Cytotoxicity screening of Melastoma malabathricum extracts on human breast cancer cell lines in vitro

Objective:To screen the cytotoxic activity of Melasloma malabathricum(M,malubathricum)against human breast carreer cell line(MCF-7)in vitro.Methods:A three steps extraction protocol using n-hexane,chloroform and methanol as the solvents systems was carried out on leaves,stems and flowers of M.nalabathricum.Dimethyl sulfoxide was used in extracts dilution and serial dilutions were conducted to obtain five different extract concentrations(100μg/mL,50μg/mL,25μg/mL,123μg/rnL and 6.25μg/mL).The evaluation of cell growth was determined using methylene blue assay.Results:Methanol extract from the leaves showed significant anticancer activity against MCF-7cell lines with the TC_(50)value of 7.14μg/ml while methanol and chloroform extract from the flowers exhibited a moderate activity towards MCF-7 cell line,with the IC_(50)value of 33.63μg/mL and 45.76μg/mL respectively after 72 h of treatment.Conclusions:The extracts from leaves and flowers of M.nulabatkricum showed promising anticancer activity toward human breast cancer cell lines with the lowest IC_(50)at 7.14μg/mL while the extracts from stems showed less growth inhibition activity.

Potentials of <i>Melastoma malabathricum</i>Linn. Flower and Fruit Extracts as Antimicrobial Infusions

Melastoma malabathricum Linn. is a shrub that belongs to the family Melastomataceae and a common herbal plant used in folk medicines to treat inflamed wounds. This study was carried out with the aim to evaluate the inhibitory activities of different concentrations of the M. malabathricum Linn. flower and fruit crude extracts against a variety of microorganisms. The inhibitory effects of both extracts were tested against the microorganisms using the disc diffusion method. The lowest concentrations of the extracts producing inhibition zones against the test microorganisms were used to determine their Minimum Inhibitory Concentrations (MICs) and Minimum Microbicidal Concentrations (MMCs). Both crude extracts showed strong inhibitory activities against Gram-positive bacteria. The range of MIC values for the crude flower and fruit extracts on all the bacteria tested were 12.5 to 100.0 mg/ml. Overall, Gram-positive bacteria were more susceptible to the crude extracts compared to Gram-negative species, potentiating a possible use of the extracts to inhibit or kill potential pathogens.

Thrombocyte counts in mice after the administration of methanolic extract of Melastoma malabathricum

Objective:To investigate the effect of methanolic extract of Melastoma malabathricum(M.malabathricum)in thrombocyte counts in mice.Methods:Methanolic extract of M.malabathricum corresponding to 1.5 to 2 mg/10 g body weight in saline was administered to mices via oral route.Control group was given normal saline.Twenty five microlitres of blood were drawn at 0 h and thereafterat 1,2,4,8,12,20 h after dosing via tail bleeding technique.The thrombocytes were counted in the triple laminated middle 25 squares of the haemocytometer using light microscope.Results:The control group showed a moderate rise after the administration of saline whereas the M.malabathricum treated group shows significant rise within 2nd h in thrombocyte counts with an increment of 51.64%compared to baseline count.Conclusions:Based on the results,it can be concluded that M.malabathricum could be a potential remedy in treating thrombocytopenic condition.

野牡丹异型雄蕊的功能分化

野牡丹科植物具有形态、大小和颜色显著不同的两种异型雄蕊。关于其异型雄蕊是否具有功能分化还一直存在争论。本文以野牡丹科植物野牡丹(Melastoma malabathricum)为实验材料,比较了两种异型雄蕊在传粉过程中的功能作用。结果表明,两种异型雄蕊在形态、花粉量和人工控制实验条件下的结籽数,以及主要传粉昆虫木蜂(Xylocopa sp.)访花时的行为等方面都有显著差异,说明两种雄蕊在传粉过程中存在一定的功能分化:外轮紫色雄蕊中的花粉为后代提供雄配子,而内轮黄色雄蕊中的花粉则为传粉昆虫提供食物。但两种雄蕊在花粉活性、花粉组织化学成分和结实率方面差异均不显著,表明两者在生理上并没有分化。实验结果还表明,除花前套袋不结实外,自交、异交和自然对照都具有较高的结实率,说明野牡丹不存在无融合生殖和主动自交及自交不亲和现象,为兼性异交。

干旱胁迫对3种野牡丹科植株形态和生理特性的影响

以3种野牡丹科植物为材料,研究土壤干旱胁迫对植株形态及生理特性的影响,并用隶属函数法对其抗旱性进行综合评价。结果表明,3种植物的叶片相对电导率和脯氨酸含量随着土壤干旱胁迫程度的增加呈上升趋势;野牡丹‘天骄’和翅茎异形木MDA含量呈上升趋势,在重度干旱胁迫时达到最大值,而角茎野牡丹则表现出先上升后下降趋势,在中度胁迫时达最大值(18.1 μmol/g)。3种植物的可溶性蛋白含量对干旱胁迫不敏感,而可溶性糖含量则表现出先下降后上升趋势,在轻度干旱胁迫时达最小值,在重度干旱胁迫时达最大值。隶属函数分析结果表明,翅茎异形木和野牡丹‘天骄’的耐旱性较强,角茎野牡丹的耐旱性较弱。

不同花色印度野牡丹转录组功能注释和分析

印度野牡丹(Melastoma malabathricum)属于野牡丹科野牡丹属,是双子叶有花植物,具有优良的观赏价值和药用价值,在未来的城乡绿化中具有较大的应用潜力和景观贡献力。本研究以粉色和白色的印度野牡丹为材料,采用RNA-seq转录组测序获得54 725条Unigene,并分别通过Nr、SwissProt、KOG和KEGG 4个数据库分别进行同源比对和功能注释,其中有11 319条Unigenes在4个数据库中都注释到了相应的功能基因。GO注释到123 355个Unigenes分为3个本体49个功能组,KEGG注释到7 880条Unigenes涉及129种代谢途径,其中部分Unigene涉及花色素合成途径、类黄酮合成途径、类胡萝卜素等合成途径。这些研究结果为后续深入开展印度野牡丹花青素等物质代谢合成途径及相关基因研究奠定基础。