The freshwater fish Carassius auratus was chosen as an experimental subject, and their hepatic biochemical responses to the medium-term exposure of Benzo（k）fluoranthene （BkF） alone and in combination with PCB 118 ...The freshwater fish Carassius auratus was chosen as an experimental subject, and their hepatic biochemical responses to the medium-term exposure of Benzo（k）fluoranthene （BkF） alone and in combination with PCB 118 and dichlorodiphenyltrichloroethane （DDT） were investigated by measuring the reduced glutathione （GSH）, glutathione S-transferase （GST）, and thiobarbituric acid reactive substances （TBARS）, to assess sublethal effects. The hepatic GSH content was significantly inhibited by organic pollutants, alone and in mixtures, while the TBARS content was significantly induced after three days of exposure. Bell-shaped concentration-response charts of GST activities were obtained. Significant dose-response relationships were found for hepatic GSH and TBARS contents of all concentrations and for the GST activity, except at the highest concentration. The GSH content, GST activity, and TBARS content in Carassius auratus were confirmed as useful biomarkers of exposure to organic pollutions.展开更多
目的纯化黑线毛足鼠和金仓鼠血清IgG,制备兔抗金仓鼠和黑线毛足鼠血清IgG的抗血清。方法用Hitrap Protein G亲和层析纯化黑线毛足鼠和金仓鼠血清IgG,经SDS-PAGE电泳鉴定纯度,标准免疫方法免疫兔子制备抗血清。结果黑线毛足鼠和金仓鼠血...目的纯化黑线毛足鼠和金仓鼠血清IgG,制备兔抗金仓鼠和黑线毛足鼠血清IgG的抗血清。方法用Hitrap Protein G亲和层析纯化黑线毛足鼠和金仓鼠血清IgG,经SDS-PAGE电泳鉴定纯度,标准免疫方法免疫兔子制备抗血清。结果黑线毛足鼠和金仓鼠血清IgG对protein G有很高的亲合性,用Hitrap Protein G亲和层析纯化,得到高纯度黑线毛足鼠和金仓鼠IgG,利用纯化的IgG作抗原制备了高效价的抗血清,免疫双扩散测定效价达1∶32和1∶16。结论证实黑线毛足鼠和金仓鼠IgG和Protein G具有很高的亲和性,Protein G亲和层析是纯化黑线毛足鼠和金仓鼠IgG有效的方法之一,制备了黑线毛足鼠和金仓鼠IgG的抗血清。展开更多
人类活动产生的噪声污染对动物和人类的影响正受到日益增多的关注。本文以雄性成年金色中仓鼠为实验动物模型,探讨了北京市主干道交通噪声对其焦虑行为及血象、应激生理的影响。分别以北京主干道噪声(80±10)d B SPL暴露为实验组,...人类活动产生的噪声污染对动物和人类的影响正受到日益增多的关注。本文以雄性成年金色中仓鼠为实验动物模型,探讨了北京市主干道交通噪声对其焦虑行为及血象、应激生理的影响。分别以北京主干道噪声(80±10)d B SPL暴露为实验组,实验室环境噪声(50±4)d B SPL暴露为对照组,噪声处理动物1 h后进行旷场行为学测试,然后取血对比观测两组鼠血液学指标和应激激素、抗氧化酶活性等生理指标的变化。结果显示道路交通噪声没有导致仓鼠出现明显的焦虑行为;实验组血小板数显著低于对照组(P=0.044),其他血象指标两组间差异不显著;噪声对血清皮质醇、谷丙转氨酶、谷草转氨酶影响不显著;实验组的血清谷胱甘肽过氧化物酶(GSH-Px)活性极显著低于对照组(P<0.001),但超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、总抗氧化能力(TAOC)和丙二醛(MDA)水平两组间差异不显著;血清溶菌酶活性实验组降低较明显,接近显著水平(P=0.0507)。我们的结果显示道路交通噪声胁迫导致了金色中仓鼠血象指标发生了变化,这提示北京市主干道交通噪声刺激对金色中仓鼠生理功能产生了一定的副作用。展开更多
基金supported by the Special Fund for Public Welfare Industry of Ministry of Water Resources of China(Grant No201001056)the Project of Jiangxi Provincial Department of Science and Technology (Grant No2010BSA20300)the Project of Jiangxi Provincial Department of Education(Grants No GJJ11246 and GJJ11636)
文摘The freshwater fish Carassius auratus was chosen as an experimental subject, and their hepatic biochemical responses to the medium-term exposure of Benzo（k）fluoranthene （BkF） alone and in combination with PCB 118 and dichlorodiphenyltrichloroethane （DDT） were investigated by measuring the reduced glutathione （GSH）, glutathione S-transferase （GST）, and thiobarbituric acid reactive substances （TBARS）, to assess sublethal effects. The hepatic GSH content was significantly inhibited by organic pollutants, alone and in mixtures, while the TBARS content was significantly induced after three days of exposure. Bell-shaped concentration-response charts of GST activities were obtained. Significant dose-response relationships were found for hepatic GSH and TBARS contents of all concentrations and for the GST activity, except at the highest concentration. The GSH content, GST activity, and TBARS content in Carassius auratus were confirmed as useful biomarkers of exposure to organic pollutions.
文摘目的纯化黑线毛足鼠和金仓鼠血清IgG,制备兔抗金仓鼠和黑线毛足鼠血清IgG的抗血清。方法用Hitrap Protein G亲和层析纯化黑线毛足鼠和金仓鼠血清IgG,经SDS-PAGE电泳鉴定纯度,标准免疫方法免疫兔子制备抗血清。结果黑线毛足鼠和金仓鼠血清IgG对protein G有很高的亲合性,用Hitrap Protein G亲和层析纯化,得到高纯度黑线毛足鼠和金仓鼠IgG,利用纯化的IgG作抗原制备了高效价的抗血清,免疫双扩散测定效价达1∶32和1∶16。结论证实黑线毛足鼠和金仓鼠IgG和Protein G具有很高的亲和性,Protein G亲和层析是纯化黑线毛足鼠和金仓鼠IgG有效的方法之一,制备了黑线毛足鼠和金仓鼠IgG的抗血清。
文摘人类活动产生的噪声污染对动物和人类的影响正受到日益增多的关注。本文以雄性成年金色中仓鼠为实验动物模型,探讨了北京市主干道交通噪声对其焦虑行为及血象、应激生理的影响。分别以北京主干道噪声(80±10)d B SPL暴露为实验组,实验室环境噪声(50±4)d B SPL暴露为对照组,噪声处理动物1 h后进行旷场行为学测试,然后取血对比观测两组鼠血液学指标和应激激素、抗氧化酶活性等生理指标的变化。结果显示道路交通噪声没有导致仓鼠出现明显的焦虑行为;实验组血小板数显著低于对照组(P=0.044),其他血象指标两组间差异不显著;噪声对血清皮质醇、谷丙转氨酶、谷草转氨酶影响不显著;实验组的血清谷胱甘肽过氧化物酶(GSH-Px)活性极显著低于对照组(P<0.001),但超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、总抗氧化能力(TAOC)和丙二醛(MDA)水平两组间差异不显著;血清溶菌酶活性实验组降低较明显,接近显著水平(P=0.0507)。我们的结果显示道路交通噪声胁迫导致了金色中仓鼠血象指标发生了变化,这提示北京市主干道交通噪声刺激对金色中仓鼠生理功能产生了一定的副作用。
