The generation of induced pluripotent stem cells through somatic cell reprogramming requires a global reorganization of cellular functions.This reorganization occurs in a multi-phased manner and involves a gradual rev...The generation of induced pluripotent stem cells through somatic cell reprogramming requires a global reorganization of cellular functions.This reorganization occurs in a multi-phased manner and involves a gradual revision of both the epigenome and transcriptome.Recent studies have shown that the large-scale transcriptional changes observed during reprogramming also apply to long noncoding RNAs(lncR NAs),a type of traditionally neglected RNA species that are increasingly viewed as critical regulators of cellular function.Deeper understanding of lncR NAs in reprogramming may not only help to improve this process but also have implications for studying cell plasticity in other contexts,such as development,aging,and cancer.In this review,we summarize the current progress made in profiling and analyzing the role of lncR NAs in various phases of somatic cell reprogramming,with emphasis on the re-establishment of the pluripotency gene network and X chromosome reactivation.展开更多
Phosphatase of regenerating liver 3(PRL3)promotes colorectal cancer(CRC)metastasis by inducing epithelial-to-mesenchymal transition.Mesenchymal-to-epithelial transition(MET),the opposite of epithelial-to-mesenchymal t...Phosphatase of regenerating liver 3(PRL3)promotes colorectal cancer(CRC)metastasis by inducing epithelial-to-mesenchymal transition.Mesenchymal-to-epithelial transition(MET),the opposite of epithelial-to-mesenchymal transition,has been proposed as a mechanism for the establishment of metastatic neoplasms.However,the molecular mechanism of MET remains unclear.Here,we show that miR-203a-3p derived from hepatocyte exosomes inhibits Src expression,reduces epidermal growth factor receptor activity and downstream signaling pathways,and increases E-cadherin expression,which is a typical mesenchymal cell marker of MET,in CRC cells.These results show the important role of epidermal growth factor receptor in CRC cell MET.展开更多
基金supported by the National Key R&D Program of China(Grant Nos.2016YFA0100701,2016YFA0100102,2018YFA0106903,and 2016YFA0100300)the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDA16030502)+8 种基金the Youth Innovation Promotion Association of the Chinese Academy of Sciences(Grant No.2015294)the National Natural Science Foundation of China(Grant Nos.31671537,31571524,and 31501192)the Natural Science Foundation of Guangdong Province(Grant No.2018B030306042)the Guangdong Province Science and Technology Program(Grant Nos.2014A030312001,2016A050503037,2016B030229007,and 2017B050506007)the Science and Technology Planning Project of Guangdong Province(Grant No.2017B030314056)the Pearl River Science and Technology Nova Program of Guangzhou(Grant No.201610010107)the Guangzhou Science and Technology Program(Grant No.201807010066),Chinasupported by a President’s International Fellowship Initiative program from the Chinese Academy of Sciencessupported by a Pearl River Overseas Young Talents Postdoctoral Fellowship
文摘The generation of induced pluripotent stem cells through somatic cell reprogramming requires a global reorganization of cellular functions.This reorganization occurs in a multi-phased manner and involves a gradual revision of both the epigenome and transcriptome.Recent studies have shown that the large-scale transcriptional changes observed during reprogramming also apply to long noncoding RNAs(lncR NAs),a type of traditionally neglected RNA species that are increasingly viewed as critical regulators of cellular function.Deeper understanding of lncR NAs in reprogramming may not only help to improve this process but also have implications for studying cell plasticity in other contexts,such as development,aging,and cancer.In this review,we summarize the current progress made in profiling and analyzing the role of lncR NAs in various phases of somatic cell reprogramming,with emphasis on the re-establishment of the pluripotency gene network and X chromosome reactivation.
基金This work was supported by Grant from Key Laboratory of Malignant Tumor Molecular Mechanism and Translational Medicine of Guangzhou Bureau of Science and Information TechnologyGrant from the Key Laboratory of Malignant Tumor Gene Regulation and Target Therapy of Guangdong Higher Education Institutes+3 种基金and financially supported by the grants from National Natural Science Foundation of China(nos.81602539,81702902,81602125)Natural Science Foundation of Guangdong Province(no.2016A030310183)International Science and Technology Cooperation Program of Guangdong Province(no.2015A050502021)Science and Technology of Guangdong Province(no.201400000004-5).
文摘Phosphatase of regenerating liver 3(PRL3)promotes colorectal cancer(CRC)metastasis by inducing epithelial-to-mesenchymal transition.Mesenchymal-to-epithelial transition(MET),the opposite of epithelial-to-mesenchymal transition,has been proposed as a mechanism for the establishment of metastatic neoplasms.However,the molecular mechanism of MET remains unclear.Here,we show that miR-203a-3p derived from hepatocyte exosomes inhibits Src expression,reduces epidermal growth factor receptor activity and downstream signaling pathways,and increases E-cadherin expression,which is a typical mesenchymal cell marker of MET,in CRC cells.These results show the important role of epidermal growth factor receptor in CRC cell MET.
