Petrography and Mineralography of Emarat Lead and Zinc Deposit (South Arak)

Iran is host to more than 285 zinc and lead carbonated host deposits including world-class deposits such as Mehdi Abad and Irankouh. Emarat deposit is located in Sanandaj-Sirjan zone and Malayer-Isfahan metallogenic province (northwestern part of Sanandaj-Sirjan tectonic zone). In the area, the mineralization has stratabound shape and restricted to Early Cretaceous limestones and dolomites. With investigation of the optical properties in microscopic survey and evaluation results of analysis, sphalerite, galena, pyrite and chalcopyrite were recognized as the main minerals, covellite, tennantite-tetrahydrite and serosities, quartz and barite as the secondary minerals and gyps, smithsonite were recognized as the minorminerals in the samples. Silicification alteration acted as major process in studied deposit and dolomitization, pyritization and hematitization also were observed in the deposit. Evidences show that mineralization has occurred in post diagenetic stage (epigenetic type) and after tectonic influence.

羊梅迪-维斯那病毒实时荧光定量PCR检测方法的建立

为了建立一种能快速检羊梅迪-维斯那病毒(MVV)的实时荧光定量PCR,根据GenBank中MVV gag基因的序列,设计了1对特异性引物及TaqMan探针,经过反应体系及条件优化,以定量的10倍系列稀释的阳性质粒为标准品进行实时荧光定量PCR扩增,建立了MVV实时荧光定量PCR。结果显示,该方法对MVV DNA最小检出量为10copies/μL,比普通PCR具有较高的检出率;组内及组间变异系数均低于2%,具有较好的重复性;该方法可以特异地检测到MVV,与其他病毒性样品无交叉反应。被检的92份临床样品中,实时荧光定量PCR和常规PCR的阳性检出率分别为4.3%和3.3%。为羊MVV快速检测和分子流行病学调查提供了一种有效的方法。