Monitoring of food borne pathogens in food is the primary tool for the implementation of food safety systems. It is necessary to monitor the prevalence of food borne pathogens for effective food safety planning and ta...Monitoring of food borne pathogens in food is the primary tool for the implementation of food safety systems. It is necessary to monitor the prevalence of food borne pathogens for effective food safety planning and targeted interventions. Staphylococcus aureus is considered as the third largest cause of food related illness in worldwide. The present study aimed at surveillance of S. aureus contamination of meat on meat supply chain stages, which is a common benchmark of meat market in Mongolia, and characterization of isolated and collected strains from other agricultural sources. The cultural and polymerase chain reaction （PCR） methods were used for isolation, identification and characterization of S. aureus. In 216 cultures of S. aureus among 634 Staphylococci isolates obtained from different sources throughout the agricultural production chain in this study, common gene for S. aureus （98.74%）, and nuc （97.47%）, mecA （44.12%）, msrA （9.66%）, gyrA （32.77%） and ermC （29.41%） genes were identified. As seen in the surveillance result, the prevalence of methicillin-resistance S. aureus （MRSA） is 44% among S. aureus isolates from agricultural production chain. Confirmed cases of food-borne infections and intoxications caused by S. aureus should be considered as one of mean criteria of food safety issues in Mongolia, and special attentions should be paid on antibiotic resistant bacteria, such as S. aureus.展开更多
文摘Monitoring of food borne pathogens in food is the primary tool for the implementation of food safety systems. It is necessary to monitor the prevalence of food borne pathogens for effective food safety planning and targeted interventions. Staphylococcus aureus is considered as the third largest cause of food related illness in worldwide. The present study aimed at surveillance of S. aureus contamination of meat on meat supply chain stages, which is a common benchmark of meat market in Mongolia, and characterization of isolated and collected strains from other agricultural sources. The cultural and polymerase chain reaction （PCR） methods were used for isolation, identification and characterization of S. aureus. In 216 cultures of S. aureus among 634 Staphylococci isolates obtained from different sources throughout the agricultural production chain in this study, common gene for S. aureus （98.74%）, and nuc （97.47%）, mecA （44.12%）, msrA （9.66%）, gyrA （32.77%） and ermC （29.41%） genes were identified. As seen in the surveillance result, the prevalence of methicillin-resistance S. aureus （MRSA） is 44% among S. aureus isolates from agricultural production chain. Confirmed cases of food-borne infections and intoxications caused by S. aureus should be considered as one of mean criteria of food safety issues in Mongolia, and special attentions should be paid on antibiotic resistant bacteria, such as S. aureus.
