The central composite design in the modeling and optimization of catalytic dehydration of ethanol to ethylene was performed to improve the ethylene yield.A total of 20 experiments at random were conducted to investiga...The central composite design in the modeling and optimization of catalytic dehydration of ethanol to ethylene was performed to improve the ethylene yield.A total of 20 experiments at random were conducted to investigate the effect of reaction temperature,Si/Al ratios of H-ZSM-5 catalyst and liquid hourly space velocity(LHSV) on the ethylene yield.The results show that the relationship between ethylene yield and the three significant independent variables can be approximated by a nonlinear polynomial model,with R-squared of 99.9%and adjusted R-squared of 99.8%.The maximal response for ethylene yield is 93.4%under the optimal condition of 328 ℃,Si/Al ratio 85,and LHSV 3.8 h-1.展开更多
Different multidisciplinary design optimization (MDO) problems are formulated and compared. Two MDO formulations are applied to a sounding rocket in order to optimize the performance of the rocket. In the MDO of the...Different multidisciplinary design optimization (MDO) problems are formulated and compared. Two MDO formulations are applied to a sounding rocket in order to optimize the performance of the rocket. In the MDO of the referred vehicle, three disciplines have been considered, which are trajectory, propulsion and aerodynamics. A special design structure matrix is developed to assist data exchange between disciplines. This design process uses response surface method (RSM) for multidisciplinary optimization of the rocket. The RSM is applied to the design in two categories: the propulsion model and the system level. In the propulsion model, RSM determines an approximate mathematical model of the engine output parameters as a function of design variables. In the system level, RSM fits a surface of objective function versus design variables. In the first MDO problem formulation, two design variables are selected to form propulsion discipline. In the second one, three new design variables from geometry are added and finally, an optimization method is applied to the response surface in the system level in order to find the best result. Application of the first developed multidisciplinary design optimization procedure increased accessible altitude (performance index) of the referred sounding rocket by twenty five percents and the second one twenty nine.展开更多
The feasibility of copper smelter slag processing by ammonia solution treatment was investigated. The central composite rotatable design(CCRD) and approximation method were used to determine the optimum conditions of ...The feasibility of copper smelter slag processing by ammonia solution treatment was investigated. The central composite rotatable design(CCRD) and approximation method were used to determine the optimum conditions of zinc and copper recovery to a solution. The experimental design was done at five levels of the four operating parameters which were the initial concentration of NH–3, the initial Cl ions concentration, leaching time and solid/liquid ratio. Two mathematical models describing dependence of metal recovery on the operating parameters were obtained. The models are successful in predicting the responses. It was found that optimal parameters for zinc and copper recovery are as follows(values for copper are given in brackets): initial CNH3 17.1%(19.9%), initial CCl– 160 g/L(160 g/L), leaching process duration 4.56 h(4.13 h), solid/liquid ratio 0.39(0.53). The maximum Zn and Cu recoveries to solution, obtained experimentally under the conditions, are 81.16% and 56.48%, respectively.展开更多
Food-grade tracers have been developed as an identification technology for grain traceability from original harvest to final destination for transportation.The characteristics of food-grade tracers must be able to sat...Food-grade tracers have been developed as an identification technology for grain traceability from original harvest to final destination for transportation.The characteristics of food-grade tracers must be able to satisfy the environmental demands for grain traceability.To optimize the food-grade tracer production process,the effects of direct compression formulation and load on the mechanical characteristics were studied using response surface methodology(RSM)with central composite design(CCD).Among the four tested formulations,Formulations#2(consisting of 35.00%lactose 100 mesh,64.50%microcrystalline cellulose 102 and 0.50%magnesium stearate)and#4(consisting of 38.00%lactose 100 mesh,50.00%microcrystalline cellulose 102,11.00%pregelatinized starch and 1.00%magnesium stearate)were selected for tracer production based on their physical properties as powders.The value of Carr’s flowability index was 68 for both Formulations#2 and#4,which was the highest among all the formulations.Therefore,Formulations#2 and#4 also had the best powder flowability.The magnesium stearate ratio(1.00%-3.00%)and pressure(6.00-16.00 kgf)were used as independent variables to detect changes in the breaking rate,peak shear force and friction coefficient of tracers compressed by the selected formulations.The optimal production parameters could be achieved at a magnesium stearate ratio of 2.25%and pressure of 16.00 kgf for Formulation#2 and at a magnesium stearate ratio of 1.02%and pressure of 16.00 kgf for Formulation#4.Under these optimal conditions,the tracers had good impact characteristics(breaking rate),compression characteristics(peak shear force)and frictional characteristics(friction coefficient).Moreover,Formulation#2 was more suitable for production because compared to Formulation#4,its breaking rate and friction coefficient values were lower,and its peak shear force value was higher.展开更多
A strain isolated from the fruiting body of a fungus parasitized on Elaphomyces was identified as Cordyceps ophioglossoides based on the morphological characteristics and the analysis of ITS-5.8s rDNA sequence.The opt...A strain isolated from the fruiting body of a fungus parasitized on Elaphomyces was identified as Cordyceps ophioglossoides based on the morphological characteristics and the analysis of ITS-5.8s rDNA sequence.The optimal medium composition(g·L-1),containing sucrose 66.0,yeast powder 10.0,silkworm chrysalises digest 30.0,MgSO4·7H2O 0.4,and KH2PO4 0.4,was found using fractional factorial design and a central composite design,and the optimization of cultural conditions obtained a result of seed age 6 days,inoculum size 6%(by volume),initial pH 5.6,temperature 24°C,shaking speed 160 r·min-1 by one-factor-at-a-time method.The maximum biomass reached about 20.2 g·L-1 after 90 hours culture under the optimal conditions.Elementary pharmacological activities showed that mycelia of C.ophioglossoides L2 from submerged culture promoted uterus growth in estrogendepleted mice.In the 15-litre scale-up fermentation,the mycelial biomass was around 19.1 g·L-1,indicating a promising prospect for this biotechnology and the potency to develop its medical value.展开更多
An efficient culture medium producing a bacterial elastase with high yields was developed further following preliminary studies by means of response surface method. Central composite design (CCD) and response surface ...An efficient culture medium producing a bacterial elastase with high yields was developed further following preliminary studies by means of response surface method. Central composite design (CCD) and response surface methodology were applied to optimize the medium constituents. A central composite design was used to explain the combined effect of three medium constituents, viz, glucose, K2HPO4, MgSO4·7H2O. The strain produced more elastase in the completely optimized medium, as compared with the partially optimized medium. The fitted model of the second model, as per RSM, showed that glucose was 7.4 g/100 ml, casein 1.13 g/100 ml, corn steep flour 0.616 g/100 ml, KEHPO4 0.206 g/100 ml and MgSO4·7H2O 0.034 g/100 ml. The fermentation kinetics of these two culture media in the flask experiments were analyzed. It was found that the highest elastase productivity occurred at 54 hours. Higher glucose concentration had inhibitory effect on elastase production. At the same time, we observed that the glucose consumption rate was slow in the completely optimized medium, which can explain the lag period of the highest elastase production. Some metal ions and surfactant additives also affected elastase production and cell growth.展开更多
In this study, shea residues (<em>Vitellaria paradoxa</em>) dumped in the wild by the units processing almonds into butter were used in the production of activated carbons. Shea nut shells harvested in the...In this study, shea residues (<em>Vitellaria paradoxa</em>) dumped in the wild by the units processing almonds into butter were used in the production of activated carbons. Shea nut shells harvested in the locality of Baktchoro, West Tandjile Division of Chad were used as a precursor for the preparation of activated carbons by chemical activation with phosphoric acid (H<sub>3</sub>PO<sub>4</sub>) and sulphuric acid (H<sub>2</sub>SO<sub>4</sub>). Central Composite Design (CCD) was used to optimize the preparation conditions, and the factors used were concentration of activating agent (1 - 5 M), carbonization temperature (400<span style="white-space:nowrap;"><span style="white-space:nowrap;">°</span></span>C - 700<span style="white-space:nowrap;"><span style="white-space:nowrap;">°</span></span>C) and residence time (30 - 120 min). The studies showed that at optimal conditions the yield was 51.45% and 42.35%, while the iodine number (IN) was 709.45 and 817.36 mg/g for CAK-P (phosphoric acid activated carbon) and CAK-S (sulphuric acid activated carbon) respectively. These two activated carbons (ACs) which were distinguished by their considerable iodine number, were variously characterized by elementary analysis, pH at the point of zero charge (pHpzc), bulk density, moisture content, Boehm titration, Fourier transform infrared spectroscopy, BET adsorption and scanning electron microscopy. These analyses revealed the acidic and microporous nature of CAK-P and CAK-S carbons, which have a specific microporous surface area of 522.55 and 570.65 m<sup>2</sup>·g<sup><span style="white-space:nowrap;"><span style="white-space:nowrap;">−</span></span>1</sup> respectively.展开更多
In this work, sequential optimization strategy, based on statistical designs, was employed to enhance the production of α-amylase by Aspergillus niger ATCC 16404. This statistical study consists of optimizing the fac...In this work, sequential optimization strategy, based on statistical designs, was employed to enhance the production of α-amylase by Aspergillus niger ATCC 16404. This statistical study consists of optimizing the factors that influence the production of α-amylase of A. niger ATCC 16404. Indeed, another statistical study has allowed the selection of 5 factors (pH, starch, yeast extract, “corn steep liquor”, CaCl<sub>2</sub> and salts) affecting both the development of mould (biomass) and that of the enzyme production. The central composite design allows the determination of the optimum of these selected factors and a quadratic model explains the factor reaction. Thus, the “ridge analysis” method, has led to maximizing the experimental reaction. The results indicate that the production rate of α-amylase is maximized in the presence of starch at 8.97 g/l, yeast extract at 2.86 g/l, CaCl<sub>2</sub> at 1.224 g/l, salts (composed of 25% FeSO<sub>4</sub>, 7H<sub>2</sub>O, 25% MnSO<sub>4</sub> and 50% MgCl<sub>2</sub>, 6H<sub>2</sub>O): FeSO<sub>4</sub>, 7H<sub>2</sub>O, MnSO<sub>4</sub> 0.1518 g/l and MgCl<sub>2</sub>, 6H<sub>2</sub>O at 0.3036 g/l. As for the pH, it is maintained at the rate of 5.68.展开更多
The performances of the response surface methodology(RSM)in connection with the Box–Behnken,face central composite or full factorial design(BBD,FCCD or FFD,respectively)were compared for the use in modeling of the Na...The performances of the response surface methodology(RSM)in connection with the Box–Behnken,face central composite or full factorial design(BBD,FCCD or FFD,respectively)were compared for the use in modeling of the NaOH-catalyzed sunflower oil ethanolysis.The influence of temperature,catalyst loading,and ethanol-to-oil molar ratio(EOMR)on fatty acid ethyl esters(FAEE)content was evaluated.All three multivariate strategies were efficient in the statistical modeling and optimization of the influential process variables but BBD and FCCD realization involved less number of experiments,generating smaller costs,requiring less work and consuming shorter time than the corresponding FFD.All three designs resulted in the same optimal catalyst loading(1.25%of oil)and EOMR(12:1).The reduced two-factorinteraction(2 FI)models based on the BBD and FCCD defined a range of optimal reaction temperature(25℃–75℃)and 25℃,respectively while the same model based on the 33 FFD appointed 75℃.The predicted FAEE content of about 97%–98.0%was close to the experimentally obtained FAEE content of about 97.0%–97.6%under the optimal reaction conditions.Therefore,the simpler BBD or FCCD might successfully be applied for statistical modeling of biodiesel production processes instead of the more extensive,more laborious and more expensive FFD.展开更多
Water in diesel nano-emulsion (WiDNE) due to their nano size, kinetically stable gives its beneficial in commercial and environmental aspects. However, the capability of this fuel strongly depends on the method of pre...Water in diesel nano-emulsion (WiDNE) due to their nano size, kinetically stable gives its beneficial in commercial and environmental aspects. However, the capability of this fuel strongly depends on the method of preparation, stability and their physic-chemical properties. Central composite design (CCD) method was used to optimize variable interactions in order to obtain maximum stability. Methodology RSM method with six independent variables was selected in order to understand the impacts on droplet size. The response surface and 3D plots of the quadratic polynomial model were created for studying the combination effect on response. Dynamic light scattering DLS technique was used for measuring of droplet sizes. The analysis result by ANOVA was with 95% confidence displaying F value model was 52.82. The results displayed model was fulfilled with the assumptions of ANOVA. This study has relied on Design Expert software to locate the optimum droplet size situations. The measured diameter is 26 nm, with 0.0297 errors between actual conditions and measured value. The optimum blend properties of prepared WiDNE fuel were compared with conventional diesel. Improvements in physical properties were observed in presence of water in WiDNE.展开更多
The mathematical models are developed to evaluate the ultimate tensile strength( UTS) and hardness of CNTs / Al2024 composites fabricated by high-energy ball milling. The effects of the preparation variables which are...The mathematical models are developed to evaluate the ultimate tensile strength( UTS) and hardness of CNTs / Al2024 composites fabricated by high-energy ball milling. The effects of the preparation variables which are milling time,rotational speed,mass fraction of CNTs and ball to powder ratio on UST and hardness of CNTs / Al2024 composites are investigated. Based on the central composite design( CCD),a quadratic model is developed to correlate the fabrication variables to the UST and hardness. From the analysis of variance( ANOVA),the most influential factor on each experimental design response is identified. The optimum conditions for preparing CNTs / Al2024 composites are found as follows: 1. 53 h milling time,900 r / min rotational speed,mass fraction of CNTs 2. 87% and Ball to powder ratio 25 ∶ 1. The predicted maximum UST and hardness are 273.30 MPa and 261.36 HV,respectively. And the experimental values are 283.25 MPa and256.8 HV,respectively. It is indicated that the predicted UST and hardness after process optimization are found to agree satisfactory with the experimental values.展开更多
Atrazine, a widely used herbicide, is increasing the agricultural production effectively, while also causing great environmental concern. Efficient atrazine-degrading bacterium is necessary to removal atrazine rapidly...Atrazine, a widely used herbicide, is increasing the agricultural production effectively, while also causing great environmental concern. Efficient atrazine-degrading bacterium is necessary to removal atrazine rapidly to keep a safe environment. In the present study, a new atrazine-degrading strain ZXY-1, identified as Pseudomonas, was isolated. This new isolated strain has a strong ability to biodegrade atrazine with a high efficiency of 9.09 mg/L/hr.Temperature, p H, inoculum size and initial atrazine concentration were examined to further optimize the degradation of atrazine, and the synthetic effect of these factors were investigated by the response surface methodology. With a high quadratic polynomial mathematical model(R^2= 0.9821) being obtained, the highest biodegradation efficiency of 19.03 mg/L/hr was reached compared to previous reports under the optimal conditions(30.71°C, pH 7.14, 4.23%(V/V) inoculum size and 157.1 mg/L initial atrazine concentration).Overall, this study provided an efficient bacterium and approach that could be potentially useful for the bioremediation of wastewater containing atrazine.展开更多
A process was proposed to convert and separate selenium and arsenic in copper anode slime(CAS) by low-temperature alkali fusion process.Central composite design was employed to optimize the effective parameters,in whi...A process was proposed to convert and separate selenium and arsenic in copper anode slime(CAS) by low-temperature alkali fusion process.Central composite design was employed to optimize the effective parameters,in which Na OH/CAS mass ratio,fusion temperature and fusion time were selected as variables,and the conversion ratio of selenium and arsenic as responses.Second-order polynomial models of high significance and 3D response surface plots were constructed to show the relationship between the responses and the variables.Optimum area of >90% selenium conversion ratio and >90% arsenic conversion ratio was obtained by the overlaid contours at Na OH/CAS mass ratio of 0.65-0.75,fusion temperature of 803-823 K and fusion time of 20-30 min.The models are validated by experiments in the optimum area,and the results demonstrate that these models are reliable and accurate in predicting the fusion process.展开更多
BACKGROUND: In this study, we optimized the process for enhancing amylase production from Pseudomonas balearica VITPS 19 isolated from agricultural lands in Kolathur, India. METHODS: Process optimization for enhanci...BACKGROUND: In this study, we optimized the process for enhancing amylase production from Pseudomonas balearica VITPS 19 isolated from agricultural lands in Kolathur, India. METHODS: Process optimization for enhancing amylase production from the isolate was carried out by Response Surface Methodology (RSM) with optimized chemical and physical sources using Design expert v.7.0. A central composite design was used to evaluate the interaction between parameters. Interaction between four factors - maltose (C-source), malt extract (N- source), pH, and CaCl2 was studied. RESULTS: The factors pH and CaCl2 concentration were found to affect amylase production. Validation of the experiment showed a nearly twofold increase in alpha amylase production. CONCLUSION: Amylase production was thus optimized and increased yield was achieved.展开更多
Mango (Mangifera indica L.) and banana (Musa acuminata) are the most popular fruits in the world and widely cultivated crops in the tropical and subtropical zones. Keitt mangoes and Cavendish bananas are the largest c...Mango (Mangifera indica L.) and banana (Musa acuminata) are the most popular fruits in the world and widely cultivated crops in the tropical and subtropical zones. Keitt mangoes and Cavendish bananas are the largest cultivar of these fruits found in the Mozambique market. They are only available for a short period each year mostly during the late summer and early falls. Due to mango and banana fruits high water activity and respiration rate, are perishable foods and require conservation methods for preservation and availability. The aim of this study was to optimize the hot water-calcium chloride concentration treatment regime for improved postharvest handling of mangoes and bananas. The fruits collected were of uniform size, and at greenyellowish maturity stage based on length, diameter, colour and firmness. The process was optimized by experimental central composite design using hot water temperature (50°C - 60°C) and calcium chloride concentration (2% - 4%) with the aid of desirability function. The samples were analyzed for the centesimal composition, firmness, colour, °Brix, Aw, pH, titratable acidity and vitamin C. The results showed that hot water temperature and calcium chloride concentration were influent on the Keitt mangoes b* colour attribute, pH and titratable acidity as well as the Cavendish bananas firmness, ash and vitamin C content. The optimal conditions of the process were stabilized with the desirable function and, coincidentally for both crops, obtained at 55°C of hot water temperature dipping and 3% of calcium chloride concentration. The simulated data were similar to the experimental ones. This is the first time that calcium chloride-hot water treatment is being reported as a means of extending the shelf-life of mangoes and bananas.展开更多
Response surface methodology (RSM) based on desirability function approach (DFA) is applied to obtain an optimal design of the impeller geometry for an automotive torque converter. <span style="font-family:Ver...Response surface methodology (RSM) based on desirability function approach (DFA) is applied to obtain an optimal design of the impeller geometry for an automotive torque converter. <span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">The relative importance of six design parameters including impeller blade number, blade thickness, bias angle, scroll angle, inlet angle and exit angle is investigated using orthogonal design approach. </span></span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">The impeller inlet angle, exit angle and bias angle </span><span style="font-family:Verdana;">are found to exert the greatest influence on the overall performance of a torque converter, with two flow area factors being considered, namely 17% and 20%. Then, RSM together with central composite design (CCD) method is used to in-depth evaluate the interaction effect of the three key parameters on converter performance. The results demonstrate that </span><span style="font-family:Verdana;">impeller exit angle has the strongest impact on peak efficiency</span><span style="font-family:Verdana;">, with larger angles yielding the most favorable results. The stall torque ratio maximization is attainable with the increase of impeller bias angle and inlet angle together with smaller exit angle. In the end, </span><span style="font-family:Verdana;">an optimized design for the impeller geometry is obtained with stall torque ratio and peak efficiency increased by 1.62% and 1.1%, respectively.</span><span style="font-family:Verdana;"> The new optimization method can be used as a reference for performance enhancement in the design process of impeller geometry for an automotive torque converter.</span></span></span></span>展开更多
基金Supported by National Natural Science Foundation of China(No.70671072)
文摘The central composite design in the modeling and optimization of catalytic dehydration of ethanol to ethylene was performed to improve the ethylene yield.A total of 20 experiments at random were conducted to investigate the effect of reaction temperature,Si/Al ratios of H-ZSM-5 catalyst and liquid hourly space velocity(LHSV) on the ethylene yield.The results show that the relationship between ethylene yield and the three significant independent variables can be approximated by a nonlinear polynomial model,with R-squared of 99.9%and adjusted R-squared of 99.8%.The maximal response for ethylene yield is 93.4%under the optimal condition of 328 ℃,Si/Al ratio 85,and LHSV 3.8 h-1.
文摘Different multidisciplinary design optimization (MDO) problems are formulated and compared. Two MDO formulations are applied to a sounding rocket in order to optimize the performance of the rocket. In the MDO of the referred vehicle, three disciplines have been considered, which are trajectory, propulsion and aerodynamics. A special design structure matrix is developed to assist data exchange between disciplines. This design process uses response surface method (RSM) for multidisciplinary optimization of the rocket. The RSM is applied to the design in two categories: the propulsion model and the system level. In the propulsion model, RSM determines an approximate mathematical model of the engine output parameters as a function of design variables. In the system level, RSM fits a surface of objective function versus design variables. In the first MDO problem formulation, two design variables are selected to form propulsion discipline. In the second one, three new design variables from geometry are added and finally, an optimization method is applied to the response surface in the system level in order to find the best result. Application of the first developed multidisciplinary design optimization procedure increased accessible altitude (performance index) of the referred sounding rocket by twenty five percents and the second one twenty nine.
文摘The feasibility of copper smelter slag processing by ammonia solution treatment was investigated. The central composite rotatable design(CCRD) and approximation method were used to determine the optimum conditions of zinc and copper recovery to a solution. The experimental design was done at five levels of the four operating parameters which were the initial concentration of NH–3, the initial Cl ions concentration, leaching time and solid/liquid ratio. Two mathematical models describing dependence of metal recovery on the operating parameters were obtained. The models are successful in predicting the responses. It was found that optimal parameters for zinc and copper recovery are as follows(values for copper are given in brackets): initial CNH3 17.1%(19.9%), initial CCl– 160 g/L(160 g/L), leaching process duration 4.56 h(4.13 h), solid/liquid ratio 0.39(0.53). The maximum Zn and Cu recoveries to solution, obtained experimentally under the conditions, are 81.16% and 56.48%, respectively.
基金This work was supported by the National Natural Science Foundation of China(31401610)the Fundamental Research Funds for the Central Universities of China(KJON201557)+1 种基金the Outstanding Youth Foundation Science and Technology Fund of College of Engineering at Nanjing Agricultural University(YQ201603)the Jiangsu Agriculture Science and Technology Innovation Fund(CX(16)1059).
文摘Food-grade tracers have been developed as an identification technology for grain traceability from original harvest to final destination for transportation.The characteristics of food-grade tracers must be able to satisfy the environmental demands for grain traceability.To optimize the food-grade tracer production process,the effects of direct compression formulation and load on the mechanical characteristics were studied using response surface methodology(RSM)with central composite design(CCD).Among the four tested formulations,Formulations#2(consisting of 35.00%lactose 100 mesh,64.50%microcrystalline cellulose 102 and 0.50%magnesium stearate)and#4(consisting of 38.00%lactose 100 mesh,50.00%microcrystalline cellulose 102,11.00%pregelatinized starch and 1.00%magnesium stearate)were selected for tracer production based on their physical properties as powders.The value of Carr’s flowability index was 68 for both Formulations#2 and#4,which was the highest among all the formulations.Therefore,Formulations#2 and#4 also had the best powder flowability.The magnesium stearate ratio(1.00%-3.00%)and pressure(6.00-16.00 kgf)were used as independent variables to detect changes in the breaking rate,peak shear force and friction coefficient of tracers compressed by the selected formulations.The optimal production parameters could be achieved at a magnesium stearate ratio of 2.25%and pressure of 16.00 kgf for Formulation#2 and at a magnesium stearate ratio of 1.02%and pressure of 16.00 kgf for Formulation#4.Under these optimal conditions,the tracers had good impact characteristics(breaking rate),compression characteristics(peak shear force)and frictional characteristics(friction coefficient).Moreover,Formulation#2 was more suitable for production because compared to Formulation#4,its breaking rate and friction coefficient values were lower,and its peak shear force value was higher.
基金Supported by the Research Project of Science and Technology of Zhejiang Province, China (2005C23027), the National High Technology Research and Development Program of China (2007AA021506) and the Natural Science Foundation of Zhejiang Province (R207609).
We would like to thank Dr. Birnie from New Zealand for his editing of this manuscript.
文摘A strain isolated from the fruiting body of a fungus parasitized on Elaphomyces was identified as Cordyceps ophioglossoides based on the morphological characteristics and the analysis of ITS-5.8s rDNA sequence.The optimal medium composition(g·L-1),containing sucrose 66.0,yeast powder 10.0,silkworm chrysalises digest 30.0,MgSO4·7H2O 0.4,and KH2PO4 0.4,was found using fractional factorial design and a central composite design,and the optimization of cultural conditions obtained a result of seed age 6 days,inoculum size 6%(by volume),initial pH 5.6,temperature 24°C,shaking speed 160 r·min-1 by one-factor-at-a-time method.The maximum biomass reached about 20.2 g·L-1 after 90 hours culture under the optimal conditions.Elementary pharmacological activities showed that mycelia of C.ophioglossoides L2 from submerged culture promoted uterus growth in estrogendepleted mice.In the 15-litre scale-up fermentation,the mycelial biomass was around 19.1 g·L-1,indicating a promising prospect for this biotechnology and the potency to develop its medical value.
文摘An efficient culture medium producing a bacterial elastase with high yields was developed further following preliminary studies by means of response surface method. Central composite design (CCD) and response surface methodology were applied to optimize the medium constituents. A central composite design was used to explain the combined effect of three medium constituents, viz, glucose, K2HPO4, MgSO4·7H2O. The strain produced more elastase in the completely optimized medium, as compared with the partially optimized medium. The fitted model of the second model, as per RSM, showed that glucose was 7.4 g/100 ml, casein 1.13 g/100 ml, corn steep flour 0.616 g/100 ml, KEHPO4 0.206 g/100 ml and MgSO4·7H2O 0.034 g/100 ml. The fermentation kinetics of these two culture media in the flask experiments were analyzed. It was found that the highest elastase productivity occurred at 54 hours. Higher glucose concentration had inhibitory effect on elastase production. At the same time, we observed that the glucose consumption rate was slow in the completely optimized medium, which can explain the lag period of the highest elastase production. Some metal ions and surfactant additives also affected elastase production and cell growth.
文摘In this study, shea residues (<em>Vitellaria paradoxa</em>) dumped in the wild by the units processing almonds into butter were used in the production of activated carbons. Shea nut shells harvested in the locality of Baktchoro, West Tandjile Division of Chad were used as a precursor for the preparation of activated carbons by chemical activation with phosphoric acid (H<sub>3</sub>PO<sub>4</sub>) and sulphuric acid (H<sub>2</sub>SO<sub>4</sub>). Central Composite Design (CCD) was used to optimize the preparation conditions, and the factors used were concentration of activating agent (1 - 5 M), carbonization temperature (400<span style="white-space:nowrap;"><span style="white-space:nowrap;">°</span></span>C - 700<span style="white-space:nowrap;"><span style="white-space:nowrap;">°</span></span>C) and residence time (30 - 120 min). The studies showed that at optimal conditions the yield was 51.45% and 42.35%, while the iodine number (IN) was 709.45 and 817.36 mg/g for CAK-P (phosphoric acid activated carbon) and CAK-S (sulphuric acid activated carbon) respectively. These two activated carbons (ACs) which were distinguished by their considerable iodine number, were variously characterized by elementary analysis, pH at the point of zero charge (pHpzc), bulk density, moisture content, Boehm titration, Fourier transform infrared spectroscopy, BET adsorption and scanning electron microscopy. These analyses revealed the acidic and microporous nature of CAK-P and CAK-S carbons, which have a specific microporous surface area of 522.55 and 570.65 m<sup>2</sup>·g<sup><span style="white-space:nowrap;"><span style="white-space:nowrap;">−</span></span>1</sup> respectively.
文摘In this work, sequential optimization strategy, based on statistical designs, was employed to enhance the production of α-amylase by Aspergillus niger ATCC 16404. This statistical study consists of optimizing the factors that influence the production of α-amylase of A. niger ATCC 16404. Indeed, another statistical study has allowed the selection of 5 factors (pH, starch, yeast extract, “corn steep liquor”, CaCl<sub>2</sub> and salts) affecting both the development of mould (biomass) and that of the enzyme production. The central composite design allows the determination of the optimum of these selected factors and a quadratic model explains the factor reaction. Thus, the “ridge analysis” method, has led to maximizing the experimental reaction. The results indicate that the production rate of α-amylase is maximized in the presence of starch at 8.97 g/l, yeast extract at 2.86 g/l, CaCl<sub>2</sub> at 1.224 g/l, salts (composed of 25% FeSO<sub>4</sub>, 7H<sub>2</sub>O, 25% MnSO<sub>4</sub> and 50% MgCl<sub>2</sub>, 6H<sub>2</sub>O): FeSO<sub>4</sub>, 7H<sub>2</sub>O, MnSO<sub>4</sub> 0.1518 g/l and MgCl<sub>2</sub>, 6H<sub>2</sub>O at 0.3036 g/l. As for the pH, it is maintained at the rate of 5.68.
基金Supported jointly by the Ministry of Education,485 Science and Technological Development of the Republic of Serbia(Project 4Q8614 Ⅲ 45001)a part of the Project 0-14-18 of the SASA Branch in Nis 487(Development,modeling and optimization of biodiesel production from 4Q8815 nonedible and waste feedstocks),Serbia
文摘The performances of the response surface methodology(RSM)in connection with the Box–Behnken,face central composite or full factorial design(BBD,FCCD or FFD,respectively)were compared for the use in modeling of the NaOH-catalyzed sunflower oil ethanolysis.The influence of temperature,catalyst loading,and ethanol-to-oil molar ratio(EOMR)on fatty acid ethyl esters(FAEE)content was evaluated.All three multivariate strategies were efficient in the statistical modeling and optimization of the influential process variables but BBD and FCCD realization involved less number of experiments,generating smaller costs,requiring less work and consuming shorter time than the corresponding FFD.All three designs resulted in the same optimal catalyst loading(1.25%of oil)and EOMR(12:1).The reduced two-factorinteraction(2 FI)models based on the BBD and FCCD defined a range of optimal reaction temperature(25℃–75℃)and 25℃,respectively while the same model based on the 33 FFD appointed 75℃.The predicted FAEE content of about 97%–98.0%was close to the experimentally obtained FAEE content of about 97.0%–97.6%under the optimal reaction conditions.Therefore,the simpler BBD or FCCD might successfully be applied for statistical modeling of biodiesel production processes instead of the more extensive,more laborious and more expensive FFD.
文摘Water in diesel nano-emulsion (WiDNE) due to their nano size, kinetically stable gives its beneficial in commercial and environmental aspects. However, the capability of this fuel strongly depends on the method of preparation, stability and their physic-chemical properties. Central composite design (CCD) method was used to optimize variable interactions in order to obtain maximum stability. Methodology RSM method with six independent variables was selected in order to understand the impacts on droplet size. The response surface and 3D plots of the quadratic polynomial model were created for studying the combination effect on response. Dynamic light scattering DLS technique was used for measuring of droplet sizes. The analysis result by ANOVA was with 95% confidence displaying F value model was 52.82. The results displayed model was fulfilled with the assumptions of ANOVA. This study has relied on Design Expert software to locate the optimum droplet size situations. The measured diameter is 26 nm, with 0.0297 errors between actual conditions and measured value. The optimum blend properties of prepared WiDNE fuel were compared with conventional diesel. Improvements in physical properties were observed in presence of water in WiDNE.
基金Sponsored by the Program for Innovative Research Team in University of Yunnan Province and Major Projects of Yunnan Province(Grant No.2014FC001)
文摘The mathematical models are developed to evaluate the ultimate tensile strength( UTS) and hardness of CNTs / Al2024 composites fabricated by high-energy ball milling. The effects of the preparation variables which are milling time,rotational speed,mass fraction of CNTs and ball to powder ratio on UST and hardness of CNTs / Al2024 composites are investigated. Based on the central composite design( CCD),a quadratic model is developed to correlate the fabrication variables to the UST and hardness. From the analysis of variance( ANOVA),the most influential factor on each experimental design response is identified. The optimum conditions for preparing CNTs / Al2024 composites are found as follows: 1. 53 h milling time,900 r / min rotational speed,mass fraction of CNTs 2. 87% and Ball to powder ratio 25 ∶ 1. The predicted maximum UST and hardness are 273.30 MPa and 261.36 HV,respectively. And the experimental values are 283.25 MPa and256.8 HV,respectively. It is indicated that the predicted UST and hardness after process optimization are found to agree satisfactory with the experimental values.
基金supported by the Major Science and Technology Program for Water Pollution Control and Treatment(No.2012ZX07212001)the National Natural Science Foundation of China(No.31570505)the State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology(No.2014TS05)
文摘Atrazine, a widely used herbicide, is increasing the agricultural production effectively, while also causing great environmental concern. Efficient atrazine-degrading bacterium is necessary to removal atrazine rapidly to keep a safe environment. In the present study, a new atrazine-degrading strain ZXY-1, identified as Pseudomonas, was isolated. This new isolated strain has a strong ability to biodegrade atrazine with a high efficiency of 9.09 mg/L/hr.Temperature, p H, inoculum size and initial atrazine concentration were examined to further optimize the degradation of atrazine, and the synthetic effect of these factors were investigated by the response surface methodology. With a high quadratic polynomial mathematical model(R^2= 0.9821) being obtained, the highest biodegradation efficiency of 19.03 mg/L/hr was reached compared to previous reports under the optimal conditions(30.71°C, pH 7.14, 4.23%(V/V) inoculum size and 157.1 mg/L initial atrazine concentration).Overall, this study provided an efficient bacterium and approach that could be potentially useful for the bioremediation of wastewater containing atrazine.
基金Project(51234009)supported by the National Natural Science Foundation of ChinaProject(2014DFA90520)supported by International Cooperation Program of Ministry of Science of ChinaProject(2013A100003)supported by the Production,Teaching and Research Program of Guangdong Province,China
文摘A process was proposed to convert and separate selenium and arsenic in copper anode slime(CAS) by low-temperature alkali fusion process.Central composite design was employed to optimize the effective parameters,in which Na OH/CAS mass ratio,fusion temperature and fusion time were selected as variables,and the conversion ratio of selenium and arsenic as responses.Second-order polynomial models of high significance and 3D response surface plots were constructed to show the relationship between the responses and the variables.Optimum area of >90% selenium conversion ratio and >90% arsenic conversion ratio was obtained by the overlaid contours at Na OH/CAS mass ratio of 0.65-0.75,fusion temperature of 803-823 K and fusion time of 20-30 min.The models are validated by experiments in the optimum area,and the results demonstrate that these models are reliable and accurate in predicting the fusion process.
文摘BACKGROUND: In this study, we optimized the process for enhancing amylase production from Pseudomonas balearica VITPS 19 isolated from agricultural lands in Kolathur, India. METHODS: Process optimization for enhancing amylase production from the isolate was carried out by Response Surface Methodology (RSM) with optimized chemical and physical sources using Design expert v.7.0. A central composite design was used to evaluate the interaction between parameters. Interaction between four factors - maltose (C-source), malt extract (N- source), pH, and CaCl2 was studied. RESULTS: The factors pH and CaCl2 concentration were found to affect amylase production. Validation of the experiment showed a nearly twofold increase in alpha amylase production. CONCLUSION: Amylase production was thus optimized and increased yield was achieved.
文摘Mango (Mangifera indica L.) and banana (Musa acuminata) are the most popular fruits in the world and widely cultivated crops in the tropical and subtropical zones. Keitt mangoes and Cavendish bananas are the largest cultivar of these fruits found in the Mozambique market. They are only available for a short period each year mostly during the late summer and early falls. Due to mango and banana fruits high water activity and respiration rate, are perishable foods and require conservation methods for preservation and availability. The aim of this study was to optimize the hot water-calcium chloride concentration treatment regime for improved postharvest handling of mangoes and bananas. The fruits collected were of uniform size, and at greenyellowish maturity stage based on length, diameter, colour and firmness. The process was optimized by experimental central composite design using hot water temperature (50°C - 60°C) and calcium chloride concentration (2% - 4%) with the aid of desirability function. The samples were analyzed for the centesimal composition, firmness, colour, °Brix, Aw, pH, titratable acidity and vitamin C. The results showed that hot water temperature and calcium chloride concentration were influent on the Keitt mangoes b* colour attribute, pH and titratable acidity as well as the Cavendish bananas firmness, ash and vitamin C content. The optimal conditions of the process were stabilized with the desirable function and, coincidentally for both crops, obtained at 55°C of hot water temperature dipping and 3% of calcium chloride concentration. The simulated data were similar to the experimental ones. This is the first time that calcium chloride-hot water treatment is being reported as a means of extending the shelf-life of mangoes and bananas.
文摘Response surface methodology (RSM) based on desirability function approach (DFA) is applied to obtain an optimal design of the impeller geometry for an automotive torque converter. <span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">The relative importance of six design parameters including impeller blade number, blade thickness, bias angle, scroll angle, inlet angle and exit angle is investigated using orthogonal design approach. </span></span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">The impeller inlet angle, exit angle and bias angle </span><span style="font-family:Verdana;">are found to exert the greatest influence on the overall performance of a torque converter, with two flow area factors being considered, namely 17% and 20%. Then, RSM together with central composite design (CCD) method is used to in-depth evaluate the interaction effect of the three key parameters on converter performance. The results demonstrate that </span><span style="font-family:Verdana;">impeller exit angle has the strongest impact on peak efficiency</span><span style="font-family:Verdana;">, with larger angles yielding the most favorable results. The stall torque ratio maximization is attainable with the increase of impeller bias angle and inlet angle together with smaller exit angle. In the end, </span><span style="font-family:Verdana;">an optimized design for the impeller geometry is obtained with stall torque ratio and peak efficiency increased by 1.62% and 1.1%, respectively.</span><span style="font-family:Verdana;"> The new optimization method can be used as a reference for performance enhancement in the design process of impeller geometry for an automotive torque converter.</span></span></span></span>