This study investigated the influence of activities of glyceraldehyde-3-phosphate dehydrogenase （GAPDH） and lactate dehydrogenase-B （LDH-B） on the colour stability of mutton. From 60 sheep （Bayannur mutton sheep...This study investigated the influence of activities of glyceraldehyde-3-phosphate dehydrogenase （GAPDH） and lactate dehydrogenase-B （LDH-B） on the colour stability of mutton. From 60 sheep （Bayannur mutton sheep）, 15 Iongissimus dorsi （LD） muscles were selected on the basis of colour stability （R630/580 and a＊ value） during the storage and classified into three groups （5 for each group） as high colour stability （HCS）, intermediate colour stability （ICS） and low colour stability （LCS）. The activities of GAPDH and LDH-B, muscle colour attributes, nicotinamide adenine dinucleuotide （NADH） concen- tration and lactate concentration were measured. The samples in HCS had higher activities of GAPDH and LDH-B than the samples in the LCS, and the samples in the HCS group also possessed higher NADH and lower lactate concentration. The higher activity of dehydrogenase enzyme may result in higher NADH concentrations and colour stability in muscle tissue. The results suggest that the activity of GAPDH and LDH-B may also play a role in maintaining colour stability.展开更多
以白色杜洛克×二花脸资源家系冬、夏两季的两批F2屠宰个体为材料,分析比较两个季节组背最长肌、半膜肌pH值和肉色间差异及pH值对肉色影响的差异。结果显示:背最长肌和半膜肌pH值和肉色均受季节的显著影响。冬季组背最长肌45 m in p...以白色杜洛克×二花脸资源家系冬、夏两季的两批F2屠宰个体为材料,分析比较两个季节组背最长肌、半膜肌pH值和肉色间差异及pH值对肉色影响的差异。结果显示:背最长肌和半膜肌pH值和肉色均受季节的显著影响。冬季组背最长肌45 m in pH值显著高于夏季组,而2种肌肉24 h pH值在两组间无差异;夏季组2种肌肉的L*值均显著高于冬季组,a*和b*值显著低于冬季组。2组的2种肌肉pH值与L*值显著中度相关,a*值和b*值与pH值仅在夏季组中呈中度相关。展开更多
为了揭示猪不同肉质样品内参蛋白表达的稳定性,试验测定了北京黑猪宰后24小时pH值、肉色(L^*)值以及72小时滴水损失(drip loss,DL)值,并根据测定结果挑选出PSE(pale soft and exudative)及RFN(red firm and non-exudative)2种肉样,采用W...为了揭示猪不同肉质样品内参蛋白表达的稳定性,试验测定了北京黑猪宰后24小时pH值、肉色(L^*)值以及72小时滴水损失(drip loss,DL)值,并根据测定结果挑选出PSE(pale soft and exudative)及RFN(red firm and non-exudative)2种肉样,采用Western-blot方法检测2种肉样中3种内参蛋白β-Actin、Gapdh及β-Tubulin的表达情况。结果表明:3种内参蛋白在2种肉样中均能稳定表达,但与β-Tubulin相比,β-Actin和Gapdh的表达丰度更高。说明β-Actin、Gapdh及β-Tubulin均适合作为量化猪不同肉质样品蛋白表达的内参蛋白。展开更多
The objective of this work is to show the effect of residual blood on the quality of chicken meat, work based on the hypothesis that increasing the concentration of residual blood would explain the observed quality de...The objective of this work is to show the effect of residual blood on the quality of chicken meat, work based on the hypothesis that increasing the concentration of residual blood would explain the observed quality degradation by several authors. Lots of chickens are killed with two different types of slaughter: Slaughter without stunning for a maximum flow of blood or bleeding perfect PS, shooting seals slight lead to keep almost all blood from the carcass or bleeding IS flawed. To prove the residual blood factors that are responsible for high pHu level and high values of (L* A* B*) in poultry, this study compares the pHu level and values of (L* A* B*) in poultry both perfectly and imperfectly bled. Samples from imperfectly bled carcasses show a high value average number of A* = 12.68 and B* = 16.85;and L* = 54.09 VS the average number of A* = 8.50 and B* = 14.43 and L* = 50.27. At 3℃ storage temperature, the average pHu of perfectly bled poultry was (5.7) which was significantly (P < 0.01) lower than the average pHu of imperfectly bled poultry (6.08). At 7℃ storage temperature, the average pHu of perfectly bled poultry was (6.07) which was significantly (P < 0.05) lower than the average pHu of imperfectly bled one (6.27). We found out the effect of season on the results of pHu measures, so we conducted aanalysis of pHu in summer and winter. Residual blood of the poultry is responsible for high values of (L* A* B*) and high values of pHu therefore the deterioration of the poultry Colour.展开更多
Fifty four, seven month old lambs, that had grazed perennial or annual pasture, were slaughtered and at 24 h post mortem m. longissimus lumborum samples were collected. Half of the fresh muscle section was sliced into...Fifty four, seven month old lambs, that had grazed perennial or annual pasture, were slaughtered and at 24 h post mortem m. longissimus lumborum samples were collected. Half of the fresh muscle section was sliced into three pieces and overwrapped with 15 micron polyvinyl chloride film and displayed under light (Lux = 1500) at 3℃ - 4℃. The remainder of the muscle section was vacuum packaged and aged for 4 weeks at 3℃, before slicing and display. Surface brownness and redness were measured over 3 days of simulated retail display. Aging in vacuum packs led to substantially less brownness and greater redness compared with fresh meat, over the entire three days of display. It was concluded that aging in vacuum packs could be a useful strategy for improving consumer acceptability of retail lamb in local markets, even where there is no logistical or transport need to extend the life of the meat.展开更多
基金financial support from the National Agricultural Science and Technology Innovation Program,Chinathe Special Fund for Agro-scientific Research in the Public Interest in China(201303083)the earmarked fund for China Agriculture Research System(CARS-39)
文摘This study investigated the influence of activities of glyceraldehyde-3-phosphate dehydrogenase （GAPDH） and lactate dehydrogenase-B （LDH-B） on the colour stability of mutton. From 60 sheep （Bayannur mutton sheep）, 15 Iongissimus dorsi （LD） muscles were selected on the basis of colour stability （R630/580 and a＊ value） during the storage and classified into three groups （5 for each group） as high colour stability （HCS）, intermediate colour stability （ICS） and low colour stability （LCS）. The activities of GAPDH and LDH-B, muscle colour attributes, nicotinamide adenine dinucleuotide （NADH） concen- tration and lactate concentration were measured. The samples in HCS had higher activities of GAPDH and LDH-B than the samples in the LCS, and the samples in the HCS group also possessed higher NADH and lower lactate concentration. The higher activity of dehydrogenase enzyme may result in higher NADH concentrations and colour stability in muscle tissue. The results suggest that the activity of GAPDH and LDH-B may also play a role in maintaining colour stability.
文摘以白色杜洛克×二花脸资源家系冬、夏两季的两批F2屠宰个体为材料,分析比较两个季节组背最长肌、半膜肌pH值和肉色间差异及pH值对肉色影响的差异。结果显示:背最长肌和半膜肌pH值和肉色均受季节的显著影响。冬季组背最长肌45 m in pH值显著高于夏季组,而2种肌肉24 h pH值在两组间无差异;夏季组2种肌肉的L*值均显著高于冬季组,a*和b*值显著低于冬季组。2组的2种肌肉pH值与L*值显著中度相关,a*值和b*值与pH值仅在夏季组中呈中度相关。
文摘为了揭示猪不同肉质样品内参蛋白表达的稳定性,试验测定了北京黑猪宰后24小时pH值、肉色(L^*)值以及72小时滴水损失(drip loss,DL)值,并根据测定结果挑选出PSE(pale soft and exudative)及RFN(red firm and non-exudative)2种肉样,采用Western-blot方法检测2种肉样中3种内参蛋白β-Actin、Gapdh及β-Tubulin的表达情况。结果表明:3种内参蛋白在2种肉样中均能稳定表达,但与β-Tubulin相比,β-Actin和Gapdh的表达丰度更高。说明β-Actin、Gapdh及β-Tubulin均适合作为量化猪不同肉质样品蛋白表达的内参蛋白。
文摘The objective of this work is to show the effect of residual blood on the quality of chicken meat, work based on the hypothesis that increasing the concentration of residual blood would explain the observed quality degradation by several authors. Lots of chickens are killed with two different types of slaughter: Slaughter without stunning for a maximum flow of blood or bleeding perfect PS, shooting seals slight lead to keep almost all blood from the carcass or bleeding IS flawed. To prove the residual blood factors that are responsible for high pHu level and high values of (L* A* B*) in poultry, this study compares the pHu level and values of (L* A* B*) in poultry both perfectly and imperfectly bled. Samples from imperfectly bled carcasses show a high value average number of A* = 12.68 and B* = 16.85;and L* = 54.09 VS the average number of A* = 8.50 and B* = 14.43 and L* = 50.27. At 3℃ storage temperature, the average pHu of perfectly bled poultry was (5.7) which was significantly (P < 0.01) lower than the average pHu of imperfectly bled poultry (6.08). At 7℃ storage temperature, the average pHu of perfectly bled poultry was (6.07) which was significantly (P < 0.05) lower than the average pHu of imperfectly bled one (6.27). We found out the effect of season on the results of pHu measures, so we conducted aanalysis of pHu in summer and winter. Residual blood of the poultry is responsible for high values of (L* A* B*) and high values of pHu therefore the deterioration of the poultry Colour.
文摘Fifty four, seven month old lambs, that had grazed perennial or annual pasture, were slaughtered and at 24 h post mortem m. longissimus lumborum samples were collected. Half of the fresh muscle section was sliced into three pieces and overwrapped with 15 micron polyvinyl chloride film and displayed under light (Lux = 1500) at 3℃ - 4℃. The remainder of the muscle section was vacuum packaged and aged for 4 weeks at 3℃, before slicing and display. Surface brownness and redness were measured over 3 days of simulated retail display. Aging in vacuum packs led to substantially less brownness and greater redness compared with fresh meat, over the entire three days of display. It was concluded that aging in vacuum packs could be a useful strategy for improving consumer acceptability of retail lamb in local markets, even where there is no logistical or transport need to extend the life of the meat.
