As an important wild blueberry resource,Vaccinium uliginosum has attracted more and more attention.At present,the wild resources are under destruction.The conservation of wild Vaccinium uliginosum resources is imminen...As an important wild blueberry resource,Vaccinium uliginosum has attracted more and more attention.At present,the wild resources are under destruction.The conservation of wild Vaccinium uliginosum resources is imminent.However,there are few researches on the protection and preservation of its germplasm resources.In vitro preservation is an important method for germplasm conservation.In this study,one strain of wild Vaccinium uliginosum was used as material.The effects of temperature(25℃,15℃,10℃,or 0℃),media(WPM,1/2WPM or 1/3WPM),medium supplements(sorbitol or mannose),and photoperiod(8,10,12,or 14 h•d^(-1))on the growth,survival rate and rejuvenation rate of the plantlets were studied.The physiological changes of plantlets during preservation were analyzed.Methylation-sensitive amplified polymorphism(MSAP)analysis of genomic DNA methylation of plantlets was carried out to explore the genetic stability of the plantlets after preservation.The research results provided a theoretical basis for the germplasm preservation of Vaccinium uliginosum.展开更多
DNA methylation plays an important role in the epigenetic regulation of gene expression during plant growth,development,and polyploidization.However,there is still no distinct evidence in tobacco regarding the distrib...DNA methylation plays an important role in the epigenetic regulation of gene expression during plant growth,development,and polyploidization.However,there is still no distinct evidence in tobacco regarding the distribution of the methylation pattern and whether it contributes to qualitative characteristics.We studied the levels and patterns of methylation polymorphism at CCGG sites in 48 accessions of allotetraploid flue-cured tobacco,Nicotiana tabacum,using a methylation-sensitive amplified polymorphism(MSAP) technique.The results showed that methylation existed at a high level among tobacco accessions,among which 49.3% sites were methylated and 69.9% allelic sites were polymorphic.A cluster analysis revealed distinct patterns of geography-specific groups.In addition,three polymorphic sites significantly related to tobacco mosaic virus(TMV) resistance were explored.This suggests that tobacco breeders should pay more attention to epigenetic traits.展开更多
Persimmon （ Diospyros kaki Thunb. ） , a member of Ebenaceae family, is known as ＂woody grain＂ and ＂iron-stem crop＂. However, the epigenetie research has been rarely reported. In this study, tannin content in lat...Persimmon （ Diospyros kaki Thunb. ） , a member of Ebenaceae family, is known as ＂woody grain＂ and ＂iron-stem crop＂. However, the epigenetie research has been rarely reported. In this study, tannin content in late fruit development periods was determined. Moreover, the MSAP （methylation-sensitive amplified polymorphism） method was used to analyze methylation profile, and 13 primer combinations were selected. In total, 5 differential fragments were cloned and sequenced. This research would be helpful for further gene cloning, research on deastringency mechanism, as well as molecular breeding of D. kaki.展开更多
基金Supported by the National Natural Science Foundation of China(32172521)。
文摘As an important wild blueberry resource,Vaccinium uliginosum has attracted more and more attention.At present,the wild resources are under destruction.The conservation of wild Vaccinium uliginosum resources is imminent.However,there are few researches on the protection and preservation of its germplasm resources.In vitro preservation is an important method for germplasm conservation.In this study,one strain of wild Vaccinium uliginosum was used as material.The effects of temperature(25℃,15℃,10℃,or 0℃),media(WPM,1/2WPM or 1/3WPM),medium supplements(sorbitol or mannose),and photoperiod(8,10,12,or 14 h•d^(-1))on the growth,survival rate and rejuvenation rate of the plantlets were studied.The physiological changes of plantlets during preservation were analyzed.Methylation-sensitive amplified polymorphism(MSAP)analysis of genomic DNA methylation of plantlets was carried out to explore the genetic stability of the plantlets after preservation.The research results provided a theoretical basis for the germplasm preservation of Vaccinium uliginosum.
基金supported by the Program for High-Quality Tobacco Development of China (No. [2010]221)the Foundation of Science and Technology of Guizhou Province (No. J[2010]2251)the Program for Guizhou Tobacco Science of China (No. 200910)
文摘DNA methylation plays an important role in the epigenetic regulation of gene expression during plant growth,development,and polyploidization.However,there is still no distinct evidence in tobacco regarding the distribution of the methylation pattern and whether it contributes to qualitative characteristics.We studied the levels and patterns of methylation polymorphism at CCGG sites in 48 accessions of allotetraploid flue-cured tobacco,Nicotiana tabacum,using a methylation-sensitive amplified polymorphism(MSAP) technique.The results showed that methylation existed at a high level among tobacco accessions,among which 49.3% sites were methylated and 69.9% allelic sites were polymorphic.A cluster analysis revealed distinct patterns of geography-specific groups.In addition,three polymorphic sites significantly related to tobacco mosaic virus(TMV) resistance were explored.This suggests that tobacco breeders should pay more attention to epigenetic traits.
基金Supported by College Students’Innovation and Entrepreneurship Training Program of Huanggang Normal University(zx201425)Fund of Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains(2015TD07)
文摘Persimmon （ Diospyros kaki Thunb. ） , a member of Ebenaceae family, is known as ＂woody grain＂ and ＂iron-stem crop＂. However, the epigenetie research has been rarely reported. In this study, tannin content in late fruit development periods was determined. Moreover, the MSAP （methylation-sensitive amplified polymorphism） method was used to analyze methylation profile, and 13 primer combinations were selected. In total, 5 differential fragments were cloned and sequenced. This research would be helpful for further gene cloning, research on deastringency mechanism, as well as molecular breeding of D. kaki.
