Hyperhomocysteinemia and Increased Oxidative Stress Levels Are Associated with Impaired Membrane Fluidity of Red Blood Cells in Hypertensive and Normotensive Men: An Electron Spin Resonance Investigation

Hyperhomocysteinemia and oxidative stress may be strongly linked to hypertension, atherosclerosis and other cardiovascular diseases. The present study was performed to investigate possible relationships among plasma total homocysteine, plasma 8-iso-prostaglandin F2α (8-isoPG F2α: an index of oxidative stress), and membrane fluidity (a reciprocal value of membrane microviscosity) in hypertension. We measured the membrane fluidity of red blood cells (RBCs) in hypertensive and normotensive men using an electron spin resonance (ESR) and spin-labeling method. Membrane fluidity of RBCs was significantly decreased in hypertensive men compared with normotensive men. Plasma total homocysteine levels were significantly higher in hypertensive men than in normotensive men, and correlated with plasma 8-isoPG F2α. In contrast, plasma nitric oxide (NO)-metabolites (an index of endothelial function) were lower in hypertensive men than in normotensive men. The reduced membrane fluidity of RBCs was associated with increased total homocysteine and plasma 8-isoPG F2α levels and decreased plasma NO-metabolite levels. Multivariate regression analysis showed that, after adjusting for general risk factors, plasma total homocysteine and 8-isoPG F2α were significant determinants of membrane fluidity of RBCs, respectively. These results suggest that hyperhomocysteinemia and oxidative stress with endothelial dysfunction might have a close correlation with impaired rheologic behavior of RBCs and circulatory disorders in hypertensive men.

Large-Scale Surface Modification of Decellularized Matrix with Erythrocyte Membrane for Promoting In Situ Regeneration of Heart Valve

In situ regeneration is a promising strategy for constructing tissue engineering heart valves(TEHVs).Currently,the decellularized heart valve(DHV)is extensively employed as a TEHV scaffold.Nevertheless,DHV exhibits limited blood compatibility and notable difficulties in endothelialization,resulting in thrombosis and graft failure.The red blood cell membrane(RBCM)exhibits excellent biocompatibility and prolonged circulation stability and is extensively applied in the camouflage of nanoparticles for drug delivery;however,there is no report on its application for large-scale modification of decellularized extracellular matrix(ECM).For the first time,we utilized a layer-by-layer assembling strategy to immobilize RBCM on the surface of DHV and construct an innovative TEHV scaffold.Our findings demonstrated that the scaffold significantly improved the hemocompatibility of DHV by effectively preventing plasma protein adsorption,activated platelet adhesion,and erythrocyte aggregation,and induced macrophage polarization toward the M2 phenotype in vitro.Moreover,RBCM modification significantly enhanced the mechanical properties and enzymatic stability of DHV.The rat models of subcutaneous embedding and abdominal aorta implantation showed that the scaffold regulated the polarization of macrophages into the anti-inflammatory and pro-modeling M2 phenotype and promoted endothelialization and ECM remodeling in the early stage without thrombosis and calcification.The novel TEHV exhibits excellent performance and can overcome the limitations of commonly used clinical prostheses.

Modeling biomembranes and red blood cells by coarse-grained particle methods

In this work, the previously developed coarse-grained （CG） particle models for biomembranes and red blood cells （RBCs） are reviewed, and the advantages of the CG particle methods over the continuum and atomistic simulations for modeling biological phenomena are discussed. CG particle models can largely increase the length scale and time scale of atomistic simulations by eliminating the fast degrees of freedom while preserving the mesoscopic structures and properties of the simulated system. Moreover, CG particle models can be used to capture the microstructural alternations in diseased RBCs and simulate the topological changes of biomembranes and RBCs, which are the major challenges to the typical continuum representations of membranes and RBCs. The power and versatility of CG particle methods are demonstrated：through simulating the dynamical processes mvolving significant topological .changes e.g. lipid self-assembly vesicle fusion and membrane budding.

Effect of ultraviolet blood irradiation and oxygenation on nerve function and function of the red blood cell membrane pump in patients with acute cerebral infarction

BACKGROUND： Ultraviolet blood irradiation and oxygenation （UBIO） has obtained better clinical effect in treating acute cerebral infarction, but the mechanism underlying this effect remains unclear. OBJECTIVE： To observe the effect of UBIO on the nerve function and activities of K^＋-Na^＋-ATPase and Ca2^＋-Mg2^＋-ATPase activities on the red blood cell （RBC） membrane of patients with acute cerebral infarction. DESIGN： A randomized and controlled study.SETTING： Department of Neurology, Xiangfan Central Hospital.PARTICIPANTS： From January 2000 to December 2001, excluding those above 70 years old, 58 cases of 700 patients with acute cerebral infarction admitted in the Department of Neurology, Xiangfan Central Hospital, were recruited and divided into two groups according to the random number table： UBIO treated group （n=28）, including 17 males and 11 females, aged 40-68 years; and control group （n=30）, including 20 males and 10 females, aged 44-69 years. All the patients agreed to participate in the therapeutic program and detected items. The general informations were comparable without obvious differences between the two groups （P 〉 0.05）.METHODS： ① The patients in both groups received routine treatments, besides, those in the UBIO treated group were given UBIO treatment by using the XL-200 type therapeutic apparatus produced in Shijiazhuang, whose ultraviolet wave was set at 253.7 nm with the energy density of 0.568 J/m^2 per second, UBIO treatment started from the second day after admission, once every other day, with a single course consisting of 5-7 treatments. ② In the UBIO treated group, the venous blood was sampled before and after the first, third and the completion of the treatment course respectively, the venous blood was taken at each corresponding time point in the control group. After centrifugation of the blood at 10 000 rounds per minute, the RBC membrane was separated and then the activities of K^＋-Na^＋-ATPase and Ca2^＋-Mg2^＋-ATPase were detected by means of phosphorus determination.③ The nerve function was scored before and after treatment in both groups with European stroke scale, which included 13 items, the total score was 0-100 points, the higher the score, the better the nerve function. MAIN OUTCOME MEASURES ：①Score of European stroke scale before and after treatment in both groups.② Comparison of the activities of K^＋-Na^＋-ATPase and Ca2^＋-Mg2^＋-ATPase on RBC membrane between the two groups before treatment and after the first, third and the completion of the treatment. RESULTS： All the 58 patients with cerebral infarction were involved in the analysis of results.① The score of European stroke scale had no obvious difference between the two groups [（49.31±11.48）, （50.58±12.63）, P 〉 0.05], and it was obviously higher in the UBIO treated group than in the control group after treatment [84.66±13.75）, （77.05±11.17）, P 〈 0.05].②The activity of K^＋-Na^＋-ATPase on RBC membrane in the UBIO treated group was significantly increased after the first and third treatment as compared with before treatment [（31.56±19.25）, （27.64±15.83）, （17.67±13.83）, P 〈 0.01], it was still higher after the completion of the treatment than before treatment without obvious difference [（20.86±14.53）, P 〉 0.05]. After the first and third treatment, it was obviously higher in the UBIO treated group than in the control group [19.31±11.88）, （17.44±10.42）, P 〈 0.01]. ③ In the UBIO treated group, Ca2^＋-Mg2^＋-ATPase activity on RBC membrane significantly increased after the first treatment and remained higher than the pre-treatment level throughout the treatment [（27.49±14.72）, （17.41±4.82）, P 〈 0.01]. The activity of Ca2^＋-Mg2^＋-ATPase on RBC membrane was markedly higher in the UBIO treated group than in the control group after after the first, third and the completion of treatment respectively [（24.83±12.88）, （17.70±5.69）; （28.08±13.44）, （16.32±5.29）; （17.42±6.04）, P〈 0.05-0.01]. CONCLUSION： The effect of UBIO treatment against acute cerebral infarction may be mediated by the increased K^＋-Na^＋ ATPase and Ca2^＋-Mg2^＋-ATPase activities on RBC membrane, which enhances the RBC transformation ability so as to lower RBC aggregation and correct high blood viscosity.

Analysis of Mechanical Behavior of Red Blood Cell Membrane with Malaria Infection

Human red blood cells (RBCs) are responsible to transport oxygen and carbon dioxide for human bodies. The physiological functions of RBCs are greatly influenced by their mechanical properties. When RBC is infected by Malaria parasite called Plasmodium falciparum, it shows progressive changes in mechanical properties and loses its deformability. The infected red blood cells (IRBCs) develop properties of cytoadherence (stickiness) and rosetting (the binding of non-infected RBCs to parasitized RBCs). In this paper to analyze the mechanical properties and deformability of the IRBC, we applied stress-stretch ratio relation of its biomembrane .To express this constitutive relation, we proposed a mathematical model (Neo-Hookean model) based on membrane theory. On this model, we present continuous stress-stretch ratio curves for the relation derived from the model for different intracellular developmental stages of the parasite, to determine the mechanical properties of IRBC. The analytical results obtained from the mathematical model are more closed with the experimental data [1] which demonstrates the validity of the model. By restricting our attention to spherically symmetric deformation in the final schizont stage of parasite development, the pressure-extension ratio relation curve also adapted from the proposed strain energy function. The change in osmotic pressure versus volumetric ratio has been also considered for IRBC before hemolysis.

Red blood cell membrane-coated FLT3 inhibitor nanoparticles to enhance FLT3-ITD acute myeloid leukemia treatment

FMS-like tyrosine kinase 3(FLT3)is a viable and important therapeutic target for acute myeloid leukemia(AML).FLT3 internal tandem duplication(FLT3-ITD)mutations have been identified in approximately 30%of AML patients,and are associated with unfavorable prognosis,higher risk of relapse,drug resistance,and poor clinical outcome.Even FLT3 inhibitors have demonstrated promising efficacy,they cannot cure AML or even significantly extend the lives of patients with FLT3-ITD mutations.This is partly because of poor water solubility,insufficient membrane penetration and short half-life of small molecule inhibitors.Besides,the presence of enzymes like CYP3A4 in bone marrow accelerate the elimination and metabolism of FLT3 inhibitors,resulting in low plasma concentrations and side effects.Here we report the erythrocyte membrane-camouflaged FLT3 inhibitor nanoparticles to enhance FLT3-ITD AML treatment.Briefly,we physically coextruded red blood cell(RBC)membrane vesicles with nanoparticles derived from FLT3 inhibitor F30 to obtain F30@RBC-M,which exhibited comparable potent FLT3-ITD inhibitory effects compared to free F30 in vitro,while displaying a higher potent antitumor efficacy in xenograft models due to the prolonged circulation properties.Furthermore,administration of F30@RBC-M significantly extended the survival of mice in a transplanted mouse model than F30 free drug.These findings suggest that RBC membrane-coated nanoparticles derived from FLT3 inhibitors hold promise as a tool to enhance the therapeutic efficacy to treat FLT3-ITD AML.

RBC Membrane Camouflaged Semiconducting Polymer Nanoparticles for Near-Infrared Photoacoustic Imaging and Photothermal Therapy

Semiconducting conjugated polymer nanoparticles(SPNs)represent an emerging class of phototheranostic materi-als with great promise for cancer treatment.In this report,low-bandgap electron donoracceptor(DA)-conjugated SPNs with sur-face cloaked by red blood cell membrane(RBCM)are developed for highly e ective photoacoustic imaging and photothermal therapy.The resulting RBCM-coated SPN(SPN@RBCM)displays remarkable near-infrared light absorption and good photosta-bility,as well as high photothermal conver-sion e ciency for photoacoustic imaging and photothermal therapy.Particularly,due to the small size(<5 nm),SPN@RBCM has the advantages of deep tumor penetration and rapid clearance from the body with no appreciable toxicity.The RBCM endows the SPNs with prolonged systematic circulation time,less reticuloendothelial system uptake and reduced immune-recognition,hence improving tumor accumulation after intravenous injection,which provides strong photoacoustic signals and exerts excellent photothermal therapeutic e ects.Thus,this work provides a valuable paradigm for safe and highly e cient tumor pho-toacoustic imaging and photothermal therapy for further clinical translation.

Adenovirus-mediated expression of pig α(1,3) galactosyltransferase reconstructs Gal α(1,3) Gal epitope on the surface of human tumor cells

Gal alpha(1, 3) Gal (gal epitope) is a carbohydrate epitope and synthesized in large amount by alpha(1, 3) galactosyltransferase [alpha(1, 3) GT] enzyme on the cells of lower mammalian animals such as pigs and mice. Human has no gal epitope due to the inactivation of alpha(1, 3) GT gene but produces a large amount of antibodies (anti-Gal) which recognize Gal alpha(1, 3) Gal structures specifically. In this study, a replication-deficient recombinant adenoviral vector Ad5sGT containing pig alpha(1, 3) GT cDNA was constructed and characterized. Adenoviral vector-mediated transfer of pig alpha(1, 3) GT gene into human tumor cells such as malignant melanoma A375, stomach cancer SGC-7901, and lung cancer SPC-A-1 was reported for the first time. Results showed that Gal epitope did not increase the sensitivity of human tumor cells to human complement-mediated lysis, although human complement activation and the binding of human IgG and IgM natural antibodies to human tumor cells were enhanced significantly after Ad5sGT transduction. Appearance of gal epitope on the human tumor cells changed the expression of cell surface carbohydrates reacting with Ulex europaeus I (UEA I) lectins, Vicia villosa agglutinin (VVA), Arachis hypogaea agglutinin (PNA), and Glycine max agglutinin (SBA) to different degrees. In addition, no effect of gal epitope on the growth in vitro of human tumor cells was observed in MTT assay.

补阳还五汤对高脂血症模型小鼠红细胞的影响

目的研究补阳还五汤对高脂血症模型小鼠红细胞的影响。方法以高脂饲料喂养雄性C57BL/6小鼠制备高脂血症模型。将造模成功的小鼠随机分为模型组、阿托伐他汀钙组(0.26 g/kg)、非诺贝特组(1.3 mg/kg)和补阳还五汤高、中、低剂量组(18.6、9.3、4.6 g/kg),每组10只;另取10只以普通饲料喂养的小鼠作为正常组。各组小鼠灌胃相应药物或生理盐水,每日1次,连续21 d。末次给药后,各组小鼠称体重,检测血糖、总胆固醇(TC)、甘油三酯(TAG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平,观察红细胞形态变化,检测红细胞膜中葡萄糖转运蛋白1(GLUT1)、红细胞膜蛋白带4.2(protein 4.2)、小窝蛋白1(caveolin-1)、筏蛋白1(flotillin-1)的表达水平。结果与模型组比较,补阳还五汤各剂量组小鼠体重和TC、TAG、HDL-C、LDL-C水平以及GLUT1、caveolin-1、flotillin-1蛋白表达水平均显著降低,血糖水平和protein 4.2蛋白表达水平均显著升高(P<0.05);补阳还五汤低、中剂量组小鼠红细胞形态明显改善。结论补阳还五汤可降低高脂血症模型小鼠的血脂水平,改善高脂环境中红细胞的形态及功能,有预防高脂血症并发症动脉粥样硬化的潜力。

红细胞-纳米载体递药系统研究进展

脂质体、聚合物纳米粒、聚合物胶束等纳米载体是近年来药物递送的研究热点,但其给药后易被人体免疫系统识别和清除。细胞搭载技术能延长纳米载体在体内的循环,红细胞作为内源性细胞,具有来源丰富、高生物相容性和生物消除机制安全等优点,从而推动了红细胞-纳米载体递药系统的发展。基于此,本文综述了红细胞-纳米载体递药系统的研究进展,总结了基于红细胞与纳米载体的构建方式不同的几种红细胞-纳米载体递药系统的研究进展。

Effect on Life Span and Membrane Protein in Red Blood Cells by Integrated Medicine Therapy on Chronic Aplastic Anemia

Objective: To explore the mechanism ofintegrated traditional Chinese and Westernmedicine (TCM--WM ) therapy on chronicaplastic anemia (CAA). Methods: The RBClife span of 30 normal human subjects and 30patients with CAA were measured by sir labelled technique before and after TCM--WMtherapy. The morphology and distribution ofRBC membrane protein granules were observed by freeze fracture etching and transmission electron microscope. Results: The halflife of erythrocytes (RBC TI/2)was shortenedin CAA cases and there was a significant difference compared to healthy control (P <0. 01). After therapy, the RBC life span prolonged and approached the normal level. Before treatment, there existed abnormal in morphology, decrease in amount and uneven indistribution of protein granules in protoplasmicface (PF) and extracellular face (EF) of RBCmembrane. After treatment, the protein granules of RBC membrane was improved and approached to control. Conclusions: The morphology, amount, quality and distribution ofRBC membrane protein granule were closelyrelated to its life span. The therapeutic effectof TCM--WM was better than that of WMalone and it had a function both in stabilizingmembrane protein and extending the RBC lifespan.

犬红细胞膜仿生纳米载体的制备和体外毒性及靶向初探

为了提高纳米颗粒的安全性和靶向性,试验采用低渗处理法制备犬红细胞膜(RBCM),双乳法制备聚乳酸-羟基乙酸共聚物纳米颗粒(BLANK-PLGA),并用脂质体挤出器挤出法制备犬红细胞膜囊泡(RBCM-NVE)和犬红细胞膜仿生纳米载体(PLGA-RBCM);采用透射电镜、动态光散射技术对不同纳米颗粒的结构、大小和电位进行表征鉴定;通过稳定性试验考察不同纳米颗粒的粒径及聚集性变化;采用SDS-PAGE和Western-blot法验证挤出前后细胞CD47膜蛋白表达情况;通过犬乳腺肿瘤细胞(CIPp)摄取BLANK-PLGA和PLGA-RBCM研究纳米颗粒的同源靶向性;通过体外试验探究BLANK-PLGA和RBCM-NVE纳米颗粒的细胞毒性。结果表明:BLANK-PLGA呈球形结构,RBCM-NVE呈杯状囊泡结构,PLGA-RBCM呈明显的“核-壳”结构;BLANK-PLGA平均粒径为(305.17±2.59)nm,聚合物分散性指数(polymer dispersity index, PDI)为0.10±0.01,平均电位为(-3.86±0.59)mV;RBCM-NVE平均粒径为(200.50±0.26)nm, PDI为0.22±0.02,平均电位为(-9.60±0.88)mV;PLGA-RBCM平均粒径为(274.16±2.11)nm, PDI为0.18±0.03,平均电位为(-6.03±0.64)mV;第0~25天,在PBS中PLGA-RBCM粒径保持在300 nm以下,PDI为0.2左右,随着时间推移,BLANK-PLGA的粒径与PDI均明显增大,粒径大小不均;BLANK-PLGA和PLGA-RBCM在胎牛血清中4 h内吸光度没有较大变化;RBCM在通过脂质体挤出器挤出前后均保持相同的蛋白条带并且均表达CD47膜蛋白;CIPp细胞对PLGA-RBCM的摄取量极显著多于对BLANK-PLGA(P<0.01);BLANK-PLGA和RBCM-NVE与CIPp细胞共孵育6,12,24 h后细胞活力差异不显著(P>0.05)。说明成功制备的PLGA-RBCM具有良好的稳定性、安全性及对同源细胞的靶向性。

生长抑素联合低分子肝素对高脂血症性AP患者血液高凝状态、PCT、CRP及RAAS的调节作用

目的探讨生长抑素联合低分子肝素对高脂血症性急性胰腺炎(HLAP)患者血液高凝状态、降钙素原(PCT)、C反应蛋白(CRP)及肾素-血管紧张素-醛固酮系统(RAAS)的影响。方法以平顶山市第二人民医院宝丰分院2020年1月至2023年1月收治的66例HLAP患者为研究对象,根据治疗方案将患者分为参照组(33例)和联合组(33例)。参照组采用生长抑素治疗,联合组采用生长抑素联合低分子肝素治疗。比较两组临床疗效、临床症状改善时间、治疗前后血液流变学指标(红细胞聚集指数、血浆黏度、全血低切黏度、全血高切黏度)、血清RAAS指标[血管紧张素-Ⅱ(Ang-Ⅱ)、肾素(E)、醛固酮(ALD)]、血清PCT、CRP、热休克蛋白70(HSP70)、热休克蛋白27(HSP27)水平。结果联合组临床总有效率(96.97%)高于参照组(75.76%),差异有统计学意义(P<0.05);联合组肠道功能恢复时间、血淀粉酶恢复正常时间、腹膜炎体征及腹痛消失时间短于参照组,差异均有统计学意义(P<0.05);治疗后联合组红细胞聚集指数、全血低切黏度、全血高切黏度及血浆黏度降低幅度大于参照组,差异均有统计学意义(P<0.05);治疗后联合组血清E、Ang-Ⅱ、ALD水平低于参照组,差异均有统计学意义(P<0.05);与治疗前相比,各组治疗后血清PCT、CRP、HSP70、HSP27水平明显降低,其中联合组降低幅度更为显著,差异均有统计学意义(P<0.05)。结论生长抑素联合低分子肝素治疗HLAP疗效显著,能够改善血液高凝状态,减轻炎症反应,有利于患者病情恢复。

联合检测C反应蛋白/纤维蛋白原、总胆红素、红细胞分布宽度预测慢性阻塞性肺疾病急性加重患者治疗应答的效能

目的分析联合检测C反应蛋白(CRP)与纤维蛋白原(FIB)比值、总胆红素、红细胞分布宽度(RDW)预测慢性阻塞性肺疾病急性加重(AECOPD)患者治疗应答的效能。方法选取2020年1月至2023年2月收治的265例AECOPD患者,根据疗效分为有效组241例和无效组24例,比较2组治疗前、治疗3 d后、治疗5 d后CRP/FIB、总胆红素、RDW水平,分析治疗5 d后CRP/FIB、总胆红素、RDW水平对疗效的影响,受试者工作特征曲线分析治疗3、5 d后CRP/FIB、总胆红素、RDW及联合对AECOPD疗效预测效能。结果有效组治疗3、5 d后CRP/FIB、RDW低于无效组,总胆红素高于无效组(P<0.05)。危险度分析显示,治疗5 d后CRP/FIB、RDW高表达亚组无效的风险分别是低表达亚组的1.081、1.099倍(P<0.05);总胆红素高表达亚组无效的风险是低表达亚组的0.922倍(P<0.05);绘制受试者工作特征曲线显示,治疗3 d后CRP/FIB、总胆红素联合RDW的曲线下面积大于各指标单独的曲线下面积(P<0.01);治疗5 d后CRP/FIB、总胆红素联合RDW的曲线下面积大于各指标单独的曲线下面积(P<0.01);治疗5 d后CRP/FIB、总胆红素联合RDW的曲线下面积最大,为0.932,其预测敏感度为95.83%,特异度为84.23%。结论CRP/FIB、RDW、总胆红素的变化与AECOPD治疗应答有关,均可作为预测治疗应答的标志物,联合检测三者能进一步提高预测能力,为疗效的早期预测、治疗、病情分层等提供参考。

参芎葡萄糖治疗慢性阻塞性肺疾病合并肺源性心脏病的临床效果

目的探讨参芎葡萄糖治疗慢性阻塞性肺疾病(COPD)合并肺源性心脏病(PHD)的临床效果。方法选取2022年1月—2024年1月收治的228例COPD合并PHD,采用随机数字表法随机将其分为观察组和对照组2组各114例。观察组给予参芎葡萄糖联合常规治疗,对照组给予常规治疗。比较2组血常规指标(红细胞、血红蛋白、红细胞比容、血小板计数)、凝血指标[D-二聚体(D-D)、凝血酶原时间(PT)、活化部分凝血酶原时间(APTT)、纤维蛋白原(FIB)]、肝肾功能[血尿素(BUN)、血肌酐(Scr)、天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、总蛋白(TP)]、血气指标[动脉血氧分压(PaO_(2))、动脉血二氧化碳分压(PaCO_(2))、动脉血氧饱和度(SaO_(2))]、心型脂肪酸结合蛋白(H-FABP)、肌钙蛋白I(cTnI)、N末端B型脑钠肽前体(NT-proBNP)、肺动脉收缩压(PASP)、急性生理学及慢性健康状况评估系统Ⅱ(APACHEⅡ)评分,并统计入院28 d病死情况、治疗期间不良反应。结果治疗后,2组红细胞、血红蛋白、红细胞比容、血小板计数、D-D、FIB、PaCO_(2)、H-FABP、cTnI、NT-proBNP、PASP、APACHEⅡ低于治疗前,且观察组低于对照组;2组PT、APTT、PaO_(2)、SaO_(2)高于治疗前,且观察组高于对照组(P<0.05,P<0.01)。治疗前后,2组组间和组内BUN、Scr、AST、ALT、TP比较无明显差异(P>0.05)。观察组入院28 d病死率和治疗期间不良反应发生率(7.02%,8/114;7.89%,9/114)与对照组(10.53%,12/114;5.26%,6/114)比较无明显差异(P>0.05)。结论参芎葡萄糖可改善COPD合并PHD患者血常规、凝血指标及血气指标,并降低H-FABP、cTnI、NT-proBNP水平,进而减轻心肌损伤程度,缓解患者病情。

Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats

Litsea elliptica Blume leaves have been traditionally used as medicinal herbs because of its antimutagenicity,chemopreventative and insecticidal properties. In this study,the toxic effects of L. elliptica essential oil against Sprague-Dawley rat’s red blood cells (RBCs) were evaluated. L. elliptica essential oil was given by oral gavage 5 times per week for 3 treated groups in the doses of 125,250,and 500 mg/(kg body weight),respectively,and the control group received distilled water. Full blood count,RBC osmotic fragility,RBC morphological changes,and RBC membrane lipid were analyzed 28 d after the treatment. Although L. elliptica essential oil administration had significantly different effects on hemoglobin (Hb),mean cell hemoglobin concentration (MCHC),mean cell volume (MCV),and mean cell hemoglobin (MCH) in the experimental groups as compared to the control group (P<0.05),the values were still within the normal range. L. elliptica induced morphological changes of RBC into the form of echinocyte. The percentage of echinocyte increased significantly among the treated groups in a dose-response manner (P<0.001). The concentrations of RBC membrane phospholipids and cholesterol of all treated groups were significantly lower than those of control group (P<0.001). However,the RBC membrane osmotic fragility and total proteins of RBC membrane findings did not differ significantly between control and treated groups (P>0.05). It is concluded that structural changes in the RBC membrane due to L. elliptica essential oil administration did not cause severe membrane damage.

Protective Effect of <i>Aloe vera (Aloe barbadensis</i>Miller) on Erythrocytes Anion Transporter and Oxidative Change

The purpose of this study was to evaluate the ability of aqueous extract of Aloe barbadensis Miller (Aloe vera) on oxidative damage and Anion Exchanger 1 (AE1, also known as Band 3) expression in human erythrocytes exposed to the water soluble free radical initiator 2.2’-azobis-2-amidinopropano dihydrochloride (AAPH). In addition, total phenolic compounds in the extracts were determined as catechin equivalent and the various antioxidant activities were compared to natural and synthetic standard antioxidants such as BHA and ascorbic acid. Since Aloe vera extract did not cause a consumption of the cytosolic antioxidant, glutathione (GSH) when it was direct incubated with GSH in basic aerated aqueous solution, this indicates that Aloe vera extract does not proceed auto oxidation at this experimental condition. Furthermore, Aloe vera extract prevent the consumption of GSH, in radical treated RBCs. It also inhibit consumption of GSH when it was direct incubated with AAPH. Aloe vera gel extract inhibits the generation of diphenyl-2-picrylhy-drazyl (DPPH) and the scavenging activity was increased in a dose dependent manner. Aloe vera extract was shown the similar reducing power than standards BHT and ascorbic acid. Biochemical analysis by SDS-PAGE and western blotting showed that AAPH-induced oxidative stress increased the susceptibility of AE1 to proteolytic degradation. Of note, our data evidenced that Aloe vera treatment was able to partially restore the normal RBC membrane protein profiles in a dose-dependent manner. These results clearly demonstrate the antioxidative activity of Aloe vera gel extract that might be ascribed to a synergistic action of the bioactive compounds contained therein.

母血及羊水中TIM-3、GATA-3水平对胎膜早破合并宫内感染的诊断价值

目的分析母血及羊水中T细胞免疫球蛋白黏蛋白分子-3(TIM-3)、GATA结合蛋白3(GATA-3)水平对胎膜早破(PROM)合并宫内感染的诊断价值。方法选择PROM患者89例作为研究对象,发生宫内感染者42例作为感染组,未发生宫内感染者47例作为未感染组,并选择同期正常足月孕妇45例作为对照组。根据宫内感染严重程度将PROM合并宫内感染患者分成轻度组16例、中度组17例、重度组9例。检测血、羊水中TIM-3、GATA-3mRNA及蛋白水平,比较对照组、未感染组、感染组和不同感染程度孕妇血、羊水中TIM-3、GATA-3 mRNA及蛋白水平,受试者工作特征曲线分析血清及羊水中TIM-3、GATA-3蛋白单独及联合诊断PROM孕妇发生宫内感染的效能。结果对照组、未感染组、感染组血及羊水中TIM-3 mRNA、蛋白水平依次升高(P<0.05),GATA-3 m RNA、蛋白水平依次降低(P<0.05);轻度组、中度组、重度组血及羊水中TIM-3 mRNA、蛋白水平依次升高(P<0.05),GATA-3mRNA、蛋白水平依次降低(P<0.05);血清TIM-3、GATA-3蛋白单独及联合,羊水TIM-3、GATA-3蛋白单独及联合诊断PROM孕妇发生宫内感染的曲线下面积分别为0.854、0.813、0.937,0.834、0.850、0.922。结论PROM合并宫内感染患者母血及羊水中TIM-3呈高表达,GATA-3呈低表达,两者均对PROM孕妇发生宫内感染有一定的诊断价值,联合诊断效能更高。

早期营养管理对急性胰腺炎患者预后及TBIL、CRP、RDW的影响

目的 探究早期营养管理对急性胰腺炎患者预后及总胆红素(TBIL)、C反应蛋白(CRP)、红细胞分布宽度(RDW)的影响。方法 选取2021年8月至2022年3月北京积水潭医院的急性胰腺炎患者129例为研究对象,采用随机数字表法分为对照组(n=64)与观察组(n=65),对照组患者给予常规护理,观察组患者在常规护理的基础上予以早期营养管理,两组均护理至出院日。记录两组肠功能恢复时间,比较两组干预前后Ranson评分、TBIL、CRP、RDW及预后情况。结果 观察组肠鸣音消失时间、排气和腹胀恢复所需时间均短于对照组,差异均具有统计学意义(t=10.866、2.834、5.759,P<0.05)。干预前,两组Ranson评分、TBIL、CRP、RDW水平比较,差异无统计学意义(t=0.974、0.305、0.808、0.212,P>0.05);干预后,两组Ranson评分、TBIL、CRP、RDW水平均降低,且观察组低于对照组,差异均具有统计学意义(t=3.819、2.069、9.170、2.094,P<0.05)。观察组并发症发生率6.15%(4/65)显著高于对照组18.75%(12/64),差异有统计学意义(χ^(2)=4.709,P<0.05)。结论 早期营养管理可缩短急性胰腺炎患者肠功能恢复时间,改善TBIL、CRP、RDW水平及预后。

血清RDW、H-FABP在房颤合并急性心肌梗死患者中的表达水平及临床意义

目的分析血清红细胞分布宽度(RDW)、心型脂肪酸结合蛋白(H-FABP)在房颤合并急性心肌梗死患者中的表达水平及临床意义。方法选取2016年8月至2021年8月我院收治的60例房颤患者,根据其是否发生急性心肌梗死分为心肌梗死组(n=18)和非心肌梗死组(n=42)。比较两组患者的血清RDW、H-FABP水平,分析血清RDW、H-FABP水平与房颤合并急性心肌梗死发生的相关性,并评价其用于诊断房颤合并急性心肌梗死的价值。结果心肌梗死组的血清RDW、H-FABP水平均高于非心肌梗死组(P<0.05)。Spearman分析显示血清RDW、H-FABP水平与房颤合并急性心肌梗死的发生呈正相关(P<0.05)。ROC曲线分析显示血清RDW、H-FABP水平诊断房颤合并急性心肌梗死的曲线下面积分别为0.641(95%CI:0.364~0.917)、0.750(95%CI:0.504~0.997)。结论血清RDW、H-FABP在房颤合并急性心肌梗死患者中呈现高表达,其水平与房颤患者急性心肌梗死的发生存在相关性,可有效预测房颤患者急性心肌梗死的发生。