Mastocytoma usually presents as a solitary lesion whereas mastocytosis is a rare skin condition which typically manifests with or without accompanied systemic symptoms. Although a few cases of solitary mastocytoma hav...Mastocytoma usually presents as a solitary lesion whereas mastocytosis is a rare skin condition which typically manifests with or without accompanied systemic symptoms. Although a few cases of solitary mastocytoma have been documented on mucosa (vulva), none has been reported on the oral mucosa in the English literature. The present case is an example of an exceptional rare presentation of solitary mastocytoma of the oral mucosa.展开更多
The cytotoxicity and apoptotic effect of phytoceramide IV （N-palmitoyl-phytospingosine） were investigated by preparaing the ceramide containing liposomes using cholesteryl hemisuccinate （CHEMS） and methoxypolyethy...The cytotoxicity and apoptotic effect of phytoceramide IV （N-palmitoyl-phytospingosine） were investigated by preparaing the ceramide containing liposomes using cholesteryl hemisuccinate （CHEMS） and methoxypolyethylene glycol （2000） cholesteryl succinate （PEGCHS）. The ceramide-containing liposomes prepared using ultrasonication contained CHEMS and the phytoceramide IV at a molar ratio of 1：1. Gel chromatography and high performance liquid chromatography equipped with an evaporative light-scattering detection （HPLC-ELSD） were used to analyze the liposomes. The results show that the ceramide entrapment efficiency is over 90% and the molar ratio of phytoceramide IV to CHEMS is 1：1.4. The ratio of ceramide to CHEMS as well as the ultrasonication duration affects the liposome properties. The phytoceramide Ⅳ encapsulated in the liposomes reduces the cellular activity of the murine mastocytoma cell line P815 in a dose-dependent manner and the reduction of cellular activity is due to cell apoptosis. 4. 6-diamidino- 2-phenylindole dihydrochloride （DAPI） staining further supports this result. The encapsulation of highly hydrophobic ceramides into liposomal formulations could become a potential candidate for enhanced apoptosis.展开更多
Mastocytosis is a rare skin condition which typically .manifests with or without accompanied systemic symptoms. Localized tissue mastocytosis is known as mastocytoma. A Mastocytoma localized to the vulva is an extreme...Mastocytosis is a rare skin condition which typically .manifests with or without accompanied systemic symptoms. Localized tissue mastocytosis is known as mastocytoma. A Mastocytoma localized to the vulva is an extremely rare condition only a few known cases of this condition have been reported so far. Due to the scarcity of this ailment no consensus about the diagnosis, management and treatment has been reached. In this case, we reported a 23-year-old female patient with a mastocytoma that had been growing for 18 years.展开更多
文摘Mastocytoma usually presents as a solitary lesion whereas mastocytosis is a rare skin condition which typically manifests with or without accompanied systemic symptoms. Although a few cases of solitary mastocytoma have been documented on mucosa (vulva), none has been reported on the oral mucosa in the English literature. The present case is an example of an exceptional rare presentation of solitary mastocytoma of the oral mucosa.
基金the National Natural Science Foundation of China (Nos. 30170017 and 50436040)the National Natural Science Foundation for Outstanding Young Investigator (No. 30225001)
文摘The cytotoxicity and apoptotic effect of phytoceramide IV （N-palmitoyl-phytospingosine） were investigated by preparaing the ceramide containing liposomes using cholesteryl hemisuccinate （CHEMS） and methoxypolyethylene glycol （2000） cholesteryl succinate （PEGCHS）. The ceramide-containing liposomes prepared using ultrasonication contained CHEMS and the phytoceramide IV at a molar ratio of 1：1. Gel chromatography and high performance liquid chromatography equipped with an evaporative light-scattering detection （HPLC-ELSD） were used to analyze the liposomes. The results show that the ceramide entrapment efficiency is over 90% and the molar ratio of phytoceramide IV to CHEMS is 1：1.4. The ratio of ceramide to CHEMS as well as the ultrasonication duration affects the liposome properties. The phytoceramide Ⅳ encapsulated in the liposomes reduces the cellular activity of the murine mastocytoma cell line P815 in a dose-dependent manner and the reduction of cellular activity is due to cell apoptosis. 4. 6-diamidino- 2-phenylindole dihydrochloride （DAPI） staining further supports this result. The encapsulation of highly hydrophobic ceramides into liposomal formulations could become a potential candidate for enhanced apoptosis.
文摘Mastocytosis is a rare skin condition which typically .manifests with or without accompanied systemic symptoms. Localized tissue mastocytosis is known as mastocytoma. A Mastocytoma localized to the vulva is an extremely rare condition only a few known cases of this condition have been reported so far. Due to the scarcity of this ailment no consensus about the diagnosis, management and treatment has been reached. In this case, we reported a 23-year-old female patient with a mastocytoma that had been growing for 18 years.
