Objective:To investigate variable number tandem repeat (VNTR) polymorphism of the 17th intron of Rb gene in Shaanxi aged population and the relationship between the polymorphism of Rb gene and atherosclerosis(AS) gene...Objective:To investigate variable number tandem repeat (VNTR) polymorphism of the 17th intron of Rb gene in Shaanxi aged population and the relationship between the polymorphism of Rb gene and atherosclerosis(AS) genetic suscepti- bility. Methods: VNTR polymorphism of the 17th intron of Rb gene were examined in 100 Shaanxi aged AS patients and 100 Shaanxi aged control individuals by PCR-Rb-Xba Ⅰ-RFLP. Results::Two alleles were found both in AS group and control group, which were separately 945 bp(S1) and 630bp + 315bp(S2). S1S2 genotype was the most frequent one in the two populations. Significant difference in allele frequency was not found between AS group and control group, and allele frequency was no significant difference between Chinese and Caucasian. Conclusion: Xba Ⅰ enzyme site of Rb gene could have been certainly stable in AS population, and it was inferred that the polymorphism locus was not liable to cause mutation, which might not implicated in the formation of AS.展开更多
The title complex [AgL（NO3）]2 1, where L = 2-acetamido-5-methyl-1,3,4-thiadiazole, was prepared from the reaction of 2-acetamido-5-methyl-1,3,4-thiadiazole with AgNO3 at room temperature and its structure has been d...The title complex [AgL（NO3）]2 1, where L = 2-acetamido-5-methyl-1,3,4-thiadiazole, was prepared from the reaction of 2-acetamido-5-methyl-1,3,4-thiadiazole with AgNO3 at room temperature and its structure has been determined by single-crystal X-ray diffraction. The crystal is of monoclinic, space group P2t/c with a = 12.5454（8）, b = 11.9672（12）, c = 6.8925（6） A, β = 98.468（2）°. V= 1023.51（15） A^3, Mr= 3127.08, Dc = 2.123 g/cm^3, Z= 4, μ（MoKoσ） = 2.174 mm^-1, F（000） = 640, R = 0.0259 and wR = 0.0580 for 1787 observed reflections with I 〉 2σ（I）. Complex 1 self-assembls into a quasi-three-dimensional network consisting of adjacent dimers through intermolecular Ag...O weak interactions.展开更多
目的:应用两种不同方法检测系统性红斑狼疮(SLE)患者CD4^+T细胞p16基因甲基化状态,比较两种检测方法的差异。方法:采用以Taqman探针为基础的MSP法(方法1)和以SYBR Green Ⅰ为基础的MSP法(方法2)分别检测40例SLE患者和20例正常人CD4^+T...目的:应用两种不同方法检测系统性红斑狼疮(SLE)患者CD4^+T细胞p16基因甲基化状态,比较两种检测方法的差异。方法:采用以Taqman探针为基础的MSP法(方法1)和以SYBR Green Ⅰ为基础的MSP法(方法2)分别检测40例SLE患者和20例正常人CD4^+T细胞中p16基因启动子区甲基化状态。结果:Taqman探针方法的结果为:SLE患者CD4^+T细胞p16基因甲基化阳性率(35.7%,10/28)高于对照组(10%,2/20),两者比较差异有统计学意义(x^2=4.11,P<0.05)。SYBR Green Ⅰ方法的结果为:患者组和对照组的CD4^+T细胞的p16基因均呈高甲基化状态,应用t检验分析发现P>0.05,二者无统计学差异。结论:Taqman探针法消除了引物二聚体和非特异性扩增对试验结果的影响,提高了结果的特异性和准确性,被证明是进行DNA甲基化状态检测的可靠方法。展开更多
基金National Natural Sciences Foundation of China No. 39700165
文摘Objective:To investigate variable number tandem repeat (VNTR) polymorphism of the 17th intron of Rb gene in Shaanxi aged population and the relationship between the polymorphism of Rb gene and atherosclerosis(AS) genetic suscepti- bility. Methods: VNTR polymorphism of the 17th intron of Rb gene were examined in 100 Shaanxi aged AS patients and 100 Shaanxi aged control individuals by PCR-Rb-Xba Ⅰ-RFLP. Results::Two alleles were found both in AS group and control group, which were separately 945 bp(S1) and 630bp + 315bp(S2). S1S2 genotype was the most frequent one in the two populations. Significant difference in allele frequency was not found between AS group and control group, and allele frequency was no significant difference between Chinese and Caucasian. Conclusion: Xba Ⅰ enzyme site of Rb gene could have been certainly stable in AS population, and it was inferred that the polymorphism locus was not liable to cause mutation, which might not implicated in the formation of AS.
基金supported by the National Natural Science Foundation of China (Grants No.21561013,21501077)the Jiangxi Provincial Natural Science Foundation of China (Grants No.20171BAB203005,20161ACB21013,20171BCB23066)+2 种基金the Major Project of Jiangxi Provincial Education Department of China (Grant No.GJJ160597)the Foundation of State Key Laboratory of Structural Chemistry (Grant No.20180019)the Program for Qingjiang Excellent Young Talents of Jiangxi University of Science and Technology~~
文摘The title complex [AgL（NO3）]2 1, where L = 2-acetamido-5-methyl-1,3,4-thiadiazole, was prepared from the reaction of 2-acetamido-5-methyl-1,3,4-thiadiazole with AgNO3 at room temperature and its structure has been determined by single-crystal X-ray diffraction. The crystal is of monoclinic, space group P2t/c with a = 12.5454（8）, b = 11.9672（12）, c = 6.8925（6） A, β = 98.468（2）°. V= 1023.51（15） A^3, Mr= 3127.08, Dc = 2.123 g/cm^3, Z= 4, μ（MoKoσ） = 2.174 mm^-1, F（000） = 640, R = 0.0259 and wR = 0.0580 for 1787 observed reflections with I 〉 2σ（I）. Complex 1 self-assembls into a quasi-three-dimensional network consisting of adjacent dimers through intermolecular Ag...O weak interactions.
文摘目的:应用两种不同方法检测系统性红斑狼疮(SLE)患者CD4^+T细胞p16基因甲基化状态,比较两种检测方法的差异。方法:采用以Taqman探针为基础的MSP法(方法1)和以SYBR Green Ⅰ为基础的MSP法(方法2)分别检测40例SLE患者和20例正常人CD4^+T细胞中p16基因启动子区甲基化状态。结果:Taqman探针方法的结果为:SLE患者CD4^+T细胞p16基因甲基化阳性率(35.7%,10/28)高于对照组(10%,2/20),两者比较差异有统计学意义(x^2=4.11,P<0.05)。SYBR Green Ⅰ方法的结果为:患者组和对照组的CD4^+T细胞的p16基因均呈高甲基化状态,应用t检验分析发现P>0.05,二者无统计学差异。结论:Taqman探针法消除了引物二聚体和非特异性扩增对试验结果的影响,提高了结果的特异性和准确性,被证明是进行DNA甲基化状态检测的可靠方法。
