[Objectives] To identify the species of Hirudo from different sources. [Methods] In accordance with morphological, anatomical and molecular taxonomic characteristics, the identification conclusion was drawn. [Results]...[Objectives] To identify the species of Hirudo from different sources. [Methods] In accordance with morphological, anatomical and molecular taxonomic characteristics, the identification conclusion was drawn. [Results] The living samples of Hirudo belong to 1 order, 2 families, 3 genera and 5 species, and the dry samples of medicinal leeches belong to 4 species. [Conclusions] The sources of medicinal leeches are complex, and there is a problem that Hirudo from different basic sources are mixed for medication.展开更多
Antifungal activity of recombinant medicinal leech destabilase-lysozyme （rec.Dest-Lys） was investi- gated by using fungi： Botrytis cinerea, and Verticillium lateriticum, including yeasts Candida guillermoudii and S...Antifungal activity of recombinant medicinal leech destabilase-lysozyme （rec.Dest-Lys） was investi- gated by using fungi： Botrytis cinerea, and Verticillium lateriticum, including yeasts Candida guillermoudii and Shizosaccharomyces pombe. Its antibacterial activity was investigated on gram-negative bacteria Pseudomonas fluorescens. These activities were assessed by radial agar diffusion assay, and scanning and transmission electron microscopy. Therefore, destabilase-lysozyme not only is endo-isopeptidase and lysozyme, but also has antifungal and antibacterial activities. Muramidase activity of rec. Dest-Eys disappeared after heat-treating at 90~C for 50 min without the loss of its antimicrobial activity. Furthermore we showed for the first time that the heated-up derivative ofrec.Dest-Lys exhibited more potent activities against the above enumerated fungi and gram-negative bacteria than original protein.展开更多
Objective: Osteoarthritis is a degenerative disease that affects synovial joints. Micro-injuries of articular structures initiate inflammatory processes, leading to persistent pain. Due to various risk factors,osteoar...Objective: Osteoarthritis is a degenerative disease that affects synovial joints. Micro-injuries of articular structures initiate inflammatory processes, leading to persistent pain. Due to various risk factors,osteoarthritis is often diagnosed in multimorbid patients. This makes pain management one of the key challenges, with a consistent need for new therapeutic strategies. Hence, complementary and integrative methods such as hirudotherapy have become increasingly important, even though their mechanisms of action are not entirely understood.Methods: We retrospectively analyzed the longitudinal effect of a single leech application on osteoarthritic joints in a heterogenic cohort of 24 cases with various chronic pain syndromes. We assessed articular pain intensity ratings and movability of the treated joint after one-time leeching for up to 12 months.We further investigated the effect of hirudotherapy on the systemic pain status and multimodal treatment strategies of the patients.Results: There was a significant reduction in pain intensity ratings at the joint of leech application for up to 12 months after treatment. The improvements in pain intensities were independent of the form of osteoarthritis treated. In addition, we saw a considerable enhancement in local movability of the treated joint. Hirudotherapy did not seem to influence the systemic pain status as well as the previously established individualized multimodal treatment model of the patients.Conclusion: Leeching as an adjuvant therapy has a great potential especially in terms of safety and longterm outcome.展开更多
文摘[Objectives] To identify the species of Hirudo from different sources. [Methods] In accordance with morphological, anatomical and molecular taxonomic characteristics, the identification conclusion was drawn. [Results] The living samples of Hirudo belong to 1 order, 2 families, 3 genera and 5 species, and the dry samples of medicinal leeches belong to 4 species. [Conclusions] The sources of medicinal leeches are complex, and there is a problem that Hirudo from different basic sources are mixed for medication.
文摘Antifungal activity of recombinant medicinal leech destabilase-lysozyme （rec.Dest-Lys） was investi- gated by using fungi： Botrytis cinerea, and Verticillium lateriticum, including yeasts Candida guillermoudii and Shizosaccharomyces pombe. Its antibacterial activity was investigated on gram-negative bacteria Pseudomonas fluorescens. These activities were assessed by radial agar diffusion assay, and scanning and transmission electron microscopy. Therefore, destabilase-lysozyme not only is endo-isopeptidase and lysozyme, but also has antifungal and antibacterial activities. Muramidase activity of rec. Dest-Eys disappeared after heat-treating at 90~C for 50 min without the loss of its antimicrobial activity. Furthermore we showed for the first time that the heated-up derivative ofrec.Dest-Lys exhibited more potent activities against the above enumerated fungi and gram-negative bacteria than original protein.
基金the departmental funding of the Department of Anaesthesiology and Intensive Care Medicine of the University Hospital Cologne。
文摘Objective: Osteoarthritis is a degenerative disease that affects synovial joints. Micro-injuries of articular structures initiate inflammatory processes, leading to persistent pain. Due to various risk factors,osteoarthritis is often diagnosed in multimorbid patients. This makes pain management one of the key challenges, with a consistent need for new therapeutic strategies. Hence, complementary and integrative methods such as hirudotherapy have become increasingly important, even though their mechanisms of action are not entirely understood.Methods: We retrospectively analyzed the longitudinal effect of a single leech application on osteoarthritic joints in a heterogenic cohort of 24 cases with various chronic pain syndromes. We assessed articular pain intensity ratings and movability of the treated joint after one-time leeching for up to 12 months.We further investigated the effect of hirudotherapy on the systemic pain status and multimodal treatment strategies of the patients.Results: There was a significant reduction in pain intensity ratings at the joint of leech application for up to 12 months after treatment. The improvements in pain intensities were independent of the form of osteoarthritis treated. In addition, we saw a considerable enhancement in local movability of the treated joint. Hirudotherapy did not seem to influence the systemic pain status as well as the previously established individualized multimodal treatment model of the patients.Conclusion: Leeching as an adjuvant therapy has a great potential especially in terms of safety and longterm outcome.
