Improving grain quality is an important goal in breeding new elite rice varieties,requiring effective tools for the identification of target genotypes.Molecular marker-aided selection(MAS),combined with conventional b...Improving grain quality is an important goal in breeding new elite rice varieties,requiring effective tools for the identification of target genotypes.Molecular marker-aided selection(MAS),combined with conventional breeding approaches,enables us to pre-cisely identify the individual genotypes that are associated with different grain quality features,which can dramatically improve the breeding efficiency.However,to date,the number of molecular markers used in MAS for grain quality improvement is still somewhat limited.In this study,based on our previous study that rice grain quality is strongly associated with starch synthesis in the endosperm,we developed 51 gene-tagged molecular markers according to sequence variations in 18 starch synthesis-related genes from 16 typical rice cultivars.These markers can discriminate the different alleles among rice germplasms.These novel markers will provide effective tools in improving grain quality via the breeding new elite rice varieties.展开更多
There are nearly 1 000 rice landrace varieties in the Taihu basin, China. To assess the genetic diversity of the rice, 24 intragenic molecular markers（representing 17 starch synthesis-related genes） were investigate...There are nearly 1 000 rice landrace varieties in the Taihu basin, China. To assess the genetic diversity of the rice, 24 intragenic molecular markers（representing 17 starch synthesis-related genes） were investigated in 115 Taihu basin rice landraces and 87 improved cultivars simultaneously. The results show that the average genetic diversity and polymorphism information content values of the landraces were higher than those of improved cultivars. In total, 41 and 39 allele combinations（of the 17 genes） were derived from the landraces and improved cultivars, respectively; only two identical allele combinations were found bet ween the two rice variety sources. Cluster analysis, based on the molecular markers, revealed that the rice varieties could be subdivided into five groups and, within these, the japonica improved rice and japonica landrace rice varieties were in two separate groups. According to the quality reference criteria to classify the rice into grades, some of the landraces were found to perform we ll, in terms of starch quality. For example, according to NY /T595-2002 criteria from the Ministry of Agriculture of China, 25 and 33 landraces reached grade 1, in terms of their apparent amylose content and gel consistency. Th e varieties that had outstanding quality could be used as breeding materials for rice quality breeding programs in the future. Our study is useful for future applications, such as genetic diversity studies, the protection of rice variety and improvment of rice quality in breeding programs.展开更多
基于2-苯基萘型结构,以2-甲基吲哚和四氯苯醌、1-二甲基胺-2-丙炔和碘甲基硼酸频哪醇酯作为起始底物,经5步反应合成了一种吲哚苯醌类分子探针的前体,其结构经1 H NMR,^(13)C NMR和HR-MS(ESI)表征。对该前体进行放射性标记,再经高效液相...基于2-苯基萘型结构,以2-甲基吲哚和四氯苯醌、1-二甲基胺-2-丙炔和碘甲基硼酸频哪醇酯作为起始底物,经5步反应合成了一种吲哚苯醌类分子探针的前体,其结构经1 H NMR,^(13)C NMR和HR-MS(ESI)表征。对该前体进行放射性标记,再经高效液相色谱分析纯化,最后将探针注入小鼠体内进行正电子发射型计算机断层显像(PET)。结果表明:探针分子进入小鼠体内后,在膀胱、胆和肠道聚集,随后经肠道和膀胱代谢排出体外。展开更多
In this paper,with 511 rice landraces in the Taihu Lake area as test materials,we select 19 starch synthesis-related intragenic molecular markers to detect the genetic quality of starch,and compare them with 86 bred v...In this paper,with 511 rice landraces in the Taihu Lake area as test materials,we select 19 starch synthesis-related intragenic molecular markers to detect the genetic quality of starch,and compare them with 86 bred varieties. The results show that the average polymorphic information content( PIC) of japonica landraces is 0. 1726,slightly higher than the average PIC( 0. 1101) of the bred japonica rice varieties.Based on Nei's genetic distance between materials,UPGMA method is used for clustering,and all study materials are divided into 6 groups.Group I mainly includes indica rice,the bred japonica rice varieties are mainly concentrated in the first half of Group II and Group III,and the japonica landraces are mainly concentrated in the second half of Group III,and Group Ⅳ,Ⅴ,Ⅵ. Both of them are in different regions,and there has been genetic differentiation. According to the national standard of high quality rice,it is found that many rice landraces in the Taihu Lake area have good quality and traits,and these varieties can be used for future high quality breeding.展开更多
基金supported by the National High Technology Research and Development Program of China(2006AA10Z118,2006AA10A102,2005CB120804)National Natural Science Foundation of China(30530470)+1 种基金Fok Ying Tung Young Teachers'Fund(10103)National Postdoctoral Fellowship(20060390966)
文摘Improving grain quality is an important goal in breeding new elite rice varieties,requiring effective tools for the identification of target genotypes.Molecular marker-aided selection(MAS),combined with conventional breeding approaches,enables us to pre-cisely identify the individual genotypes that are associated with different grain quality features,which can dramatically improve the breeding efficiency.However,to date,the number of molecular markers used in MAS for grain quality improvement is still somewhat limited.In this study,based on our previous study that rice grain quality is strongly associated with starch synthesis in the endosperm,we developed 51 gene-tagged molecular markers according to sequence variations in 18 starch synthesis-related genes from 16 typical rice cultivars.These markers can discriminate the different alleles among rice germplasms.These novel markers will provide effective tools in improving grain quality via the breeding new elite rice varieties.
基金financially supported by the National Natural Science Foundation of China(30800603)the Science and Technology Plan Projects of Taicang City,China(TC214YY3)the Building Program of the Science and Technology Innovation Team of Chien-Shiung Institute of Technology,China(2013CX02)
文摘There are nearly 1 000 rice landrace varieties in the Taihu basin, China. To assess the genetic diversity of the rice, 24 intragenic molecular markers（representing 17 starch synthesis-related genes） were investigated in 115 Taihu basin rice landraces and 87 improved cultivars simultaneously. The results show that the average genetic diversity and polymorphism information content values of the landraces were higher than those of improved cultivars. In total, 41 and 39 allele combinations（of the 17 genes） were derived from the landraces and improved cultivars, respectively; only two identical allele combinations were found bet ween the two rice variety sources. Cluster analysis, based on the molecular markers, revealed that the rice varieties could be subdivided into five groups and, within these, the japonica improved rice and japonica landrace rice varieties were in two separate groups. According to the quality reference criteria to classify the rice into grades, some of the landraces were found to perform we ll, in terms of starch quality. For example, according to NY /T595-2002 criteria from the Ministry of Agriculture of China, 25 and 33 landraces reached grade 1, in terms of their apparent amylose content and gel consistency. Th e varieties that had outstanding quality could be used as breeding materials for rice quality breeding programs in the future. Our study is useful for future applications, such as genetic diversity studies, the protection of rice variety and improvment of rice quality in breeding programs.
文摘基于2-苯基萘型结构,以2-甲基吲哚和四氯苯醌、1-二甲基胺-2-丙炔和碘甲基硼酸频哪醇酯作为起始底物,经5步反应合成了一种吲哚苯醌类分子探针的前体,其结构经1 H NMR,^(13)C NMR和HR-MS(ESI)表征。对该前体进行放射性标记,再经高效液相色谱分析纯化,最后将探针注入小鼠体内进行正电子发射型计算机断层显像(PET)。结果表明:探针分子进入小鼠体内后,在膀胱、胆和肠道聚集,随后经肠道和膀胱代谢排出体外。
基金Supported by Taicang Science and Technology Project(TC2014YY03)Science and Technology Innovation Team Building Project of Suzhou Chien-shiung Institute of Technology(2013CX02)
文摘In this paper,with 511 rice landraces in the Taihu Lake area as test materials,we select 19 starch synthesis-related intragenic molecular markers to detect the genetic quality of starch,and compare them with 86 bred varieties. The results show that the average polymorphic information content( PIC) of japonica landraces is 0. 1726,slightly higher than the average PIC( 0. 1101) of the bred japonica rice varieties.Based on Nei's genetic distance between materials,UPGMA method is used for clustering,and all study materials are divided into 6 groups.Group I mainly includes indica rice,the bred japonica rice varieties are mainly concentrated in the first half of Group II and Group III,and the japonica landraces are mainly concentrated in the second half of Group III,and Group Ⅳ,Ⅴ,Ⅵ. Both of them are in different regions,and there has been genetic differentiation. According to the national standard of high quality rice,it is found that many rice landraces in the Taihu Lake area have good quality and traits,and these varieties can be used for future high quality breeding.
