The fermentation with mold metarrhizium anisopliae has been carried out to determine the relationship between mycelial morphology and growth.Like the biomass concentration,the mycelial morphology seems to be coupled t...The fermentation with mold metarrhizium anisopliae has been carried out to determine the relationship between mycelial morphology and growth.Like the biomass concentration,the mycelial morphology seems to be coupled to growth phases:the fractal dimension,a feature calculated from the morphological images,increases during the late lag phase and the early exponential phase,and culminates at a value near 2.4 at half of the exponential phase.This can be attributed to the marked change of rough and smooth pellet proportions during growth and the change can be well expressed by the fractal analysis of mycelial morphology.The mycelial morphology is also strongly related to the biotransformation activity:a mycelial sample was withdrawn from the culture to use for fractal analysis before the steroid substrate (16α,17α epoxy 4 pregnene 3,20 dione) was added;the higher fractal dimension corresponds to the higher conversion rate of steroid biotransformation.And the maximum of fractal dimension appeared prior to the maximum of biomass concentration.It is therefore possible to utilize the fractal dimension curve to supervise the fermentation process more timely and availably than to use the conventional biomass curve to do.展开更多
MetarhizosidesA-G(1-7),seven new polysubstituted phenyl glucosides,were isolated from the extracts of solid rice medium of a marine-derived fungus Metarrhizium anisopliae.Compounds 1-7 all contain a polysubstituted ph...MetarhizosidesA-G(1-7),seven new polysubstituted phenyl glucosides,were isolated from the extracts of solid rice medium of a marine-derived fungus Metarrhizium anisopliae.Compounds 1-7 all contain a polysubstituted phenyl group and the sugar unit is identified as 4'-O-methyl-β-D-glucopyranose.Their structures were elucidated by NMR spectroscopy and chemical method.These compounds were evaluated for anti-inflammatory activity by using LPS-stimulated murine macrophage RAW 264.7 cells and the cytotoxicities against four human cancer cell lines.展开更多
基金National Natural Science Foundation of China forYoung Researcher !(No.2 960 60 0 8) Tianjin Natural ScienceFoundation!
文摘The fermentation with mold metarrhizium anisopliae has been carried out to determine the relationship between mycelial morphology and growth.Like the biomass concentration,the mycelial morphology seems to be coupled to growth phases:the fractal dimension,a feature calculated from the morphological images,increases during the late lag phase and the early exponential phase,and culminates at a value near 2.4 at half of the exponential phase.This can be attributed to the marked change of rough and smooth pellet proportions during growth and the change can be well expressed by the fractal analysis of mycelial morphology.The mycelial morphology is also strongly related to the biotransformation activity:a mycelial sample was withdrawn from the culture to use for fractal analysis before the steroid substrate (16α,17α epoxy 4 pregnene 3,20 dione) was added;the higher fractal dimension corresponds to the higher conversion rate of steroid biotransformation.And the maximum of fractal dimension appeared prior to the maximum of biomass concentration.It is therefore possible to utilize the fractal dimension curve to supervise the fermentation process more timely and availably than to use the conventional biomass curve to do.
基金This work was financially supported by the Program for Changjiang Scholars of Ministry of Education of the People's Republic of China(No.T2016088)the National Natural Science Foundation for Distinguished Young Scholars(No.81725021)+2 种基金the National Natural Science Foundation of China(No.31900288)the Program from the(hina Postdoctoral Science Foundation(No.2019M652660)the Integrated Innovative Team for Major Human Diseases Program of Tongji Medical College(HUST,China).
文摘MetarhizosidesA-G(1-7),seven new polysubstituted phenyl glucosides,were isolated from the extracts of solid rice medium of a marine-derived fungus Metarrhizium anisopliae.Compounds 1-7 all contain a polysubstituted phenyl group and the sugar unit is identified as 4'-O-methyl-β-D-glucopyranose.Their structures were elucidated by NMR spectroscopy and chemical method.These compounds were evaluated for anti-inflammatory activity by using LPS-stimulated murine macrophage RAW 264.7 cells and the cytotoxicities against four human cancer cell lines.
