Objective Alzheimer’s disease(AD)has become a significant global concern,but effective drugs able to slow down AD progression is still lacked.Electroacupuncture(EA)has been demonstrated to ameliorate cognitive impair...Objective Alzheimer’s disease(AD)has become a significant global concern,but effective drugs able to slow down AD progression is still lacked.Electroacupuncture(EA)has been demonstrated to ameliorate cognitive impairment in individuals with AD.However,the underlying mechanisms remains poorly understood.This study aimed at examining the neuroprotective properties of EA and its potential mechanism of action against AD.Methods APP/PS1 transgenic mice were employed to evaluate the protective effects of EA on Shenshu(BL 23)and Baihui(GV 20).Chemogenetic manipulation was used to activate or inhibit serotonergic neurons within the dorsal raphe nucleus(DRN).Learning and memory abilities were assessed by the novel object recognition and Morris water maze tests.Golgi staining,western blot,and immunostaining were utilized to determine EA-induced neuroprotection.Results EA at Shenshu(BL 23)and Baihui(GV 20)effectively ameliorated learning and memory impairments in APP/PS1 mice.EA attenuated dendritic spine loss,increased the expression levels of PSD95,synaptophysin,and brain-derived neurotrophic factor in hippocampus.Activation of serotonergic neurons within the DRN can ameliorate cognitive deficits in AD by activating glutamatergic neurons mediated by 5-HT1B.Chemogenetic inhibition of serotonergic neurons in the DRN reversed the effects of EA on synaptic plasticity and memory.Conclusion EA can alleviate cognitive dysfunction in APP/PS1 mice by activating serotonergic neurons in the DRN.Further study is necessary to better understand how the serotonergic neurons-related neural circuits involves in EA-induced memory improvement in AD.展开更多
To evaluate the operation comfortability in the master-slave robotic minimally invasive surgery(MIS), an optimal function was built with two operation comfortability decided indices, i.e., the center distance and volu...To evaluate the operation comfortability in the master-slave robotic minimally invasive surgery(MIS), an optimal function was built with two operation comfortability decided indices, i.e., the center distance and volume contact ratio. Two verifying experiments on Phantom Desktop and Micro Hand S were conducted. Experimental results show that the operation effect at the optimal relative location is better than that at the random location, which means that the optimal function constructed in this paper is effective in optimizing the operation comfortability.展开更多
目的观察郑氏手法治疗肱骨外上髁炎的治疗效果。方法将2021年8月—2022年1月该院筋伤科门诊收治的60例肱骨外上髁炎病人按照纳入顺序用随机数字表随机分类法随机分成两组,每组30例,治疗组30例病人予郑氏手法治疗,对照组30例病人予局部...目的观察郑氏手法治疗肱骨外上髁炎的治疗效果。方法将2021年8月—2022年1月该院筋伤科门诊收治的60例肱骨外上髁炎病人按照纳入顺序用随机数字表随机分类法随机分成两组,每组30例,治疗组30例病人予郑氏手法治疗,对照组30例病人予局部封闭治疗,两组疗程均是2周。疗程结束后评估两组总体疗效,治疗前后视觉模拟评分法(visual analog scale,VAS)评分,治疗前后Mayo肘关节功能评分,治疗前后美国特种外科医院(Hospital for Special Surgery,HSS)肘关节功能评分,治疗前后肘关节局部压痛阈值。结果治疗组的总有效率为88.89%(24/27),优于对照组总有效率81.48%(22/27)(P<0.05);两组治疗后VAS评分均降低,两组差异无统计学意义(P>0.05);两组治疗后Mayo肘关节评分和HSS肘关节评分均增高,治疗组评分明显高于封闭组(P<0.05);两组治疗后Mayo肘关节评分和HSS肘关节评分均增高,治疗组评分明显高于封闭组(P<0.05);两组治疗后肘关节局部压痛阈值较治疗前均有提升,治疗组肘关节局部压痛阈值明显高于封闭组(P<0.05)。结论郑氏手法治疗肱骨外上髁炎疗效优于局部封闭治疗,值得临床推广应用。展开更多
基金supported by grants from the Shenzhen Science and Technology Program(No.2021-22154)National Natural Science Foundation of China(No.82205271,No.82374564,and No.82074566)+1 种基金Wuhan Medical Research Project(No.WZ21Q09)Key Chinese Medicine Project of Hubei Provincial Natural Science Foundation(No.2023AFD112).
文摘Objective Alzheimer’s disease(AD)has become a significant global concern,but effective drugs able to slow down AD progression is still lacked.Electroacupuncture(EA)has been demonstrated to ameliorate cognitive impairment in individuals with AD.However,the underlying mechanisms remains poorly understood.This study aimed at examining the neuroprotective properties of EA and its potential mechanism of action against AD.Methods APP/PS1 transgenic mice were employed to evaluate the protective effects of EA on Shenshu(BL 23)and Baihui(GV 20).Chemogenetic manipulation was used to activate or inhibit serotonergic neurons within the dorsal raphe nucleus(DRN).Learning and memory abilities were assessed by the novel object recognition and Morris water maze tests.Golgi staining,western blot,and immunostaining were utilized to determine EA-induced neuroprotection.Results EA at Shenshu(BL 23)and Baihui(GV 20)effectively ameliorated learning and memory impairments in APP/PS1 mice.EA attenuated dendritic spine loss,increased the expression levels of PSD95,synaptophysin,and brain-derived neurotrophic factor in hippocampus.Activation of serotonergic neurons within the DRN can ameliorate cognitive deficits in AD by activating glutamatergic neurons mediated by 5-HT1B.Chemogenetic inhibition of serotonergic neurons in the DRN reversed the effects of EA on synaptic plasticity and memory.Conclusion EA can alleviate cognitive dysfunction in APP/PS1 mice by activating serotonergic neurons in the DRN.Further study is necessary to better understand how the serotonergic neurons-related neural circuits involves in EA-induced memory improvement in AD.
基金Supported by the National High Technology Research and Development Program of China("863" Program,No.2012AA02A606)the Research Project of State Key Laboratory of Mechanical System and Vibration(MSV201412)
文摘To evaluate the operation comfortability in the master-slave robotic minimally invasive surgery(MIS), an optimal function was built with two operation comfortability decided indices, i.e., the center distance and volume contact ratio. Two verifying experiments on Phantom Desktop and Micro Hand S were conducted. Experimental results show that the operation effect at the optimal relative location is better than that at the random location, which means that the optimal function constructed in this paper is effective in optimizing the operation comfortability.
文摘目的观察郑氏手法治疗肱骨外上髁炎的治疗效果。方法将2021年8月—2022年1月该院筋伤科门诊收治的60例肱骨外上髁炎病人按照纳入顺序用随机数字表随机分类法随机分成两组,每组30例,治疗组30例病人予郑氏手法治疗,对照组30例病人予局部封闭治疗,两组疗程均是2周。疗程结束后评估两组总体疗效,治疗前后视觉模拟评分法(visual analog scale,VAS)评分,治疗前后Mayo肘关节功能评分,治疗前后美国特种外科医院(Hospital for Special Surgery,HSS)肘关节功能评分,治疗前后肘关节局部压痛阈值。结果治疗组的总有效率为88.89%(24/27),优于对照组总有效率81.48%(22/27)(P<0.05);两组治疗后VAS评分均降低,两组差异无统计学意义(P>0.05);两组治疗后Mayo肘关节评分和HSS肘关节评分均增高,治疗组评分明显高于封闭组(P<0.05);两组治疗后Mayo肘关节评分和HSS肘关节评分均增高,治疗组评分明显高于封闭组(P<0.05);两组治疗后肘关节局部压痛阈值较治疗前均有提升,治疗组肘关节局部压痛阈值明显高于封闭组(P<0.05)。结论郑氏手法治疗肱骨外上髁炎疗效优于局部封闭治疗,值得临床推广应用。
