Orthogonal combination design was adopted in examining the Spirulina platensis (S.platensis) yield and the influence of four factors (Se content, Se-adding method, S content and NaHCO 3 content) on algae growth. The r...Orthogonal combination design was adopted in examining the Spirulina platensis (S.platensis) yield and the influence of four factors (Se content, Se-adding method, S content and NaHCO 3 content) on algae growth. The results showed that Se content, Se-adding method and NaHCO 3 content were key factors in cultivation conditions of Se-enriched S.platensis with the optimal combination being Se at 300mg/L, Se-adding amount equally divided into three times and NaHCO 3 at 16.8 g/L. Algae yield had a remarkable correlation with OD 560 and floating rate by linear regression analysis. There was a corresponding relationship between effects of the four factors on algae yield and on OD 560 , floating rate too. In conclusion, OD 560 and floating rate could be served as yield-forming factors.展开更多
本实验利用云南永胜县程海湖的碱水配制培养基,在当地的气候条件下驯化培养钝顶螺旋藻(Spirulina platensis),筛选得CH-1,CH-2和CH-3三种培养基配方。室外连续培养共240天,其中采用CH-2培养基室外大量培养90天,培养面积75平方米,连续测...本实验利用云南永胜县程海湖的碱水配制培养基,在当地的气候条件下驯化培养钝顶螺旋藻(Spirulina platensis),筛选得CH-1,CH-2和CH-3三种培养基配方。室外连续培养共240天,其中采用CH-2培养基室外大量培养90天,培养面积75平方米,连续测定20天的干藻粉产量为12.5克/米~2·天,纯度为95%,干藻粉的蛋白质含量55—63%。除色氨酸未测定外,含有另外的17种氨基酸。从程海碱水驯化培养的钝顶螺旋藻群体中,筛选获得一个螺旋藻新品系——Spirulinaplatensis str. CH-1。与原品系比较,其特点是藻丝和螺距都较长,螺旋更宽,具有上浮性较好的优势,适合在用程海碱水配制的培养基中生长。实验结果表明,利用程海丰富的湖水资源和适宜的气候条件,发展螺旋藻生产是可行的。展开更多
文摘Orthogonal combination design was adopted in examining the Spirulina platensis (S.platensis) yield and the influence of four factors (Se content, Se-adding method, S content and NaHCO 3 content) on algae growth. The results showed that Se content, Se-adding method and NaHCO 3 content were key factors in cultivation conditions of Se-enriched S.platensis with the optimal combination being Se at 300mg/L, Se-adding amount equally divided into three times and NaHCO 3 at 16.8 g/L. Algae yield had a remarkable correlation with OD 560 and floating rate by linear regression analysis. There was a corresponding relationship between effects of the four factors on algae yield and on OD 560 , floating rate too. In conclusion, OD 560 and floating rate could be served as yield-forming factors.
文摘本实验利用云南永胜县程海湖的碱水配制培养基,在当地的气候条件下驯化培养钝顶螺旋藻(Spirulina platensis),筛选得CH-1,CH-2和CH-3三种培养基配方。室外连续培养共240天,其中采用CH-2培养基室外大量培养90天,培养面积75平方米,连续测定20天的干藻粉产量为12.5克/米~2·天,纯度为95%,干藻粉的蛋白质含量55—63%。除色氨酸未测定外,含有另外的17种氨基酸。从程海碱水驯化培养的钝顶螺旋藻群体中,筛选获得一个螺旋藻新品系——Spirulinaplatensis str. CH-1。与原品系比较,其特点是藻丝和螺距都较长,螺旋更宽,具有上浮性较好的优势,适合在用程海碱水配制的培养基中生长。实验结果表明,利用程海丰富的湖水资源和适宜的气候条件,发展螺旋藻生产是可行的。
