05)。与N组相比,M10组大鼠怀孕率无明显降低(P>0.05),M20、M40和M100组大鼠怀孕率明显降低(P<0.01);和N组相比,M10组大鼠胚泡着床位数无明显增加(P>0.05),M20、M40和M100组大鼠胚泡着床数明显增高(P<0.05)。和N组相比,M10大鼠子宫内膜LIF蛋白表达量无明显差异(P>0.05);M20、M40和M100组大鼠子宫内膜LIF蛋白表达量明显降低(P<0.05);M40和M100组大鼠子宫内膜LIF蛋白表达量比M20组更少,但差异无统计学意义(P>0.05)。N组大鼠在妊娠第5天子宫内膜腺上皮细胞表面胞饮突丰富;M20大鼠子宫内膜腺上皮细胞表面几乎未见明显胞饮突表达。结论M20组大鼠妊娠率较正常组明显降低,且平均胚泡数较高,胞饮突提前出现,LIF表达较正常组降低,是较为符合临床实际的理想模型。

妊娠后期饲粮中添加N-氨甲酰谷氨酸对伊犁母马血浆参数及新生马驹体重、体尺指标的影响

本试验旨在研究妊娠后期饲粮中添加N-氨甲酰谷氨酸(NCG)对伊犁母马血液参数及新生马驹体重、体尺指标的影响。按照体重、年龄、预产期相近的原则选择30匹妊娠后期[(280±15)d]伊犁母马,随机分为2组,每组15匹,其中对照组饲喂基础饲粮,NCG组在基础饲粮中添加0.17%的NCG。饲喂期共计52 d,其中预试期7 d,正试期45 d,分别于试验的第0、21、45天采集母马血液样品,分离血浆用于血浆参数的测定。结果显示:与对照组相比,NCG组新生马驹的初生重、体高显著提高(P<0.05),胸围、管围极显著提高(P<0.01)。NCG组母马分娩后的妊娠率与对照组相比提高了19.99%(P>0.05)。与对照组相比,在试验第21天,NCG组母马血浆中总一氧化氮合成酶(TNOS)、内皮型一氧化氮合成酶(eNOS)、诱导型一氧化氮合成酶(iNOS)活性差异不显著(P>0.05),一氧化氮(NO)含量极显著提高(P<0.01);在试验第45天,NCG组母马血浆中TNOS、iNOS、eNOS活性极显著降低(P<0.01)。与对照组相比,在试验第0、21天,NCG组母马血浆中尿素氮、氨含量和谷胱甘肽过氧化物酶活性差异不显著(P>0.05);在试验第0天,NCG组母马血浆中超氧化物歧化酶活性和丙二醛含量极显著升高(P<0.01);在试验第45天,NCG组母马血浆中尿素氮和丙二醛含量极显著降低(P<0.01)。试验第0、21、45天,NCG组母马血浆中生长激素、胰岛素生长因子-1含量与对照组相比均差异不显著(P>0.05)。综上可知,在伊犁母马妊娠后期饲粮中添加NCG可提高新生马驹的初生重、体尺指标及改善母马分娩后的妊娠率,提高妊娠后期母马血浆中TNOS、iNOS、eNOS活性和NO含量。NCG可以作为营养调控剂,用于繁殖母马妊娠后期或分娩后改善母马的繁殖力。

不同FSH处理方法对多浪羊FSH、LH、E2和P4激素水平及发情的影响

[目的]为了研究FSH不同注射方法对多浪羊繁殖性能的影响。[方法]试验将172只多浪羊母羊随机分为A、B、C 3个试验组和1个对照组(D)。在相同饲养条件下,于阴道放置海绵栓,埋栓后第9天先采集血液,再将150 IU FSH按照不同注射方法对A、B、C组进行处理,第11天采集血液后,撤栓注射相同剂量的孕马血清促性腺激素(PMSG)和氯前列烯醇(PG)进行同期发情处理,在发情0 h和发情12 h采集血液,采用酶联免疫吸附测定法(ELISA)测定E2、FSH、LH和P4的浓度。[结果]FSH的浓度在FSH处理前、撤栓前、发情0 h和发情12 h时,各试验组之间血清中差异不显著(P>0.05);E2的浓度在撤栓前B、C组显著高于A、D组(P<0.05),在发情0 h时,B、C组极显著高于A组(P<0.01)、D组(P<0.01),D组极显著高于A组(P<0.01)。LH的浓度在撤栓前D组显著高于A、B、C组(P<0.05),在发情0 h,B组显著高于A、C、D组(P<0.05);P4的浓度在FSH处理前、撤栓前、发情0 h和发情12 h时,各试验组之间差异不显著(P>0.05)。[结论]外源生殖激素FSH的注射会对血液中E2和LH的浓度造成影响,且发情0 h时血液中E2的浓度与发情率呈正相关。单次FSH注射剂量150 IU可能会抑制LH和E2的分泌。75 IU+75 IU平均注射法对发情率有较好的作用;100 IU+50 IU递减注射法,在发情0 h可以明显提高血液中E2和LH的浓度。

利用重力多尺度分析方法反演月球雨海地区三维月壳与上月幔结构

雨海盆地是月球正面最大的撞击盆地之一,确定其月壳与上月幔精细三维结构,对研究月球"质量瘤"形成机制等科学问题具有重要意义.常用于反演月壳与上月幔结构的月震波方法受限于月震台站稀少,给出三维结果的空间分辨率不足.随着重力测量技术的发展,月球重力数据横向分辨率逐步提高,但存在垂向分辨率不足问题.鉴于此,本文采用重力多尺度分析方法来进行改善,以获取研究区域月壳与上月幔精细三维密度结构与壳幔界面深度.研究结果表明:雨海盆地内部存在一个顶部小、底部大的高密度物质体,其底部中心位置约为经度16°W,纬度42°N;雨海盆地壳幔界面存在明显上隆,且盆地内部上隆趋势不平滑,发现有类环状异变现象;雨海"质量瘤"可能是由内部高密度物质和壳幔上隆共同推动形成的;重力多尺度分析方法可以有效提升重力数据的垂向识别能力,获取更为精细的三维月壳与上月幔结构.