To confirm the content and distribution of bioactive components in roots, stems, leaves and grains of Russian Me/i/otus officina/is ＇Sligen 1 ＂, an experiment involving bioactive components detection and field plant...To confirm the content and distribution of bioactive components in roots, stems, leaves and grains of Russian Me/i/otus officina/is ＇Sligen 1 ＂, an experiment involving bioactive components detection and field planting was conducted. Kjeldahl method and high performance gas chromatography were used to detect the protein. The automatic amino acid analyzer was used to detect amino acid. Gas chromatography-mass spectrometry （GC-MS） and molecular distillation apparatus were used to confirm the fatty acid content. Gravimetric method was used to detect saponin and alkaloid contents. High performance liquid chromatography was used to detect the coumarin content. The polyphenol content was determined by forint reagent colorimetric method. Anthracene copper-sulfuric acid colorimetry was used to detect the polysaccharide, and aluminum nitrate-sodium nitrite-sodium hydroxide col- orimetric method was used to confirm flavone. The results showed that protein and amino acid were mainly distributed in leaves and seeds. The fatty acid was mainly distributed in seeds. The saponin was mainly distributed in roots and leave. The polyphenol was mainly distributed in roots, stems and leaves. The alkaloid was mainly distributed in stems, leaves and seeds. Seeds mainly contained coumarin. M. officinalis had little polysaccharide and flavone. The total production of bioactive in- gredients was 3 016.3 kg/hm2. The output of crude protein, amino acids and fatty acids was 1 400.6, 1 246.8, 112.2, respectively. The yields of effective components saponins, alkaloids, polyphenols and coumarin were 97.0, 77.9, 41.7, 40.1 kg/hm2, respectively. After separation, extraction and purification, those components can be used as the natural ingredients to develop medicine, food, chemical and other biotechnological products.展开更多
In this study, the allelopathic effects of 100 g/L water extract of Melilotus offtcinalis Desr. on seed germination and seedling growth of Melilotus offwinalis Desr., Trifolium pretense L. , Medicago sativa L. cv. Vic...In this study, the allelopathic effects of 100 g/L water extract of Melilotus offtcinalis Desr. on seed germination and seedling growth of Melilotus offwinalis Desr., Trifolium pretense L. , Medicago sativa L. cv. Victoria, Echinochloa crusgalli （ L. ） Beauv., Lxer/s chinensis （ Thunb. ） Nakai and Plantago asiatica L. were investigated by biological assay. Dried powder of Melilotus officinalis Desr. was applied to the field in spring and winter to verify the inhibitory effects of Me- lilotus officinalis Desr. on weed biomass in the experimental field and compare the effects between Melilotus offwinalis Desr. and a variety of commonly used chemi- cal herbicides on weed suppression. The results showed that water extract of Melilotm officinalis Desr. posed significant effects on seed germination and seedling growth of Trifolium pretense L. , Echinochloa crusgalli （ L. ） Beauv. , Lxeris chinensis （ Thunb. ） Nakm, Plantago asiatica L. , Melilotus o^tcinalis Desr. and Medi- cago sativa L. cv. Victoria, exhibiting strong allelopathic effects, but the inhibition rate varied greatly among different plant species. Therefore, the allelopathic effects of Melilotus o^winalis Desr. should be utilized appropriately in practical applieatians. Dried powder of Melilotus officinalis Desr. significantly reduced weed quantity in the field for a short duration, which should be administered multiple times in a sufficient amount for weed control in the production.展开更多
To improve the serious situation of soil degradation in China, tests were carried out to study the improvement effects of Russian Melilotus officinalis on the low base saturation acid soil, the alkaline-saline soil an...To improve the serious situation of soil degradation in China, tests were carried out to study the improvement effects of Russian Melilotus officinalis on the low base saturation acid soil, the alkaline-saline soil and the heavy metal contaminated soil. The results showed that M. officinalis could improve the low base saturation acid soil, increase the salt, alkali content, mineral nutrition and organic nutrient, which indicated that M. officinalis could be planted in the acid soil, and had a significant ecological effect on soil improvement. M. officinalis achieved a remarkable effect on improving the alkaline-saline soil （pH 8.5-9.5） that the soil desalted, dealkalized and had more organic nutrient. Moreover, M. officinalis could improve the heavy metal contaminated soil and had the ability to enrich and transform heavy metal elements, especially Cd and Ni, thereby presenting significant repair effects on soil improvement. Therefore, Russian M. officinalis had multiple-effects on degraded soil improvement, so it is worthy of application and popularization.展开更多
In view of Melilotus officinalis having high protein content and significant effect on improving saline alkali soil,a comprehensive experiment about improving saline alkali soil by planting M.officinalis was carried o...In view of Melilotus officinalis having high protein content and significant effect on improving saline alkali soil,a comprehensive experiment about improving saline alkali soil by planting M.officinalis was carried out to study its effects of potential equivalent land and equivalent grain.The results showed that the forage yield was 80 000 kg/hm^2 in the degraded grassland field( average pH of 9.06) after planting M.officinalis.The forage had a crude protein content of 17.50%,so the total content of crude protein was 1 400.64 kg/hm^2,which was equal to 15.562 t of corns produced from 3.557 hm^2 of medium-quality arable lands.Similarly,it was equal to 3.573 t of soybeans produced in 1.786 hm^2 of medium-quality arable lands.Therefore,based on such principle,the comprehensive development of creatures-soil system can acquire the equivalent land and equivalent grain resources.展开更多
文摘To confirm the content and distribution of bioactive components in roots, stems, leaves and grains of Russian Me/i/otus officina/is ＇Sligen 1 ＂, an experiment involving bioactive components detection and field planting was conducted. Kjeldahl method and high performance gas chromatography were used to detect the protein. The automatic amino acid analyzer was used to detect amino acid. Gas chromatography-mass spectrometry （GC-MS） and molecular distillation apparatus were used to confirm the fatty acid content. Gravimetric method was used to detect saponin and alkaloid contents. High performance liquid chromatography was used to detect the coumarin content. The polyphenol content was determined by forint reagent colorimetric method. Anthracene copper-sulfuric acid colorimetry was used to detect the polysaccharide, and aluminum nitrate-sodium nitrite-sodium hydroxide col- orimetric method was used to confirm flavone. The results showed that protein and amino acid were mainly distributed in leaves and seeds. The fatty acid was mainly distributed in seeds. The saponin was mainly distributed in roots and leave. The polyphenol was mainly distributed in roots, stems and leaves. The alkaloid was mainly distributed in stems, leaves and seeds. Seeds mainly contained coumarin. M. officinalis had little polysaccharide and flavone. The total production of bioactive in- gredients was 3 016.3 kg/hm2. The output of crude protein, amino acids and fatty acids was 1 400.6, 1 246.8, 112.2, respectively. The yields of effective components saponins, alkaloids, polyphenols and coumarin were 97.0, 77.9, 41.7, 40.1 kg/hm2, respectively. After separation, extraction and purification, those components can be used as the natural ingredients to develop medicine, food, chemical and other biotechnological products.
基金Supported by Natural Science Foundation of China(31101764)Natural Science Fund for Colleges and Universities in Jiangsu Province(11KJB230004)
文摘In this study, the allelopathic effects of 100 g/L water extract of Melilotus offtcinalis Desr. on seed germination and seedling growth of Melilotus offwinalis Desr., Trifolium pretense L. , Medicago sativa L. cv. Victoria, Echinochloa crusgalli （ L. ） Beauv., Lxer/s chinensis （ Thunb. ） Nakai and Plantago asiatica L. were investigated by biological assay. Dried powder of Melilotus officinalis Desr. was applied to the field in spring and winter to verify the inhibitory effects of Me- lilotus officinalis Desr. on weed biomass in the experimental field and compare the effects between Melilotus offwinalis Desr. and a variety of commonly used chemi- cal herbicides on weed suppression. The results showed that water extract of Melilotm officinalis Desr. posed significant effects on seed germination and seedling growth of Trifolium pretense L. , Echinochloa crusgalli （ L. ） Beauv. , Lxeris chinensis （ Thunb. ） Nakm, Plantago asiatica L. , Melilotus o^tcinalis Desr. and Medi- cago sativa L. cv. Victoria, exhibiting strong allelopathic effects, but the inhibition rate varied greatly among different plant species. Therefore, the allelopathic effects of Melilotus o^winalis Desr. should be utilized appropriately in practical applieatians. Dried powder of Melilotus officinalis Desr. significantly reduced weed quantity in the field for a short duration, which should be administered multiple times in a sufficient amount for weed control in the production.
基金Supported by the Special Fund for Agro-Scientific Research in the Public Interest of China(200903031)the Project of Science and Technology Department of Jilin Province(20070413)
文摘To improve the serious situation of soil degradation in China, tests were carried out to study the improvement effects of Russian Melilotus officinalis on the low base saturation acid soil, the alkaline-saline soil and the heavy metal contaminated soil. The results showed that M. officinalis could improve the low base saturation acid soil, increase the salt, alkali content, mineral nutrition and organic nutrient, which indicated that M. officinalis could be planted in the acid soil, and had a significant ecological effect on soil improvement. M. officinalis achieved a remarkable effect on improving the alkaline-saline soil （pH 8.5-9.5） that the soil desalted, dealkalized and had more organic nutrient. Moreover, M. officinalis could improve the heavy metal contaminated soil and had the ability to enrich and transform heavy metal elements, especially Cd and Ni, thereby presenting significant repair effects on soil improvement. Therefore, Russian M. officinalis had multiple-effects on degraded soil improvement, so it is worthy of application and popularization.
基金Supported by the Special Fund for Agro-Scientific Research in the Public Interest of China(200903031)
文摘In view of Melilotus officinalis having high protein content and significant effect on improving saline alkali soil,a comprehensive experiment about improving saline alkali soil by planting M.officinalis was carried out to study its effects of potential equivalent land and equivalent grain.The results showed that the forage yield was 80 000 kg/hm^2 in the degraded grassland field( average pH of 9.06) after planting M.officinalis.The forage had a crude protein content of 17.50%,so the total content of crude protein was 1 400.64 kg/hm^2,which was equal to 15.562 t of corns produced from 3.557 hm^2 of medium-quality arable lands.Similarly,it was equal to 3.573 t of soybeans produced in 1.786 hm^2 of medium-quality arable lands.Therefore,based on such principle,the comprehensive development of creatures-soil system can acquire the equivalent land and equivalent grain resources.
