Microbial ectoenzymes play essential roles in the transformation and mineralization of organic matter in the ocean.However,conventional methods for measuring microbial ectoenzyme activities(MEAs)in the deep ocean unde...Microbial ectoenzymes play essential roles in the transformation and mineralization of organic matter in the ocean.However,conventional methods for measuring microbial ectoenzyme activities(MEAs)in the deep ocean under atmospheric conditions can misrepresent the metabolic activities of indigenous microbial communities.To overcome this limitation,we measured the activities of three microbial ectoenzymes(aminopeptidase,α-glucosidase,andβ-glucosidase)under both atmospheric and in situ pressure conditions in seawater collected using pressure-retaining and non-pressure-retaining samplers in the Challenger Deep,Mariana Trench.In the case of aminopeptidase andα-glucosidase,the highest isobaric MEAs were observed in the surface layer(50 m),followed by those at abyssopelagic depths(4,000–6,000 m)for aminopeptidase,hadal realm(10,903 m)forα-glucosidase,whileβ-glucosidase activity exhibited the highest value at 10,903 m.Furthermore,the isobaric MEAs in hadal waters were commonly found to be higher than the decompressed counterparts,highlighting the importance of pressure-retaining sampling and isobaric enzymatic assays.The half-saturation constant(K_(m))showed a general decreasing trend with depth,suggesting that the deep ocean microbes might have adapted to the high-pressure and oligotrophic environment by increasing their ectoenzyme's affinity to substrate.Furthermore,particle-free MEAs contribute more to the total MEAs in the deep ocean than particle-associated MEAs,suggesting the significance of cell-associated and dissolved ectoenzymes in ocean ecosystems.This study provides a foundation for future investigations of MEAs in the ocean and has important implications for understanding the dynamics of microbially mediated biogeochemical cycling in marine ecosystems.展开更多
Iddingsitization is an aqueous alteration that is known to take place in meteorites and continental basalts providing a potential habitat for microbial life.However,little is known about the exact mode by which this r...Iddingsitization is an aqueous alteration that is known to take place in meteorites and continental basalts providing a potential habitat for microbial life.However,little is known about the exact mode by which this reaction occurs in the hadal seafloor and its implication for the deep subsurface biosphere.A comprehensive investigation of hadal basalts from the southern Mariana Trench(SMT)conducted with microscopic examinations shows that iddingsite occurs as augite-hosted veins,rims,and mineral grains formed completely in place of augite within the SMT basalts.Carbon geochemistry indicates that organic matter with homogenousδ^(13)C values between−27.8‰and−27.2‰might be biogenically accumulated in the SMT basalts.Furthermore,the close spatial relationships between carbonaceous matter(CM)and goethite in iddingsite point to microbial attachment to iddingsite minerals.Thus,iddingsitization might have fueled H_(2)-utilizing microorganisms inhabiting the hadal oceanic crust,thereby leading to the formation of CM,as implied by oxygen isotopic compositions revealing low alteration temperatures(32-83℃)favorable for microbial growth.In all,microbial biosignatures associated with iddingsite in the SMT basalts are highlighted,and these results could pave the way for deciphering the deep subsurface biosphere at hadal zones.展开更多
为解决运动想象脑电(electroencephalogram, EEG)信号多分类传输速率慢、准确率低的问题,本研究利用“一对多”滤波组共空间模式(one vs rest filter bank common spatial pattern, OVR-FBCSP)和稀疏嵌入(sparse embeddings, SE)提出了...为解决运动想象脑电(electroencephalogram, EEG)信号多分类传输速率慢、准确率低的问题,本研究利用“一对多”滤波组共空间模式(one vs rest filter bank common spatial pattern, OVR-FBCSP)和稀疏嵌入(sparse embeddings, SE)提出了一种基于SE的多分类EEG信号分类方法。为降低多类任务特征提取的复杂度,提高分类效率,本方法首先采用OVR-FBCSP进行EEG信号特征提取;然后对其相应的标签矩阵进行低维嵌入,构建稀疏嵌入模型,分别计算训练和测试数据的嵌入矩阵;最后在嵌入空间中对训练和测试数据执行k最近邻(k-nearest neighbor, kNN)分类。本研究在BCI Competition IV-2a公开数据集进行了实验测试,并与其他分类方法进行了对比。实验结果表明,本研究方法拥有较高的分类准确率和较短的分析时间。展开更多
基金supported by the National Natural Science Foundation of China(Grant No.92251303)the Shanghai Municipal Education Commission(Grant No.2023ZKZD53)the Marine Biomedical Science and Technology Innovation Platform。
文摘Microbial ectoenzymes play essential roles in the transformation and mineralization of organic matter in the ocean.However,conventional methods for measuring microbial ectoenzyme activities(MEAs)in the deep ocean under atmospheric conditions can misrepresent the metabolic activities of indigenous microbial communities.To overcome this limitation,we measured the activities of three microbial ectoenzymes(aminopeptidase,α-glucosidase,andβ-glucosidase)under both atmospheric and in situ pressure conditions in seawater collected using pressure-retaining and non-pressure-retaining samplers in the Challenger Deep,Mariana Trench.In the case of aminopeptidase andα-glucosidase,the highest isobaric MEAs were observed in the surface layer(50 m),followed by those at abyssopelagic depths(4,000–6,000 m)for aminopeptidase,hadal realm(10,903 m)forα-glucosidase,whileβ-glucosidase activity exhibited the highest value at 10,903 m.Furthermore,the isobaric MEAs in hadal waters were commonly found to be higher than the decompressed counterparts,highlighting the importance of pressure-retaining sampling and isobaric enzymatic assays.The half-saturation constant(K_(m))showed a general decreasing trend with depth,suggesting that the deep ocean microbes might have adapted to the high-pressure and oligotrophic environment by increasing their ectoenzyme's affinity to substrate.Furthermore,particle-free MEAs contribute more to the total MEAs in the deep ocean than particle-associated MEAs,suggesting the significance of cell-associated and dissolved ectoenzymes in ocean ecosystems.This study provides a foundation for future investigations of MEAs in the ocean and has important implications for understanding the dynamics of microbially mediated biogeochemical cycling in marine ecosystems.
基金funded by National Natural Science Foundation of China(Grant Nos.42206045 and 42202197)Natural Science Foundation of Hebei Province(Grant No.D2020205007)+1 种基金Science and Technology Project of Hebei Education Department(Grant No.QN2023167)Science Foundation of Hebei Normal University(Grant Nos.L2020B24,L2021B21)。
文摘Iddingsitization is an aqueous alteration that is known to take place in meteorites and continental basalts providing a potential habitat for microbial life.However,little is known about the exact mode by which this reaction occurs in the hadal seafloor and its implication for the deep subsurface biosphere.A comprehensive investigation of hadal basalts from the southern Mariana Trench(SMT)conducted with microscopic examinations shows that iddingsite occurs as augite-hosted veins,rims,and mineral grains formed completely in place of augite within the SMT basalts.Carbon geochemistry indicates that organic matter with homogenousδ^(13)C values between−27.8‰and−27.2‰might be biogenically accumulated in the SMT basalts.Furthermore,the close spatial relationships between carbonaceous matter(CM)and goethite in iddingsite point to microbial attachment to iddingsite minerals.Thus,iddingsitization might have fueled H_(2)-utilizing microorganisms inhabiting the hadal oceanic crust,thereby leading to the formation of CM,as implied by oxygen isotopic compositions revealing low alteration temperatures(32-83℃)favorable for microbial growth.In all,microbial biosignatures associated with iddingsite in the SMT basalts are highlighted,and these results could pave the way for deciphering the deep subsurface biosphere at hadal zones.
