One case of acute megakaryoblastic leukemia （AMKL） with trisomy 21,trisomy 14 and unmutated GATA1 gene in a phenotypically normal girl was reported.The patient experienced transient myelodysplasia before the onset o...One case of acute megakaryoblastic leukemia （AMKL） with trisomy 21,trisomy 14 and unmutated GATA1 gene in a phenotypically normal girl was reported.The patient experienced transient myelodysplasia before the onset of AMKL.The bone marrow blasts manifested typical morphology of megakaryoblast both by the May-Giemsa staining and under the electronic microscopy.Leukemic cells were positive for CD13,CD33,CD117,CD56,CD38,CD41 and CD61 in flow cytometry analysis.Cytogenetic study showed karyotype of 48,XX,＋14,＋21 in 40% metaphases.Known mutations of GATA1 gene in Down syndrome or acquired trisomy 21 were not detected in this case.展开更多
The effect of 1,25-dihydroxyvitamin D<sub>3</sub> [1,25（OH）<sub>2</sub>D<sub>3</sub>] and13-cis-retinoic acid（RA）on the proliferation of a novel human megakaryoblasticleukemia c...The effect of 1,25-dihydroxyvitamin D<sub>3</sub> [1,25（OH）<sub>2</sub>D<sub>3</sub>] and13-cis-retinoic acid（RA）on the proliferation of a novel human megakaryoblasticleukemia cell line（HIMeg）was investigated.At the concentration of 10<sup>9</sup> 10<sup>6</sup>mol/L,1,25（OH）<sub>2</sub>D<sub>3</sub> and RA showed significant inhibition of the proliferation of themegakaryoblastic leukemic cells,which was demonstrated by the count of survival cells,incorporation of<sup>3</sup>H-TdR and<sup>3</sup>H-UR,and cloning efficiency in dose-dependent and time-dependent manners.The results can further explain the mechanism of differentiation-inducing agents and the effect of 1 ,25（OH）<sub>2</sub>D<sub>3</sub> on myelofibrosis.It is possible for1,25（OH）<sub>2</sub>D<sub>3</sub> and RA to be used to treat malignant megakaryocytic diseases.展开更多
Our previous studies revealed that 1, 25-dihydroxyvrtamin D_3[1, 25 (OH)_2, D_3] and its two novel analogues (MC903 and EB1089) play an important role in the modulation of proliferation and differentiation of a newly ...Our previous studies revealed that 1, 25-dihydroxyvrtamin D_3[1, 25 (OH)_2, D_3] and its two novel analogues (MC903 and EB1089) play an important role in the modulation of proliferation and differentiation of a newly established human megakaryoblastic leu展开更多
目的探讨更昔洛韦(GCV)治疗人巨细胞病毒(HCMV)感染相关的特发性血小板减少性紫癜的(ITP)应用指征。方法选择血清HCMV DNA PCR和(或)HCMV IgM检测呈阳性ITP患儿46例,采用逆转聚合酶扩增反应(RT-PCR)检测巨核系祖细胞中HCMV晚期抗原mRNA...目的探讨更昔洛韦(GCV)治疗人巨细胞病毒(HCMV)感染相关的特发性血小板减少性紫癜的(ITP)应用指征。方法选择血清HCMV DNA PCR和(或)HCMV IgM检测呈阳性ITP患儿46例,采用逆转聚合酶扩增反应(RT-PCR)检测巨核系祖细胞中HCMV晚期抗原mRNA,并行GCV治疗,判定其疗效及应用指征。结果46例血清HCMV-DNA PCR阳性和(或)血清HCMV-IgM阳性的ITP骨髓巨核祖细胞HCMV晚期抗原基因mRNA阳性19例,GCV治疗有效16例,mRNA阴性27例,GCV治疗有效4例。阳性组疗效高于阴性组,有显著性差异(P<0.01)。结论HCMV感染的ITP患儿,若对常规治疗无效,应检测其巨核祖细胞内HCMV晚期抗原基因mRNA,阳性者是应用更昔洛韦的有效指征。展开更多
文摘One case of acute megakaryoblastic leukemia （AMKL） with trisomy 21,trisomy 14 and unmutated GATA1 gene in a phenotypically normal girl was reported.The patient experienced transient myelodysplasia before the onset of AMKL.The bone marrow blasts manifested typical morphology of megakaryoblast both by the May-Giemsa staining and under the electronic microscopy.Leukemic cells were positive for CD13,CD33,CD117,CD56,CD38,CD41 and CD61 in flow cytometry analysis.Cytogenetic study showed karyotype of 48,XX,＋14,＋21 in 40% metaphases.Known mutations of GATA1 gene in Down syndrome or acquired trisomy 21 were not detected in this case.
文摘The effect of 1,25-dihydroxyvitamin D<sub>3</sub> [1,25（OH）<sub>2</sub>D<sub>3</sub>] and13-cis-retinoic acid（RA）on the proliferation of a novel human megakaryoblasticleukemia cell line（HIMeg）was investigated.At the concentration of 10<sup>9</sup> 10<sup>6</sup>mol/L,1,25（OH）<sub>2</sub>D<sub>3</sub> and RA showed significant inhibition of the proliferation of themegakaryoblastic leukemic cells,which was demonstrated by the count of survival cells,incorporation of<sup>3</sup>H-TdR and<sup>3</sup>H-UR,and cloning efficiency in dose-dependent and time-dependent manners.The results can further explain the mechanism of differentiation-inducing agents and the effect of 1 ,25（OH）<sub>2</sub>D<sub>3</sub> on myelofibrosis.It is possible for1,25（OH）<sub>2</sub>D<sub>3</sub> and RA to be used to treat malignant megakaryocytic diseases.
文摘Our previous studies revealed that 1, 25-dihydroxyvrtamin D_3[1, 25 (OH)_2, D_3] and its two novel analogues (MC903 and EB1089) play an important role in the modulation of proliferation and differentiation of a newly established human megakaryoblastic leu
文摘目的探讨更昔洛韦(GCV)治疗人巨细胞病毒(HCMV)感染相关的特发性血小板减少性紫癜的(ITP)应用指征。方法选择血清HCMV DNA PCR和(或)HCMV IgM检测呈阳性ITP患儿46例,采用逆转聚合酶扩增反应(RT-PCR)检测巨核系祖细胞中HCMV晚期抗原mRNA,并行GCV治疗,判定其疗效及应用指征。结果46例血清HCMV-DNA PCR阳性和(或)血清HCMV-IgM阳性的ITP骨髓巨核祖细胞HCMV晚期抗原基因mRNA阳性19例,GCV治疗有效16例,mRNA阴性27例,GCV治疗有效4例。阳性组疗效高于阴性组,有显著性差异(P<0.01)。结论HCMV感染的ITP患儿,若对常规治疗无效,应检测其巨核祖细胞内HCMV晚期抗原基因mRNA,阳性者是应用更昔洛韦的有效指征。
