Objective: To investigate the chemical composition, in vitro antibacterial and antifungal activity of Mentha longifolia L. essential oils using aqueous extract. Methods: Mentha longifolia L. essential oils were extrac...Objective: To investigate the chemical composition, in vitro antibacterial and antifungal activity of Mentha longifolia L. essential oils using aqueous extract. Methods: Mentha longifolia L. essential oils were extracted using hydrodistillation with Clevenger apparatus for 3 h and the yield of the essential oil was calculated. Essential oils were qualitatively and quantitatively analyzed with gas chromatography and gas chromatography-mass spectrometer. For these essential oils, antibacterial and antifungal activity against human pathogens were evaluated. Results: Nineteen chemical constituents representing 99.72% of the essential oil were found, comprising menthone (39.55%), isopulegone (30.49%), eucalyptol (10.38%), and α-terpineol (3.15%);these were major components, and others were minor components. The essential oil showed strong antibacterial activity against Staphylococcus aureus [(35.24 ± 0.13) mm], Enterococcus faecalis [(32.12 ± 0.12) mm] and Bacillus cereus [(30.06 ± 0.04) mm], as well as antifungal activity against Aspergillus flavus [(38.02 ± 0.06) mm], Alternaria alternaria [(35.26 ± 0.12) mm], and Penicillum spp ((34.14 ± 0.02) mm)Conclusions: It seems that the essential oils derived from the Mentha longifolia L. species could be used as a natural source of antimicrobial agents.展开更多
文摘Objective: To investigate the chemical composition, in vitro antibacterial and antifungal activity of Mentha longifolia L. essential oils using aqueous extract. Methods: Mentha longifolia L. essential oils were extracted using hydrodistillation with Clevenger apparatus for 3 h and the yield of the essential oil was calculated. Essential oils were qualitatively and quantitatively analyzed with gas chromatography and gas chromatography-mass spectrometer. For these essential oils, antibacterial and antifungal activity against human pathogens were evaluated. Results: Nineteen chemical constituents representing 99.72% of the essential oil were found, comprising menthone (39.55%), isopulegone (30.49%), eucalyptol (10.38%), and α-terpineol (3.15%);these were major components, and others were minor components. The essential oil showed strong antibacterial activity against Staphylococcus aureus [(35.24 ± 0.13) mm], Enterococcus faecalis [(32.12 ± 0.12) mm] and Bacillus cereus [(30.06 ± 0.04) mm], as well as antifungal activity against Aspergillus flavus [(38.02 ± 0.06) mm], Alternaria alternaria [(35.26 ± 0.12) mm], and Penicillum spp ((34.14 ± 0.02) mm)Conclusions: It seems that the essential oils derived from the Mentha longifolia L. species could be used as a natural source of antimicrobial agents.