The effects of plant growth regulators on peppermint (Mentha piperita) cultured in vitro were studied for the purpose of maximizing growth and essential oil production in micropropagated plants. The basal medium was e...The effects of plant growth regulators on peppermint (Mentha piperita) cultured in vitro were studied for the purpose of maximizing growth and essential oil production in micropropagated plants. The basal medium was experimentally supplemented with the auxin 4-indol-3-ylbutyric acid (IBA) and the cytokinin 6-benzylaminopurine (BAP) individually and in combination. Supplementation with BAP alone resulted in the highest values for root length, root dry weight, shoot length, and numbers of nodes, leaves, and ramifications. Treatment with IBA alone or with IBA + BAP resulted in a ~50% increase in shoot fresh weight. The production of secondary metabolites was affected only by the addition of cytokinin, which resulted in a ~40% increase in the total yield of essential oils (EOs). Similar trends were observed for yields of the major EO components (menthone, menthol, pulegone, and menthofuran). Our findings demonstrate that the application of growth regulators increases EO production and biomass concomitantly in an herbaceous species rich in commercially valuable terpenes.展开更多
AIM:To examine the antiulcerogenic effects of various extracts of Mentha arvensis Linn on acid,ethanol and pylorus ligated ulcer models in rats and mice.METHODS:Various crude extracts of petroleum ether,chloroform,or ...AIM:To examine the antiulcerogenic effects of various extracts of Mentha arvensis Linn on acid,ethanol and pylorus ligated ulcer models in rats and mice.METHODS:Various crude extracts of petroleum ether,chloroform,or aqueous at a dose of 2 g/kg po did not produce any signs or symptoms of toxicity in treated animals.In the pyloric ligation model oral administration of different extracts such as petroleum ether,chloroform and aqueous at 375 mg/kg po,standard drug ranitidine 60 mg/kg po and control group 1 Tween 80,5 mL/kg po to separate groups of Wister rats of either sex(n = 6) was performed.Total acidity,ulcer number,scoring,incidence,area,and ulcer index were assessed.RESULTS:There was a decrease in gastric secretion and ulcer index among the treated groups i.e.petroleum ether(53.4),chloroform(59.2),aqueous(67.0) and in standard drug(68.7) when compared to the negative control.In the 0.6 mol/L HCl induced ulcer model in rats(n = 6) there was a reduction in ulcerative score in animals receiving petroleum ether(50.5),chloroform(57.4),aqueous(67.5) and standard.drug(71.2) when compared to the negative control.In the case of the 90 ethanol-induced ulceration model(n = 6) in mice,there was a decrease in ulcer score in test groups of petroleum ether(53.11),chloroform(62.9),aqueous(65.4) and standard drug ranitidine(69.7) when compared to the negative control.It was found that pre-treatment with various extracts of Mentha arvensis Linn in three rat/mice ulcer models ie ibuprofen plus pyloric ligation,0.6 mol/L HCl and 90 ethanol produced significant action against acid secretion(49.3 ± 0.49 vs 12.0 ± 0.57,P < 0.001).Pre-treatment with various extracts of Mentha arvensis Linn showed highly-significant activity against gastric ulcers(37.1 ± 0.87 vs 12.0 ± 0.57,P < 0.001).CONCLUSION:Various extracts of Mentha arvensis Linn.375 mg/kg body weight clearly shows a protective effect against acid secretion and gastric ulcers in ibuprofen plus pyloric ligation,0.6 mol/L HCl induced and 90 ethanol-induced ulcer models.展开更多
文摘The effects of plant growth regulators on peppermint (Mentha piperita) cultured in vitro were studied for the purpose of maximizing growth and essential oil production in micropropagated plants. The basal medium was experimentally supplemented with the auxin 4-indol-3-ylbutyric acid (IBA) and the cytokinin 6-benzylaminopurine (BAP) individually and in combination. Supplementation with BAP alone resulted in the highest values for root length, root dry weight, shoot length, and numbers of nodes, leaves, and ramifications. Treatment with IBA alone or with IBA + BAP resulted in a ~50% increase in shoot fresh weight. The production of secondary metabolites was affected only by the addition of cytokinin, which resulted in a ~40% increase in the total yield of essential oils (EOs). Similar trends were observed for yields of the major EO components (menthone, menthol, pulegone, and menthofuran). Our findings demonstrate that the application of growth regulators increases EO production and biomass concomitantly in an herbaceous species rich in commercially valuable terpenes.
文摘AIM:To examine the antiulcerogenic effects of various extracts of Mentha arvensis Linn on acid,ethanol and pylorus ligated ulcer models in rats and mice.METHODS:Various crude extracts of petroleum ether,chloroform,or aqueous at a dose of 2 g/kg po did not produce any signs or symptoms of toxicity in treated animals.In the pyloric ligation model oral administration of different extracts such as petroleum ether,chloroform and aqueous at 375 mg/kg po,standard drug ranitidine 60 mg/kg po and control group 1 Tween 80,5 mL/kg po to separate groups of Wister rats of either sex(n = 6) was performed.Total acidity,ulcer number,scoring,incidence,area,and ulcer index were assessed.RESULTS:There was a decrease in gastric secretion and ulcer index among the treated groups i.e.petroleum ether(53.4),chloroform(59.2),aqueous(67.0) and in standard drug(68.7) when compared to the negative control.In the 0.6 mol/L HCl induced ulcer model in rats(n = 6) there was a reduction in ulcerative score in animals receiving petroleum ether(50.5),chloroform(57.4),aqueous(67.5) and standard.drug(71.2) when compared to the negative control.In the case of the 90 ethanol-induced ulceration model(n = 6) in mice,there was a decrease in ulcer score in test groups of petroleum ether(53.11),chloroform(62.9),aqueous(65.4) and standard drug ranitidine(69.7) when compared to the negative control.It was found that pre-treatment with various extracts of Mentha arvensis Linn in three rat/mice ulcer models ie ibuprofen plus pyloric ligation,0.6 mol/L HCl and 90 ethanol produced significant action against acid secretion(49.3 ± 0.49 vs 12.0 ± 0.57,P < 0.001).Pre-treatment with various extracts of Mentha arvensis Linn showed highly-significant activity against gastric ulcers(37.1 ± 0.87 vs 12.0 ± 0.57,P < 0.001).CONCLUSION:Various extracts of Mentha arvensis Linn.375 mg/kg body weight clearly shows a protective effect against acid secretion and gastric ulcers in ibuprofen plus pyloric ligation,0.6 mol/L HCl induced and 90 ethanol-induced ulcer models.
