Objective： To investigate the relationship between matrix metalloproteinases-9 （MMP-9） and sialyl Lewis X （CD15s） antigen in invasion and metastasis of gastric carcinoma. Methods： Expression of CD105, MMP-9 and ...Objective： To investigate the relationship between matrix metalloproteinases-9 （MMP-9） and sialyl Lewis X （CD15s） antigen in invasion and metastasis of gastric carcinoma. Methods： Expression of CD105, MMP-9 and CD15s in 47 cases of gastric carcinoma undergone radical surgery were evaluated by SP immunohistochemical staining using the respective monoclonal antibody. The microvessel density （MVD） marked with CD105 was detected. Correlation between MVD and MMP-9, CD15s was also statistically analyzed. Results： The MVD in both MMP-9 and CD15s positive expression group was higher significantly than that in MMP-9 or CD15s positive expression alone group, and it was also significantly higher than that in both MMP-9 and CD15s negative expression group. Conclusion： MMP-9 is a marker of invasion and CD15s is a marker of metastasis in gastric carcinoma. Combining detection of MMP-9 and CD15s has certain clinical significance for diagnosis, treatment and assessing the prognosis for gastric caner.展开更多
To investigate the effects of mechanical factors on matrix metalloproteinase9(MMP-9) expressions in rat bone marrow-derived mesenchymal stem cells(MSCs) and possible mechanism signal.Rat bone marrow MSCs were isolated...To investigate the effects of mechanical factors on matrix metalloproteinase9(MMP-9) expressions in rat bone marrow-derived mesenchymal stem cells(MSCs) and possible mechanism signal.Rat bone marrow MSCs were isolated and cultured,then,exposed to laminar shear stress展开更多
Objective: To investigate the changes of the plasma level of MMP-9 (Matrix Metalloproteinase-9, MMP-9) in the patients with abdominal aortic aneurysms (AAAs) before and after the treatment, and evaluate the significan...Objective: To investigate the changes of the plasma level of MMP-9 (Matrix Metalloproteinase-9, MMP-9) in the patients with abdominal aortic aneurysms (AAAs) before and after the treatment, and evaluate the significance of MMP-9 in the pathogenesis of AAAs. Methods: Blood samples of 35 patients with AAAs and 10 patients with the arterial occlusive diseases (AODs) , which enrolled into the Vascular Surgery Center of Colonge University Hospital from February to August of 2002, were collected before and one month after surgical repair or less-invasive endovascular exclusion. The plasma concentrations of MMP-9 of all the collected samples were measured by means of enzyme-linked immunosorbent assay(ELISA), and compared between the two groups patients at different time point. Results: The mean plasma concentration of MMP-9 of AAAs was significantly higher than that of AODs prior to treatment [(90.3±9.1) ng/ml vs (23.6±7.3) ng/ml, P<0.05], and no apparent difference was showed in the patients with AODs [(23.6±7.3) ng/ml vs (25.3±5.8) ng/ml, P>0.05)] before and after the surgical bypass operation. However, in the patients with AAAs the plasma concentration of MMP-9 was apparently decreased one month after the surgical repair or endovascular exclusion compared with before [(28.6±8.4) ng/ml vs (90.3±9.1) ng/ml, P<0.05)]. No meaningful difference of the mean plasma MMP-9 concentration was seen between two groups after the both being successfully treated [(28.6±8.4) ng/ml vs (25.3±5.8) ng/ml, P>0.05]. Conclusion: Apparent elevation of plasma concentration of MMP-9 in the AAAs and its dramatic decrease after being treated implicated that MMP-9 might play an important role in the formation and development of AAAs. Meanwhile, to investigate the changes of MMP-9 level of AAAs could provide an practical way to facilitate the earlier diagnosis and long term surveillance for AAAs. More importantly, pharmacologic prevention and treatment of AAAs, in which the MMP-9 serves as effective target, might be possible in the future.展开更多
支持等式测试的标识加密(identity-based encryption with equality test, IBEET)体制解决了传统等式测试方案中证书管理的问题,得到了广泛的关注.但现有的IBEET体制难以抵抗渗透攻击,且都是基于国外密码算法设计,不具有自主知识产权....支持等式测试的标识加密(identity-based encryption with equality test, IBEET)体制解决了传统等式测试方案中证书管理的问题,得到了广泛的关注.但现有的IBEET体制难以抵抗渗透攻击,且都是基于国外密码算法设计,不具有自主知识产权.基于此,提出一种支持等式测试并具有密码逆向防火墙的SM9标识加密方案(SM9 identity-based encryption scheme with equality test and cryptographic reverse firewalls, SM9-IBEET-CRF).该方案在用户与云服务器的上行信道间部署密码逆向防火墙(cryptographic reverse firewalls,CRF),对用户发出的信息执行重随机化以达到抵抗渗透攻击的作用.该方案拓展国密算法SM9至IBEET领域中,提升其运行效率并丰富国密算法在云计算领域的研究.给出了SM9-IBEET-CRF的形式化定义和安全模型,并在随机预言机模型中考虑2种不同的敌手将此方案在选择密文攻击下的不可区分性与单向性分别形式化地规约到BDH困难假设上.同时,该方案通过考虑第3种敌手证明CRF的部署为其带来维持功能性、保留安全性以及抵抗渗透性.实验仿真和分析结果展示了该方案的有效性.展开更多
文摘Objective： To investigate the relationship between matrix metalloproteinases-9 （MMP-9） and sialyl Lewis X （CD15s） antigen in invasion and metastasis of gastric carcinoma. Methods： Expression of CD105, MMP-9 and CD15s in 47 cases of gastric carcinoma undergone radical surgery were evaluated by SP immunohistochemical staining using the respective monoclonal antibody. The microvessel density （MVD） marked with CD105 was detected. Correlation between MVD and MMP-9, CD15s was also statistically analyzed. Results： The MVD in both MMP-9 and CD15s positive expression group was higher significantly than that in MMP-9 or CD15s positive expression alone group, and it was also significantly higher than that in both MMP-9 and CD15s negative expression group. Conclusion： MMP-9 is a marker of invasion and CD15s is a marker of metastasis in gastric carcinoma. Combining detection of MMP-9 and CD15s has certain clinical significance for diagnosis, treatment and assessing the prognosis for gastric caner.
文摘To investigate the effects of mechanical factors on matrix metalloproteinase9(MMP-9) expressions in rat bone marrow-derived mesenchymal stem cells(MSCs) and possible mechanism signal.Rat bone marrow MSCs were isolated and cultured,then,exposed to laminar shear stress
文摘Objective: To investigate the changes of the plasma level of MMP-9 (Matrix Metalloproteinase-9, MMP-9) in the patients with abdominal aortic aneurysms (AAAs) before and after the treatment, and evaluate the significance of MMP-9 in the pathogenesis of AAAs. Methods: Blood samples of 35 patients with AAAs and 10 patients with the arterial occlusive diseases (AODs) , which enrolled into the Vascular Surgery Center of Colonge University Hospital from February to August of 2002, were collected before and one month after surgical repair or less-invasive endovascular exclusion. The plasma concentrations of MMP-9 of all the collected samples were measured by means of enzyme-linked immunosorbent assay(ELISA), and compared between the two groups patients at different time point. Results: The mean plasma concentration of MMP-9 of AAAs was significantly higher than that of AODs prior to treatment [(90.3±9.1) ng/ml vs (23.6±7.3) ng/ml, P<0.05], and no apparent difference was showed in the patients with AODs [(23.6±7.3) ng/ml vs (25.3±5.8) ng/ml, P>0.05)] before and after the surgical bypass operation. However, in the patients with AAAs the plasma concentration of MMP-9 was apparently decreased one month after the surgical repair or endovascular exclusion compared with before [(28.6±8.4) ng/ml vs (90.3±9.1) ng/ml, P<0.05)]. No meaningful difference of the mean plasma MMP-9 concentration was seen between two groups after the both being successfully treated [(28.6±8.4) ng/ml vs (25.3±5.8) ng/ml, P>0.05]. Conclusion: Apparent elevation of plasma concentration of MMP-9 in the AAAs and its dramatic decrease after being treated implicated that MMP-9 might play an important role in the formation and development of AAAs. Meanwhile, to investigate the changes of MMP-9 level of AAAs could provide an practical way to facilitate the earlier diagnosis and long term surveillance for AAAs. More importantly, pharmacologic prevention and treatment of AAAs, in which the MMP-9 serves as effective target, might be possible in the future.
文摘支持等式测试的标识加密(identity-based encryption with equality test, IBEET)体制解决了传统等式测试方案中证书管理的问题,得到了广泛的关注.但现有的IBEET体制难以抵抗渗透攻击,且都是基于国外密码算法设计,不具有自主知识产权.基于此,提出一种支持等式测试并具有密码逆向防火墙的SM9标识加密方案(SM9 identity-based encryption scheme with equality test and cryptographic reverse firewalls, SM9-IBEET-CRF).该方案在用户与云服务器的上行信道间部署密码逆向防火墙(cryptographic reverse firewalls,CRF),对用户发出的信息执行重随机化以达到抵抗渗透攻击的作用.该方案拓展国密算法SM9至IBEET领域中,提升其运行效率并丰富国密算法在云计算领域的研究.给出了SM9-IBEET-CRF的形式化定义和安全模型,并在随机预言机模型中考虑2种不同的敌手将此方案在选择密文攻击下的不可区分性与单向性分别形式化地规约到BDH困难假设上.同时,该方案通过考虑第3种敌手证明CRF的部署为其带来维持功能性、保留安全性以及抵抗渗透性.实验仿真和分析结果展示了该方案的有效性.
