A change of phenolic acids content in poplar leaves induced by methyl sali-cylate and methyl jasmonate

The contents of seven different phenolic acids such as gallic acid, catechinic acid, pyrocatechol, caffeic acid, coumaric acid, ferulic acid and benzoic acid in the poplar leaves （Populus Simonii×Populus Pyramibalis c.v and Populus deltoids） suffocated by Methyl jasmonate （MeJA） and Methyl salicylate （MeSA） were monitored for analyzing their functions in interplant communications by using high-pressure liquid chromatography （HPLC）.The results showed that the contents of phenolic acids had obviously difference in leaves exposed to either MeSA or MeJA.When P.deltoides leaves exposed to MeJA or MeSA, the level of gallic acid, coumaric acid, caffeic acid, ferulic acid and benzoic acid was increased, gallic acid in leaves treated with MeJA comes to a peak at 24 h while to a peak at 12-d having leaves treated with MeSA.When P.Simonii ×P.Pyramibalis c.v leaves were exposed to MeJA or MeSA, the level of gallic acid, pyrocatechol and ferulic acid was increased; The catechinic acid and benzoic acid had a little drop; The caffeic acid and coumaric acid were undetected in both suffocated and control leaves.This changed pattern indicated that MeJA and MeSA can act as airborne signals to induce defense response of plants.

Increased Endogenous Methyl Jasmonate Altered Leaf and Root Development in Transgenic Soybean Plants

Methyl jasmonate （MeJA） is a plant-signaling molecule that regulates plant morphogenesis and expression of plant defense genes. To determine the role of the endogenous MeJA levels in the development of plants, transgenic soybean [Glycine max （L.） Merrill] plants harboring NTR1 gene encoding for jasmonic acid carboxyl methyltransferase （JMT） were produced. The activation of NTR1 gene expression resulted in the production of MeJA. Overexpression of the NTR1 cDNA under the regulation of cauliflower mosaic virus （CaMV） 35S promoter in the transgenic soybean plants was confirmed using Northern blot analysis. The significant differences in leaf and root growth patterns were observed between the transgenic plants and the wild-type plants. The leaves of the transgenic plants were slightly elongated in length but dramatically narrowed in width compared with the nontrans-formed wild-type plants. In addition, elongation of primary root was inhibited in the overexpressed transgenic soybean plantlets, whereas the development of lateral root was stimulated relative to the nontransformed plants. The leaves of the transgenic plants showed 2-2.5-fold higher levels of MeJA than the control plants. These results indicated that the increased endogenous levels of MeJA is involved in regulation of morphogenesis in soybean plants.

Methyl Jasmonate Reduces Chilling Injury and Maintains Postharvest Quality in Peaches

Zhaohui peaches(Prunus persica Batsch)were treated with 0(CK), 1, 10 or 100 μmol L-1 methyl jasmonate(MeJA)vapor at 20℃ for 24 h before stored at 0℃ for 35 d. The untreated fruits showed chilling injury(CD symptoms after 4 wk of storage, as indicated by increased fruit firmness and reduced ex-tractable juice, which is referred as leatheriness. Treatment with 1 and 10 μmol L-1 MeJA promoted normal ripening and softening, maintained higher levels of extractable juice, titratable acidity, pectinesterase(PE)and polygalacturonase(PC)activities, inhibited increases in fruit fresh weight loss, decay incidence, electrolyte leakage and MDA content, and improved color development, thereby preventing chilling injury symptoms development and maintaining edible quality. MeJA treatment also delayed the climacteric rise in respiratory rate, but promoted ethylene production during the later period of cold storage, suggesting that ethylene may involve in CI development of peaches. These results indicate that 1 and 10 μmol L-1 MeJA treatments could be used to reduce CI development and decay incidence in peaches.

Application of methyl jasmonate postharvest maintains the quality of Nanguo pears by regulating mitochondrial energy metabolism

The present study was conducted to investigate the effects of methyl jasmonate(Me JA)dipping treatment on mitochondrial energy metabolism and quality parameters of Nanguo pears during room temperature storage.The results showed that Me JA treatment suppressed the respiration rate and weight loss,and maintained the flesh firmness of Nanguo pears.Me JA also effectively maintained the content of ascorbic acid and titratable acidity in the fruit.Furthermore,the activities of H^(+)-ATPase,Ca^(2+)-ATPase,succinate dehydrogenase(SDH)and cytochrome C oxidase(CCO)of the Me JA-treated fruit were significantly higher than those of the untreated fruit.The contents of adenosine triphosphate(ATP)and adenosine diphosphate(ADP)and the energy charge were also enhanced by Me JA treatment.These results suggest that postharvest Me JA treatment could maintain the quality of Nanguo pears,in part by modulating mitochondrial energy metabolism during room temperature storage.

Impact of Methyl Jasmonate on Antioxidant Activity and Some Biochemical Aspects of Maize Plant Grown under Water Stress Condition

A pot-culture study was undertaken to investigate the possible role of Methyl jasmonate (MeJA)-treatment on the earlier vegetative growth stage and different chemical constituents of maize cultivar (Giza-2) plants subjected to water stress. The grains were divided into two groups: first group was pre-soaked in water, and the second one was pre-soaked in 50 μM MeJA for 8 h. The plants were subjected to different levels of water field capacity (WFC) 65%, 55% and 45%. The results showed that pre-soaking maize grains with MeJA led to increases in plant growth criteria evident in terms of plant height, fresh and dry weight of plant. The pigment levels concomitantly with total carbohydrates, total soluble sugar, polysaccharides, as well as free amino acids, proline and total protein content were markedly increased. Moreover, the application of the investigated MeJA significantly improved growth hormone in terms of IAA. In contrast ABA level was markedly declined in maize plant. The activities of oxidative CAT, POX and SOD were also increased with MeJA. In addition, the N, P and K content was increased significantly in shoot. As a conclusion, soaking maize grains with MeJA could alleviate the harmful effects of water stress.

Regulatory effect of salicylic acid and methyl jasmonate supplementation on ergosterol production in Hericium erinaceus mycelia

We evaluated the biomass and ergosterol content of Hericium erinaceus mycelium, and extracellular enzyme activities in H. erinaceus liquid culture following salicylic acid（SA） and methyl jasmonic acid（Me JA）supplementation. The optimal SA concentration was100 lmoláL-1, where the highest ergosterol content of 2.33 mgág-1was obtained following 6-day cultivation with100 lmoláL-1SA supplementation, and which was significantly higher than the unsupplemented control（p ／ 0.01）. Following 4-day supplementation with50 lmoláL-1Me JA, the highest ergosterol content obtained was 1.988 mgág-1, which was 25.8 % higher than the unsupplemented control. Our data indicate that SA and Me JA supplementation improves ergosterol content in H.erinaceus mycelium.

Effects of Methyl Jasmonate on the Composition of Volatile Compounds in Pyropia yezoensis

Volatile organic compounds in marine algae have been reported to comprise characteristic flavor of algae and play an important role in their growth, development and defensive response. Yet their biogeneration remain largely unknown. Here we studied the composition of volatile compouds in Pyropia yezoensis and their variations in response to methyl jasmonate(MeJA) and diethyldithiocarbamic acid(DIECA) treatment using gas chromatography-mass spectrometry(GC-MS). A total of 44 compounds belonging to the following chemical classes(n) were identified, including aldehydes(11), alcohols(8), acids and esters(6), alkanes(5), ketones(5), alkenes(3), and S-or N-containing miscellaneous compounds(6). External treatment with plant hormone MeJA increased the content of 1-dodecanol, 4-heptenal, and 2-propenoic acid-2-methyl dodecylester, but decreased the content of phytol, 3-heptadecene, 2-pentadecanone, and isophytol. When pretreated with DIECA, an inhibitor of the octadecanoid pathway leading to the biosynthesis of endogeneous jasmonates and some secondary metabolites, phytol and isophytol were increased, while 4-heptenal, 1-dodecanol, and 2-propenoic acid-2-methyl dodecylester were decreased, both of which were negatively correlated with their variations under MeJA treatment. Collectively, these results suggest that MeJA does affect the volatile composition of P. yezoensis, and the octadecanoid pathway together with endogenous jasmonate pathway may be involved in the biosynthesis of volatile compounds, thereby providing some preliminary envision on the composition and biogeneration of volatile compounds in P. yezoensis.

Synergistic Effects of a Night Temperature Shift and Methyl Jasmonate on the Production of Anthocyanin in Red Leaf Lettuce

The production of a secondary metabolite such as anthocyanin is coordinately regulated by plant intrinsic factors and influenced by multiple environmental factors. In red leaf lettuce, the red pigment component anthocyanin is important for the commercial value of the crop, but its synchronous regulation by multiple factors is not well understood. Here, we examined the synergistic effects of a night temperature shift and methyl jasmonate (MJ) on the production of anthocyanin in red leaf lettuce. Low or high night temperature treatment for 3 days just before harvesting induced the production of anthocyanin without affecting plant biomass. Temperature-dependent activation of anthocyanin accumulation was accelerated by treating with MJ. Night temperature shifts and MJ triggered oxidative stresses in leaves, as indicated by hydrogen peroxide accumulation and lipid peroxidation. Interestingly, these oxidative stresses were more evident in leaves simultaneously treated with both a high night temperature and MJ. The activity of the superoxide dismutase (SOD) was increased alongside the elevation of oxidative stress. Taken together, these results indicate that the combined treatment of a night temperature shift with MJ may accelerate anthocyanin production by increasing the levels of oxidative stress to the leaves of red leaf lettuce.

An Experimental Study of the Atmospheric Oxidation of a Biogenic Organic Compound (Methyl Jasmonate) in a Thin Water Film as in Fog or Aerosols

Experiments on the reactions of OH radicals in thin films of water were conducted in a photochemical reactor. The OH radical reactivity of a biogenic molecule (methyl jasmonate) was observed to be much larger in thin films of water than in the bulk aqueous phase. The pseudo-first order reaction rate was enhanced by an order of magnitude on a 38-micron film compared to the bulk liquid. However, the first order rate constant increased by 349%. This has implications in atmospheric systems like fog and mist which have large specific surface areas. The enhanced reactivity is attributable to both the partial solvation and faster diffusion at the air-water interface compared to the bulk liquid.

Effect of Postharvest Methyl Jasmonate Treatment on Early-Matured “Hass” Avocado Fruit Exocarp Colour Development during Ripening

Poor exocarp colour development is a common postharvest problem for early harvested “Hass” avocado fruit during ripening, which affects fruit quality and consumer preference. Therefore, measures to improve “Hass” avocado fruit colour developments are of great importance in the industry. This study investigated the effectiveness of postharvest methyl jasmonate treatment to improve early matured “Hass” avocado fruit exocarp colour during ripening. The results showed that T1 (10 μmol∙L−1) and T2 (100 μmol∙L−1) MeJA treatment increased visual colour, and decreased objective colour parameters (L*, C* and h˚) during ripening when compared with control fruit. Moreover, MeJA treated “Hass” avocado fruits had lower total chlorophyll content and higher total anthocyanin and cyanidin-3-O-glucoside concentration during ripening. In conclusion, “Hass” avocado fruit post-harvest treated with either T1 (10 μmol∙L−1) or T2 (100 μmol∙L−1) MeJA concentration improved exocarp quality attributes such as colour parameters (L*, C* h˚ and visual colour) and pigments (total anthocyanin and cyanidin-3-O-glucoside) during ripening, therefore, can be recommended for avocado fruit.

The Role of Jasmonates as Antibulbing Substances in the Bulb Formation of Onion

Onion plants form spherical bulbs under long-day conditions.Substances regulating bulb formation remain unknown.In the course of chemical studies on the bulb formation,α-linolenic acid was isolated from onion extracts as an antibulbing substance,the amount of which was synchronized with the bulb formation.Since allene oxide synthase inhibitor canceled the antibulbing activity ofα-linolenic acid,it was disclosed that jasmonic acid concerns this regulation.Structure-activity-relationship study revealed that its(3R,7S)stereochemistry is necessary for showing its antibulbing activity.It is concluded that(3R,7S)-jasmonate derived fromα-linolenic acid actually participates in the regulation of bulb formation.

MJ对抗旱性不同的两个玉米品种茎和根生长的影响

比较了ＭＪ对两个不同抗旱性玉米品种的影响．１～１００ｍｇ／ＬＭＪ对抗旱性较弱品种ＳＣ７０１幼苗株高有抑制效应．经ＭＪ处理的抗旱性强品种ＰＡＮ６０４３的株高、茎干重、根干重和根长皆高于ＳＣ７０１。

MJ·干旱·黄曲霉菌处理对花生种子发育过程中PI·PAC活性的影响

用1.0和0.1 mmol/L茉莉酸甲酯(MJ)处理花生结荚早期植株,结果表明:在不利条件(干旱、接种黄曲霉菌)下,不同浓度的MJ能诱导蛋白酶抑制剂(PI)活性的升高,且高浓度MJ处理比低浓度处理效果好。对苯丙氨酸解氨酶(PAL)活性的影响与PI活性相似,但是MJ诱导PAL活性的长期效果不明显。

Effect of Exogenous MJA Treatment of Tea Plants on the Growth of Geometrid Larvae

The effect of tea plant Camellia sinensis induced by exogenous methyl jasmonate （MJA） on lipoxygenase （LOX）, polyphenol oxidase （PPO） and proteinase inhibitor （PI） activity in the leaves of tea plants, as well as the growth and midgut proteinase activity of the geometrid Ectropis obliqua larvae were studied. MJA significantly induced LOX, PPO and PI activity in leaves of tea plants. When geometrid larvae have fed on leaves of tea plants treated with MJA, the activities of the high alkaline trypsin-like enzyme and chymotrypsin-like enzyme in their midgut were significantly inhibited, but the activities of the low alkaline trypsin-like enzyme in their midgut were unaffected, leading to imbalance between different types of proteinase activity in the midgut of the larvae and in turn, the growth were inhibited. These chains of response may be an important mechanism of the direct resistance induced by MJA-treatment of tea plant on geometrid larvae.

外源茉莉酸甲酯诱导脐橙果实抗青霉病与活性氧代谢的关系

【目的】通过探究外源茉莉酸甲酯(Methyl Jasmonate,MeJA)诱导脐橙果实采后抗青霉病与活性氧代谢的关系,为MeJA应用于控制果实采后病害提供理论依据。【方法】以纽荷尔和龙回红脐橙果实为试材,在温度(26±1)℃、湿度85%~95%的环境下,使用浓度为50μmol·L^(-1) MeJA密闭熏蒸处理24 h后,接种青霉病菌Penicillium italicum(1.25×10^(6) spores·mL^(-1))20µL,以无菌水处理为对照,另设100μmol·L^(-1)水杨苷异羟肟酸(salicyhydroxamic acid,SHAM)喷施处理,测定脐橙果实活性氧代谢相关指标并分析关键酶基因表达水平。【结果】50μmol·L^(-1) MeJA熏蒸处理显著提高2个脐橙品种果实的谷胱甘肽还原酶(GR)、抗坏血酸过氧化物酶(APX)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、单脱氢抗坏血酸还原酶(MDHAR)和脱氢抗坏血酸还原酶(DHAR)活性以及对应酶基因CsGR、CsAPX、Cu-ZnSOD、CsCAT、CsMDHAR、CsDHAR3的相对表达量,在纽荷尔中MeJA处理分别为对照的1.10、1.16、1.12、1.69、1.21、1.43、1.16、1.42、1.56、1.36、1.58、2.32倍,在龙回红中MeJA处理分别为对照的1.13、1.49、1.02、1.41、1.26、1.79、2.98、1.59、1.10、1.79、1.57、1.31倍;同时,显著提高了还原型谷胱甘肽(GSH)、抗坏血酸(AsA)含量和DPPH自由基清除率,降低了过氧化氢(H2O2)含量和超氧阴离子(O2.-)产生速率,延缓了丙二醛(MDA)含量的积累。【结论】使用MeJA于接种前24 h熏蒸脐橙果实,能提高柑橘果实活性氧代谢相关酶活性以及关键酶基因的表达量,增强果实对青霉病的抗性。

茉莉酸甲酯对盐胁迫下茼蒿种子萌发及幼苗抗性的影响

以小叶茼蒿种子为试材,采用培养皿萌发水培法,研究了不同施用浓度(0.02、0.20、2.0、20、200μmol·L^(-1))和浸泡时间(0、3、6、9、12、15 h)的外源茉莉酸甲酯(MeJA)对盐胁迫下茼蒿种子萌发指标(发芽率、发芽指数、发芽时间和芽长)、幼苗生长指标(根长、茎长、相对含水量)和幼苗生理指标(叶绿素、可溶性糖、可溶性蛋白质、脯氨酸含量)的影响,以期为茉莉酸甲酯在茼蒿种子耐盐萌发及幼苗栽培管理中的合理使用提供参考依据。结果表明:150 mmol·L^(-1)NaCl胁迫下,MeJA处理种子均可以不同程度缓解盐害,其中20μmol·L^(-1)MeJA溶液处理6 h为最佳处理浓度和时间,显著提高茼蒿种子的发芽率和发芽指数,而且对茼蒿幼苗生长的效果也较好,叶绿素含量及渗透调节物质含量增加。

外源茉莉酸甲酯对BNS366雄性育性的影响

为探究外源茉莉酸甲酯(methyl jasmonate,MeJA)对小麦温敏雄性不育系BNS366雄性育性的影响,以BNS366及其近等基因系郑麦366为试验材料,在正常秋季播种(2020年10月12日)和晚播(2020年12月2日)条件下,分别于2021年3月22日和3月31日开始至开花期前,设置0(清水,对照)、50、100、200、300、400和500μmol·L^(-1) MeJA(水溶液,喷施)7个处理,比较分析了不同处理间花粉可育率和自交结实率的差异。结果表明:在正常秋季播种条件下,郑麦366花粉可育率、国内法自交结实率和国际法自交结实率分别为86.31%、70.36%和112.22%,在晚播条件下分别为82.53%、92.53%和166.18%,均正常可育;在正常秋季播种条件下,200μmol·L^(-1) MeJA处理的国际法自交结实率为70.15%,比对照显著降低了42.07个百分点;在晚播条件下,200、300和500μmol·L^(-1) MeJA处理的国内法自交结实率分别为74.71%、74.01%和73.45%,比对照显著降低了17.82、18.52和19.08个百分点;在两个播期下,郑麦366其他浓度MeJA处理的上述3个指标与对照差异均不显著。在正常秋季播种条件下,BNS366花粉可育率为零,达到全不育水平,国内法自交结实率和国际法自交结实率均为0.24%,100μmol·L^(-1) MeJA处理的花粉可育率为88.25%,达到正常可育水平,国内法自交结实率和国际法自交结实率分别为56.41%和94.01%,能正常结实,与对照差异达到显著水平;在晚播条件下,BNS366的上述3个指标分别为51.72%、41.23%和93.08%,正常可育,100μmol·L^(-1) MeJA处理的国际法自交结实率为39.72%,比对照显著降低了53.36个百分点;在两个播期下,BNS366其他浓度MeJA处理的上述3个指标与对照差异均不显著。由此说明,在2020-2021年,外源MeJA对郑麦366和BNS366可育植株的雄性育性可能具有降低效应,对BNS366不育植株的雄性不育性具有较强恢复效应。

外源茉莉酸甲酯诱导大麦叶斑病抗性研究

【目的】探讨不同浓度外源茉莉酸甲酯(MeJA)诱导大麦抗叶斑病效应差异及其分子机制,为应用MeJA防治大麦叶斑病提供理论依据。【方法】以‘蒙啤麦3号’大麦品种幼苗为材料,设置不接菌(无菌水处理叶片)、接菌(无菌水处理叶片接种麦根腐平脐蠕孢菌)和接菌+MeJA(0.5,1.0,1.5,2.0,2.5mmol/LMeJA喷施叶片后接菌)3组处理,于三叶期调查叶斑病发病情况,并据此筛选最适MeJA浓度,然后测定不接菌、接菌及接菌+MeJA(最适浓度)下不同处理时间叶片的抗氧化酶、抗病相关酶活性、丙二醛含量、渗透调节物质含量以及相关基因表达水平。【结果】(1)叶面喷施外源MeJA提高了大麦对叶斑病的抗性,1.5mmol/LMeJA处理叶片的病情指数较对照显著降低19.03%,诱导抗性效果最佳;(2)与单独接菌处理相比,1.5mmol/LMeJA处理大麦叶片超氧化物歧化酶、过氧化物酶、过氧化氢酶、几丁质酶和β-1,3-葡聚糖酶活性均显著提高,而其丙二醛、脯氨酸、可溶性糖和可溶性蛋白含量显著降低,同时受MeJA调控转录因子及编码抗病相关酶的基因表达量显著上调。【结论】外源喷施1.5mmol/LMeJA通过调节抗病相关酶活性和渗透调节物质含量,以及调控抗病相关酶基因及茉莉酸信号途径关键转录因子基因表达,进而提高大麦植株的叶斑病抗性。

外源施用茉莉酸甲酯对番茄果实挥发性风味成分及含量的影响

挥发性物质是番茄果实风味品质的重要组成部分,其是否响应外源茉莉酸甲酯(methyl jasmonate,MeJA)的调控尚不明确。以‘朝研298’为实验材料,设定1、10、100、200μmol/L 4个MeJA浓度,以等体积超纯水为对照(CK),在番茄果实采收前喷施2次。待果实成熟时采用顶空-固相微萃取-气相色谱-质谱联用技术测定其挥发性成分,结合多元统计分析法明确改善番茄果实风味品质的MeJA最佳浓度。结果表明:5个处理组番茄果实中共检出71种挥发性组分,包括23种醛类、17种醇类、8种酮类、7种酯类、6种烃类和10种其他类物质,与对照组相比,外源喷施MeJA可提高番茄果实挥发性物质的种类数和总含量,且随着浓度增大呈先升高后降低的趋势,100μmol/L MeJA处理时达到最大值;各处理组含有33种共有物质,含量最高的成分均为顺-3-己烯-1-醇;所有番茄果实中共筛选出13种特征香气成分,主要呈现青香、果香和花香气味。研究结果为MeJA改善番茄果实风味品质提供理论支撑,有利于进一步探究番茄果实挥发性物质合成调控机理。

基于转录组分析茉莉酸甲酯诱导猕猴桃果实抗采后软腐病作用机制

【目的】茉莉酸甲酯(MeJA)作为生物信号分子广泛存在于植物体内,可激发果实抵御生物或非生物因子胁迫,在贮藏保鲜中发挥重要作用。转录组技术已广泛应用于探索采后病害防治的相关调控机制,它能够在分子水平上深入阐明病原菌与植物之间互作的机制,有利于鉴定和挖掘胁迫响应基因。前期研究表明,MeJA能够通过调控防御系统诱导猕猴桃果实抗采后软腐病,并分析了苯丙烷途径、膜脂代谢以及能量代谢与MeJA诱导猕猴桃果实抗软腐病的关系,从生理和分子水平初步阐述了MeJA介导的猕猴桃果实抗病途径。基于茉莉酸甲酯(MeJA)诱导猕猴桃果实抵御软腐病菌(Botryosphaeria dothidea)的转录组数据,分析猕猴桃果实响应病原侵染过程中抗病相关基因的表达,为研究MeJA介导的猕猴桃抗软腐病的分子机制提供理论参考。【方法】以‘红阳’猕猴桃为试验材料,选择接种B.dothidea组和MeJA处理并接种组,将0,1,5 d的猕猴桃样本进行转录组测序。以log2FC≥2,Qvalue≤0.05和FPKM>2为标准筛选差异表达基因,进行实时荧光定量PCR验证,并进行GO功能注释和KEGG通路富集分析。【结果】转录组测序共获得约114.97 GB数据,各样本Clean data均达到6.39 GB,Q30碱基百分比在90.41%以上。对各个时期差异表达基因数量统计发现,猕猴桃果实DEGs随着B.dothidea侵染时间延长逐渐增加;而响应MeJA诱导的DEGs主要基中在预处理时,接种病原菌后DEGs数量逐渐减少,表明外源MeJA在早期诱导了大量基因参与果实抗病性。未处理接种组和MeJA处理接种组在0,1,5 d共同差异表达基因分别为164个和314个。GO富集结果表明,差异表达基因主要与生物过程中的细胞过程、代谢过程、对刺激的反应和生物调节,细胞组分中的细胞解剖实体和胞内,以及分子功能中的结合、催化活性和转录调节活性相关。KEGG通路富集结果表明,猕猴桃果实经MeJA处理后接种B.dothidea,其植物-病原互作途径、α-亚麻酸代谢途径、谷胱甘肽代谢途径、苯并恶嗪类生物合成途径、抗坏血酸和醛酸代谢途径特有富集;苯丙烷生物合成途径、芪类化合物、二芳基庚烷和姜醇生物合成途径、类黄酮生物合成途径和MAPK信号通路在两个处理组中共同富集。共筛选出了31个同时响应MeJA和B.dothidea的差异表达基因,推测这些基因与MeJA介导的抗病过程有关。【结论】对外源MeJA处理的猕猴桃果实在接种B.dothidea后的不同时期(0,1,5 d)进行RNASeq分析,结果显示MeJA诱导猕猴桃果实抵御软腐病过程中,大量的生理代谢途径的基因发生变化,有助于增强果实信号分子对防御系统的启动,提高抗菌物质的合成和抗氧化能力,进而提高了猕猴桃果实的抗病性,延长其贮藏时间。